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Effect of forage on the content of phyto-oestrogens in bovine milk

Published online by Cambridge University Press:  01 April 2009

C. Andersen ,
M. R. Weisbjerg ,
J. Hansen-Møller  and
K. Sejrsen
C. Andersen
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
M. R. Weisbjerg
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
J. Hansen-Møller
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
K. Sejrsen*
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
*
E-mail: [email protected]
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Abstract

Phyto-oestrogens are believed to have a range of beneficial effects on predominant Western diseases. A few studies on phyto-oestrogens in milk exist and show that the composition can be affected by feeding. Therefore, the aim was to study how feeding of lucerne and grass/clover silages (GCSs) affects the concentration of phyto-oestrogens in bovine milk. Sixteen Danish Holstein cows were assigned to a 4 × 4 latin square design with four cows per treatment per period of 3 weeks. The four treatment diets were lucerne silage (LS), 2/3 lucerne silage and 1/3 maize silage (2/3LS), 1/3 lucerne silage and 2/3 maize silage (1/3LS) and GCS. Milk was collected at the end of each period and feed samples on day 6, 13 and 20 in each experimental period. Milk and pooled feed samples were analysed for the concentration of isoflavones, coumestans and lignans. The content of isoflavones was higher and the content of coumestrol lower in the GCS diet than in LS, 2/3LS and 1/3LS diets. For the LS, 2/3LS and 1/3LS diets, the concentration of coumestrol and secoisolariciresinol increased with the proportion of lucerne while the concentration of isoflavones was similar across the diets. The concentrations of the formononetin, daidzein and equol in the milk were significantly higher for the GCS diet than for the LS, 2/3LS and 1/3LS diets. In particular, the concentration of equol was 62–291 times higher for GCS. The concentration of coumestrol was significantly lower for the GCS diet compared to the LS, 2/3LS and 1/3LS diets. No pattern for the concentration of lignans was observed. In conclusion, a high concentration of isoflavones, particularly equol, and a low concentration of coumestrol were measured in the milk from the GCS diet. In contrast, a low concentration of isoflavones and a high concentration of coumestrol were measured in the milk from the LS, 2/3LS and 1/3LS diets. However, the concentration of phyto-oestrogens in bovine milk is low compared to food sources rich in phyto-oestrogens but the high concentration of equol could possibly be of therapeutic importance.

Keywords

phyto-oestrogensbovine milklucerne silagegrass/clover silagemaize silage
Type
Full Paper
Information
animal , Volume 3 , Issue 4 , April 2009 , pp. 617 - 622
Copyright
Copyright © The Animal Consortium 2008

