Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-31T15:59:42.992Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of leptin gene polymorphism on the breeding value of milk production traits in Iranian Holstein

Published online by Cambridge University Press:  01 July 2008

M. Sadeghi ,
M. Moradi Shahr Babak ,
G. Rahimi  and
A. Nejati Javaremi
M. Sadeghi*
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Tehran, Karaj, Iran
M. Moradi Shahr Babak
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Tehran, Karaj, Iran
G. Rahimi
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Mazandaran, Sari, Iran
A. Nejati Javaremi
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Tehran, Karaj, Iran
*
E-mail: [email protected]
Get access

Abstract

New molecular techniques focused on genome analysis, open new possibilities for more accurate evaluation of economiclly important traits in farm animals. Milk production traits are typical quantitative characteristics controlled by a number of genes. Mutations in their sequences may alter animal performance as well as their breeding values. In this study, we investigated the effect of Kpn2I restriction fragment length polymorphisms in the leptin gene, on bull breeding values for milk yield, fat, and protein yield, and their percentage. In order to test for an association between the leptin single-nucleotide polymorphism in exon 2 and milk productivity, we genotyped 134 Iranian Holstein bulls. Breeding values for milk-related traits (milk yield, fat, and protein yield and percentage) were estimated using the BLUP based on an animal model. The effect of the genotypes of Kpn2I polymorphism on the breeding values for milk-related traits was examined using least square methods. The T allele frequency was 0.425. Genotypes were distributed according to the Hardy-Weinberg equilibrium. Bulls with TT genotype had higher milk, fat and protein yield compared with TC and CC bulls (P < 0.05). Bulls with CC genotype had higher protein percentage compared with TT and TC bulls (P < 0.05). The association between leptin polymorphism with milk production traits suggests that this marker may be useful for selection based on molecular information.

Keywords

leptin genepolymorphismmilk productionholstein
Type
Full Paper
Information
animal , Volume 2 , Issue 7 , July 2008 , pp. 999 - 1002
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Block, SS, Butler, WR, Ehrhardt, RA, Bell, AW, Van Amburgh, ME, Boisclair, YR 2001. Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. Journal of Endocrinology 171, 339–348.Google ScholarPubMed
Buchanan, FC, Fitzsimmons, CJ, Van Kessel, AG, Thue, TD, Winkelman-Sim, DC, Schmutz, SM 2002. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genetics, Selection, Evolution 34, 105–116.CrossRefGoogle ScholarPubMed
Buchanan, FC, Van Kessel, AG, Waldner, C, Christensen, DA, Laarveld, B, Schmutz, M 2003. Hot topic: An association between a leptin single nucleotide Polymorphism and milk and protein yield. Journal of Dairy Science 86, 3164–3166.CrossRefGoogle ScholarPubMed
Choudhary, V, Kumar, P, Bhattacharya, TK, Bhushan, B, Sharma, A 2005. DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. Genetics and Molecular Biology 28, 740–742.CrossRefGoogle Scholar
Falconer, DS, Mackay, TFC 1996. Introduction to Quantitative Genetics, 4th edition. Addison Wesley Longman Ltd., Essex, UK.Google Scholar
Haegeman, A, Van Zeveren, A, Peelman, LJ 2000. New mutation in exon 2 of bovine leptin gene. Animal Genetics 31, 79.CrossRefGoogle ScholarPubMed
Halaas, JL, Gajiwala, KS, Maffei, M, Cohen, SL, Chait, BT, Rabinowitz, D, Lallone, RL, Burley, SK, Friedman, JM 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543–546.CrossRefGoogle ScholarPubMed
Hamann, A, Matthaei, S 1996. Regulation of energy balance by leptin. Experimental and Clinical Endocrinology and Diabetes 104, 293–300.CrossRefGoogle ScholarPubMed
Kononoff, PJ, Deobald, HM, Stewart, EL, Laycock, AD, Marquess, FLS 2005. The effect of a leptin single nucleotide polymorphism on quality grade, yield grade, and carcass weight of beef cattle. Journal of Animal Science 83, 927–932.Google ScholarPubMed
Lagonigro, R, Wiener, P, Pilla, F, Woolliams, JA, Williams, JL 2003. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Animal Genetics 34, 371–374.CrossRefGoogle ScholarPubMed
Madeja, Z, Adamowicz, T, Chmurzynska, A, Jankowski, T, Melonek, J, Switonski, M, Strabel, T 2004. Effect of Leptin gene polymorphisms on breeding value for milk production traits. Journal of Dairy Science 87, 3925–3927.CrossRefGoogle ScholarPubMed
Magni, P, Motta, M, Martini, L 2000. Leptin: A possible link between food intake, energy expenditure, and reproductive function. Regulatory Peptides 92, 51–56.Google ScholarPubMed
Pomp, D, Zou, T, Clutter, AC, Barendse, W 1997. Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. Journal of Animal Science 75, 1427.CrossRefGoogle ScholarPubMed
Rock, FL, Altman, SW, van Heek, M, Kastelein, RA, Bazan, JF 1996. The leptin haemopoietic cytokine fold is stabilized by an intrachain disulfide bond. Hormone and Metabolic Research 28, 649–652.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute 2002. SAS user’s guide, version 9.00. SAS Institute Inc., Cary, NC, USA.Google Scholar
Wang T, Fernando RL and Kachman SD (2002) MATVEC user’s guide, version 1.03. Retrieved 15 July 2002 from http://statistics.unl.edu/faculty/steve/software/matvec/Google Scholar
Zadworny, D, Kühnlein, U 1990. The identification of the kappa-casein genotype in Holstein dairy cattle using the polymerase chain reaction. Theoretical and Applied Genetics 80, 631–634.CrossRefGoogle ScholarPubMed