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In vivo immunomodulatory effects of plant flavonoids in lipopolysaccharide-challenged broilers

Published online by Cambridge University Press:  15 April 2016

A. A. Kamboh ,
S.-Q. Hang ,
M. A. Khan  and
W.-Y. Zhu
A. A. Kamboh
Affiliation:
Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
S.-Q. Hang
Affiliation:
Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
M. A. Khan
Affiliation:
Department of Food Science and Technology, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
W.-Y. Zhu*
Affiliation:
Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
*
†E-mail: [email protected]
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Abstract

Plant flavonoids are generally regarded as natural replacers of synthetic growth promoters in poultry production. This study investigated the immunomodulatory effects of plant flavonoids, such as genistein and hesperidin, in lipopolysaccharide (LPS)-challenged broilers. A total of 700 21-day-old commercial Arbor Acres broiler chicks were randomly assigned into six treatment groups, each having six pens of 20 chicks/pen. Chicks were fed a basal diet without any additive (control, CON), 5 mg genistein/kg feed (G5), 20 mg hesperidin/kg (H20), or a basal diet with a combination of genistein and hesperidin (1 : 4) with doses of 5 mg/kg feed (GH5), 10 mg/kg (GH10) and 20 mg/kg (GH20) for 6 weeks. Half of the birds from each treatment were separated, and either challenged with 0·9% sodium chloride solution or Escherichia coli LPS (250 μg/kg BW) on days 16, 18 and 20. The results showed that both genistein and hesperidin improved (P<0.01) the plasma antioxidant status of growing broilers, by increasing total antioxidant capacity (TAOC), superoxide dismutase (SOD) activity and decreasing malondialdehyde production. LPS challenge further increased (P<0.05) TAOC and SOD levels. Regardless of LPS challenge, both genistein and hesperidin improved the humoral and mucosal immunity by increasing the intestinal intraepithelial lymphocyte numbers (P<0.01), as well as anti-Newcastle disease and anti-avian influenza antibody titers (P<0.05). Supplementation of both the plant flavonoids generally increased (P<0.05) the immune organs indices (spleen, thymus and bursa). Thus, supplementation of basal diet of broiler chicks, either with genistein or hesperidin, improved immune and antioxidant status of growing broilers. In addition, combined supplementation of both the flavonoids showed further improvement than individual compounds.

Keywords

genisteinhesperidinimmunomodulationLPS challengeantioxidant status
Type
Research Article
Information
animal , Volume 10 , Issue 10 , October 2016 , pp. 1619 - 1625
Copyright
© The Animal Consortium 2016 

