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Grape pomace improves performance, antioxidant status, fecal microbiota and meat quality of piglets

Published online by Cambridge University Press:  17 July 2017

I. Kafantaris
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece Animal Research Institute/Hellenic Agricultural Organization (HAO) – Demeter, 58100 Giannitsa, Greece
D. Stagos
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
B. Kotsampasi
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
A. Hatzis
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
A. Kypriotakis
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
K. Gerasopoulos
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
S. Makri
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
N. Goutzourelas
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
C. Mitsagga
Affiliation:
Lab of Food Microbiology and Biotechnology, Department of Food Technology, Technical Education Institute of Thessaly, 43100 Karditsa, Greece
I. Giavasis
Affiliation:
Lab of Food Microbiology and Biotechnology, Department of Food Technology, Technical Education Institute of Thessaly, 43100 Karditsa, Greece
K. Petrotos
Affiliation:
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece
S. Kokkas
Affiliation:
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece
P. Goulas
Affiliation:
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece
V. Christodoulou
Affiliation:
Animal Research Institute/Hellenic Agricultural Organization (HAO) – Demeter, 58100 Giannitsa, Greece
D. Kouretas*
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
*
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Abstract

In the present study, grape pomace (GP) was used as feed additive in the diet of weaned piglets in order to develop innovative feedstuffs and to investigate their potential beneficial effects on welfare, productivity and meat quality. For examining the antioxidant capacity of the experimental feeds, 24 piglets of 20 days old were assigned to two experimental groups receiving standard or experimental diet for 30 days. Blood and tissues collections were performed at four different time-points, 2, 20, 35 and 50 days post birth. The collected tissues were brain, heart, kidney, liver, lung, quadriceps muscle, pancreas, spleen and stomach. The following oxidative stress markers were assessed: reduced glutathione (GSH), catalase activity, total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), protein carbonyls (CARB) and H2O2 decomposition activity. The effect on bacterial growth was assessed by examining microbial populations in piglets’ fecal microbiota. Furthermore, the average daily gain (ADG) was calculated and the fatty acid profile of quadriceps muscle was assessed. The results showed that piglets fed with the diet supplemented with GP, had significantly increased antioxidants mechanisms in almost all the tissues as shown by increases in GSH, H2O2 decomposition activity and TAC compared with control group. Piglets fed with the experimental diet exhibited decreased oxidative stress-induced damage to lipids and proteins as shown by decreases in TBARS and CARB in GP group compared with control. In addition, the experimental diet increased significantly ADG (by 23.65%) (P<0.05) and enhanced the growth of facultative probiotic bacteria (by up to 1.2 log colony forming units (CFU)/g) (P<0.05) and lactic acid bacteria (by up to 2.0 log CFU/g) (P<0.05) in GP group compared with the control group. GP supplementation inhibited the growth of pathogen populations such as Enterobacteriacae (by up to 1.8 log CFU/g) (P<0.05) and Campylobacter jejuni (by up to 1.0 log CFU/g) (P<0.05). Regarding fatty acid composition of meat, GP inclusion in piglets’ diet increased significantly n-3 fatty acids (EPA; C20 : 5n-3, DHA; C22 : 6n-3, α-linolenic acid; C18 : 3n-3) and decreased significantly n-6/n-3 ratio compared with control (P<0.05). The results suggested that dietary GP supplementation may have a beneficial impact on piglets’ welfare and may improve productivity as well as meat quality.

