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Egg storage duration and hatch window affect gene expression of nutrient transporters and intestine morphological parameters of early hatched broiler chicks

Published online by Cambridge University Press:  07 December 2015

S. Yalcin ,
I. Gursel ,
G. Bilgen ,
G. T. Izzetoglu ,
B. H. Horuluoglu  and
G. Gucluer
S. Yalcin*
Affiliation:
Animal Science Department, Faculty of Agriculture, Ege University, 35100 Izmir, Turkey
I. Gursel
Affiliation:
THORLAB, Molecular Biology and Genetic Department, Science Faculty, Bilkent University, 06800 Ankara, Turkey
G. Bilgen
Affiliation:
Animal Science Department, Faculty of Agriculture, Ege University, 35100 Izmir, Turkey
G. T. Izzetoglu
Affiliation:
Biology Department, Faculty of Science, Ege University, 35100 Izmir, Turkey
B. H. Horuluoglu
Affiliation:
THORLAB, Molecular Biology and Genetic Department, Science Faculty, Bilkent University, 06800 Ankara, Turkey
G. Gucluer
Affiliation:
THORLAB, Molecular Biology and Genetic Department, Science Faculty, Bilkent University, 06800 Ankara, Turkey
*
†E-mail: [email protected]
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Abstract

In recent years, researchers have given emphasis on the differences in physiological parameters between early and late hatched chicks within a hatch window. Considering the importance of intestine development in newly hatched chicks, however, changes in gene expression of nutrient transporters in the jejunum of early hatched chicks within a hatch window have not been studied yet. This study was conducted to determine the effects of egg storage duration before incubation and hatch window on intestinal development and expression of PepT1 (H+-dependent peptide transporter) and SGLT1 (sodium–glucose co-transporter) genes in the jejunum of early hatched broiler chicks within a 30 h of hatch window. A total of 1218 eggs obtained from 38-week-old Ross 308 broiler breeder flocks were stored for 3 (ES3) or 14 days (ES14) and incubated at the same conditions. Eggs were checked between 475 and 480 h of incubation and 40 chicks from each egg storage duration were weighed; chick length and rectal temperature were measured. The chicks were sampled to evaluate morphological parameters and PepT1 and SGLT1 expression. The remaining chicks that hatched between 475 and 480 h were placed back in the incubator and the same measurements were conducted with those chicks at the end of hatch window at 510 h of incubation. Chick length, chick dry matter content, rectal temperature and weight of small intestine segments increased, whereas chick weight decreased during the hatch window. The increase in the jejunum length and villus width and area during the hatch window were higher for ES3 than ES14 chicks. PepT1 expression was higher for ES3 chicks compared with ES14. There was a 10.2 and 17.6-fold increase in PepT1 and SGLT1 expression of ES3 chicks at the end of hatch window, whereas it was only 2.3 and 3.3-fold, respectively, for ES14 chicks. These results suggested that egg storage duration affected development of early hatched chicks during 30 h of hatch window. It can be concluded that the ES14 chicks would be less efficiently adapted to absorption process for carbohydrates and protein than those from ES3 at the end of the hatch window.

Keywords

incubationchickshatch windowegg storagenutrient transporters
Type
Research Article
Information
animal , Volume 10 , Issue 5 , May 2016 , pp. 805 - 811
Copyright
© The Animal Consortium 2015 

