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Faecal alkane recoveries from multi-component diets and effects on estimates of diet composition in sheep

Published online by Cambridge University Press:  01 January 2008

C. Elwert ,
H. Dove  and
M. Rodehutscord
C. Elwert*
Affiliation:
Institut für Agrar- und Ernährungswissenschaften, Martin-Luther-Universität Halle-Wittenberg, 06099 Halle, Germany
H. Dove
Affiliation:
CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia
M. Rodehutscord
Affiliation:
Institut für Agrar- und Ernährungswissenschaften, Martin-Luther-Universität Halle-Wittenberg, 06099 Halle, Germany
*
E-mail: [email protected]
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Abstract

In a feeding trial with sheep, four animals each were fed one of the three roughages (perennial ryegrass (PR), meadow fescue (MF) and red clover (RC)) or one of the four different mixtures (g/day 250 : 250 : 250, 375 : 75 : 300, 525 : 187.5 : 37.5 and 75 : 525 : 150, respectively) of those three roughages, both with and without the inclusion of 100 g of beeswax-labelled barley. Further, four sheep received a pure lucerne diet to enlarge available data on single species faecal recoveries. All sheep except those fed single-component diets and the 250 : 250 : 250 roughage mix were administered intra-ruminal alkane controlled-release devices (CRD). The aim of the study was to investigate the effect of diet composition on faecal alkane recoveries, and to assess the accuracy of the alkane-based estimate of diet composition and intake based either upon a general set of faecal recoveries across diets or upon dietary recoveries and dietary samples more specifically attributable to individual dietary treatments. For each roughage component independently, the accuracy of diet composition estimates was assessed using linear regression across all diets. The estimates of the proportion of barley were analysed further using mean differences and mean prediction errors. Faecal alkane recovery increased with increasing chain length and was affected by diet composition. RC had a significantly higher faecal recovery for alkanes C25, C27, C29 and C31 than at least one of the other single-roughage diets. When considering mixed diets consisting of PR, MF, RC and barley, the composition of the roughage component significantly affected faecal recovery of all alkanes except C30 and C33. The inclusion of beeswax-labelled barley caused a decrease in faecal recovery of alkanes up to C29. This effect was attributable to the beeswax rather than the barley itself. By contrast, the decrease of faecal recovery of synthetic dosed alkanes from the CRD in diets containing barley, compared with the corresponding diets without barley, was attributable to the supplement itself. It was concluded that synthetic dosed alkanes behaved differently during gut transit from natural alkanes. The proportions of individual dietary components were estimated well over a wide range of proportions. Generally, the more information available, the more accurate the estimates achieved. However, a general set of faecal alkane recoveries and bulked samples of dietary components yielded estimates of diet composition sufficiently accurate for a large number of studies, especially in situations where groups of animals are of concern and not the individual animal.

Keywords

alkanesdiet compositionfaecal recoveryroughagesupplement
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 125 - 134
Copyright
Copyright © The Animal Consortium 2008

