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Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel

Published online by Cambridge University Press:  23 January 2020

M. Morini ,
A. G. Lafont ,
G. Maugars ,
S. Baloche ,
S. Dufour ,
J. F. Asturiano Open the ORCID record for J. F. Asturiano [Opens in a new window]  and
L. Pérez
M. Morini
Affiliation:
Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera s/n, 46022Valencia, Spain
A. G. Lafont
Affiliation:
Muséum National d’Histoire Naturelle, Sorbonne Universités, Research Unit BOREA, Biology of Aquatic Organisms and Ecosystems, CNRS, IRD, SU, UCN, UA, 7 rue Cuvier 75231Paris 05, France
G. Maugars
Affiliation:
Muséum National d’Histoire Naturelle, Sorbonne Universités, Research Unit BOREA, Biology of Aquatic Organisms and Ecosystems, CNRS, IRD, SU, UCN, UA, 7 rue Cuvier 75231Paris 05, France
S. Baloche
Affiliation:
Muséum National d’Histoire Naturelle, Sorbonne Universités, Research Unit BOREA, Biology of Aquatic Organisms and Ecosystems, CNRS, IRD, SU, UCN, UA, 7 rue Cuvier 75231Paris 05, France
S. Dufour
Affiliation:
Muséum National d’Histoire Naturelle, Sorbonne Universités, Research Unit BOREA, Biology of Aquatic Organisms and Ecosystems, CNRS, IRD, SU, UCN, UA, 7 rue Cuvier 75231Paris 05, France
J. F. Asturiano*
Affiliation:
Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera s/n, 46022Valencia, Spain
L. Pérez
Affiliation:
Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera s/n, 46022Valencia, Spain
*
†E-mail: [email protected]
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Abstract

In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel.

Keywords

Anguilla anguillateleostlipoprotein receptor 8oogenesisreproduction
Type
Research Article
Information
animal , Volume 14 , Issue 6 , June 2020 , pp. 1213 - 1222
Copyright
© The Animal Consortium 2020

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