Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-23T12:49:15.274Z Has data issue: false hasContentIssue false
  • English
  • Français

Flaxseed supplementation decreases methanogenic gene abundance in the rumen of dairy cows

Published online by Cambridge University Press:  12 April 2012

L. Li ,
K. E. Schoenhals ,
P. A. Brady ,
C. T. Estill ,
S. Perumbakkam  and
A. M. Craig
L. Li
Affiliation:
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis 97331, USA
K. E. Schoenhals
Affiliation:
College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA
P. A. Brady
Affiliation:
College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA
C. T. Estill
Affiliation:
College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA
S. Perumbakkam
Affiliation:
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis 97331, USA
A. M. Craig*
Affiliation:
College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA
*
†E-mail: [email protected]
Get access

Abstract

The objective of this study was to investigate the effects of a flaxseed-supplemented diet on archaeal abundance and gene expression of methanogens in the rumen of dairy cows. In all, 11 non-lactating dairy cows were randomly divided into two groups: group A (five cows) and B (six cows). The two diets fed were: (1) the control diet, a conventional dry cow ration; and (2) the flaxseed-supplemented diet, the conventional dry cow ration adjusted with 12.16% ground flaxseed incorporated into the total mixed ration. A cross-over experiment was performed with the two groups of cows fed the two different diets for five 21-day periods, which included the first adaptation period followed by two treatment and two wash out periods. At the end of each feeding period, rumen fluid samples were collected via rumenocentesis and DNA was extracted. Quantitative PCR was utilized to analyze the gene abundance of 16S ribosomal RNA (16S rRNA) targeting the ruminal archaea population and the mcrA gene coding for methyl coenzyme-M reductase subunit A, a terminal enzyme in the methanogenesis pathway. Results demonstrated a 49% reduction of 16S rRNA and 50% reduction of mcrA gene abundances in the rumen of dairy cows fed the flaxseed-supplemented diet in comparison with those fed the control diet. This shows flaxseed supplementation effectively decreases the methanogenic population in the rumen. Future studies will focus on the mechanisms for such reduction in the rumen of dairy cattle, as well as the relationship between methanogenic gene expression and methane production.

Keywords

flaxseedmethanogenic genemcra; dairy cattle
Type
Nutrition
Information
animal , Volume 6 , Issue 11 , November 2012 , pp. 1784 - 1787
Copyright
Copyright © The Animal Consortium 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beauchemin, KA, McGinn, SM, Benchaar, C, Holtshausen, L 2009. Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: effects on methane production, rumen fermentation, and milk production. Journal of Dairy Science 92, 2118–2127.Google Scholar
Case, RJ, Boucher, Y, Dahllöf, I, Holmström, C, Doolittle, WF, Kjelleberg, S 2007. Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. Applied and Environmental Microbiology 73, 278–288.Google Scholar
Denman, SE, Tomkins, NW, McSweeney, CS 2007. Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound romochloromethane. FEMS Microbiology Ecology 62, 313–322.Google Scholar
Eugène, M, Martina, C, Mialona, MM, Kraussb, D, Renandc, G, Doreau, M 2011. Dietary linseed and starch supplementation decreases methane production of fattening bulls. Animal Feed Science Technology 166–167, 330–337.Google Scholar
Fievez, V, Vlaeminck, B, Jenkins, T, Enjalbert, F, Doreau, M 2007. Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ. European Journal of Lipid Science and Technology 109, 740–756.Google Scholar
Garrett, EF, Pereira, MN, Nordlund, KV, Armentano, LE, Gooder, WJ, Oetzel, GR 1999. Diagnostic methods for the detection of subacute ruminal acidosis in dairy cows. Journal of Dairy Science 82, 1170–1178.Google Scholar
Janssen, PH, Kirs, M 2008. Structure of the archaeal community of the rumen. Applied and Environmental Microbiology 74, 3619–3625.Google Scholar
Luton, PE, Wayne, JM, Sharp, RJ, Riley, PW 2002. The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill. Microbiology 148, 3521–3530.Google Scholar
Martin, C, Morgavi, DP, Doreau, M 2010. Methane mitigation in ruminants: from microbe to the farm scale. Animal 4, 351–365.Google Scholar
Vianna, ME, Conrads, G, Gomes, BP, Horz, HP 2006. Identification and quantification of archaea involved in primary endodontic infections. Journal of Clinical Microbiology 44, 1274–1282.Google Scholar