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Length of concentrate finishing affects the fatty acid composition of grass-fed and genetically lean beef: an emphasis on trans-18:1 and conjugated linoleic acid profiles

Published online by Cambridge University Press:  26 April 2011

N. Aldai ,
M. E. R. Dugan ,
J. K. G. Kramer ,
A. Martínez ,
O. López-Campos ,
A. R. Mantecón  and
K. Osoro
N. Aldai*
Affiliation:
Lacombe Research Centre, AAFC, 6000 C&E Trail, Lacombe T4L 1W1, Alberta, Canada
M. E. R. Dugan
Affiliation:
Lacombe Research Centre, AAFC, 6000 C&E Trail, Lacombe T4L 1W1, Alberta, Canada
J. K. G. Kramer
Affiliation:
Guelph Food Research Centre, AAFC, Guelph N1G 5C9, Ontario, Canada
A. Martínez
Affiliation:
Sistemas de Producción Animal, SERIDA, Apdo. 13 – 33300 Villaviciosa, Asturias, Spain
O. López-Campos
Affiliation:
Lacombe Research Centre, AAFC, 6000 C&E Trail, Lacombe T4L 1W1, Alberta, Canada
A. R. Mantecón
Affiliation:
Instituto de Ganadería de Montaña, CSIC-ULE, Finca Marzanas, 24346 Grulleros, León, Spain
K. Osoro
Affiliation:
Sistemas de Producción Animal, SERIDA, Apdo. 13 – 33300 Villaviciosa, Asturias, Spain
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Abstract

Intensively finishing cattle on a high-grain diet is generally used to enhance marbling, whereas extensively finishing on grass is known to provide improved muscle fatty acid profiles. The objective of this study was to evaluate to what extent intensive concentrate finishing (0, 1 or 2 months) can be combined with forage feeding without negatively affecting the fatty acid profile of genetically lean animals. Bulls from the ‘Asturiana de los Valles’ breed were reared under grazing conditions with/without final finishing on a barley-based concentrate: 0 months (control; n = 7), 1 month (n = 10) and 2 months (n = 7). Yearling bulls were slaughtered commercially at an average live weight of 516 ± 9.8 kg. Increasing the finishing time on concentrate significantly increased the saturated and monounsaturated fatty acids, whereas polyunsaturated fatty acids (PUFAs) tended to decrease and it was not possible to increase the long-chain PUFA content in muscle tissue of this breed. An increase was observed for total trans-18:1 (average 5.5% with grain v. 3.7% for grass). The 11t-18:1/10t-18:1 ratio was significantly higher in grass-fed (average 8.1) compared with grain-finished animals (average 1.1). Grass or limited concentrate finishing reduced the n-6/n-3 ratio in muscle tissue (average 3.6 for 0 and 1 month, and 4.9 for 2 months on grain finishing). The beef was within or close to the recommended values for human consumption (i.e. polyunsaturated/saturated > 0.45, n-6/n-3 < 4.0), and total trans-FA content was low. However, finishing increased the content of undesirable trans-18:1 and conjugated linoleic acid isomers, particularly after 2 months, whereas grass finishing was judged to provide a healthier beef fatty acid profile.

Keywords

beefCLAconcentrategrassvaccenic acid
Type
Full Paper
Information
animal , Volume 5 , Issue 10 , 26 August 2011 , pp. 1643 - 1652
Copyright
Copyright © The Animal Consortium 2011

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References

Aldai, N, Nájera, AI, Dugan, MER, Celaya, R, Osoro, K 2007a. Characterisation of intramuscular, intermuscular and subcutaneous adipose tissues in yearling bulls of different genetic groups. Meat Science 76, 682–691.CrossRefGoogle ScholarPubMed
Aldai, N, Nájera, AI, Martínez, A, Celaya, R, Osoro, K 2007b. Correlation between carcass conformation and fat cover degree, and muscle fatty acid profile of yearling bulls depending on breed and mh-genotype. Livestock Science 107, 199–212.CrossRefGoogle Scholar
