Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-22T18:50:26.843Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression pattern of HIF1α mRNA in brain, heart and liver tissues of Tibet chicken embryos in hypoxia revealed with quantitative real-time PCR

Published online by Cambridge University Press:  01 November 2007

L. F. Zhang ,
L. S. Lian ,
C. J. Zhao ,
J. Y. Li ,
H. G. Bao  and
Ch. Wu
L. F. Zhang
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
L. S. Lian
Affiliation:
College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
C. J. Zhao
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
J. Y. Li
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
H. G. Bao
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Ch. Wu*
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
*
E-mail: [email protected]
Get access

Abstract

The problem of hypoxia adaptation in high altitudes is an unsolved brainteaser in the field of the life sciences. As one of the best chicken breeds with adaptability to highland environment, the Tibet chicken, is genetically different from lowland chicken breeds. It is well known that hypoxia has significant impact on growth by inducing the expression of the alpha subunit of the heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1α). In the present study, HIF1α expression in brain, heart and liver tissues of the Tibet and Dwarf Recessive White chicken embryos were investigated at the different development stages of days 10, 17 and 20 by real-time PCR, and the expression pattern of HIF1α in chicken embryos of the two chicken breeds incubated under conditions of hypoxia (13% O2) and normoxia (21% O2) was studied. The incubation mortality of the Tibet chicken was lower than the Dwarf Recessive White chicken during the whole incubation in hypoxia, and the mRNA expression of HIF1α had presented the differences in three tissues. The results implied that the hypoxia adaptability of the Tibet chicken embryo was higher than the Dwarf Recessive White chicken, especially in the early stage (day 10) and at a later time (day 20) during the incubation period, but the mechanism of the oxygen transfer of embryos of mountain species is not completely understood, and hypoxia adaptability of the Tibetan chicken remains to be studied further.

