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Expression of HMGR and corresponding cholesterol content in tissues of two pig breeds

Published online by Cambridge University Press:  01 October 2009

G.-F. Liu ,
W.-N. Fang ,
H.-C. Lin ,
X.-F. Wang ,
J.-L. Fu  and
A.-G. Wang
G.-F. Liu
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture, Beijing, 100193, China
W.-N. Fang
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
H.-C. Lin
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, 250100, China
X.-F. Wang
Affiliation:
Animal Husbandry & Veterinary Service Station, Beijing, 100107, China
J.-L. Fu*
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture, Beijing, 100193, China
A.-G. Wang*
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture, Beijing, 100193, China
*
E-mail: [email protected]
E-mail: [email protected]
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Abstract

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. To study the expression of HMGR and corresponding cholesterol content in liver, adipose and muscle, six Chinese local breed (Huai pig) and Landrace pigs were selected. The results indicated that significant differences of cholesterol content in adipose (P < 0.01), liver (P < 0.05) and muscle (P < 0.01) tissues were detected between pigs of differing genetic backgrounds. HMGR mRNA expression were noted for adipose, liver and muscle of the two vastly differing genetics. Moreover cholesterol content differed (P < 0.01) among tissues across breed. Likewise, HMGR mRNA expression was different between adipose and liver tissues, muscle and liver tissues in both breeds; however, no difference was noted between adipose and muscle tissues. Results from this study indicate that cholesterol content and HMGR mRNA expression are higher in Huai pig tissues suggesting this gene is expressed in a breed- and tissue-dependent manner in pigs. Understanding the causes of variation in HMGR gene expression may provide crucial information about cholesterol biosynthesis.

Keywords

3-hydroxy-3-methylglutaryl-CoA reductasecholesterolmRNA expressionpig
Type
Full Paper
Information
animal , Volume 3 , Issue 10 , October 2009 , pp. 1436 - 1441
Copyright
Copyright © The Animal Consortium 2009

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