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LPAR5, GNAT3 and partial amino acid transporters messenger RNA expression patterns in digestive tracts, metabolic organs and muscle tissues of growing goats

Published online by Cambridge University Press:  31 October 2018

X. Zhu Open the ORCID record for X. Zhu [Opens in a new window] ,
J. Jiao ,
C. Zhou ,
S. Tang ,
M. Wang ,
J. Kang ,
X. Han  and
Z. Tan
X. Zhu
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China University of Chinese Academy of Sciences, Beijing 100049, P. R. China
J. Jiao
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
C. Zhou
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
S. Tang
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
M. Wang
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
J. Kang
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
X. Han
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
Z. Tan*
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P. R. China University of Chinese Academy of Sciences, Beijing 100049, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan 410128, P.R. China
*
†E-mail: [email protected]
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Abstract

Sufficient amino acid (AA) transport is essential to ensure the normal physiological function and growth of growing animals. The processes of AA sensing and transport in humans and murine animals, but rarely in goats, have been arousing great interest recently. This study was conducted to investigate the messenger RNA expression patterns of lysophosphatidic acid receptor 5 (LPAR5), guanine nucleotide-binding protein α-transducing 3 (GNAT3) and important partial AA transporters in digestive tracts, metabolic organs and muscles of growing goats. The results showed that these genes were widely expressed in goats, and had different expression patterns. LPAR5, GNAT3, solute carrier (SLC38A2), SLC7A7, SLC7A1 and SLC3A1 were rarely expressed in the rumen, but were highly expressed in the abomasum and intestine which are the main sites of AA absorption. GNAT3, SLC38A1, SLC38A2, SLC6A19, SLC7A7 and SLC7A1 showed comparatively high expression in the pancreas and the vital digestive glands, and the relatively high expression of these nine genes were noted in the tibialis posterior, the active muscle in energy metabolism. The correlation analysis showed that there were certain positive correlation among most genes. The current results indicate that the AA sensing and transport occur extensively in the abomasum and small intestine, metabolic organs and muscle tissues of ruminants, and that related genes have tissue specificity.

Keywords

transcriptionlysophosphatidic acid receptor 5guanine nucleotide-binding protein α-transducing 3amino acid transportingCapra hircus
Type
Research Article
Information
animal , Volume 13 , Issue 7 , July 2019 , pp. 1394 - 1402
Copyright
© The Animal Consortium 2018 

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