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Effect of exogenous insulin and fasting on growth hormone receptor and IGF-I expression in the pre-ovulatory follicle of ewes

Published online by Cambridge University Press:  31 March 2011

A. Schneider ,
L. F. M. Pfeifer ,
J. W. da Silva Neto ,
L. T. Hax ,
M. M. Antunes ,
F. A. B. Del Pino ,
G. R. Paludo  and
M. N. Corrêa
A. Schneider*
Affiliation:
Biotechnology Center, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
L. F. M. Pfeifer
Affiliation:
Department of Veterinary Clinics, Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
J. W. da Silva Neto
Affiliation:
Department of Veterinary Clinics, Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
L. T. Hax
Affiliation:
Department of Veterinary Clinics, Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
M. M. Antunes
Affiliation:
Department of Veterinary Clinics, Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
F. A. B. Del Pino
Affiliation:
Department of Biochemistry, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
G. R. Paludo
Affiliation:
Department of Pathology, Veterinary College, University of Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, CEP 70910-970, Brasília, DF, Brazil
M. N. Corrêa
Affiliation:
Department of Veterinary Clinics, Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Veterinary College, Federal University of Pelotas, Campus Universitário, CEP 96010-900, Pelotas, RS, Brazil
*
†E-mail: [email protected]
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Abstract

The aim of this study was to investigate the effect of fasting and exogenous insulin administration on the expression of growth hormone receptor (GHR) and IGF-I mRNA in the pre-ovulatory follicle of ewes. Fifteen ewes received an intravaginal progesterone releasing device that was removed 6 days later (day of removal = day 0). On day −2, the ewes were divided into three groups: (i) fasting group (n = 5) that was fasted from day −2 to day 2; (ii) control group (n = 5) that received a maintenance diet; and (iii) insulin group (n = 5) that received insulin injections (0.25 IU/kg) every 12 h from day −2 to day 2 under the same diet as the control group. Follicular samples were obtained on day 2. Fasting increased plasma non-esterified fatty acids concentrations from day −1 to day 2 (P < 0.001). There was no difference (P > 0.05) in the number of follicles, although there was a tendency for an increase in the pre-ovulatory follicle diameter for the insulin group in comparison to the control group (P = 0.12). Thecal GHR mRNA expression was very low and was considered insignificant. Moreover, granulosa cells GHR mRNA expression increased (P < 0.05) in the insulin group. Expression of IGF-I mRNA was not different among groups in both tissues. In conclusion, insulin administration increases GHR mRNA but not IGF-I mRNA expression in granulosa cells of the pre-ovulatory follicle. However, fasting did not change the pattern of GHR/IGF-I mRNA expression in the pre-ovulatory follicle.

Keywords

GHRIGF-ImRNAovarysheep
Type
Full Paper
Information
animal , Volume 5 , Issue 9 , 05 August 2011 , pp. 1420 - 1425
Copyright
Copyright © The Animal Consortium 2011

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