Keywords

embryosexpressivityHIF1αhypoxiaTibet chicken
Type
Full Paper
Information
animal , Volume 1 , Issue 10 , November 2007 , pp. 1467 - 1471
Copyright
Copyright © The Animal Consortium 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Acker, T, Acker, H 2004. Cellular oxygen sensing need in CNS function: physiological and pathological implications. The Journal of Experimental Biology 207, 3171–3188.CrossRefGoogle ScholarPubMed
Appenzeller, O, Minko, T, Qualls, C, Pozharov, V, Gamboa, J, Gamboa, A, Wang, Y 2006. Gene expression, autonomic function and chronic hypoxia: lessons from the Andes. Clinical Autonomic Research 16, 217–222.CrossRefGoogle ScholarPubMed
Beall, CM 2007. Two routes to functional adaptation: Tibetan and Andean high-altitude natives. Proceedings of the National Academy of Sciences 104, 8655–8660.CrossRefGoogle ScholarPubMed
Chan, T, Burggren, W 2005. Hypoxic incubation creates differential morphological effects during specific developmental critical windows in the embryo of the chicken (Gallus gallus). Respiratory Physiology and Neurobiology 145, 251–263.CrossRefGoogle ScholarPubMed
Chen, XQ, Wang, SJ, Du, JZ, Chen, XC 2007. Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice. American Journal of Physiology, Regulatory, Integrative and Comparative Physiology 292, R516–R526.CrossRefGoogle ScholarPubMed
Crossley, DA, Burggren, WW, Altimiras, J 2003. Cardiovascular regulation during hypoxia in embryos of the domestic chicken Gallus gallus. American Journal of Physiology Regulatory, Integrative and Comparative Physiology 284, 219–226.CrossRefGoogle ScholarPubMed
Dolt, KS, Mishra, MK, Karar, J, Baig, MA, Ahmed, Z, Pasha, MA 2007. cDNA cloning, gene organization and variant specific expression of HIF-1α in high altitude yak (Bos grunniens). Gene 386, 73–80.CrossRefGoogle ScholarPubMed
French, SW 2005. The pathogenesis and significance of the urinary alcohol cycle in rats fed ethanol intragastrically. Alcoholism: Clinical and Experimental Research 29, 158S–161S.CrossRefGoogle ScholarPubMed
Gou, X, Li, N, Lian, L, Yang, D, Zhang, H, Wu, C 2005. Hypoxia adaptation and hemoglobin mutation in Tibetan chick embryo. Science in China Series C – Life Sciences 48 (6), 616–623.CrossRefGoogle ScholarPubMed
Huang, Y, Hickey, RP, Yeh, JL, Dadak, A, Young, LH, Johnson, RS, Giordano, FJ 2004. Cardiac myocyte-specific HIF-1α deletion alters vascularization, energy availability, calcium flux, and contractility in the normoxic heart. FASEB Journal 18, 1138–1140.CrossRefGoogle ScholarPubMed
Jiang, BH, Semenza, GL, Bauer, C, Marti, HH 1996. Hypoxia inducible factor 1 level vary exponentially over a physiologically relevant range of O2 tension. American Journal of Physiology 271, C1172–C1180.CrossRefGoogle Scholar
Kietzmann, T, Comsse, Y, Brechte, IK, Modaressi, S, Jungermann, 2001. Perivenous expression of mRNA of the three hypoxia inducible factor α-subunit, HIF-lα, HIF-2α and HIF-3α in rat liver. Biochemical Journal 354, 531–537.CrossRefGoogle Scholar
Lee, C, Kim, DW, Jeon, GS, Roh, EJ, Seo, JH, Wang, KC, Cho, SS 2001. Cerebellar alterations induced by chronic hypoxia: an immunohistochemical study using a chick embryonic model. Brain Research 901, 271–276.CrossRefGoogle ScholarPubMed
Lee, JW, Bae, SH, Jeong, JW, Kim, SH, Kim, KW 2004. Hypoxia-inducible factor (HIF-1) alpha: its protein stability and biological functions. Experimental and Molecular Medicine 36, 1–12.CrossRefGoogle ScholarPubMed
Morin, PJ, Storey, KB 2005. Cloning and expression of hypoxia-inducible factor 1a from the hibernating ground squirrel, Spermophilus tridecemlineatus. Biochimica et Biophysica Acta 1729, 32–40.CrossRefGoogle Scholar
Rissanen, E, Tranberg, HK, Sollid, J, Nilsson, GE, Nikinmaa, M 2006. Temperature regulates hypoxia inducible factor-1 in a poikilothermic vertebrate, crucian carp (Carassius carassius). Journal of Experimental Biology 209, 994–1003.CrossRefGoogle Scholar
Rossignol, F, de Laplanche, E, Mounier, R, Bonnefont, J, Cayre, A, Godinot, C, Simonnet, H, Clottes, E 2004. Natural antisense transcripts of HIF-1a are conserved in rodents. Gene 339, 121–130.CrossRefGoogle Scholar
Semenza, GL 1999. Regulation of mammalian O2 homeostasis by hypoxia-inducible factor 1. Annual Review of Cell Developmental Biology 15, 551–578.CrossRefGoogle ScholarPubMed
Shams, I, Nevo, E, Avivi, A 2005. Ontogenetic expression of erythropoietin and hypoxiainducible factor-1 alpha genes in subterranean blind mole rats. FASEB Journal 19 (2), 307–309.CrossRefGoogle ScholarPubMed
Shams, I, Avivi, A, Nevo, E 2004. Hypoxic stress tolerance of the blind subterranean mole rat: expression of erythropoietin and hypoxia inducible factor 1 alpha. Proceedings of the National Academy of Sciences 101, 9698–9703.CrossRefGoogle ScholarPubMed
Schuler, B, Lundby, C, Gassmann, M 2005. HIF-1 and the adaptation of man to high altitude. Zeitschrift für Sportmedizin und Sporttraumaologie 53, 82–87.Google Scholar
Statistical Analysis Systems Institute 2000. User’s guide SAS software version 8.02. SAS Inst. Inc., Cary, NC.Google Scholar
Takahashi, T, Sugishita, Y, Nojiri, T, Shimizu, T, Yao, A, Kinugawa, KI, Harada, K, Nagai, R 2001. Cloning of hypoxia-inducible factor 1a cDNA from Chick embryonic ventricular myocytes. Biochemical and Biophysical Research Communications 281, 1057–1062.CrossRefGoogle Scholar
Wenger, RH 2002. Cellular adaptation to hypoxia: O2-sensing protein hydroxylases hypoxia-inducible transcription factors and O2-regulated gene expression. FASEB Journal 16, 1151–1162.CrossRefGoogle ScholarPubMed
Wu, chX, Zhang, H, Gou, X 2005. Automatization hypoxia simulation hatching, China Patent (2004200668293), Beijing.Google Scholar
Zhao, TB, Ning, HX, Zhu, SS, Sun, P, Xu, SX, Chang, ZJ, Zhao, XQ 2004. Cloning of hypoxia-inducible factor 1a cDNA from a high hypoxia tolerant mammal-plateau pika (Ochotona curzoniae). Biochemical and Biophysical Research Communications 316, 565–572.CrossRefGoogle Scholar