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Effect of supplementing coconut or krabok oil, rich in medium-chain fatty acids on ruminal fermentation, protozoa and archaeal population of bulls

Published online by Cambridge University Press:  18 November 2013

P. Panyakaew ,
N. Boon ,
G. Goel ,
C. Yuangklang ,
J. Th. Schonewille ,
W. H. Hendriks  and
V. Fievez
P. Panyakaew
Affiliation:
Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands Department of Animal Science, Faculty of Natural Resources, Rajamangala University of Technology Isan, Sakon-Nakhon Campus, Sakon Nakhon 47160Thailand Department of Animal Production, Laboratory for Animal Nutrition and Animal Product Quality (Lanupro), Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
N. Boon
Affiliation:
Department of Biochemical and Microbial Technology, Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium
G. Goel
Affiliation:
Department of Animal Production, Laboratory for Animal Nutrition and Animal Product Quality (Lanupro), Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, India
C. Yuangklang
Affiliation:
Department of Animal Science, Faculty of Natural Resources, Rajamangala University of Technology Isan, Sakon-Nakhon Campus, Sakon Nakhon 47160Thailand
J. Th. Schonewille
Affiliation:
Department of Farm Animal Health, Animal Nutrition Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
W. H. Hendriks
Affiliation:
Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands Department of Farm Animal Health, Animal Nutrition Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
V. Fievez*
Affiliation:
Department of Animal Production, Laboratory for Animal Nutrition and Animal Product Quality (Lanupro), Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
*
†E-mail: [email protected]
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Abstract

Medium-chain fatty acids (MCFA), for example, capric acid (C10:0), myristic (C14:0) and lauric (C12:0) acid, have been suggested to decrease rumen archaeal abundance and protozoal numbers. This study aimed to compare the effect of MCFA, either supplied through krabok (KO) or coconut (CO) oil, on rumen fermentation, protozoal counts and archaeal abundance, as well as their diversity and functional organization. KO contains similar amounts of C12:0 as CO (420 and 458 g/kg FA, respectively), but has a higher proportion of C14:0 (464 v. 205 g/kg FA, respectively). Treatments contained 35 g supplemental fat per kg DM: a control diet with tallow (T); a diet with supplemental CO; and a diet with supplemental KO. A 4th treatment consisted of a diet with similar amounts of MCFA (i.e. C10:0+C12:0+C14:0) from CO and KO. To ensure isolipidic diets, extra tallow was supplied in the latter treatment (KO+T). Eight fistulated bulls (two bulls per treatment), fed a total mixed ration predominantly based on cassava chips, rice straw, tomato pomace, rice bran and soybean meal (1.5% of BW), were used. Both KO and CO increased the rumen volatile fatty acids, in particular propionate and decreased acetate proportions. Protozoal numbers were reduced through the supplementation of an MCFA source (CO, KO and KO+T), with the strongest reduction by KO. Quantitative real-time polymerase chain reaction assays based on archaeal primers showed a decrease in abundance of Archaea when supplementing with KO and KO+T compared with T and CO. The denaturing gradient gel electrophoresis profiles of the rumen archaeal population did not result in a grouping of treatments. Richness indices were calculated from the number of DGGE bands, whereas community organization was assessed from the Pareto–Lorenz eveness curves on the basis of DGGE band intensities. KO supplementation (KO and KO+T treatments) increased richness and evenness within the archaeal community. Further research including methane measurements and productive animals should elucidate whether KO could be used as a dietary methane mitigation strategy.

Keywords

krabok oilcoconut oilrumen protozoaArchaeamicrobial community organization
Type
Nutrition
Information
animal , Volume 7 , Issue 12 , December 2013 , pp. 1950 - 1958
Copyright
Copyright © The Animal Consortium 2013 