Aldai, N, Dugan, MER, Nájera, AI, Osoro, K 2008a. N-6 and n-3 fatty acids in different beef adipose tissues depending on the presence or absence of the gene responsible for double-muscling. Czech Journal of Animal Science 53, 515–522.CrossRefGoogle Scholar
Aldai, N, Dugan, MER, Kramer, JKG, Mir, PS, McAllister, TA 2008b. Nonionophore antibiotics do not affect the trans-18:1 and conjugated linoleic acid composition in beef adipose tissue. Journal of Animal Science 86, 3522–3532.CrossRefGoogle ScholarPubMed
Aldai, N, Dugan, MER, Juárez, M, Martínez, A, Osoro, K 2010a. Double-muscling character influences the trans-18:1 and conjugated linoleic acid profiles in concentrate-fed yearling bulls. Meat Science 85, 59–65.CrossRefGoogle Scholar
Aldai, N, Dugan, MER, Kramer, JKG, Robertson, WM, Juárez, M, Aalhus, JL 2010b. Trans-18:1 and conjugated linoleic acid profiles after the inclusion of buffer, sodium sesquicarbonate, in the concentrate of finishing steers. Meat Science 84, 735–741.CrossRefGoogle Scholar
Aldai, N, Murray, BE, Oliván, M, Martínez, A, Troy, DJ, Osoro, K, Nájera, AI 2006. The influence of breed and mh-genotype on carcass conformation, meat physico-chemical characteristics, and the fatty acid profile of muscle from yearling bulls. Meat Science 72, 486–495.CrossRefGoogle ScholarPubMed
Alfaia, CPM, Alves, SP, Martins, SIV, Costa, ASH, Fontes, CMGA, Lemos, JPC, Bessa, RJB, Prates, JAM 2009. Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability. Food Chemistry 114, 939–946.CrossRefGoogle Scholar
Arthur, PF 1995. Double muscling in cattle: a review. Australian Journal of Agricultural Research 46, 1493–1516.CrossRefGoogle Scholar
Bassett, CMC, Edel, AL, Patenaude, AF, McCullough, RS, Blackwood, DP, Chouinard, PY, Paquin, P, Lamarche, B, Pierce, GN 2010. Dietary vaccenic acid has antiatherogenic effects in LDLr−/−mice. Journal of Nutrition 140, 18–24.CrossRefGoogle ScholarPubMed
Bauchart, D, Roy, A, Lorenz, S, Chardigny, JM, Ferlay, A, Gruffat, D, Sébédio, JL, Chilliard, Y, Durand, D 2007. Butters varying in trans 18: 1 and cis-9, trans-11 conjugated linoleic acid modify plasma lipoproteins in the hypercholesterolemic rabbit. Lipids 42, 123–133.CrossRefGoogle ScholarPubMed
Bessa, RJB, Santos-Silva, J, Ribeiro, JMR, Portugal, AV 2000. Reticulo-rumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers. Livestock Production Science 63, 201–211.CrossRefGoogle Scholar
Bessa, RJB, Alves, SP, Jerónimo, E, Alfaia, CM, Prates, JAM, Santos-Silva, J 2007. Effect of lipid supplements on ruminal biohydrogenation intermediates and muscle fatty acids in lambs. European Journal of Lipid Science and Technology 109, 868–878.CrossRefGoogle Scholar
Bessa, RJB, Alves, SP, Figueiredo, R, Teixeira, AJR, Rodrigues, AIP, Janeiro, A, Costa, M, Santos-Silva, J, Prates, JAM 2006. Discrimination of production system and origin of animal products using chemical markers. In Animal products from the Mediterranean area (ed. JMC Ramalho Ribeiro, AEM Horta, C Mosconi and A Rosati), EAAP Publication No. 119, pp. 231–240. Wageningen Academic Publishers, Wageningen, The Netherlands.CrossRefGoogle Scholar
Collomb, M, Bütikofer, U, Sieber, R, Jeangros, B, Bosset, JO 2002. Correlation between fatty acids in cows’ milk fat produced in the Lowlands, Mountains and Highlands of Switzerland and botanical composition of the fodder. International Dairy Journal 12, 661–666.CrossRefGoogle Scholar
Commission Regulation 1981. No 2930/81 of 12 October 1981 adopting additional provisions for the application of the Community scale for the classification of carcasses of adult bovine animals. Official Journal L293, 6–7.Google Scholar
Council Regulation 1981. No 1208/81 of 28 April 1981 determining the Community scale for the classification of carcasses of adult bovine animals. Official Journal L123, 3–6.Google Scholar