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References

Brosh, A, Henkin, Z, Rothman, SJ, Aharoni, Y, Orlov, A, Arieli, A 2003. Effects of faecal n-alkane recovery in estimates of diet composition. Journal of Agricultural Science, Cambridge 140, 93–100.CrossRefGoogle Scholar
Charmley, E, Dove, H 2007. Using plant wax markers to estimate intakes of mixed forages in sheep by feeding a known amount of supplement: estimates based on eight n-alkanes. Australian Journal of Agricultural Research 58 (12), in press.CrossRefGoogle Scholar
Deutsche Landwirtschafts-Gesellschaft (DLG) 1997. DLG-Futterwerttabellen für wiederkäuer (feedstuff tables for ruminants), 7th edition. DLG-Verlag, Frankfurt am Main, Germany.Google Scholar
Dicker, RW, Herd, RM, Oddy, VH 1996. Alkanes and controlled release devices for estimating intake of ryegrass by cattle. Proceedings of the Nutrition Society of Australia 20, 107.Google Scholar
Dillon PG 1993. The use of n-alkanes as markers to determine herbage intake, botanical composition of available or consumed herbage and in studies of digesta kinetics with dairy cows. PhD thesis, National University of Ireland, UK.Google Scholar
Dillon P and Stakelum G 1988. The use of n-alkanes and chromic oxide as markers for determing feed intake, faecal output and digestibility in dairy cows. Proceedings of the 12th General meeting of the European Grassland Federation, Dublin, Ireland, pp. 154–158.Google Scholar
Dove, H, Mayes, RW 1991. The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42, 913–952.CrossRefGoogle Scholar
Dove, H, Moore, AD 1995. Using a least-squares optimization procedure to estimate botanical composition based on the alkanes of plant cuticular wax. Australian Journal of Agricultural Research 46, 1535–1544.CrossRefGoogle Scholar
Dove, H, Oliván, M 1998. Using synthetic or beeswax alkanes for estimating supplement intake in sheep. Animal Production in Australia 22, 189–192.Google Scholar
Dove H, Mayes RW, Freer M, Coombe JB and Foot JZ 1989. Faecal recoveries of the alkanes of plant cuticular waxes in penned and in grazing sheep. Proceedings of the 16th International Grassland Congress, Nice, France, pp. 1093–1094.Google Scholar
Dove, H, Mayes, RW, Lamb, CS, Ellis, KJ 2002a. Factors influencing the release rate of alkanes from an intra-ruminal, controlled-release device, and the resultant accuracy of intake estimation in sheep. Australian Journal of Agricultural Research 53, 681–696.CrossRefGoogle Scholar
Dove, H, Scharch, C, Oliván, M, Mayes, RW 2002b. Using n-alkanes and known supplement intake to estimate roughage intake in sheep. Animal Production in Australia 24, 57–60.Google Scholar
Dove, H, Charmley, E, Kleven, K 2003. Using n-alkanes to estimate intakes of mixed forages by feeding a known amount of an alkane-labelled supplement. Canadian Journal of Animal Science 83, 641–642.Google Scholar
Elwert C 2004. Studies on the use of alkanes to estimate diet composition, intake, and digestibility in sheep. PhD thesis, Martin Luther University Halle-Wittenberg, Germany.Google Scholar
Elwert, C, Dove, H 2005. Estimation of roughage intake using a known daily intake of a labelled supplement. Animal Science 81, 47–56.CrossRefGoogle Scholar
Elwert, C, Rodehutscord, M 2005a. Theoretical considerations on a one-parameter approach to compare actual and estimated compositions of multi-component diets. In Proceedings of the 20th International Grassland Congress (ed. FP O′Mara, RJ Wilkins, LDK Lovett, PAM Rogers and TM Boland), p. 496. Wageningen Academic Publishers, Wageningen, NL.Google Scholar
Elwert, C, Rodehutscord, M 2005b. Practical application of a one-parameter approach to assess the accuracy of two different estimates of diet composition in sheep. In Utilisation of grazed grass in temperate animal systems. Satellite workshop of the 20th International Grassland Congress (ed. JJ Murphy), p. 249. Wageningen Academic Publishers, Wageningen, NL.CrossRefGoogle Scholar
