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Effects of condensed tannins in wrapped silage bales of sainfoin (Onobrychis viciifolia) on in vivo and in situ digestion in sheep

Published online by Cambridge University Press:  07 September 2011

K. Theodoridou ,
J. Aufrère ,
D. Andueza ,
A. Le Morvan ,
F. Picard ,
J. Pourrat  and
R. Baumont
K. Theodoridou
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
J. Aufrère*
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
D. Andueza
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
A. Le Morvan
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
F. Picard
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
J. Pourrat
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
R. Baumont
Affiliation:
INRA Unité de Recherches sur les Herbivores, Centre de Clermont-Ferrand – Theix, 63122, Saint-Genès-Champanelle, France
*
†Email: [email protected]
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Abstract

The objective of this study was to characterize the condensed tannins (CTs) in wrapped silage bales of sainfoin (Onobrychis viciifolia) and examine their potential action on in vivo and in situ digestive characteristics in sheep. Silage was made from sainfoin, cut at two phenological stages. The first phenological stage, at which silage was made, was from the first vegetation cycle at the end of flowering and the second stage silage was made from regrowth, 5 weeks after the first cut, but before flowering. The silages made from the two phenological stages were fed to 12 rumen-fistulated sheep in a crossover design. Of the 12 sheep, six received polyethylene glycol (PEG) to bind with and remove the effects of CT, whereas the other six were dosed with water. Organic matter digestibility, total-tract N digestibility and N (N) balance were measured over 6 days. Kinetic studies were performed on total N, ammonia N (NH3-N) and volatile fatty acids (VFAs) in rumen fluid before and 1.5, 3 and 6 h after feeding. The kinetics of degradation of dry matter and N from Dacron bags suspended in the rumen were also determined. Biological activity of CT (protein-binding capacity) and CT concentration were greater for the silage made from sainfoin at the early flowering stage. Total-tract N digestibility was increased by the addition of PEG (P < 0.001) to the sainfoin silage before flowering (P < 0.001). CTs decreased N excretion in urine (P < 0.05) and increased faecal N excretion (P < 0.001), but had no effect on body N retention, which is beneficial for the animal. Ruminal N degradability was smaller in the presence of active CT (P < 0.001) at both phenological stages; however, soluble N (P = 0.2060) and NH3-N (P = 0.5225) concentrations in rumen fluid remained unchanged. The results of this experiment indicate that CT in the sainfoin retain their ability to affect the nutritive value of preserved forage legumes.

Keywords

feed valuein vivo measurementstanninspolyethylene glycolsainfoin silage
Type
Full Paper
Information
animal , Volume 6 , Issue 2 , 03 January 2012 , pp. 245 - 253
Copyright
Copyright © The Animal Consortium 2011