Cruz-Hernandez, C, Deng, Z, Zhou, J, Hill, AR, Yurawecz, MP, Delmonte, P, Mossoba, MM, Dugan, MER, Kramer, JKG 2004. Methods for analysis of conjugated linoleic acids and trans-18:1 isomers in dairy fats by using a combination of gas chromatography, silver-ion thin-layer chromatography/gas chromatography, and silver-ion liquid chromatography. Journal of AOAC International 87, 545–562.CrossRefGoogle Scholar
Cruz-Hernandez, C, Kramer, JKG, Kraft, J, Santercole, V, Or-Rashid, M, Deng, Z, Dugan, MER, Delmonte, P, Yurawecz, MP 2006. Systematic analysis of trans and conjugated linoleic acids in the milk and meat of ruminants. In Advances in conjugated linoleic acid research, volume 3 (ed. MP Yurawecz, JKG Kramer, O Gudmundsen, MW Pariza and S Banni), pp. 45–93. AOCS Press, Champaign, IL, USA.Google Scholar
Dannenberger, D, Nuernberg, K, Nuernberg, G, Scollan, N, Steinhart, H, Ender, K 2005. Effect of pasture vs. concentrate diet on CLA isomer distribution in different tissue lipids of beef cattle. Lipids 40, 589–598.CrossRefGoogle ScholarPubMed
Destaillats, F, Trottier, JP, Galvez, JMG, Angers, P 2005. Analysis of α-linolenic acid biohydrogenation intermediates in milk fat with emphasis on conjugated linolenic acids. Journal of Dairy Science 88, 3231–3239.CrossRefGoogle ScholarPubMed
Dewhurst, RJ, Scollan, ND, Youell, SJ, Tweed, JKS, Humphreys, MO 2001. Influence of species, cutting date and cutting interval on the fatty acid composition of grasses. Grass and Forage Science 56, 68–74.CrossRefGoogle Scholar
Dugan, MER, Rolland, DC, Aalhus, JL, Aldai, N, Kramer, JKG 2008. Subcutaneous fat composition of youthful and mature Canadian beef: emphasis on individual conjugated linoleic acid and trans-18:1 isomers. Canadian Journal of Animal Science 88, 591–599.CrossRefGoogle Scholar
Dugan, MER, Kramer, JKG, Robertson, WM, Meadus, WJ, Aldai, N, Rolland, DC 2007. Comparing subcutaneous adipose tissue in beef and muskox with emphasis on trans 18:1 and conjugated linoleic acids. Lipids 42, 509–518.CrossRefGoogle Scholar
Duckett, SK, Wagner, DG, Yates, LD, Dolezal, HG, May, SG 1993. Effects of time on feed on beef nutrient composition. Journal of Animal Science 71, 2079–2088.CrossRefGoogle ScholarPubMed
Enser, M, Hallett, KG, Hewett, B, Fursey, GAJ, Wood, JD, Harrington, G 1998. Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Science 49, 329–341.CrossRefGoogle ScholarPubMed
Ferdinandusse, S, Rusch, H, van Lint, AE, Dacremont, G, Wanders, RJ, Vreken, P 2002. Stereochemistry of the peroxisomal branched-chain fatty acid alpha- and betaoxidation systems in patients suffering from different peroxisomal disorders. Journal of Lipid Research 43, 438–444.CrossRefGoogle ScholarPubMed
Field, CJ, Blewett, HH, Proctor, S, Vine, D 2009. Human health benefits of vaccenic acid. Applied Physiology, Nutrition, and Metabolism 34, 979–991.CrossRefGoogle ScholarPubMed
Fraser, MD, Davies, DA, Vale, JE, Nute, GR, Hallett, KG, Richardson, RI, Wright, IA 2009. Performance and meat quality of native and continental cross steers grazing improved upland pasture or semi-natural rough grazing. Livestock Science 123, 70–82.CrossRefGoogle Scholar
Fukuda, S, Nakanishi, Y, Chikayama, E, Ohno, H, Hino, T, Kikuchi, J 2009. Evaluation and characterization of bacterial metabolic dynamics with a novel profiling technique, real-time metabolotyping. PloS One 4 (e4893), 1–10.CrossRefGoogle ScholarPubMed
Gleissman, H, Yang, R, Martinod, K, Lindskog, M, Serhan, CN, Johnsen, JI, Kogner, P 2010. Docosahexaenoic acid metabolome in neural tumors: identification of cytotoxic intermediates. The FASEB Journal 24, 906–915.CrossRefGoogle ScholarPubMed
Gomez-Cortes, P, Tyburczy, C, Brenna, JT, Juarez, M, De La Fuente, MA 2009. Characterization of cis-9 trans-11 trans-15 C18:3 in milk fat by gas chromatography and covalent adduct chemical ionization tandem mass spectrometry. The Journal of Lipid Research 50, 2412–2420.CrossRefGoogle Scholar
