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The effect of equol injection in ovo on posthatch growth, meat quality and antioxidation in broilers

Published online by Cambridge University Press:  17 September 2010

X. J. Wei ,
Y. D. Ni ,
L. Z. Lu ,
R. Grossmann  and
R. Q. Zhao
X. J. Wei
Affiliation:
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China
Y. D. Ni*
Affiliation:
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China
L. Z. Lu
Affiliation:
Animal Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People’s Republic of China
R. Grossmann
Affiliation:
Department of Functional Genomics & Bioregulation, Institute of Animal Science Mariensee, 31535 Neustadt, Germany
R. Q. Zhao
Affiliation:
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China
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Abstract

In order to investigate the long-term effects of equol (Eq) on growth and meat quality in broilers, 0 μg (control, Con), 20 μg (low dose, L) and 100 μg (high dose, H) Eq, respectively, were injected into fertile eggs (146 eggs per group) on 7 days of embryos. After hatch, chickens were fed under the same conditions and slaughtered at 49 days of age for sample collection and analysis. The results showed that body weight and composition were marginally affected by Eq administration (P > 0.05). Compared with their male counterparts, the meat quality of female broilers was affected greatly after Eq administration. The redness (a*) of meat color in the L and H groups of female broilers was significantly decreased by 24.10% and 21.50% (P < 0.01), respectively; cooking loss decreased by 12.11% and 16.82%, respectively, in the L and H groups (P < 0.01); 24 h and 48 h drip loss was significantly decreased by 60.27% and 45.72% (P < 0.05), respectively, in the H group. However, for male broilers, only cooking loss was significantly decreased by high dosage of Eq treatment (P < 0.05). The antioxidative status was analyzed for discovering further the mechanism behind the improvement of the water-holding capacity caused by Eq in female broilers. The activity of glutathione peroxidase (GSHPx) in plasma was greatly increased by 15.94% in the L group (P < 0.01), whereas the total superoxide dismutase activity (T-SOD) and the content of malondialdehyde in plasma were not changed (P > 0.05). The T-SOD activity in the breast muscle of the L and H groups were significantly improved by 23.14% and 18.82% (P < 0.05), respectively. GSHPx in the breast muscle of the H group showed a tendency to increase (P = 0.06 < 0.1). These results indicate that Eq injection in ovo does not affect the growth of broilers, but significantly improves the water-holding capacity of the muscle, especially in female broilers, which is related to the improvement of antioxidative status.

Keywords

equolin ovo injectionmeat qualityantioxidationbroiler
Type
Full Paper
Information
animal , Volume 5 , Issue 2 , February 2011 , pp. 320 - 327
Copyright
Copyright © The Animal Consortium 2010