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References

Ali, AH, Abdul-Azeez, LA, Humood, JK, Ali, ZA, Helal, ZH and Wahab, FL 2016. The effect of ethanolic extract of Hibiscus sabdariffa on some physiological and antioxidant parameters in female rabbits. Journal of Animal Health and Production 4, 37–41.Google Scholar
Ansari, ARMIH, Rahman, MM, Islam, MZ, Das, BC, Habib, A, Belal, SMSH and Islam, K 2014. Prevalence and antimicrobial resistance profile of Escherichia coli and salmonella isolated from diarrheic calves. Journal of Animal Health and Production 2, 12–15.Google Scholar
Bowen, OT, Dienglewicz, RL, Wideman, RF and Erf, GF 2009. Altered monocyte and macrophage numbers in blood and organs of chickens injected i.v. with lipopolysaccharide. Veterinary Immunology and Immunopathology 131, 200–210.CrossRefGoogle ScholarPubMed
Calixto, JB, Campos, MM, Otuki, MF and Santos, AR 2004. Anti-inflammatory compounds of plant origin. Part II. Modulation of pro-inflammatory cytokines, chemokines and adhesion molecules. Planta Medica 70, 93–103.Google Scholar
Catoni, C, Peters, A and Schaefer, M 2008. Life-history trade-offs are influenced by the diversity, availability and interactions of dietary antioxidants. Animal Behaviour 76, 1107–1119.CrossRefGoogle Scholar
Chavan, UD, Amarowicz, R and Shahidi, F 1999. Antioxidant activity of phenolic fractions of beach pea (Lathyrus maritimus L.). Journal of Food Lipids 6, 1–11.Google Scholar
Cox, CM, Stuard, LH, Kim, S, McElroy, AP, Bedford, MR and Dalloul, RA 2010. Performance and immune responses to dietary beta-glucan in broiler chicks. Poultry Science 89, 1924–1933.Google Scholar
Dong, XF, Gao, WW, Tong, JM, Jia, HQ, Sa, RN and Zhang, Q 2007. Effect of polysavone (alfalfa extract) on abdominal fat deposition and immunity in broiler chickens. Poultry Science 86, 1955–1959.CrossRefGoogle ScholarPubMed
Fellenberg, MA and Speisky, H 2006. Antioxidants: their effects on broiler oxidative stress and its meat oxidative stability. World Poultry Science Journal 62, 53–70.CrossRefGoogle Scholar
Goliomytis, M, Kartsonas, N, Charismiadou, MA, Symeon, GK, Simitzis, PE and Deligeorgis, SG 2015. The influence of naringin or hesperidin dietary supplementation on broiler meat quality and oxidative stability. PLoS One 10, e0141652.Google Scholar
Goliomytis, M, Tsoureki, D, Simitzis, PE, Charismiadou, MA, Hager-Theodorides, AL and Deligeorgis, SG 2014. The effects of quercetin dietary supplementation on broiler growth performance, meat quality, and oxidative stability. Poultry Science 93, 1957–1962.Google Scholar
Guo, TL Jr, White, KL, Brown, RD, Delclos, KB, Newbold, RR, Weis, C, Germolec, DR and McCay, JA 2002. Genistein modulates splenic natural killer cell activity, antibody-forming cell response, and phenotypic marker expression in F(0) and F(1) generations of Sprague-Dawley rats. Toxicology and Applied Pharmacology 181, 219–227.CrossRefGoogle Scholar
Hager-Theodorides, AL, Goliomytis, M, Delis, S and Deligeorgis, S 2014. Effects of dietary supplementation with quercetin on broiler immunological characteristics. Animal Feed Science and Technology 198, 224–230.Google Scholar
Hanieh, H, Gerile, C, Narabara, K, Gu, Z, Abe, A and Kondo, Y 2010. In vivo immunomodulatory effects of dietary purple sweet potato after immunization in chicken. Animal Science Journal 81, 116–121.CrossRefGoogle ScholarPubMed
Inagaki-Ohara, K, Sawaguchi, A, Suganuma, T, Matsuzaki, G and Nawa, Y 2005. Intraepithelial lymphocytes express junctional molecules in murine small intestine. Biochemical and Biophysical Research Communications 331, 977–983.Google Scholar
Jiang, SQ, Jiang, ZY, Zhou, GI, Lin, YC and Zheng, CT 2014. Effects of dietary isoflavone supplementation on meat quality and oxidative stability during storage in Lingnan yellow broilers. Journal of Integrative Agriculture 13, 387–393.Google Scholar
Jiang, ZY, Jiang, SQ, Lin, YC, Xi, PB, Yu, DQ and Wu, TX 2007. Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers. Poultry Science 86, 1356–1362.Google Scholar
Kamboh, AA, Arain, MA, Mughal, MJ, Zaman, A, Arain, ZM and Soomro, AH 2015. Flavonoids: health promoting phytochemicals for animal production – a review. Journal of Animal Health and Production 3, 6–13.Google Scholar
