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Lean and obese pig breeds exhibit differences in prenatal gene expression profiles of muscle development

Published online by Cambridge University Press:  17 September 2014

X. R. Yang ,
B. Yu ,
X. B. Mao ,
P. Zheng ,
J. He ,
J. Yu ,
Y. He ,
J. M. Reecy  and
D. W. Chen
X. R. Yang
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
B. Yu
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
X. B. Mao
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
P. Zheng
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
J. He
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
J. Yu
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
Y. He
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
J. M. Reecy
Affiliation:
Department of Animal Science, Iowa State University, Ames, IA 50010, USA
D. W. Chen*
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
*
†E-mail: [email protected]
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Abstract

Muscle development in domesticated animals is important for meat production. Furthermore, intramuscular fat content is an important trait of meat intended for consumption. Here, we examined differences in the expression of factors related to myogenesis, adipogenesis and skeletal muscle growth during fetal muscle development of lean (Yorkshire) and obese (Chenghua) pig breeds. At prenatal days 50 (d50) and 90 (d90), muscles and sera were collected from pig fetuses. Histology revealed larger diameters and numbers of myofibers in Chenghua pig fetuses than those in Yorkshire pig fetuses at d50 and d90. Yorkshire fetuses had higher serum concentrations of myostatin (d90), a negative regulator for muscle development, and higher mRNA expression of the growth hormone receptor Ghr (d90), myogenic MyoG (d90) and adipogenic LPL (d50). By contrast, Chenghua fetuses exhibited higher serum concentration of growth hormone (d90), and higher mRNA expression of myogenic MyoD (d90) as well as adipogenic PPARG and FABP4 (d50). Our results revealed distinct expression patterns in the two pig breeds at each developmental stage before birth. Compared with Chenghua pigs, development and maturation of fetal skeletal muscles may occur earlier in Yorkshire pigs, but the negative regulatory effects of myostatin may suppress muscle development at the later stage.

Keywords

breed differencepigfetal muscle developmentmyostatinmyogenesis
Type
Research Article
Information
animal , Volume 9 , Supplement 1 , January 2015 , pp. 28 - 34
Copyright
© The Animal Consortium 2014 

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