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References

Careghi, C, Tona, K, Onagbesan, O, Buyse, J, Decuypere, E and Bruggeman, V 2005. The effects of the spread of hatch and interaction with delayed feed access after hatch on broiler performance until seven days of age. Poultry Science 84, 1314–1320.CrossRefGoogle ScholarPubMed
Chen, H, Pan, YX, Wong, EA, Bloomquist, JR and Webb, KE 2002. Molecular cloning and functional expression of a chicken intestinal peptide transporter (cPepT1) in Xenopus oocytes and Chinese Hamster ovary cells. Journal of Nutrition 132, 387–393.CrossRefGoogle ScholarPubMed
Chen, H, Pan, YX, Wong, EA and Webb, KE 2005. Dietary protein level and stage of development affect expression of an intestinal peptide transporter (cPepT1) in chickens. Journal of Nutrition 135, 193–198.CrossRefGoogle ScholarPubMed
Christensen, VL, Wineland, MJ, Fasenko, GM and Donaldson, WE 2001. Egg storage effects on plasma glucose and supply and demand tissue glycogen concentrations of broiler embryos. Poultry Science 80, 1729–1735.CrossRefGoogle ScholarPubMed
Christensen, VL, Wineland, MJ, Fasenko, GM and Donaldson, WE 2002. Egg storage alters weight of supply and demand organs of broiler chicken embryos. Poultry Science 81, 1738–1743.CrossRefGoogle ScholarPubMed
Decuypere, E and Bruggeman, V 2007. The endocrine interface of environmental and egg factors affecting chick quality. Poultry Science 86, 1037–1042.CrossRefGoogle ScholarPubMed
Decuypere, E, Tona, K, Bruggeman, V and Bamelis, F 2001. The day-old chick, a crucial hinge between breeders and broilers. World’s Poultry Science Journal 57, 127–138.CrossRefGoogle Scholar
Geyra, A, Uni, Z and Sklan, D 2001. Enterocyte dynamics and mucosal development in the post hatch chick. Poultry Science 80, 776–782.CrossRefGoogle Scholar
Gilbert, ER, Li, H, Emmerson, DA, Webb, KE and Wong, EA 2007. Developmental regulation of nutrient transporter and enzyme mRNA abundance in the small intestine of broilers. Poultry Science 86, 1739–1753.CrossRefGoogle ScholarPubMed
Hamidu, JA, Uddin, Z, Fasenko, GM, Guan, LL and Barreda, DR 2011. Broiler egg storage induces cell death and influences embryo quality. Poultry Science 90, 1749–1757.CrossRefGoogle ScholarPubMed
Ihara, T, Tsujikawa, T, Fujiyama, Y and Bamba, T 2000. Regulation of PepT1 peptide transporter expression in the rat small intestine under malnourished conditions. Digestion 61, 59–67.CrossRefGoogle ScholarPubMed
Lamot, DM, van de Linde, B, Molenaar, R, van der Pol, CW, Wijtten, PJA, Kemp, B and van den Brand, H 2014. Effects of moment of hatch and feed access on chicken development. Poultry Science 93, 2604–2614.CrossRefGoogle ScholarPubMed
Li, H, Gilbert, ER, Zhang, Y, Crasta, O, Emmerson, D, Webb, KE and Wong, EA 2008. Expression profiling of the solute carrier gene family in chicken intestine from the late embryonic to early post-hatch stages. Animal Genetics 39, 407–424.CrossRefGoogle ScholarPubMed
Meijerhof, R 1992. Pre-incubation holding of hatching eggs. World’s Poultry Science Journal 48, 57–68.CrossRefGoogle Scholar
Meijerhof, R, Noordhuzen, JP and Leenstra, FR 1994. Influence of preincubation treatment on hatching results of broiler breeder eggs produced at -1 and /3 weeks of age. British Poultry Science 35, 249–257.CrossRefGoogle ScholarPubMed
Miska, K, Fetterer, RH and Wong, EA 2014. The mRNA expression of amino acid transporters, aminopeptidase N, and the di- and tri-peptide transporter PepT1 in the embryo of the domesticated chicken (Gallus gallus) shows developmental regulation. Poultry Science 93, 2262–2270.CrossRefGoogle ScholarPubMed