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References

Attwood, GT, Altermann, E, Kelly, WJ, Leahy, SC, Zhang, L and Morrison, M 2011. Exploring rumen methanogen genomes to identify targets for methane mitigation strategies. Animal Feed Science and Technology 166–167, 65–75.Google Scholar
Boeckaert, C, Fievez, V, Van Hecke, D, Verstraete, W and Boon, N 2007. Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle. European Journal of Lipid Science and Technology 109, 767–777.CrossRefGoogle Scholar
Boon, N, Top, EM, Verstraete, W and Siciliano, SD 2003. Bioaugmentation as a tool to protect the structure and function of an activated-sludge microbial community against a 3-chloroaniline shock load. Applied and Environmental Microbiology 69, 1511–1520.Google Scholar
Boon, N, Pycke, BFG, Marzorati, M and Hammes, F 2011. Nutrient gradients in a granular activated carbon biofilter drives bacterial community organization and dynamics. Water Research 45, 6355–6361.Google Scholar
Dohme, F, Machmüller, A, Wasserfallen, A and Kreuzer, M 2000. Comparative efficiency of various fats rich in medium-chain fatty acids to suppress ruminal methanogenesis as measured with RUSITEC. Canadian Journal of Animal Science 80, 473–482.CrossRefGoogle Scholar
Dohme, F, Machmüller, A, Wasserfallen, A and Kreuzer, M 2001. Ruminal methanogenesis as influenced by indifidual acids supplemented to complete ruminant diets. Letters in Applied Microbiology 32, 47–51.CrossRefGoogle ScholarPubMed
Dohme, F, Machmüller, A, Estermann, BL, Pfister, P, Wasserfallen, A and Kreuzer, M 1999. The role of the rumen ciliate protozoa for methane suppression caused by coconut oil. Letters in Applied Microbiology 29, 187–192.CrossRefGoogle Scholar
Finlay, BJ, Esteban, G, Clarke, KJ, Williams, AG, Embley, TM and Hirt, RP 1994. Some rumen ciliates have endosymbiotic methanogens. FEMS Microbiology Letters 117, 157–162.CrossRefGoogle ScholarPubMed
Galyean, M 1989. Laboratory procedure in Animal Nutrition Research. Department of Animal and Range Sciences, New Mexico State University, USA.Google Scholar
Goel, G, Arvidsson, K, Vlaeminck, B, Bruggeman, G, Deschepper, K and Fievez, V 2009. Effects of capric acid on rumen methanogenesis and biohydrogenation of linoleic and α-linolenic acid. Animal 3, 810–816.CrossRefGoogle ScholarPubMed
Hristov, AN, Callaway, TR, Lee, C and Dowd, SE 2012. Rumen bacterial, archaeal, and fungal diversity of dairy cows in response to ingestion of lauric or myristic acid. Journal of Animal Science 90, 4449–4457.CrossRefGoogle ScholarPubMed
Hristov, AN, Vander Pol, M, Agle, M, Zaman, S, Schneider, C, Ndegwa, P, Vaddella, VK, Johnson, K, Shingfield, KJ and Karnati, SKR 2009. Effect of lauric acid and coconut oil on ruminal fermentation, digestion, ammonia losses from manure and milk fatty acid composition in lactating cows. Journal of Dairy Science 92, 5561–5582.Google Scholar
Janssen, PH 2010. Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics. Animal Feed Science and Technology 160, 1–22.Google Scholar
Klevenhusen, F, Meile, L, Kreuzer, M and Soliva, CR 2011. Effects of monolaurin on ruminal methanogens and selected bacterial species from cattle, as determined with the rumen simulation technique. Anaerobe 17, 232–238.Google Scholar
Kongmun, P, Wanapat, M, Pakdee, P, Navanukraw, C and Yu, Z 2011. Manipulation of rumen fermentation and ecology of swamp buffalo by coconut oil and garlic powder supplementation. Livestock Science 135, 84–92.Google Scholar
Machmüller, A and Kreuzer, M 1999. Methane suppression by coconut oil and associated effects on nutrient and energy balance in sheep. Canadian Journal of Animal Science 79, 65–72.CrossRefGoogle Scholar
Machmüller, A, Ossowski, DA and Kreuzer, M 2000. Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Animal Feed Science and Technology 85, 41–60.Google Scholar
