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Leucine regulates α-amylase and trypsin synthesis in dairy calf pancreatic tissue in vitro via the mammalian target of rapamycin signalling pathway

Published online by Cambridge University Press:  08 January 2019

L. Guo ,
J. H. Yao ,
C. Zheng ,
H. B. Tian ,
Y. L. Liu ,
S. M. Liu ,
C. J. Cai ,
X. R. Xu  and
Y. C. Cao Open the ORCID record for Y. C. Cao [Opens in a new window]
L. Guo
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
J. H. Yao
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
C. Zheng
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
H. B. Tian
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
Y. L. Liu
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
S. M. Liu
Affiliation:
School of Animal Biology, University of Western Australia, Crawley, WA 6009, Australia
C. J. Cai
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
X. R. Xu
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
Y. C. Cao*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, People’s Republic of China
*
†E-mail: [email protected]
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Abstract

Starch digestion in the small intestines of the dairy cow is low, to a large extent, due to a shortage of syntheses of α-amylase. One strategy to improve the situation is to enhance the synthesis of α-amylase. The mammalian target of rapamycin (mTOR) signalling pathway, which acts as a central regulator of protein synthesis, can be activated by leucine. Our objectives were to investigate the effects of leucine on the mTOR signalling pathway and to define the associations between these signalling activities and the synthesis of pancreatic enzymes using an in vitro model of cultured Holstein dairy calf pancreatic tissue. The pancreatic tissue was incubated in culture medium containing l-leucine for 3 h, and samples were collected hourly, with the control being included but not containing l-leucine. The leucine supplementation increased α-amylase and trypsin activities and the messenger RNA expression of their coding genes (P <0.05), and it enhanced the mTOR synthesis and the phosphorylation of mTOR, ribosomal protein S6 kinase 1 and eukaryotic initiation factor 4E-binding protein 1 (P <0.05). In addition, rapamycin inhibited the mTOR signal pathway factors during leucine treatment. In sum, the leucine regulates α-amylase and trypsin synthesis in dairy calves through the regulation of the mTOR signal pathways.

Keywords

leucinemammalian target of rapamycindigestive enzymespancreas
Type
Research Article
Information
animal , Volume 13 , Issue 9 , September 2019 , pp. 1899 - 1906
Copyright
© The Animal Consortium 2019 

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