Type
Research Article
Copyright
© The Animal Consortium 2017 

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References

Agte, V, Khetmalis, N, Nilegaonkar, S, Karkamkar, S and Yadav, S 2010. Prebiotic potential of juice grape varieties and some hybrids. Journal of Scientific and Industrial Research 69, 850854.Google Scholar
Association of Official Analytical Chemists (AOAC) 1990. Official methods of analysis, volume 1, 15th edition. AOAC, Arlington, VA, USA.Google Scholar
Aquilano, K, Baldelli, S and Ciriolo, MR 2014. Glutathione: new roles in redox signaling for an old antioxidant. Frontiers in Pharmacology 26, 112.Google Scholar
Brewer, MT, Xiong, N, Anderson, KL and Carlson, SA 2013. Effects of subtherapeutic concentrations of antimicrobials on gene acquisition events in Yersinia, Proteus, Shigella, and Salmonella recipient organisms in isolated ligated intestinal loops of swine. American Journal of Veterinary Research 74, 10781083.Google Scholar
Calder, PC 2010. Omega-3 fatty acids and inflammatory processes. Nutrients 2, 355374.CrossRefGoogle ScholarPubMed
DeLeve, LD and Kaplowitz, N 1990. Importance and regulation of hepatic glutathione. Seminars in Liver Disease 10, 251266.CrossRefGoogle ScholarPubMed
Fiesel, A, Gessner, DK, Most, E and Eder, K 2014. Effects of dietary polyphenol-rich plant products from grape or hop on pro-inflammatory gene expression in the intestine, nutrient digestibility and faecal microbiota of weaned pigs. BMC Veterinary Research 10, 196206.CrossRefGoogle ScholarPubMed
Gerasopoulos, K, Stagos, D, Kokkas, S, Petrotos, K, Kantas, D, Goulas, P and Kouretas, D 2015a. Feed supplemented with byproducts from olive oil mill wastewater processing increases antioxidant capacity in broiler chickens. Food and Chemical Toxicology 82, 4249.Google Scholar
Gerasopoulos, K, Stagos, D, Krouezas, A, Karaveli, C, Barda, C, Gkika, H, Mitsiou, D, Petrotos, K, Goulas, P and Kouretas, D 2016. Assessment of fatty acid allocation in plasma and tissues in piglets, using feed supplemented with byproducts from processed olive mill wastewater. In Vivo 30, 291301.Google ScholarPubMed
Gerasopoulos, K, Stagos, D, Petrotos, K, Kokkas, S, Kantas, D, Goulas, P and Kouretas, D 2015b. Feed supplemented with polyphenolic byproduct from olive mill wastewater processing improves the redox status in blood and tissues of piglets. Food and Chemical Toxicology 86, 319327.CrossRefGoogle ScholarPubMed
Goñi, I, Brenes, A, Centeno, C, Viveros, A, Saura-Calixto, F, Rebole, A, Arija, I and Estevez, R 2007. Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility and susceptibility to meat lipid oxidation in chickens. Poultry Science 86, 508516.Google Scholar
Goutzourelas, N, Stagos, D, Demertzis, N, Mavridou, P, Karterolioti, H, Georgadakis, S, Kerasioti, E, Aligiannis, N, Skaltsounis, L, Statiri, A, Tsioutsiouliti, A, Tsatsakis, AM, Hayes, AW and Kouretas, D 2014. Effects of polyphenolic grape extract on the oxidative status of muscle and endothelial cells. Human and Experimental Toxicology 33, 10991112.CrossRefGoogle ScholarPubMed
Goutzourelas, N, Stagos, D, Housmekeridou, A, Karapouliou, C, Kerasioti, E, Aligiannis, N, Skaltsounis, AL, Spandidos, DA, Tsatsakis, AM and Kouretas, D 2015a. Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells. International Journal of Molecular Medicine 36, 433444.Google Scholar
Goutzourelas, N, Stagos, D, Spanidis, Y, Liosi, M, Apostolou, A, Priftis, A, Haroutounian, S, Spandidos, DA, Tsatsakis, AM and Kouretas, D 2015b. Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells. Molecular Medicine Reports 12, 58465856.Google Scholar
Habeanu, M, Lefter, NA, Ropota, M, Chedea, VS, Gheorghe, A, Toma, SM, Ciurescu, G and Dragomir, C 2015. Dried grape pomace influenced fatty acids composition of Longissimus dorsi muscle and plasma polyphenols spectrum in finishing pigs. The Indian Journal of Animal Sciences 85, 786789.Google Scholar