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References

Aharoni, Y, Gilboa, N, Silanikove, N 1998. Analysis of the suppressive effect of tannins on ruminal degradation by compartmental models. Animal Feed Science and Technology 71, 251–267.CrossRefGoogle Scholar
Albrecht, KA, Muck, RE 1991. Proteolysis in ensiled legumes that vary in tannin concentration. Crop Science 31, 464–469.CrossRefGoogle Scholar
Arrigo, Y 2009. Effet d'un apport croissant d'esparcette dans des rations d'ensilage de luzerne sur la valeur nutritive des rations chez le mouton. Schriftenreihe aus dem Institut für Nutztierwissenschaften der ETH Zürich 31, 120–123.Google Scholar
Aufrère, J, Michalet-Doreau, B 1983. In vivo digestibility and prediction of digestibility of some by-products. In Feeding value of by-products and their use by beef cattle (ed. ChV Boucqué, LO Fiems and BG Cottyn), pp. 25–33. Commision of the European Communities Publishing Brussels, Luxembourg.Google Scholar
Aufrère, J, Dudilieu, M, Poncet, C 2008. In vivo and in situ measurements of the digestive characteristics of sainfoin in comparison with lucerne fed to sheep as fresh forages at two growth stages and as hay. Animal 2, 1331–1339.CrossRefGoogle ScholarPubMed
Aufrère, J, Graviou, D, Baumont, R, Detour, A, Demarquilly, C 2000. Degradation in the rumen of proteins from fresh lucerne forage in various stages of growth and conserved as silage or hay. Annales de Zootechnie 49, 461–474.CrossRefGoogle Scholar
Barry, TN, McNabb, WC 1999. The implications of condensed tannins on the nutritive value of temperate forages fed to ruminants. British Journal of Nutrition 81, 263–272.CrossRefGoogle ScholarPubMed
Barry, TN, McNeill, DM, McNabb, WC 2001. Plant secondary compounds; their impact on forage nutritive value upon animal production. In Proceeding of the XIX International Grassland Congress. Brazilian Society of Animal Husbandry (ed. JA Gomide, WRS Mattos and SC da Silva), pp. 445–452. Piracicaba, Brazil.Google Scholar
Ben Salem, H 1998. Effets de l'Acacia cyanophylla Lindl. sur l'ingestion et la digestion des régimes destinés aux ovins. Rôle des tanins et perspectives d'amélioration de sa valeur alimentaire. PhD, University of Dijon, 252 pp.Google Scholar
Buckee, GK 1994. Determination of total nitrogen in barley, malt and beer by Kjeldahl procedures and the Dumas combustion method: collaborative trial. Journal of the Institute of Brewing 100, 57–64.CrossRefGoogle Scholar
Cavallarin, L, Antoniazzi, S, Borreani, G, Tabacco, E 2005. Effects of wilting and mechanical conditioning on proteolysis in sainfoin (Onobrychis viciifolia Scop) wilted herbage and silage. Journal of the Science of Food and Agriculture 85, 831–838.CrossRefGoogle Scholar
Conway, EJ 1957. Microdiffusion analysis and volumetric analysis error. Crosby Lockwood and Son, London.Google Scholar
Demarquilly, C, Jarrige, R 1973. The comparative nutritive value of grasses and legumes. Vâxtodling 28, 33–41.Google Scholar
Demarquilly, C, Chenost, M, Giger, S 1995. Pertes fécales et digestibilité des aliments et des rations. In Nutrition des ruminants domestiques (ed. R Jarrige, Y Ruckebusch, C Demarquilly, MH Farce and M Journet), pp. 601–647. INRA Editions, Paris, France.Google Scholar
Demarquilly, C, Dulphy, JP, Andrieu, JP 1998. Evolution de la valeur nutritive et de la valeur alimentaire des fourrages selon les techniques de récolte et de conservation: foin, ensilage, enrubannage. Fourrages 155, 349–369.Google Scholar
Dentinho, MTP, Bessa, RJB, Belo, CAC, Ribeiro, JMC 2006. Effect of the preservation method on the nutritive value of sulla (Hedysarum coronarium L.). In Sustainable grassland productivity (ed. J Lloveras, A Gonzales-Rodriguez, O Vasquez-Yanez, J Pineiro, O Santamaria, L Olea, MJ Poblaciones), vol. 11, pp. 252–254. caja de Badajoz, Spain.Google Scholar
Dulphy, JP, Demarquilly, C 1981. Problèmes particuliers aux ensilages. Prévision de la valeur nutritive des aliments des ruminants, pp. 81–104. INRA, France.Google Scholar
Dulphy, JP, Demarquilly, C, Baumont, R, Jailler, M, L'Hotelier, L, Dragomir, C 1999. Study of modes of preparation of fresh and conserved forages for measurement of their dry matter and nitrogen degradations in the rumen. Annales de Zootechnie 48, 275–287.CrossRefGoogle Scholar
Fraser, MD, Fychan, R, Jones, R 2000. Voluntary intake, digestibility and nitrogen utilization by sheep fed ensiled forage legumes. Grass and Forage Science 55, 271–279.CrossRefGoogle Scholar
Guo, X, Zhou, H, Yu, Z, Zhang, Y 2007. Changes in the distribution of nitrogen and plant enzymatic activity during ensilage of lucerne treated with different additives. Grass and Forage science 62, 35–43.CrossRefGoogle Scholar
Hervás, G, Frutos, P, Ramos, G, Giráldez, FJ, Mantecón, AR 2004. Intraruminal administration of two doses of quebracho tannins to sheep: effect on rumen degradation and total tract digestibility, faecal recovery and toxicity. Journal of Animal Feed Science 13, 111–120.CrossRefGoogle Scholar
Jouany, JP 1982. Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Sciences des Aliments 2, 131–144.Google Scholar
Kawas, JR, Jorgensen, NA, Lu, CD 1990. Influence of alfalfa maturity on feed intake and site of nutrient digestion in sheep. Journal of Animal Science 68, 4376–4386.CrossRefGoogle ScholarPubMed
Makkar, HPS, Blummel, M, Becker, K 1995. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques. British Journal of Nutrition 73, 897–913.CrossRefGoogle ScholarPubMed
