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Effect of dietary sources of n-3 fatty acids on pig performance and technological, nutritional and sensory qualities of pork

Published online by Cambridge University Press:  20 November 2017

A. de Tonnac  and
J. Mourot
A. de Tonnac
Affiliation:
Agrocampus Ouest, INRA, F-35590 Saint-Gilles, France
J. Mourot*
Affiliation:
Agrocampus Ouest, INRA, F-35590 Saint-Gilles, France
*
†E-mail: [email protected]
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Abstract

In France, animal products (dairy products, meat and eggs) are the main source of n-3 polyunsaturated fatty acids (PUFA) in the human diet; however, many individuals do not consume enough of this nutrient. The objective of this study was to increase n-3 PUFA precursor and derivative contents in tissues and test how they influence technological and sensory qualities of meat without negatively affecting growth performances of pigs. A total of 60 male pigs [(Large White×Landrace)×Pietrain] were assigned according to their initial liveweight (50.7±2.7 kg) to five experimental groups corresponding to five different diets that they received from 14 to 22 weeks of age. Dietary lipid supplements were composed of soybean and palm oil (SP), dehulled and extruded linseed (EL-), docosahexaenoic acid (DHA)-rich microalgae (MAG) or a mixture of linseed and microalgae at 75%/25% (3EL-/MAG) and 50%/50% (EL-/MAG), respectively. Diet did not influence growth performances of pigs or the technological quality of the meat. The n-3 PUFA content in the longissimus dorsi muscle, subcutaneous backfat (SCB) and liver increased with a dietary supply of linseed and microalgae and corresponded to circulating fatty acids (FA). The amount of malondialdehyde, representative of FA lipid peroxidation measured in SCB, increased significantly with the supply of microalgae, meaning that PUFA from the microalgae included in the diet increased the meat’s susceptibility to oxidation. The MAG diet scored highest for ‘abnormal’ flavor, similar to that of fish or organ meat, but the n-3 PUFA-rich diet had no effect on other sensory characteristics. Results of this study indicate benefits of enriching animal feed with n-3 PUFA, but the inclusion of long-chain n-3 PUFA such as DHA must be limited to avoid oxidation susceptibility and development of an off-odor.

Keywords

pigmeatfatty acidslinseedmicroalgae
Type
Research Article
Information
animal , Volume 12 , Issue 7 , July 2018 , pp. 1527 - 1535
Copyright
© The Animal Consortium 2017 