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References

Adams, NR 1989. Phytoestrogens. In Toxicants of plant origin – phenolics (ed. RP Cheeke), pp. 23–51. CRC Press Inc., Boca Raton, FL, USA.Google Scholar
Adlercreutz, H 2002. Phyto-oestrogens and cancer. The Lancet Oncology 3, 364–373.CrossRefGoogle ScholarPubMed
Antignac, JP, Cariou, R, Le Bizec, B, Andre, F 2004. New data regarding phytoestrogens content in bovine milk. Food Chemistry 87, 275–281.CrossRefGoogle Scholar
Arora, A, Nair, MG, Strasburg, GM 1998. Antioxidant activities of isoflavones and their biological metabolites in a liposomal system. Archives of Biochemistry and Biophysics 356, 133–141.CrossRefGoogle Scholar
Committee on Toxicity of Chemicals in Food 2003. COT report – phytoestrogens and health. Food Standards Agency, London.Google Scholar
Duncan, AM, Phipps, WR, Kurzer, MS 2003. Phyto-oestrogens. Best Practice and Research. Clinical Endocrinology and Metabolism 17, 253–271.CrossRefGoogle ScholarPubMed
Hansen, B 1989. Determination of nitrogen as elementary N, an alternative to Kjeldahl. Acta Agriculturae Scandinavica Section A-Animal Science 39, 113–118.Google Scholar
Hoikkala, A, Mustonen, E, Saastmoinen, I, Jokela, T, Teponen, J, Saloniemi, H, Wähälä, K 2007. High levels of equol in skimmed Finnish cow milk. Molecular Nutrition & Food Research 51, 782–786.CrossRefGoogle ScholarPubMed
King, RA, Mano, MM, Head, RJ 1998. Assessment of isoflavonoid concentrations in Australian bovine milk samples. Journal of Dairy Research 65, 479–489.CrossRefGoogle ScholarPubMed
Kurzer, MS, Xu, X 1997. Dietary phytoestrogens. Annual Review of Nutrition 17, 353–381.CrossRefGoogle ScholarPubMed
Lampe, JW, Karr, SC, Hutchins, AM, Slavin, JL 1998. Urinary equol excretion with a soy challenge: influence of habitual diet. Experimental Biology and Medicine 217, 335–339.CrossRefGoogle ScholarPubMed
Lund, TD, Munson, DJ, Haldy, ME, Setchell, KD, Lephart, ED, Handa, RJ 2004. Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback. Biology of Reproduction 70, 1188–1195.CrossRefGoogle ScholarPubMed
Magee, PJ, Rowland, IR 2004. Phyto-oestrogens, their mechanism of action: current evidence for a role in breast and prostate cancer. British Journal of Nutrition 91, 513–531.CrossRefGoogle ScholarPubMed
Mertens, DR 2002. Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International 85, 1217–1240.Google ScholarPubMed
Purup, S, Hansen-Møller, J, Sejrsen, K, Christensen, LP, Lykkesfeldt, AE, Leffers, HSkakkebæk, NE 2005. Increased phytoestrogen content in organic milk and the biological importance. Newsletter from Danish Research Centre for Organic Farming, no. 2. Danish Research Centre for Organic Farming, Tjele, Denmark.Google Scholar
Rowland, IR, Wiseman, H, Sanders, TA, Adlercreutz, H, Bowey, EA 2000. Interindividual variation in metabolism of soy isoflavones and lignans: influence of habitual diet on equol production by the gut microflora. Nutrition and Cancer 36, 27–32.CrossRefGoogle ScholarPubMed
Saloniemi, H, Wahala, K, Nykanen-Kurki, P, Kallela, K, Saastamoinen, I 1995. Phytoestrogen content and estrogenic effect of legume fodder. Experimental Biology and Medicine 208, 13–17.CrossRefGoogle ScholarPubMed
Setchell, KDR, Brown, NM, Lydeking-Olsen, E 2002. The clinical importance of the metabolite equol – a clue to the effectiveness of Soy and its isoflavones. Journal of Nutrition 132, 3577–3584.CrossRefGoogle Scholar
Statistical Analysis Systems Institute (SAS) 1999. SAS/STAT software. Release 8.02. SAS Institute Inc., Cary, NC, USA.Google Scholar
Steinshamn, H, Purup, S, Thuen, E, Hansen-Møller, J 2008. Effects of clover silages and concentrate supplementation on the content of phytoestrogens in dairy cow milk. Journal of Dairy Science 91, 2715–2725.CrossRefGoogle ScholarPubMed
Stoldt, OW 1952. Vorschlag zur vereinheitlichung der fettbestimmung in lebensmitteln. Fette, Seifen, Anstrichsmittel 54, 206–207.CrossRefGoogle Scholar
Thompson, LU, Robb, P, Serraino, M, Cheung, F 1991. Mammalian lignan production from various foods. Nutrition and Cancer 16, 43–52.CrossRefGoogle ScholarPubMed
Wu, Q, Wang, M, Simon, JE 2003. Determination of isoflavones in red clover and related species by high-performance liquid chromatography combined with ultraviolet and mass spectrometric detection. Journal of Chromatography A 1016, 195–209.CrossRefGoogle ScholarPubMed