Moran, ET 1985. Digestion and absorption of carbohydrates in fowl and events through perinatal development. Journal of Nutrition 115, 665–674.CrossRefGoogle ScholarPubMed
Mott, CR, Siegel, PB, Webb, KE and Wong, EA 2008. Gene expression of nutrient transporters in the small intestine of chickens from lines divergently selected from high or low juvenile body weight. Poultry Science 87, 2215–2224.CrossRefGoogle ScholarPubMed
Noy, Y and Sklan, D 1999. Energy utilization in newly hatched chicks. Poultry Science 78, 1750–1756.CrossRefGoogle ScholarPubMed
Noy, Y and Sklan, D 2001. Yolk and exogenous feed utilization in the posthatch chick. Poultry Science 80, 1490–1495.CrossRefGoogle ScholarPubMed
Reijrink, IAM, Meijerhof, R, Kemp, B, Graat, EAM and van den Brand, H 2009. Influence of prestorage incubation on embryonic development, hatchability, and chick quality. Poultry Science 88, 2649–2660.CrossRefGoogle ScholarPubMed
SAS Institute 2003. JMP Version 5.01. SAS Institute, Inc., Cary, NC, USA.Google Scholar
Schokker, D, Hoekman, AJW, Smits, MA and Rebel, JMJ 2009. Gene expression patterns associated with chicken jejunal development. Developmental and Comparative Immunology 33, 1156–1164.CrossRefGoogle ScholarPubMed
Schulte-Drüggelte, R 2011. Recommendations for hatching egg handling and storage. Lohmann Information 46, 55–58.Google Scholar
Sklan, D, Geyra, A, Tako, E, Gal-Gerber, O and Uni, Z 2003. Ontogeny of brush border carbohydrate digestion and uptake in the chick. British Journal of Nutrition 89, 747–753.CrossRefGoogle ScholarPubMed
Speier, JS, Yadgary, L, Uniz, Z and Wong, EA 2012. Gene expression of nutrient transporters and digestive enzymes in the yolk sac membrane and small intestine of the developing embryonic chick. Poultry Science 91, 1941–1949.CrossRefGoogle ScholarPubMed
Tona, K, Bamelis, F, De Ketelaer, B, Bruggeman, V, Moraes, VMB, Buyse, J, Onagbesan, O and Decuypere, E 2003. Effects of egg storage time on spread of hatch, chick quality, and chick juvenile growth. Poultry Science 82, 736–741.CrossRefGoogle ScholarPubMed
Uddin, Z and Hamidu, JA 2014. Prolong egg storage affects broiler breeder embryonic metabolism and chick quality. Journal of Animal Advances 4, 973–977.CrossRefGoogle Scholar
Uni, Z, Ganot, S and Sklan, D 1998. Posthatch development of mucosal function in the broiler small intestine. Poultry Science 77, 75–82.CrossRefGoogle ScholarPubMed
Uni, Z, Tako, E, Gal-Garber, O and Sklan, D 2003. Morphological, molecular and functional changes in the chicken small intestine of the late-term embryo. Poultry Science 82, 1747–1754.CrossRefGoogle ScholarPubMed
van de Ven, LJF, van Wagenberg, AV, Debonne, M, Decuypere, E, Kemp, B and van den Brand, H 2011. Hatching system and time effects on broiler physiology and posthatch growth. Poultry Science 90, 1267–1275.CrossRefGoogle ScholarPubMed
van de Ven, LJF, van Wagenberg, AV, Decuypere, E, Kemp, B and van den Brand, H 2013. Perinatal broiler physiology between hatching and chick collection in 2 hatching systems. Poultry Science 92, 1050–1061.CrossRefGoogle ScholarPubMed
Yadgari, L, Yair, R and Uni, Z 2011. The chick embryo yolk sac membrane expresses nutrient transported and digestive enzyme genes. Poultry Science 90, 410–416.CrossRefGoogle Scholar
Yalcin, S, Izzetoglu, GT and Aktaş, A 2013. Effects of breeder age and egg weight on morphological changes in the small intestine of chicks during the hatch window. British Poultry Science 54, 810–817.CrossRefGoogle ScholarPubMed
Yalcin, S. and Siegel, PB 2003. Developmental stability of broiler embryos in relation to length of egg storage prior to incubation. Japanese Poultry Science 40, 298–308.CrossRefGoogle Scholar