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References

Association Française de Normalisation (AFNOR) 1982. Animal feed. Determination of water content. In NF V18-109, 5.Google Scholar
Association Française de Normalisation (AFNOR) 2010. Sensorial analysis – general guidelines for designing premises for analysis. In NF V09-105, 5.Google Scholar
Baéza, E, Chartrin, P, Lessire, M, Meteau, K, Chesneau, G, Guillevic, M and Mourot, J 2015. Is it possible to increase n-3 fatty acid content of meat without affecting its technological and/or sensory quality and the growing performance of chickens ? British Poultry Science 56, 748–754.Google Scholar
Barclay, WR, Meager, KM and Abril, JR 1994. Heterotrophic production of long-chain omega-3 fatty acids utilizing algae and algae-like microorganisms. Journal of Applied Phycology 6, 123–129.CrossRefGoogle Scholar
Bligh, E.G. and Dyer, W.J 1959. A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.Google Scholar
Botsford, LW 1997. The management of fisheries and marine ecosystems. Science 277, 509–515.Google Scholar
Botsoglou, E, Govaris, A, Ambrosiadis, I and Fletouris, D 2012. Lipid and protein oxidation of α-linolenic acid-enriched pork during refrigerated storage as influenced by diet supplementation with olive leaves (Olea europea L.) or α-tocopheryl acetate. Meat Science 92, 525–532.Google Scholar
Brossard, N, Croset, M, Pachiaudi, C, Paul Riots, JP and Tavot, JL 1996. Retroconversion and metabolism of DHA in humans and rats after intake of a single dose of DHA triacylglycerols. American Journal of Clinical Nutrition 64, 577–586.Google Scholar
Buege, JA and Aust, SD 1978. Microsomal lipid peroxidation. Methods of Enzymology 52, 302–310.CrossRefGoogle ScholarPubMed
Corino, C, Magni, S, Pagliarini, E, Rossi, R, Pastorelli, G and Chiesa, LM 2002. Effects of dietary fats on meat quality and sensory characteristics of heavy pig loins. Meat Science 60, 1–8.Google Scholar
Corino, C, Rossi, R, Cannata, S and Ratti, S 2014. Effect of dietary linseed on the nutritional value and quality of pork and pork products: systematic review and meta-analysis. Meat Science 98, 679–688.Google Scholar
Folch, J, Lees, M and Sloane Stanley, GH 1957. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Gronn, M, Christensen, E, Hagve, TA and Christophersen, BO 1991. Peroxisomal retroconversion of docosahexaenoic acid to EPA studied in isolated rat liver cells. Biochimica and Biophysica Acta 1081, 85–91.CrossRefGoogle ScholarPubMed
Guillevic, M, Kouba, M and Mourot, J 2009. Effect of a linseed diet or a sunflower diet on performances, fatty acid composition, lipogenic enzyme activities and stearoyl-CoA-desaturase activity in the pig. Livestock Science 124, 288–294.Google Scholar
Högberg, A, Pickova, J, Andersson, B and Lundströma, K 2003. Fatty acid composition and tocopherol content of muscle in pigs fed organic and conventional feed with different n6/n3 ratios, respectively. Food Chemisry 80, 177–186.CrossRefGoogle Scholar
Honikel, K 1998. Reference methods for the assessment of physical characteristics of meat. Meat Science 49, 447–457.Google Scholar
International Organization for Standardization (ISO) 1998. Animal feeding stuffs, animal products, and faeces or urine – determination of gross calorific value – bomb calorimeter method. In ISO 9831:1998, 23.Google Scholar
International Organization for Standardization (ISO) 2008. Food products – determination of total nitrogen content by combustion according to the Dumas principle and calculation of crude protein content. Part 1: oilseeds and animal feed. In ISO 16634-1:2008, 30.Google Scholar
Kornbrust, DJ and Mavis, RD 1980. Relative susceptibility of microsomes from lung, kidney, brain and testes to lipid peroxidation: correlation with vitamin E content. Lipids 15, 315–322.Google Scholar
Kouba, M, Enser, M, Whittington, FM, Nute, GR and Wood, JD 2003. Effect of a high-linolenic acid diet on lipogenic enzyme activities, fatty acid composition, and meat quality in the growing pig. Journal of Animal Science 81, 1967–1979.Google Scholar
Lavialle, M and Layé, S 2010. Acides gras poly-insaturés (omega 3, omega 6) et fonctionnement du système nerveux central [Polyunsaturated fatty acids (omega 3, omega 6) and brain functions]. Innovations Agronomiques 10, 25–42.Google Scholar
Marriott, NG, Garrett, JE, Sims, MD and Abril, JR 2002. Performance characteristics and fatty acid composition of pigs fed a diet with docosahexaenoic acid. Journal of Muscle Foods 13, 265–277.Google Scholar
Meadus, WJ, Duff, P, Uttaro, B, Aalhus, JL, Rolland, DC, Gibson, LL and Dugan, ME 2010. Production of docosahexaenoic acid (DHA) enriched bacon. Journal of Agricultural Food Chemistry 58, 465–472.Google Scholar
Missotten, J, De Smet, S, Raes, K and Doran, O 2009. Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of Delta5- and Delta6-desaturase in tissues of female piglets. Animal 3, 1196–1204.Google Scholar
Monahan, FJ, Buckley, DJ, Morrissey, PA, Lynch, PB and Gray, JI 1992. Influence of dietary fat and alpha-tocopherol supplementation on lipid oxidation in pork. Meat Science 31, 229–241.Google Scholar
Morrison, WR and Smith, LM 1964. Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron fluoride methanol. Journal of Lipid Research 5, 600–608.CrossRefGoogle Scholar
Mourot, J and Guillevic, M 2015. Effect of introducing hemp oil into feed on the nutritional quality of pig meat. Oilseeds and fats, Crops and Lipids 22, D612.Google Scholar
Mourot, J and Hermier, D 2001. Lipids in monogastric animal meat. Reproduction Nutrition Development 41, 109–118.Google Scholar
Portolesi, R, Powell, BC and Gibson, RA 2007. Competition between 24:5n-3 and ALA for Delta 6 desaturase may limit the accumulation of DHA in HepG2 cell membranes. Journal of Lipid Research 48, 1592–1598.Google Scholar
Sardi, L, Martelli, G, Lambertini, L, Parisini, P and Mordenti, A 2006. Effects of a dietary supplement of DHA-rich marine algae on Italian heavy pig production parameters. Livestock Science 103, 95–103.Google Scholar
Sauvant, D, Perez, JM and Tran, G 2004. Tables of composition and nutritional value of feed materials for livestock. INRA, Rennes, France.Google Scholar
Sobotka, W, Flis, M, Antoszkiewicz, Z, Lipiński, K and Zduńczyk, Z 2012. Effect of oat by-product antioxidants and vitamin E on the oxidative stability of pork from pigs fed diets supplemented with linseed oil. Archives of Animal Nutrition 66, 27–38.Google Scholar
Vossen, E, Claeys, E, Raes, K, Van Mullem, D and De Smet, S 2016. Supra-nutritional levels of α-tocopherol maintain the oxidative stability of n-3 long-chain fatty acid enriched subcutaneous fat and frozen loin, but not of dry fermented Sausage. Journal of the Science of Food and Agriculture 96, 4523–4530.Google Scholar
Vossen, E, Raes, K, Van Mullem, D and De Smet, S 2017. Production of docosahexaenoic acid (DHA) enriched loin and dry cured ham from pigs fed algae: nutritional and sensory quality. European Journal of Lipid Science and Technology 119, 1600144. doi:10.1002/ejlt.201600144.Google Scholar