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Exogenous genistein in late gestation: effects on fetal development and sow and piglet performance

Published online by Cambridge University Press:  14 March 2016

C. Farmer ,
P. Robertson ,
C. W. Xiao ,
C. Rehfeldt  and
C. Kalbe
C. Farmer*
Affiliation:
Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, 2000 College Street, Sherbrooke, QC J1M 0C8, Canada
P. Robertson
Affiliation:
Nutrition Research Division, Health Canada, Ottawa, ON K1A 0K9, Canada
C. W. Xiao
Affiliation:
Nutrition Research Division, Health Canada, Ottawa, ON K1A 0K9, Canada
C. Rehfeldt
Affiliation:
Institute of Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
C. Kalbe
Affiliation:
Institute of Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
*
† E-mail: [email protected]
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Abstract

Due to their functional similarity to estradiol, phytoestrogens could prove to be beneficial in late gestating sows. The goal of this study was to determine the impact of providing the phytoestrogen genistein during late pregnancy on the performance of sows and their litters. In total, 56 gilts were equally divided into the two following groups on day 90 of gestation: (1) controls (CTL); and (2) two daily i.m. injections of 220 mg of genistein (GEN). Treatments were carried out until farrowing. Jugular blood samples were collected from 16 gilts/treatment on days 89 and 110 of gestation, and on days 3 and 21 of lactation. Milk samples were also obtained from those sows on day 3 of lactation. A male piglet from 16 CTL and 15 GEN litters was slaughtered at 24 h postpartum and a blood sample was obtained. The liver, heart and visceral organs were weighed and the semitendinosus (ST) muscle was collected and carcass composition was determined. The treatment increased (P<0.05) the concentrations of genistein and daidzein in the plasma of gilts on day 110 of gestation and of genistein in the plasma of piglets at 24 h postpartum. It also increased IGF1 concentrations in gilts at the end of the treatment period (P<0.05). Genistein had no impact (P>0.1) on weight or backfat loss of sows during lactation, milk composition or weights of piglets. The pre-weaning mortality rate of piglets was very low (<7%), yet the odds ratio comparing CTL with GEN sows indicated almost twice as many chances of pre-weaning deaths occurring in litters from CTL than GEN sows. Weights of the piglet carcasses were similar for both treatments, as well as weights of the various organs and of the ST muscle (P>0.1). However, carcasses from GEN litters contained more fat than those from CTL litters (9.63% v. 8.34%, P<0.05). None of the biochemical properties of the ST muscle differed between groups (P>0.1). In conclusion, injecting gilts with 440 mg/day of genistein in late gestation increased IGF1 concentrations in gilts and carcass fat in neonatal piglets, but had minimal effect on muscle development of piglets at birth and on the performance of lactating sows and their litters.

Keywords

fetal developmentgenisteingestationlitter performancesows
Type
Research Article
Information
animal , Volume 10 , Issue 9 , September 2016 , pp. 1423 - 1430
Copyright
© The Animal Consortium and Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada 2016 