Kamboh, AA, Hang, SQ, Bakhetgul, M and Zhu, WY 2013. Effects of genistein and hesperidin on biomarkers of heat stress in broilers under persistent summer stress. Poultry Science 92, 2404–2410.Google Scholar
Kamboh, AA and Zhu, WY 2013. Effect of increasing levels of bioflavonoids in broiler feed on plasma anti-oxidative potential, lipid metabolites, and fatty acid composition of meat. Poultry Science 92, 454–461.Google Scholar
Kamboh, AA and Zhu, WY 2014. Individual and combined effects of genistein and hesperidin on immunity and intestinal morphometry in lipopolysaccharide-challenged broiler chickens. Poultry Science 93, 1–9.CrossRefGoogle Scholar
Kawaguchi, K, Kikuchi, S, Hasunuma, R, Maruyama, H, Yoshikawa, T and Kumazawa, Y 2004. A citrus flavonoid hesperidin suppresses infection-induced endotoxin shock in mice. Biological and Pharmaceutical Bulletin 27, 679–683.Google Scholar
Lien, TF, Yeh, HS and Su, WT 2008. Effect of adding extracted hesperetin, naringenin and pectin on egg cholesterol, serum traits and antioxidant activity in laying hens. Archives of Animal Nutrition 62, 33–43.Google Scholar
Liu, T, She, R, Wang, K, Bao, H, Zhang, Y, Luo, D, Hu, Y, Ding, Y, Wang, D and Peng, K 2008. Effects of rabbit sacculus rotundus antimicrobial peptides on the intestinal mucosal immunity in chickens. Poultry Science 87, 250–254.Google Scholar
Mailk, S, Kumar, A, Verma, AK, Gupta, MK, Sharma, SD, Sharma, AK and Rahal, A 2013. Incidence and drug resistance pattern of collibacillosis in cattle and buffalo calves in western Uttar Pradesh in India. Journal of Animal Health and Production 1, 15–19.Google Scholar
Marzoni, M, Chiarini, R, Castillo, A, Romboli, I, De Marco, M and Schiavone, A 2014. Effects of dietary natural antioxidant supplementation on broiler chicken and Muscovy duck meat quality. Animal Science Papers Reports 32, 359–368.Google Scholar
National Research Council 1994. Nutrient requirements of poultry, 9th revised edition. National Academy Press, Washington, DC, USA.Google Scholar
Nghonjuyi, NW, Tiambo, CK, Kimbi, HK, Manka’a, CN, Juliano, RS and Lisita, F 2015. Efficacy of ethanolic extract of Carica papaya leaves as a substitute of sulphanomide for the control of coccidiosis in kabir chickens in Cameroon. Journal of Animal Health and Production 3, 21–27.Google Scholar
Ogikubo, Y, Orimatsu, M, Sasaki, Y, Yasuda, A, Saegusa, J and Tamura, Y 2004. Effect of lipopolysaccharide (LPS) injection on the immune responses of LPS-sensitive mice. Journal of Veterinary Medicine Science 66, 1189–1193.Google Scholar
Raheema, RH 2016. Effect of pomegranate peel extract on some biochemical and histopathological parameters in experimental induced mice with Staphylococcus aureus . Journal of Animal Health and Production 4, 42–49.Google Scholar
Rahman, S, Nizamani, ZA, Soomro, NM, Kalhoro, NH and Rasool, F 2014. Velogenic viscerotropic Newcastle disease virus produces variable pathogenicity in two chicken breeds. Journal of Animal Health and Production 2, 46–50.Google Scholar
Shukla, S, Mehta, A, John, J, Mehta, P, Vyas, SP and Shukla, S 2009. Immunomodulatory activities of the ethanolic extract of Caesalpinia bonducella seeds. Journal of Ethnopharmacology 125, 252–256.CrossRefGoogle ScholarPubMed
Simitzis, PE, Ilias-Dimopoulos, V, Charismiadou, MA, Biniari, EE and Deligeorgis, SG 2013. Effects of dietary hesperidin supplementation on lamb performance and meat characteristics. Animal Science Journal 84, 136–143.Google Scholar
Simitzis, PE, Symeon, GK, Charismiadou, MA, Ayoutanti, AG and Deligeorgis, SG 2011. The effects of dietary hesperidin supplementation on broiler performance and chicken meat characteristics. Canadian Journal of Animal Science 91, 275–282.CrossRefGoogle Scholar
SPSS 1999. SPSS version 10.0 for Windows. SPSS Inc., Chicago, IL, USA.Google Scholar
Wei, J, Bhatt, S, Chang, LM, Sampson, HA and Masilamani, M 2012. Isoflavones, genistein and daidzein, regulate mucosal immune response by suppressing dendritic cell function. PLoS One 7, e47979.Google Scholar
Zhang, X, Zhong, X, Zhou, Y, Wang, G, Du, H and Wang, T 2010. Dietary RRR-a-tocopherol succinate attenuates lipopolysaccharide-induced inflammatory cytokines secretion in broiler chicks. British Journal of Nutrition 104, 1796–1805.CrossRefGoogle Scholar