Makkar, HPS, Mueller-Harvey, I, Hagerman, AE 2000. Quantification of tannins in tree Foliage: a laboratory manual for the FAO/IAEA working document. IAEA, Vienna, pp. 1–25.Google Scholar
Michalet-Doreau, B, Ould-Bah, MY 1989. Estimation of the extent of bacterial contamination in bag residues and its influence on in sacco measurements of forage nitrogen degradation in rumen. In XVI International Grassland Congress (ed. Association Française pour la Production Fourragère), vol. II, pp. 909–910. INRA, France.Google Scholar
Michalet-Doreau, B, Vérité, R, Chapoutot, P 1987. Methodology of estimating degradability in sacco of nitrogen in feed in the rumen. Bulletin Technique CRZV Theix 69, 5–7.Google Scholar
Min, BR, Batty, TN, Attwood, GT, McNabb, WC 2003. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages. Animal Feed Science and Technology 106, 3–19.CrossRefGoogle Scholar
Minnee, EMK, Woodward, SL, Waghorn, GC, Laboyrie, PG 2002. The effect of ensiling forage legumes on condensed tannins. Agronomy New Zealand 32/33, 117–119.Google Scholar
Mueller-Harvey, I 2006. Review. Unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture 86, 2010–2037.CrossRefGoogle Scholar
Noll, F 1974. L-lactate determination with LDH, GPT and NAD. In Methods of enzymatic analysis (ed. HU Bermeyer), vol. III, pp. 1475–1477. Academic Press, London, UK.Google Scholar
Ørskov, ER, McDonald, I 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science 92, 499–503.CrossRefGoogle Scholar
Pagan, S, Wolfe, RM, Terrill, TH, Muir, JP 2009. Effect of drying method and assay methodology on detergent fiber analysis in plants containing condensed tannins. Animal Feed Science and Technology 154, 119–124.CrossRefGoogle Scholar
Reed, JD 1986. Relationships among phenolics, insoluble proanthocyanidins and fiber in East African browse species. Journal of Range Management 39, 5–7.CrossRefGoogle Scholar
Rémond, B, Brugère, H, Poncet, C, Baumont, R 1995. Le contenu du réticulo-rumen. In Nutrition des ruminants domestiques (ed. R Jarrige, Y Ruckebusch, C Demarquilly, MH Farce and M Journet), pp. 253–298. INRA, Paris, France.Google Scholar
Salawu, MB, Acamovic, T, Stewart, CS, Hvelplund, T, Weisbjerg, MR 1999. The use of tannins as silage additives: effects on silage composition and mobile bags disappearance of dry matter and protein. Animal Feed Science and Technology 82, 243–259.CrossRefGoogle Scholar
Scharenberg, A, Arrigo, Y, Gutzwiller, A, Wyss, U, Hess, H, Kreuzer, M, Dohme, F 2007. Effect of feeding dehydrated and ensiled tanniniferous sainfoin (Onobrychis viciifolia) on nitrogen and mineral digestion and metabolism of lambs. Archives of Animal Nutrition 61, 390–405.CrossRefGoogle Scholar
Statistical Analysis Systems Institute (SAS) 2000. SAS version 8. SAS Institute Inc., Cary, NC, USA.Google Scholar
Theodoridou, K, Aufrere, J, Andueza, D, Montet, G, Baumont, R 2008. Valeur nutritive de deux variétés de sainfoin, cultivées en deux lieux différents et conservées en fourrage vert et en ensilage. 14e Rencontres Recherche Ruminants p. 287, INRA, Institut de l'Elevage, France.Google Scholar
Theodoridou, K, Aufrere, J, Andueza, D, Baumont, R 2009. Use of polyethylene glycol (PEG) to assess the effect of condensed tannins on nitrogen balance and digestibility in sheep fed fresh sainfoin (Onobrychis viciifolia). In Ruminant physiology digestion, metabolism, and effects of nutrition on reproduction and welfare (ed. Y Chilliard, F Glasser, Y Faulconnier, F Bocquier, I Veissier, M. Doreau), pp 96–97. Wageningen Academic Publishers, The Netherlands.Google Scholar
Theodoridou, K, Aufrère, J, Andueza, D, Pourrat, J, Le Morvan, A, Stringano, E, Mueller-Harvey, I, Baumont, R 2010. The effect of condensed tannins in fresh sainfoin (Onobrychis viciifolia) on in vivo and in situ digestion in sheep. Animal Feed Science and Technology 160, 23–38.CrossRefGoogle Scholar
Theodoridou, K, Aufrère, J, Andueza, D, Pourrat, J, Le Morvan, A, Picard, F, Stringano, E, Mueller-Harvey, I, Baumont, R 2011. Effect of plant development during first and second growth cycle on chemical composition, condensed tannins and nutritive value of three sainfoin (Onobrychis viciifolia) varieties and lucerne. Grass and Forage Science 66, 402–414. doi: 10.1111/j.1365-2494.2011.00798.x.CrossRefGoogle Scholar
Van Soest, PJ, Wine, RH 1967. Use of detergents in the analysis of fibrous feeds. IV Determination of plant cell-wall constituents. Journal Association of Official Analytical Chemists 50, 50–55.Google Scholar
Waghorn, GC 2008. Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production. Progress and challenges. Animal Feed Science and Technology 147, 116–139.CrossRefGoogle Scholar
Waghorn, GC, McNabb, WC 2003. Consequences of plant phenolic compounds for productivity and health of ruminants. Proceedings of the Nutrition Society 62, 383–392.CrossRefGoogle ScholarPubMed
Weatherburn, MW 1967. Phenol hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–974.CrossRefGoogle Scholar
Yáñez Ruiz, DR, Moumen, A, Martín García, AI, Molina Alcaide, E 2004. Ruminal fermentation and degradation patterns, protozoa population, and urinary purine derivatives excretion in goats and wethers fed diets based on two-stage olive cake: effect of PEG supply. Journal of Animal Science 82, 2023–2032.CrossRefGoogle ScholarPubMed