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Effects of different sources of physically effective fiber on rumen microbial populations

Published online by Cambridge University Press:  14 September 2015

C. N. Shaw
Affiliation:
Department of Animal Sciences, The Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210USA
M. Kim
Affiliation:
Department of Animal Sciences, The Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210USA
M. L. Eastridge
Affiliation:
Department of Animal Sciences, The Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210USA
Z. Yu*
Affiliation:
Department of Animal Sciences, The Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210USA
*
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Abstract

Physically effective fiber is needed by dairy cattle to prevent ruminal acidosis. This study aimed to examine the effects of different sources of physically effective fiber on the populations of fibrolytic bacteria and methanogens. Five ruminally cannulated Holstein cows were each fed five diets differing in physically effective fiber sources over 15 weeks (21 days/period) in a Latin Square design: (1) 44.1% corn silage, (2) 34.0% corn silage plus 11.5% alfalfa hay, (3) 34.0% corn silage plus 5.1% wheat straw, (4) 36.1% corn silage plus 10.1% wheat straw, and (5) 34.0% corn silage plus 5.5% corn stover. The impact of the physically effective fiber sources on total bacteria and archaea were examined using denaturing gradient gel electrophoresis. Specific real-time PCR assays were used to quantify total bacteria, total archaea, the genus Butyrivibrio, Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and three uncultured rumen bacteria that were identified from adhering ruminal fractions in a previous study. No significant differences were observed among the different sources of physical effective fiber with respect to the microbial populations quantified. Any of the physically effective fiber sources may be fed to dairy cattle without negative impact on the ruminal microbial community.

