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Effect of the addition of IGF-I and vitamin E to stored boar semen

Published online by Cambridge University Press:  05 December 2012

M. F. B. Mendez ,
M. G. Zangeronimo ,
L. G. P. Rocha ,
B. G. Faria ,
B. A. Pereira ,
C. D. Fernandes ,
B. R. Chaves ,
L. D. S. Murgas  and
R. V. Sousa
M. F. B. Mendez
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
M. G. Zangeronimo*
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
L. G. P. Rocha
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
B. G. Faria
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
B. A. Pereira
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
C. D. Fernandes
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
B. R. Chaves
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
L. D. S. Murgas
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
R. V. Sousa
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
*
†E-mail: [email protected]
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Abstract

The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°C for 24 or 72 h, samples were warmed at 37°C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 μg/ml of vitamin E to diluted semen reduced (P < 0.01) the malondialdehyde (MDA) production in boar semen stored at 15°C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 37°C. In these conditions, IGF-I also reduced (P < 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced (P = 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 μg/ml of vitamin E reduces the MDA production in boar semen stored at 15°C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 37°C.

Keywords

α-tocopherolantioxidantboar spermatozoainsulin-like growth factor-Ilipid peroxidation
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 5 , May 2013 , pp. 793 - 798
Copyright
Copyright © The Animal Consortium 2012

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