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References

Agriculture and Agri-Food Canada 1993. Recommended code of practice for the care and handling of farm animals – pigs. Publication no. 1898E. Agriculture and Agri-Food Canada, Ottawa, ON, Canada.Google Scholar
Association of Official Analytical Chemists (AOAC) 2005. Official methods of analysis international, 18th edition. AOAC, Arlington, VA, USA.Google Scholar
Doerge, DR, Churchwell, MI, Chang, HC, Newbold, RR and Delclos, KB 2001. Placental transfer of the soy isoflavone, genistein, following oral administration to Sprague-Dawley rats. Reproductive Toxicology 15, 105–110.Google Scholar
Doerge, DR, Twaddle, NC, Newbold, RR and Delclos, KB 2006. Lactational transfer of the soy isoflavone, genistein, in Sprague-Dawley rats consuming dietary genistein. Reproductive Toxicology 21, 307–312.CrossRefGoogle ScholarPubMed
Erkens, T, van Poucke, M, Vandesompele, J, Goossens, K, van Zeveren, A and Peelman, LJ 2006. Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGCIA . BMC Biotechnology 6, 41.CrossRefGoogle Scholar
Farmer, C, Palin, MF, Gilani, GS, Weiler, H, Vignola, M, Choudhary, RK and Capuco, AV 2010. Dietary genistein stimulates mammary hyperplasia in gilts. Animal 4, 454–465.Google Scholar
Farmer, C, Robertson, P and Gilani, GS 2013. Effects of dose and route of administration of genistein on isoflavone concentrations in post-weaned and gestating sows. Animal 7, 983–989.Google Scholar
Flachowsky, G, Hünerberg, M, Meyer, U, Kammerer, DR, Carle, R, Goerke, M and Eklund, M 2011. Isoflavone concentration of soybean meal from various origins and transfer of isoflavones into milk of dairy cows. Journal of Consumer Protection and Food Safety 6, 449–456.CrossRefGoogle Scholar
Ford, JA Jr, Clark, SG, Walters, EM, Wheeler, MB and Hurley, WL 2006. Estrogenic effects of genistein on reproductive tissues of ovariectomized gilts. Journal of Animal Science 84, 834–842.Google Scholar
Kalbe, C, Bérard, J, Porm, M, Rehfeldt, C and Bee, G 2013. Maternal L-arginine supplementation during early gestation affects foetal skeletal myogenesis in pigs. Livestock Science 157, 322–329.CrossRefGoogle Scholar
Kalbe, C, Mau, M and Rehfeldt, C 2008. Developmental changes and the impact of isoflavones on mRNA expression of IGF-I receptor, EGF receptor and related growth factors in porcine skeletal muscle cell cultures. Growth Hormone and IGF Research 18, 424–433.CrossRefGoogle ScholarPubMed
Kalbe, C, Mau, M, Wollenhaupt, K and Rehfeldt, C 2007. Evidence for estrogen receptor α and β expression in skeletal muscle of pigs. Histochemical Cellular Biology 127, 95–117.Google Scholar
Kowalski, AA, Graddy, LG, Vale-Cruz, DS, Choi, I, Katzenellenbogen, BS, Simmen, FA and Simmen, RCM 2002. Molecular cloning of porcine estrogen receptor-β complementary DNAs and developmental expression in periimplantation embryos. Biology of Reproduction 66, 760–769.Google Scholar
Kuhn, G, Hennig, U, Kalbe, C, Rehfeldt, C, Ren, MQ, Moors, S and Degen, GH 2004. Growth performance, carcass characteristics and bioavailability of isoflavones in pigs fed soy bean based diets. Archives of Animal Nutrition 58, 265–276.Google Scholar
Lewis, RW, Brooks, N, Milburn, GM, Soames, A, Stone, S, Hall, M and Ashby, J 2003. The effects of the phytoestrogen genistein on the postnatal development of the rat. Toxicological Sciences 71, 74–83.Google Scholar
Li, FN, Li, LL, Yang, HS, Yuan, XX, Zhang, B, Geng, MM, Xiao, CW and Yin, YL 2011. Regulation of soy isoflavones on weight gain and fat percentage: evaluation in a Chinese Guangxi minipig model. Animal 5, 1903–1908.Google Scholar
Liu, GT, Zheng, YL, Chen, WH, Chen, J and Han, ZK 1999. Effect of daidzen fed to pregnant sows on milk production and the levels of hormones in colostrum. Journal of Nanjing Agricultural University 22, 69–72.Google Scholar
Lösel, L, Franke, A and Kalbe, C 2013. Comparison of different skeletal muscles from growing domestic pigs and wild boars. Archives Animal Breeding 56, 766–777.CrossRefGoogle Scholar