Type
Research Article
Copyright
© The Animal Consortium 2015 

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References

Benson, AK, Kelly, SA, Legge, R, Ma, F, Low, SJ, Kim, J, Zhang, M, Oh, PL, Nehrenberg, D, Hua, K, Kachman, SD, Moriyama, EN, Walter, J, Peterson, DA and Pomp, D 2010. Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. Proceedings of the National Academy of Sciences of the United States of America 107, 1893318938.CrossRefGoogle ScholarPubMed
Bourquin, LD and Fahey, GC 1994. Ruminal digestion and glycosyl linkage patterns of cell wall components from leaf and stem fractions of alfalfa, orchardgrass, and wheat straw. Journal Animal Science 72, 13621374.Google Scholar
Brulc, JM, Antonopoulos, DA, Miller, MEB, Wilson, MK, Yannarell, AC, Dinsdale, EA, Edwards, RE, Frank, ED, Emerson, JB, Wacklin, P, Coutinho, PM, Henrissat, B, Nelson, KE and White, BA 2009. Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. Proceedings of the National Academy of Sciences of the United States of America 106, 19481953.Google Scholar
Chen, XL, Wang, JK, Wu, YM and Liu, JX 2008. Effects of chemical treatments of rice straw on rumen fermentation characteristics, fibrolytic enzyme activities and populations of liquid- and solid-associated ruminal microbes in vitro. Animal Feed Science and Technology 141, 114.Google Scholar
Cherney, DJ, Mertens, DR and Moore, JE 1991. Fluid and particulate retention times in sheep as influenced by intake level and forage morphological composition. Journal of Animal Science 69, 413422.Google Scholar
Cressman, MD, Yu, Z, Nelson, MC, Moeller, SJ, Lilburn, MS and Zerby, HN 2010. Interrelations between the microbiotas in the litter and in the intestines of commercial broiler chickens. Applied and Environmental Microbiology 76, 65726582.CrossRefGoogle ScholarPubMed
Enemark, JM 2008. The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): a review. The Veterinary Journal 176, 3243.CrossRefGoogle ScholarPubMed
Jung, HG and Allen, MS 1995. Characteristics of plant cell walls affecting intake and digestibility of forages by ruminants. Journal of Animal Science 73, 27742790.Google Scholar
Kim, M, Morrison, M and Yu, Z 2011. Status of the phylogenetic diversity census of ruminal microbiomes. FEMS Microbiology Ecology 76, 4963.CrossRefGoogle ScholarPubMed
Kim, M and Yu, Z 2012. Quantitative comparisons of select cultured and uncultured microbial populations in the rumen of cattle fed different diets. Journal of Animal Science and Biotechnology 3, 28.Google Scholar
Koike, S and Kobayashi, Y 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiology Letters 204, 361366.Google Scholar
Krause, DO, Denman, SE, Mackie, RI, Morrison, M, Rae, AL, Attwood, GT and McSweeney, CS 2003. Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics. FEMS Microbiology Reviews 27, 663693.CrossRefGoogle ScholarPubMed
Lane, DJ 1991. 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics (ed. E Stackebrandt and M Goodfellow), pp. 115175. John Wiley and Sons, New York, NY.Google Scholar
Larue, R, Yu, Z, Parisi, VA, Egan, AR and Morrison, M 2005. Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing. Environmental Microbiology 7, 530543.Google Scholar
Li, Y and Meng, Q 2006. Effect of different types of fibre supplemented with sunflower oil on ruminal fermentation and production of conjugated linoleic acids in vitro. Archives of Animal Nutrition 60, 402411.Google Scholar
Michalet-Doreau, B, Fernandez, I, Peyron, C, Millet, L and Fonty, G 2001. Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents. Reproduction Nutrition Development 41, 187194.Google Scholar
Nadkarni, MA, Martin, FE, Jacques, NA and Hunter, N 2002. Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148, 257266.Google Scholar
Olubobokun, JA and Craig, WM 1990. Quantity and characteristics of microorganisms associated with ruminal fluid or particles. Journal of Animal Science 68, 33603370.Google Scholar
Poore, MH, Moore, JA, Swingle, RS, Eck, TP and Brown, WH 1991. Wheat straw or alfalfa hay in diets with 30% neutral detergent fiber for lactating Holstein cows. Journal of Dairy Science 74, 31523159.Google Scholar
Shinkai, T and Kobayashi, Y 2007. Localization of ruminal cellulolytic bacteria on plant fibrous materials as determined by fluorescence in situ hybridization and real-time PCR. Applied and Environmental Microbiology 73, 16461652.Google Scholar
Singh, KM, Tripathi, AK, Pandya, PR, Parnerkar, S, Rank, DN, Kothari, RK and Joshi, CG 2013. Use of real-time PCR technique in determination of major fibrolytic and non fibrolytic bacteria present in Indian Surti buffaloes (Bubalus bubalis). Polish Journal of Microbiology 62, 195200.Google Scholar
Starkey, RA, Gott, PA, Eastridge, ML, Oelker, ER, Sewell, AR, Mathew, B and Firkins, JL 2009. Differentiating effects of effective fiber sources on performance of lactating dairy cows. Journal of Dairy Science 91 (E-suppl. 1), 74.Google Scholar
Stiverson, J, Morrison, M and Yu, Z 2011. Populations of select cultured and uncultured bacteria in the rumen of sheep and the effect of diets and ruminal fractions. International Journal of Microbiology 2011, 750613.Google Scholar
Tajima, K, Aminov, RI, Nagamine, T, Matsui, H, Nakamura, M and Benno, Y 2001. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Applied and Environmental Microbiology 67, 27662774.CrossRefGoogle ScholarPubMed
Varga, GA and Kolver, ES 1997. Microbial and animal limitations to fiber digestion and utilization. The Journal of Nutrition 127, 819S823S.Google Scholar
Yu, Y, Lee, C, Kim, J and Hwang, S 2005. Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. Biotechnology and Bioengineering 89, 670679.CrossRefGoogle ScholarPubMed
Yu, Z, García-González, R, Schanbacher, FL and Morrison, M 2008. Evaluations of different hypervariable regions of archaeal 16S rRNA genes in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 74, 889893.Google Scholar
Yu, Z and Morrison, M 2004a. Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 70, 48004806.CrossRefGoogle ScholarPubMed
Yu, Z and Morrison, M 2004b. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 36, 808812.Google Scholar
Zebeli, Q, Aschenbach, JR, Tafaj, M, Boguhn, J, Ametaj, BN and Drochner, W 2012. Invited review: role of physically effective fiber and estimation of dietary fiber adequacy in high-producing dairy cattle. Journal of Dairy Science 95, 10411056.Google Scholar