Machmüller, A, Soliva, CR and Kreuzer, M 2003a. Effect of coconut oil and defaunation treatment on methanogenesis in sheep. Reproduction, Nutrition, Development 43, 41–55.CrossRefGoogle ScholarPubMed
Machmüller, A, Soliva, CR and Kreuzer, M 2003b. Methane-suppressing effect of myristic acid in sheep as affected by dietary calcium and forage proportion. British Journal of Nutrition 90, 529–540.CrossRefGoogle ScholarPubMed
Marzorati, M, Wittebolle, L, Boon, N, Daffonchio, D and Verstraete, W 2008. How to get more out of molecular fingerprints: practical tools for microbial ecology. Environmental Microbiology 10, 1571–1581.CrossRefGoogle ScholarPubMed
Mertens, B, Boon, N and Verstraete, W 2005. Stereo specific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities. Environmental Microbiology 7, 660–669.Google Scholar
Morgavi, DP, Martin, C, Jouany, JP and Ranilla, MJ 2012. Rumen protozoa and methanogenesis: not a simple cause-effect relationship. British Journal of Nutrition 107, 388–397.Google Scholar
Panayakaew, P, Goel, G, Lourenço, M, Yuangklang, C and Fievez, V 2013. Medium-chain fatty acids from coconut or krabok oil inhibit in vitro rumen methanogenesis and conversion of non-conjugated dienoic biohydrogenation intermediates. Animal Feed Science and Technology 180, 18–25.Google Scholar
Patra, AK and Yu, Z 2013. Effects of coconut and fish oils on ruminal methanogenesis, fermentation, and abundance and diversity of microbial populations in vitro. Journal of Dairy Science 96, 1–11.CrossRefGoogle ScholarPubMed
Patra, AK, Stiverson, J and Yu, Z 2012. Effects of quillaja and yucca saponins on communities and select populations of rumen bacteria and archaea, and fermentation in vitro. Journal of Applied Microbiology 113, 1329–1340.Google Scholar
Pilajun, R and Wanapat, M 2011. Methane production and methanogen population in rumen liquor of swamp buffalo as influenced by coconut oil and mangosteen peel powder supplementation. Journal of Animal and Verterinary Advances 10, 2523–2527.Google Scholar
Popova, M, Martin, C, Eugene, M, Mialon, MM, Doreau, M and Morgavi, DP 2011. Effect of fibre- and starch-rich finishing diets on methanogenic Archaea diversity and activity in the rumen of feedlot bulls. Animal Feed Science and Technology 166–167, 113–121.CrossRefGoogle Scholar
Reveneau, C, Karnati, SKR, Oelker, ER and Firkins, JL 2012. Interaction of unsaturated fat or coconut oil with monensin in lactating dairy cows fed 12 times daily. I. Protozoal abundance, nutrient digestibility, and microbial protein flow to the omasum. Journal of Dairy Science 95, 2046–2060.CrossRefGoogle ScholarPubMed
Soliva, CR, Hindrichsen, IK, Meile, L, Kreuzer, M and Machmüller, A 2003. Effects of mixtures of lauric and myristic acid on rumen methanogens and methanogenesis in vitro. Letters Applied Microbiology 37, 35–39.Google Scholar
Soliva, CR, Meile, L, Hindrichsen, IK, Kreuzer, M and Machmüller, A 2004. Myristic acid supports the immediate inhibitory effect of lauric acid on ruminal methanogens and methane release. Anaerobe 10, 269–276.Google Scholar
Van Ranst, G, Fievez, V, Vandewalle, M, Van Waes, C, De Riek, J and Van Bockstaele, E 2010. Influence of damaging and wilting red clover on lipid metabolism during ensiling and in vitro rumen incubation. Animal 4, 1528–1540.Google Scholar
Wittebolle, L, Marzorati, M, Clement, L, Balloi, A, Daffonchio, D, Heylen, K, De Vos, P, Verstraete, W and Boon, N 2009. Initial community eveness favours functionality under selective stress. Nature 458, 623–626.CrossRefGoogle Scholar
Yu, Z and Morrison, M 2004. Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 70, 4800–4806.Google Scholar
Yu, Z, García-González, R, Schanbacher, FL and Morrison, M 2008. Evaluations of different hypervariable regions of archaeal 16S rRNA genes in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 74, 889–893.CrossRefGoogle ScholarPubMed