Montani, C, Penza, M, Jeremic, M, Biasiotto, G, La Sala, G, De Felici, M, Ciana, P, Maggi, A and Di Lorenzo, D 2008. Genistein is an efficient estrogen in the whole-body throughout mouse development. Toxicological Sciences 104, 57–67.Google Scholar
Necaise, K, Moulton, K, Christiansen, D, Walters, K, Crenshaw, M, Scanes, C and Ryan, P 2008. Effects of soy-derived phytoestrogen and estradiol exposure on reproductive development in male neonatal pigs. Journal of Animal Science 86 (E-suppl. 2), 386.Google Scholar
Nogowski, L, Szkudelska, K, Szkudelski, T and Pruszynska-Oszmalek, E 2006. The effect of a phytoestrogen, genistein, on the hormonal and metabolic status of pregnant rats. Journal of Animal and Feed Sciences 15, 275–286.CrossRefGoogle Scholar
Oksbjerg, N, Petersen, JS, Sorensen, IL, Henckel, P, Vestergaard, M, Ertbjerg, P, Moller, AJ, Bejerholm, C and Stoier, S 2000. Long-term changes in performance and meat quality of Danish Landrace pigs: a study on a current compared with an unimproved genotype. Animal Science 71, 81–92.Google Scholar
Padilla-Banks, E, Jefferson, WN and Newbold, RR 2006. Neonatal exposure to the phytoestrogen genistein alters mammary gland growth and developmental programming of hormone receptor levels. Endocrinology 147, 4871–4882.Google Scholar
Paredes, SP, Kalbe, C, Jansman, AJ, Verstegen, MWA, van Hees, HMJ, Lösel, D, Gerrits, WJJ and Rehfeldt, C 2013. Predicted high-performing piglets exhibit more and larger skeletal muscle fibers. Journal of Animal Science 91, 5589–5598.Google Scholar
Peterson, GL 1977. Simplification of protein assay method of Lowry et al – which is more generally applicable. Analytical Biochemistry 83, 346–356.Google Scholar
Pfaffl, MW, Lange, IG, Daxenberger, A and Meyer, HHD 2001. Tissue-specific pattern of estrogen receptors (ER): quantification of ERα and ERβ mRNA with real-time RT-PCR. Acta Pathologica, Microbiologica et Immunologica Scandinavica 109, 345–355.CrossRefGoogle ScholarPubMed
Plante, PA, Laforest, JP and Farmer, C 2011. Effect of supplementing the diet of lactating sows with NuPro® on sow lactation performance and piglet growth. Canadian Journal of Animal Science 91, 295–300.CrossRefGoogle Scholar
Rehfeldt, C, Adamovic, I and Kuhn, G 2007. Effects of dietary daidzein supplementation of pregnant sows on carcass and meat quality and skeletal muscle cellularity of the progeny. Meat Science 75, 103–111.Google Scholar
Rehfeldt, C, Kalbe, C, Nurnberg, G and Mau, M 2009. Dose-dependent effects of genistein and daidzein on protein metabolism in porcine myotube cultures. Journal of Agriculture and Food Chemistry 57, 852–857.Google Scholar
Rehfeldt, C and Walther, K 1997. A combined assay for DNA, protein, and incorporated [3H] label in cultured muscle cells. Analytical Biochemistry 251, 294–297.Google Scholar
Ren, MQ, Kuhn, G, Wegner, J, Nürnberg, G, Chen, J and Ender, K 2001. Feeding daidzein to late pregnant sows influences the estrogen receptor beta and type 1 insulin-like growth factor receptor mRNA expression in newborn piglets. Journal of Endocrinology 170, 129–135.Google Scholar
Robert, S, de Passillé, AMB, St-Pierre, N, Dubreuil, P, Pelletier, G, Petitclerc, D and Brazeau, P 1989. Effect of the stress of injection on the serum concentrations of cortisol, prolactin, and growth hormone in gilts and lactating sows. Canadian Journal of Animal Science 69, 663–672.CrossRefGoogle Scholar
SAS 1998. SAS/STAT user’s guide (version 6), 4th edition. SAS Institute Inc., Cary, NC, USA.Google Scholar
Sepehr, E, Robertson, P, Gilani, GS, Cooke, G and BP-Y, Lau 2006. An accurate and reproducible method for the quantitative analysis of isoflavones and their metabolites in rat plasma using liquid chromatography/mass spectrometry combined with photodiode array detection. Journal of AOAC (Association of Analytical Chemists) International 89, 1158–1167.Google Scholar
Sun Hwang, C, Seok Kwak, H, Jae Lim, H, Hee Lee, S, Soon Kang, Y, Boo Choe, T, Gil Hur, H and Ok Han, K 2006. Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration. Journal of Steroid Biochemistry and Molecular Biology 101, 246–253.Google Scholar