Kafantaris, I, Kotsampasi, B, Christodoulou, V, Kokka, E, Kouka, P, Terzopoulou, Z, Gerasopoulos, K, Stagos, D, Mitsagga, C, Giavasis, I, Makri, S, Petrotos, K and Kouretas, D 2016. Grape pomace improves antioxidant capacity and faecal microflora of lambs. Journal of Animal Physiology and Animal Nutrition, https://doi.org/10.1111/jpn.12569.Google ScholarPubMed
Lauritzen, L, Hensen, HS, Jorgensen, MH and Michaelsen, KF 2001. The essentiality of long chain n-3 fatty acids in relation to development and function of the brain and retina. Progress in Lipid Research 40, 194.CrossRefGoogle ScholarPubMed
Lykkesfeldt, J and Svendsen, O 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. The Veterinary Journal 173, 502511.Google Scholar
Makri, S, Kafantaris, I, Stagos, D, Chamokeridou, T, Petrotos, K, Gerasopoulos, K, Mpesios, A, Goutzourelas, N, Kokkas, S, Goulas, P, Komiotis, D and Kouretas, D 2017. Novel feed including bioactive compounds from winery wastes improved broilers’ redox status in blood and tissues of vital organs. Food and Chemical Toxicology 102, 2431.CrossRefGoogle ScholarPubMed
Montagne, L, Pluske, JR and Hampson, DJ 2003. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology 108, 95117.Google Scholar
National Research Council 1996. Guide for the care and use of laboratory animals. National Academy Press, Washington, DC, USA.Google Scholar
National Research Council (NRC) 2012. Nutrient requirements of swine, 11th revised edition. National Academic Press, Washington, DC, USA.Google Scholar
Noblet, J and Perez, JM 1993. Prediction of digestibility of nutrients and energy values of pig diets from chemical analysis. Journal of Animal Science 71, 33893398.Google Scholar
Núñez, Y, Fernández, A, Benítez, R, Arija, I, Viveros, A, Brenes, A and Ovilo, C 2016. Dietary supplementation with vitamin E or grape pomace influences antioxidant and lipid metabolism candidate gene expression in broiler muscle. Journal of Animal Science 94, 6363.Google Scholar
Parry, JW, Li, H, Liu, JR, Zhou, K, Zhang, L and Ren, S 2011. Antioxidant activity, antiproliferation of colon cancer cells, and chemical composition of grape pomace. Food and Nutrition Sciences 2, 530540.CrossRefGoogle Scholar
Payne, RL and Southern, LL 2005. Changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium. Poultry Science 84, 12681276.Google Scholar
Rapoport, SI, Rao, JS and Igarashi, M 2007. Brain metabolism of nutritionally essential poly-unsaturated fatty acids depends on both the diet and the liver. Prostaglandins, Leukotrienes and Essential Fatty Acids 77, 251261.CrossRefGoogle Scholar
Ross, GR, Nieuwenhove, CP and González, SN 2012. Fatty acid profile of pig meat after probiotic administration. Journal of Agricultural and Food Chemistry 60, 59745978.Google Scholar
Schaffer, TK, Wohlenberg, MF, Medeiros, N, Martins, JB, Agostini, F, Funchal, C and Dani, C 2016. Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats. Anais da Academia Brasileira de Ciências 88, 187196.Google Scholar
Simopoulos, AP 2009. Evolutionary aspects of the dietary omega-6: omega-3 fatty acid ratio: medical implications. World Review of Nutrition and Dietetics Home 100, 121.Google Scholar
Viveros, A, Chamorro, S, Pizarro, M, Arija, I, Centeno, C and Brenes, A 2011. Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poultry Science 90, 566578.CrossRefGoogle ScholarPubMed
Wu, L, Zhang, Y, Ma, X, Zhang, N and Qin, G 2012. The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats. Molecular Biology Reports 39, 90859093.Google Scholar
Yan, L and Kim, IH 2011. Effect of dietary grape pomace fermented by Saccharomyces boulardii on the growth performance, nutrient digestibility and meat quality in finishing pigs. Asian-Australasian Journal of Animal Sciences 24, 17631770.CrossRefGoogle Scholar