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References

Atkinson, C, Frankenfeld, CL, Lampe, JW 2005. Gut bacterial metabolism of the soy isoflavone daidzein: exploring the relevance to human health. Experimental Biology and Medicine 230, 155–170.Google Scholar
Axelson, M, Kirk, DN, Farrant, RD, Cooley, G, Lawson, AM, Setchell, KD 1982. The identification of the weak oestrogen equol [7-hydroxy-3-(4’-hydroxyphenyl) chroman] in human urine. Biochemical Journal 201, 353–357.Google Scholar
Bhathena, SJ, Velasquez, MT 2002. Beneficial role of dietary phytoestrogens in obesity and diabetes. American Journal of Clinical Nutrition 76, 1191–1201.CrossRefGoogle ScholarPubMed
Cassidy, A 2003. Potential risks and benefits of phytoestrogen-rich diets. International Journal for Vitamin and Nutrition Research 73, 120–126.Google Scholar
Chung, JE, Kim, SY, Jo, HH, Hwang, SJ, Chae, B, Kwon, DJ, Lew, YO, Lim, YT, Kim, JH, Kim, EJ, Kim, JH, Kim, MR 2008. Antioxidant effects of equol on bovine aortic endothelial cells. Biochemical and Biophysical Research Communications 375, 420–424.CrossRefGoogle ScholarPubMed
Cos, P, De Bruyne, T, Apers, S, Vanden Berghe, D, Pieters, L, Vlietinck, AJ 2003. Phytoestrogens: recent developments. Planta Medica 69, 589–599.Google ScholarPubMed
Dwiecki, K, Neunert, G, Polewski, P, Polewski, K 2009. Antioxidant activity of daidzein, a natural antioxidant, and its spectroscopic properties in organic solvents and phosphatidylcholine liposomes. Journal of Photochemistry and Photobiology – B: Biology 96, 242–248.Google Scholar
Holm, L, Blomqvist, A, Brandt, I, Brunström, B, Ridderstråle, Y, Berg, C 2006. Embryonic exposure to o, p-DDT causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen. Environmental Toxicology and Chemistry 25, 2787–2793.CrossRefGoogle Scholar
Hwang, CS, Kwak, HS, Lim, HJ, Lee, SH, Kang, YS, Choe, TB, Hur, HG, Han, KO 2006. Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration. The Journal of Steroid Biochemistry and Molecular Biology 101, 246–253.Google Scholar
Jackman, KA, Woodman, OL, Chrissobolis, S, Sobey, CG 2007a. Vasorelaxant and antioxidant activity of the isoflavone metabolite equol in carotid and cerebral arteries. Brain Research 1141, 99–107.Google Scholar
Jackman, KA, Woodman, OL, Sobey, CG 2007b. Isoflavones, equol and cardiovascular disease: pharmacological and therapeutic insights. Current Medicinal Chemistry 14, 2824–2830.Google Scholar
Jiang, ZY, Jiang, SQ, Lin, YC, Xi, PB, Yu, DQ, Wu, TX 2007. Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers. Poultry Science 86, 1356–1362.CrossRefGoogle ScholarPubMed
Liu, J, Chang, SK, Wiesenborn, D 2005. Antioxidant properties of soybean isoflavone extract and tofu in vitro and in vivo. Journal of Agricultural and Food Chemistry 53, 2333–2340.Google Scholar
Liu, H, Zhang, C, Zeng, W 2006. Estrogenic and antioxidant effects of a phytoestrogen daidzein on ovarian germ cells in embryonic chickens. Domestic Animal Endocrinology 31, 258–268.Google Scholar
Liu, GT, Zheng, YL, Chen, WH, Chen, J, Han, ZK 1999. Effect of daidzein fed to pregnant sows on milk production and the levels of hormones in colostrum. Journal of Nanjing Agricultural University 22, 69–72.Google Scholar
Marrian, GF, Haslewood, GA 1932. Equol, a new inactive phenol isolated from the ketohydroxyoestrin fraction of mares’ urine. Biochemical Journal 26, 1227–1232.Google Scholar
Matthies, A, Clavel, T, Gütschow, M, Engst, W, Haller, D, Blaut, M, Braune, A 2008. Conversion of daidzein and genistein by an anaerobic bacterium newly isolated from the mouse intestine. Applied and Environmental Microbiology 74, 4847–4852.Google Scholar
Minamida, K, Tanaka, M, Abe, A, Sone, T, Tomita, F, Hara, H, Asano, K 2006. Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine. Journal of Bioscience and Bioengineering 102, 247–250.Google Scholar
Mitchell, JH, Gardner, PT, McPhail, DB, Morrice, PC, Collins, AR, Duthie, GG 1998. Antioxidant efficacy of phytoestrogens in chemical and biological model systems. Archives of Biochemistry and Biophysics 360, 142–148.CrossRefGoogle ScholarPubMed
Muthyala, RS, Ju, YH, Sheng, S, Williams, LD, Doerge, DR, Katzenellenbogen, BS, Helferich, WG, Katzenellenbogen, JA 2004. Equol, a natural estrogenic metabolite from soy isoflavones: convenient preparation and resolution of R- and S-equols and their differing binding and biological activity through estrogen receptors alpha and beta. Bioorganic & Medicinal Chemistry 12, 1559–1567.CrossRefGoogle ScholarPubMed
Ni, YD, Hong, WJ, Ren, LZ, Hu, Y, Dai, J, Rao, KQ, Chen, J, Zhao, RQ 2007. Effect of dietary daidzein on egg quality and meat quality of the offspring of broiler breed hens during the late period of laying cycle. Acta Veterinaria et Zootechnica Sinica 38, 1188–1194.Google Scholar
Payne, RL, Bidner, TD, Southern, LL, Geaghan, JP 2001a. Effects of dietary soy isoflavones on growth, carcass traits, and meat quality in growing-finishing pigs. Journal of Animal Science 79, 1230–1239.CrossRefGoogle ScholarPubMed
Payne, RL, Bidner, TD, Southern, LL, Mcmillin, KW 2001b. Dietary effects of soy isoflavones on growth and carcass traits of commercial broilers. Poultry Science 80, 1201–1207.Google Scholar
Price, KR, Fenwick, GR 1985. Naturally occurring oestrogens in foods – a review. Food Additives and Contaminants 2, 73–106.CrossRefGoogle ScholarPubMed
Rehfeldt, C, Adamovic, I, Kuhn, G 2007. Effects of dietary daidzein supplementation of pregnant sows on carcass and meat quality and skeletal muscle cellularity of the progeny. Meat Science 75, 103–111.CrossRefGoogle ScholarPubMed
Ren, MQ, Kuhn, G, Wegner, J, Nürnberg, G, Chen, J, Ender, K 2001. Feeding daidzein to late pregnant sows influences the estrogen receptor beta and type 1 insulin-like growth factor receptor mRNA expression in newborn piglets. Journal of Endocrinology 170, 129–135.Google Scholar
Saitoh, S, Sato, T, Harada, H, Takita, T 2001. Transfer of soy isoflavone into the egg yolk of chickens. Bioscience, Biotechnology and Biochemistry 65, 2220–2225.Google Scholar
Yu, LH, Lee, ES, Jeong, JY, Paik, HD, Choi, JH, Kim, CJ 2005. Effects of thawing temperature on the physicochemical properties of pre-rigor frozen chicken breast and leg muscles. Meat Science 71, 375–382.Google Scholar