Elwert, C, Kluth, H, Rodehutscord, M 2004. Effect of variable intake of alfalfa and wheat on faecal alkane recoveries and estimates of roughage intake in sheep. Journal of Agricultural Science, Cambridge 142, 213–223.CrossRefGoogle Scholar
Elwert, C, Dove, H, Rodehutscord, M 2006. Effect of roughage species on fecal alkane recovery in sheep, and effect of sample drying treatment on alkane concentrations. Australian Journal of Experimental Agriculture 46, 771–776.CrossRefGoogle Scholar
Ferreira, LMM, Oliván, M, Garcia, U, Rodrigues, MAM, Osoro, K 2005. Validation of the alkane technique to estimate diet selection of goats grazing heather-gorse vegetation communities. Journal of the Science of Food and Agriculture 85, 1636–1646.CrossRefGoogle Scholar
Hendricksen, RE, Reich, MM, Roberton, RF, Reid, DJ, Gazzola, C, Rideout, JA, Hill, RA 2002. Estimating the voluntary intake and digestibility of buffel-grass and lucerne hays offered to Brahman-cross cattle using n-alkanes. Animal Science 74, 567–577.CrossRefGoogle Scholar
Herd, RM, Williams, TMJ, Woodgate, R, Ellis, KJ, Oddy, VH 1996. Using alkane technology to measure intake of a barley diet by cattle. Proceedings of the Nutrition Society of Australia 20, 106.Google Scholar
Jeffree, CE 1986. The cuticle, epicuticular waxes and trichomes of plants, with reference to their structure, functions and evolution. In Insects and the plant surface (ed. B Juniper and TRE Southwood), pp. 23–64. Edward Arnold, London, UK.Google Scholar
Jetter, R, Schaeffer, S, Riederer, M 2000. Leaf cuticular waxes are arranged in chemically and mechanically distinct layers: evidence from Prunus laurocerasus L. Plant Cell and Environment 23, 619–628.CrossRefGoogle Scholar
Lin, L-J, Luo, H-L, Zhang, Y-J, Shu, B 2007. The effects, in sheep, of dietary plant species and animal live weight on the faecal recovery rates of alkanes and the accuracy of intake and diet composition estimates obtained using alkanes as faecal markers. Journal of Agricultural Science 145, 87–94.CrossRefGoogle Scholar
Mayes RW and Duncan AJ 1999. New developments in the use of plant-wax markers to determine intake. In Emerging techniques for studying the nutrition of free ranging herbivores (ed. H Dove and SW Coleman). Satellite meeting of the 5th International Symposium on the Nutrition of Herbivores, San Antonio, Texas, USA (CD-ROM). http://cnrit.tamu.edu/conf/cd/web/post-online/sect0124/index.html.Google Scholar
Mayes, RW, Lamb, CS, Colgrove, PM 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107, 161–170.CrossRefGoogle Scholar
Mayes RW, Lamb CS and Colgrove PM 1988. Digestion and metabolism of dosed even-chain and herbage odd-chain n-alkanes in sheep. Proceedings of the 12th General Meeting of the European Grassland Federation, Dublin, Ireland, pp. 159–163.Google Scholar
Molina, DO, Matamoros, I, Pell, AN 2004. Accuracy of estimates of herbage intake of lactating cows using alkanes: comparison of two types of capsules. Animal Feed Science and Technology 114, 241–260.CrossRefGoogle Scholar
Moshtagi Nia, SA, Wittenberg, KM 2002. Evaluation of n-alkanes as markers for estimation of dry matter intake and digestibility in steers consuming all-forage or forage-concentrate diets. Canadian Journal of Animal Science 82, 419–425.CrossRefGoogle Scholar
Ohajuruka, OA, Palmquist, DL 1991. Evaluation of n-alkanes as digesta markers in dairy cows. Journal of Animal Science 69, 1726–1732.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute 2001. SAS version 8.2. SAS Institute Inc., Cary, NC.Google Scholar
Unal, Y, Garnsworthy, PC 1999. Estimation of intake and digestibility of forage-based diets in group-fed dairy cows using alkanes as markers. Journal of Agricultural Science, Cambridge 133, 419–425.CrossRefGoogle Scholar
Valiente, OL, Delgado, P, De Vega, A, Guada, JA 2003. Validation of the n-alkane technique to estimate intake, digestibility, and diet composition in sheep consuming mixed grain: roughage diets. Australian Journal of Agricultural Research 54, 693–702.CrossRefGoogle Scholar