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Effects of feeding transgenic corn with mCry1Ac or maroACC gene to laying hens for 12 weeks on growth, egg quality and organ health

Published online by Cambridge University Press:  26 February 2016

R. Q. Zhong ,
L. Chen ,
L. X. Gao ,
L. L. Zhang ,
B. Yao ,
X. G. Yang  and
H. F. Zhang
R. Q. Zhong
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China
L. Chen
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China
L. X. Gao
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China
L. L. Zhang
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China
B. Yao
Affiliation:
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 100081, China
X. G. Yang
Affiliation:
Key Laboratory of Trace Element Nutrition MOH, National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, No. 29 Nanwei Road, Xicheng District, Beijing 100050, China
H. F. Zhang*
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China
*
†E-mail: [email protected]
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Abstract

The objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mCry1Ac gene from Bacillus thuringiensis strain (BT-799) and the maroACC gene from Agrobacterium tumefaciens strain (CC-2), respectively, on growth, egg quality and organ health indicators. Expression of the mCry1Ac gene confers resistance to Pyrausta nubilalis and the maroACC gene confers tolerance to herbicides. Healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assigned to one of four corn–soybean meal dietary treatments (8 cages/treatment) formulated with the following corn: non-transgenic near-isoline control corn (control), BT-799 corn, CC-2 corn and commercially available non-transgenic reference corn (reference). The experiment was divided into three 4-week phases (week 1 to 4, week 5 to 8 and week 9 to 12), during which hens were fed mash diets. Performance (BW, feed intake and egg production) and egg quality were determined. Following slaughter at the end of 12 weeks of feeding (n=8/treatment), carcass yield and organ weights (heart, liver, spleen, lung, kidneys, stomach and ovary) were recorded; organs and intestines were sampled for histological analysis. Analysis of serum biochemistry parameters to assess the liver and kidney function were performed. No differences in BW, egg production and production efficiency were observed between hens consuming the control diet and hens consuming the BT-799 or CC-2 diet. Haugh unit measures and egg component weights were similar between the control and test groups. Carcass yield was not affected by the diet treatment. Similar organosomatic indices and serum parameters did not indicate the characteristics of organ dysfunction. All observed values of the BT-799 and CC-2 groups were within the calculated tolerance intervals. This research indicates that the performance, egg quality, organ health and carcass yield of laying hens fed diets containing the BT-799 or CC-2 corn line were similar to that of laying hens fed diets formulated with the non-transgenic near-isoline corn with comparable genetic backgrounds.

Keywords

maroACCmCry1Aclaying hen performanceegg qualityorgan health
Type
Research Article
Information
animal , Volume 10 , Issue 8 , August 2016 , pp. 1280 - 1287
Copyright
© The Animal Consortium 2016 

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Footnotes

a

Both the authors contributed equally to this work and should be therefore considered as first coauthors.