Grobet, L, Poncelet, D, Royo, JL, Brouwers, B, Pirottin, D, Michaux, C, Ménissier, F, Zanotti, M, Dunner, S, Georges, M 1998. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mammalian Genome 9, 210–213.CrossRefGoogle ScholarPubMed
Harfoot, CG, Hazlewood, GP 1997. Lipid metabolism in the rumen. In The rumen microbial ecosystem, second edition (ed. PN Hobson and CS Stewart), pp. 382–426. Blackie Academic & Professional, New York, NY, USA.CrossRefGoogle Scholar
Horrocks, LA 1972. Content, composition, and metabolism of mammalian and avian lipids that contain ether groups. In Ether lipids. Chemistry and biology (ed. F Snyder), pp. 177–272. Academic Press, New York, NY, USA.CrossRefGoogle Scholar
Juárez, M, Dugan, MER, Aalhus, JL, Aldai, N, Basarab, JA, Baron, VS, McAllister, TA 2010. Dietary vitamin E inhibits the trans10-18:1 shift in beef backfat. Canadian Journal of Animal Science 90, 9–12.CrossRefGoogle Scholar
Kraft, J, Collomb, M, Möckel, P, Sieber, R, Jahreis, G 2003. Differences in CLA isomer distribution of cow's milk lipids. Lipids 38, 657–664.CrossRefGoogle ScholarPubMed
Kraft, J, Kramer, JKG, Schoene, F, Chambers, JR, Jahreis, G 2008. Extensive analysis of long-chain polyunsaturated fatty acids, CLA, trans-18: 1 isomers, and plasmalogenic lipids in different retail beef types. Journal of Agricultural Food Chemistry 56, 4775–4782.CrossRefGoogle ScholarPubMed
Kramer, JKG, Hernandez, M, Cruz-Hernandez, C, Kraft, J, Dugan, MER 2008. Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demonstrated using ag-ion SPE fractionation. Lipids 43, 259–273.CrossRefGoogle Scholar
Kramer, JKG, Fellner, V, Dugan, MER, Sauer, FD, Mossoba, MM, Yurawecz, MP 1997. Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32, 1219–1228.CrossRefGoogle ScholarPubMed
Kramer, JKG, Sehat, N, Dugan, MER, Mossoba, MM, Yurawecz, MP, Roach, JAG, Eulitz, K, Aalhus, JL, Schaefer, AL, Ku, Y 1998. Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography. Lipids 33, 549–558.CrossRefGoogle ScholarPubMed
Leheska, JM, Thompson, LD, Howe, JC, Hentges, E, Boyce, J, Brooks, JC, Shriver, B, Hoover, L, Miller, MF 2008. Effects of conventional and grass feeding systems on the nutrient composition of beef. Journal of Animal Science 86, 3575–3585.CrossRefGoogle ScholarPubMed
Lough, AK 1977. The phytanic acid content of the lipids of bovine tissues and milk. Lipids 12, 115–119.CrossRefGoogle ScholarPubMed
Martínez, A, Aldai, N, Celaya, R, Osoro, K 2010. Effect of breed body size and the muscular hypertrophy gene in the production and carcass traits of concentrate-finished yearling bulls. Journal of Animal Science 88, 1229–1239.CrossRefGoogle ScholarPubMed
Mensink, RP, Zock, PL, Kester, ADM, Katan, MB 2003. Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials. American Journal of Clinical Nutrition 77, 1146–1155.CrossRefGoogle ScholarPubMed
Nuernberg, K, Nuernberg, G, Ender, K, Lorenz, S, Winkler, K, Rickert, R, Steinhart, H 2002. N-3 fatty acids and conjugated linoleic acids of longissimus muscle in beef cattle. European Journal of Lipid Science and Technology 104, 463–471.3.0.CO;2-U>CrossRefGoogle Scholar
Nuernberg, K, Dannenberger, D, Nuernberg, G, Ender, K, Voigt, J, Scollan, ND, Wood, JD, Nute, GR, Richardson, RI 2005. Effect of a grass-based and a concentrate feeding system on meat quality characteristics and fatty acid composition of longissimus muscle in different cattle breeds. Livestock Production Science 94, 137–147.CrossRefGoogle Scholar
Odegaard, A, Pereira, MA 2006. Trans fatty acids, insulin resistance, and type 2 diabetes. Nutrition Reviews 64, 364–372.CrossRefGoogle ScholarPubMed
Patton, S, Benson, AA 1966. Phytol metabolism in the bovine. Biochimica et Biophysica Acta 125, 22–32.CrossRefGoogle Scholar