References

Aeschbacher, K, Messikommer, R, Meile, L and Wenk, C 2005. Bt176 corn in poultry nutrition: physiological characteristics and fate of recombinant plant DNA in chickens. Poultry Science 84, 385–394.CrossRefGoogle ScholarPubMed
Asli, MM, Hosseini, SA, Lotfollahian, H and Shariatmadari, F 2007. Effect of probiotics, yeast, vitamin E and vitamin C supplements on performance and immune response of laying hens during high environmental temperature. International Journal of Poultry Science 6, 895–900.CrossRefGoogle Scholar
Association of Official Analytical Chemists (AOAC) 2000. Official methods of analysis, 17th edition. AOAC, Gaithersburg, MD, USA.Google Scholar
Association of Official Analytical Chemists (AOAC) 2007. Official methods of analysis, 18th edition. AOAC, Gaithersburg, MD, USA.Google Scholar
Boone, L, Meyer, D, Cusick, P, Ennulat, D, Bolliger, AP, Everds, N, Meador, V, Elliott, G, Honor, D, Bounous, D and Jordan, H 2005. Selection and interpretation of clinical pathology indicators of hepatic injury in preclinical studies. Veterinary Clinical Pathology 34, 182–188.CrossRefGoogle ScholarPubMed
Brake, J and Vlachos, D 1998. Evaluation of transgenic event 176 ‘Bt’ corn in broiler chickens. Poultry Science 77, 648–653.CrossRefGoogle ScholarPubMed
Buzoianu, SG, Walsh, MC, Rea, MC, Cassidy, JP, Ross, RP, Gardiner, GE and Lawlor, PG 2012. Effect of feeding genetically modified Bt MON810 maize to ~40 day old pigs for 110 days on growth and health indicators. Animal 10, 1609–1619.CrossRefGoogle Scholar
Er, D, Wang, Z, Cao, J and Chen, Y 2007. Effect of monochromatic light on the egg quality of laying hens. Journal of Applied Poultry Research 16, 605–612.CrossRefGoogle Scholar
Ganessunker, D, Gaskins, HR, Zuckermann, FA and Donovan, SM 1999. Total parenteral nutrition alters molecular and cellular indices of intestinal inflammation in neonatal piglets. Journal of Parenteral and Enteral Nutrition 23, 337–344.Google ScholarPubMed
Graybill, FA 1976. Theory and application of the linear model. Duxbury Press, North Scituate, MA, USA.Google Scholar
Gusta, M, Smyth, SJ, Belcher, K, Phillips, PWB and Castle, D 2011. Economic benefits of genetically-modified herbicide-tolerant canola for producers. AgBioForum 14, 1–13.Google Scholar
Halle, I and Flachowsky, G 2014. A four-generation feeding study with genetically modified (Bt) maize in laying hen. Journal of Animal and Feed Sciences 23, 58–63.CrossRefGoogle Scholar
Holst, DO 1973. Holst filtration apparatus for Van Soest detergent fiber analysis. Journal of AOAC International 56, 1352–1356.CrossRefGoogle Scholar
Jacobs, CM, Utterback, PL, Parsons, CM, Rice, D, Smith, B, Hinds, M, Liebergesell, M and Sauber, T 2008. Performance of laying hens fed diets containing DAS-59122-7 maize grain compared with diets containing non-transgenic maize grain. Poultry Science 87, 475–479.CrossRefGoogle Scholar
James, C 2014. Global status of commercialized biotech/GM crops: 2014. ISAAA brief no. 49. ISAAA, Ithaca, NY, USA.Google Scholar
Johansson, ME and Hansson, GC 2014. Is the intestinal goblet cell a major immune cell? Cell Host and Microbe 15, 251–252.CrossRefGoogle ScholarPubMed
Kaneko, JJ, Harvey, JW and Bruss, ML 2008. Clinical biochemistry of domestic animals, 6th edition. Academic Press, New York, NY, USA.Google Scholar
Kilic, A and Akay, MT 2008. A three generation study with genetically modified Bt corn in rats: biochemical and histopathological investigation. Food and Chemical Toxicology 46, 1164–1170.CrossRefGoogle ScholarPubMed
Kim, YS and Ho, SB 2010. Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports 12, 319–330.CrossRefGoogle ScholarPubMed
Mair, C, Plitzner, C, Pfaffl, MW, Schedle, K, Meyer, HH and Windisch, W 2010. Inulin and probiotics in newly weaned piglets: effects on intestinal morphology, mRNA expression levels of inflammatory marker genes and haematology. Archives of Animal Nutrition 64, 304–321.CrossRefGoogle ScholarPubMed
McNaughton, J, Roberts, M, Rice, D, Smith, B, Hinds, M, Delaney, B, Iiams, C and Sauber, T 2011. Nutritional equivalency evaluation of transgenic maize grain from event DP-Ø9814Ø-6 and transgenic soybeans containing event DP-356Ø43-5: laying hen performance and egg quality measures. Poultry Science 90, 377–389.CrossRefGoogle ScholarPubMed
Mejia, L, Jacobs, CM, Utterback, PL, Parsons, CM, Rice, D, Sanders, C, Smith, B, Iiams, C and Sauber, T 2010. Evaluation of nutritional equivalency of soybean meal with the genetically modified trait DP-3Ø5423-1 when fed to laying hens. Poultry Science 89, 2634–2639.CrossRefGoogle ScholarPubMed
National Research Council 1994. Nutrient requirements of poultry, 9th revised edition. The National Academies Press, Washington, DC, USA.Google Scholar
Rasmussen, MA, Cutler, SA, Wilhems, K and Scanes, CG 2007. Effects of Bt (Bacillus thuringiensis) corn on reproductive performance in adult laying hens. International Journal of Poultry Science 6, 169–171.CrossRefGoogle Scholar
Scheideler, SE, Rice, D, Smith, B, Dana, G and Sauber, T 2008. Evaluation of nutritional equivalency of corn grain from DAS-Ø15Ø7-1 (Herculex* I) in the diets of laying hens. Journal of Applied Poultry Research 17, 383–389.CrossRefGoogle Scholar
Stevens, A, Lowe, JS and Young, B 2002. Wheater’s basic histopathology, 4th edition. Churchill Livingstone, London, UK.Google Scholar
Taylor, ML, Hartnell, G, Nemeth, M, Karunanandaa, K and George, B 2005. Comparison of broiler performance when fed diets containing corn grain with insect-protected (corn rootworm and European corn borer) and herbicide-tolerant (glyphosate) traits, control corn, or commercial reference corn – revisited. Poultry Science 84, 1893–1899.CrossRefGoogle ScholarPubMed
Van Den Brand, H, Parmentier, HK and Kemp, B 2004. Effects of housing system (outdoor vs cages) and age of laying hens on egg characteristics. British Poultry Science 45, 745–752.CrossRefGoogle ScholarPubMed
Walsh, MC, Buzoianu, SG, Gardiner, GE, Rea, MC, Ross, RP, Cassidy, JP and Lawlor, PG 2012. Effects of short-term feeding of Bt MON810 maize on growth performance, organ morphology and function in pigs. British Journal of Nutrition 107, 364–371.CrossRefGoogle ScholarPubMed