Pérez, A 2005. Meat production in Asturias: beef cattle and other species (2003–2004). Statistical Report of the Rural and Marine Council of the Principality of Asturias, Spain.Google Scholar
Perez, R, Cañón, J, Dunner, S 2010. Genes associated with long-chain omega-3 fatty acids in bovine skeletal muscle. Journal of Applied Genetics 51, 479–487.CrossRefGoogle ScholarPubMed
Ponnampalam, EN, Mann, NJ, Sinclair, AJ 2006. Effect of feeding systems on omega-3 fatty acids, conjugated linoleic acid and trans fatty acids in Australian beef cuts: potential impact on human health. Asia Pacific Journal of Clinical Nutrition 15, 21–29.Google ScholarPubMed
Post-Beittenmiller, D 1996. Biochemistry and molecular biology of wax production in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47, 405–430.CrossRefGoogle ScholarPubMed
Pottier, J, Focant, M, Debier, C, De Buysser, G, Goffe, C, Mignolet, E, Froidmont, E, Larondelle, Y 2006. Effect of dietary vitamin E on rumen biohydrogenation pathways and milk fat depression in dairy cows fed high-fat diets. Journal of Dairy Science 89, 685–692.CrossRefGoogle ScholarPubMed
Purchas, RW, Knight, TW, Busboom, JR 2005. The effect of production system and age on concentrations of fatty acids in intramuscular fat of the longissimus and triceps brachii muscles of Angus-cross heifers. Meat Science 70, 597–603.CrossRefGoogle ScholarPubMed
Raes, K, De Smet, S, Balcaen, A, Claeys, E, Demeyer, D 2003. Effect of diets rich in N-3 polyunsatured fatty acids on muscle lipids and fatty acids in Belgian Blue double-muscled young bulls. Reproduction Nutrition Development 43, 331–345.CrossRefGoogle Scholar
Ratnayake, WMN, Zehaluk, C 2005. Trans fatty acids in foods and their labeling regulations. In Healthful lipids (ed. CC Akoh and OM Lai), pp. 1–32. AOCS Press, Champaign, IL, USA.Google Scholar
Rego, OA, Alves, SP, Antunes, LMS, Rosa, HJD, Alfaia, CFM, Prates, JAM, Cabrita, ARJ, Fonseca, AJM, Bessa, RJB 2009. Rumen biohydrogenation-derived fatty acids in milk fat from grazing dairy cows supplemented with rapeseed, sunflower, or linseed oils. Journal of Dairy Science 92, 4530–4540.CrossRefGoogle ScholarPubMed
Roy, A, Chardigny, JM, Bauchart, D, Ferlay, A, Lorenz, S, Durand, D, Gruffat, D, Faulconnier, Y, Sébédio, JL, Chilliard, Y 2007. Butter rich in trans10-C18:1 plus cis9, trans11-CLA differentially affects plasma lipids and aortic streak in experimental atherosclerosis in rabbits. Animal 1, 467–476.CrossRefGoogle Scholar
Rule, DC, Smith, SB, Romans, JR 1995. Fatty acid composition of muscle and adipose tissue of meat animals. In Biology of fat in meat animals (ed. SB Smith and DR Smith), pp. 144–165. American Society of Animal Science, Champaign, IL, USA.Google Scholar
SAS Institute 2001. SAS user's guide: statistics. SAS for Windows, release 9.2. SAS Institute Inc., Cary, NC, USA.Google Scholar
Sukhija, PS, Palmquist, DL 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36, 1202–1206.CrossRefGoogle Scholar
Vanhatalo, A, Kuoppala, K, Toivonen, V, Shingfield, KJ 2007. Effects of forage species and stage of maturity on bovine milk fatty acid composition. European Journal of Lipid Science and Technology 109, 856–867.CrossRefGoogle Scholar
Wolff, RL 2002. Characterization of trans-monounsaturated alkenyl chains in total plasmalogens (1-O-alk-1’-enyl-2-acyl glycerophospholipids) from sheep heart. Lipids 37, 811–816.CrossRefGoogle Scholar
Wood, JD, Enser, M, Fisher, AV, Nute, GR, Sheard, PR, Richardson, RI, Hughes, SI, Whittington, FM 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.CrossRefGoogle ScholarPubMed
Xu, J, Thornburg, T, Turner, AR, Vitolins, M, Case, D, Shadle, J, Hinson, L, Sun, J, Liu, W, Chang, B, Adams, TS, Zheng, SL, Torti, FM 2005. Serum levels of phytanic acid are associated with prostate cancer risk. The Prostate 63, 209–214.CrossRefGoogle ScholarPubMed