Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T21:08:25.929Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of dietary lysine on performance and expression of electron transport chain genes in the pectoralis major muscle of broilers

Published online by Cambridge University Press:  21 October 2016

C. O. Brito ,
J. L. L. Dutra ,
T. N. Dias ,
L. T. Barbosa ,
C. S. Nascimento ,
A. P. G. Pinto ,
L. F. T. Albino ,
R. P. M. Fernandes ,
M. S. Macário  and
J. S. Melo
C. O. Brito*
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
J. L. L. Dutra
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
T. N. Dias
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
L. T. Barbosa
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
C. S. Nascimento
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
A. P. G. Pinto
Affiliation:
Department of Animal Science, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil
L. F. T. Albino
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil
R. P. M. Fernandes
Affiliation:
Department of Physiology, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
M. S. Macário
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
J. S. Melo
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil
*
†E-mail: [email protected]
Get access

Abstract

The aim of this study was to evaluate the effect of dietary lysine on performance, protein deposition and respiratory chain gene expression in male broilers. A total of 252 Cobb 500 broilers were distributed, in a completely randomized design, into four treatments with seven replicates of nine birds per experimental unit. Experimental treatments consisted of diets based on corn and soybean meal, with four levels of digestible lysine: 1.016%, 1.099%, 1.182% and 1.265%. The increase in the level of digestible lysine in the diet provided higher weight gains, feed efficiency and body protein deposition. Birds fed the lowest level of dietary lysine (1.016%) showed a lower expression of genes such as NADH dehydrogenase subunit I (ND1), cytochrome b (CYTB) and cytochrome c oxidase subunits I (COX I), II (COX II) and III (COX III), displaying the worst performance and body protein deposition. This demonstrates the relationship existing between the expression of the evaluated genes and the performance responses. In conclusion, results indicate that broilers fed diets with higher levels of digestible lysine have increased messenger RNA expression of some genes coded in the mitochondrial electron transport chain (ND1, CYTB, COX I, COX II and COX III). It may be stated that diets with proper levels of digestible lysine, within the ‘ideal protein’ concept, promote the expression of genes, which increases the mitochondrial energy, thereby fostering body protein deposition and the performance of broilers in the starter phase.

Keywords

amino acidsgenesprotein depositionrespiratory chain
Type
Research Article
Information
animal , Volume 11 , Issue 5 , May 2017 , pp. 778 - 783
Copyright
© The Animal Consortium 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aoyagi, Y, Tasaki, I, Okumura, J and Muramatsu, T 1988. Energy cost of whole-body protein synthesis measured in vivo in chicks. Comparative Biochemistry and Physiology. A, Comparative Physiology 91, 765–776.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists (AOAC) 2000. Official methods of analysis, vol. 2, 17th edition. AOAC, Arlington, VA, USA.Google Scholar
Bottje, WG and Carstens, GE 2009. Association of mitochondrial function and feed efficiency in poultry and livestock species. Journal of Animal Science 87, 48–63.CrossRefGoogle ScholarPubMed
Bottje, WG, Iqbal, M, Tang, ZX, Cawthon, D, Okimoto, R, Wing, T and Cooper, M 2002. Association of mitochondrial function with feed efficiency within a single genetic line of male broilers. Poultry Science 81, 546–555.CrossRefGoogle ScholarPubMed
Bottje, WG, Pumford, NR, Ojano-Dirain, C, Iqbal, M and Lassiter, K 2006. Feed efficiency and mitochondrial function. Poultry Science 85, 8–14.CrossRefGoogle ScholarPubMed
Buttery, PJ and Boorman, KN 1976. Energetic efficiency of amino acid metabolism. In Protein nutrition and metabolism (ed. DJA Cole, KN Boorman, PJ Buttery, D Lewis, RJ Neale and H Swan), pp. 197–206. Publication European Association Animal Production, London, UK.Google Scholar
Buttery, PJ and Lindsay, DB 1980. Protein deposition in animals. Butterworth Publishers, Woburn, MA, USA.Google Scholar
Corzo, A, Mejia, L, McDaniel, CD and Moritz, JS 2012. Interactive effects of feed form and dietary lysine on growth responses of commercial broiler chicks. Journal of Applied Poultry Research 21, 70–78.CrossRefGoogle Scholar
Dozier, WA, Corzo, A, Kidd, MT, Tillman, PB, McMurtry, JP and Branton, SL 2010. Digestible lysine requirements of male broilers from 28 to 42 days of age. Poultry Science 89, 2173–2182.CrossRefGoogle ScholarPubMed
Dozier, WA and Payne, RL 2012. Digestible lysine requirements of female broilers from 1 to 15 days of age. Journal of Applied Poultry Research 21, 348–357.CrossRefGoogle Scholar
Fakhraei, J, Loutfollahian, H, Shivazad, M, Chamani, M and Hoseini, SA 2010. Reevaluation of lysine requirement based on performance responses in broiler breeder hens. African Journal of Agricultural Research 5, 2137–2142.Google Scholar
Fatufe, AA, Timmler, R and Rodehutscord, M 2004. Response to lysine intake in composition of body weight gain and efficiency of lysine utilization of growing male chickens from two genotypes. Poultry Science 83, 1314–1324.CrossRefGoogle ScholarPubMed
Garcia, AR, Batal, AB and Baker, DH 2006. Variations in the digestible lysine requirement of broiler chickens due to sex, performance parameters, rearing environment, and processing yield characteristics. Poultry Science 85, 498–504.CrossRefGoogle ScholarPubMed
Guan, X, Geng, T, Silva, P and Smith, EJ 2007. Mitochondrial DNA sequence and haplotype variation analysis in the chicken (Gallus gallus). Journal of Heredity 98, 723–726.CrossRefGoogle ScholarPubMed
Hocquette, JF, Tesseraud, S, Cassar-Malek, I, Chilliard, Y and Ortigues-Marty, I 2007. Responses to nutrients in farm animals: implications for production and quality. Animal 1297–1313.CrossRefGoogle ScholarPubMed
Iqbal, M, Pumford, NR, Tang, ZX, Lassiter, K, Wing, T, Cooper, M and Bottje, W 2004. Low feed efficient broilers within a single genetic line exhibit higher oxidative stress and protein expression in breast muscle with lower mitochondrial complex activity. Poultry Science 83, 474–484.CrossRefGoogle ScholarPubMed
Iqbal, M, Pumford, NR, Tang, ZX, Lassiter, K, Ojano-Dirain, C, Wing, T, Cooper, M and Bottje, W 2005. Compromised liver mitochondrial function and complex activity in low feed efficient broilers are associated with higher oxidative stress and differential protein expression. Poultry Science 84, 933–941.CrossRefGoogle ScholarPubMed
Jonckheere, AI, Smeitink, JA and Rodenburg, RJ 2012. Mitochondrial ATP synthase: architecture, function and pathology. Journal of Inherited Metabolic Disease 35, 211–225.CrossRefGoogle ScholarPubMed
Kidd, MT, Kerr, BJ, Halpin, KM, McWard, GW and Quarles, CL 1998. Lysine levels in starter and grower-finisher diets affect broiler performance and carcass traits. Journal of Applied Poultry Research 7, 351–358.CrossRefGoogle Scholar
Leclercq, B 1998. Lysine: specific effects of lysine on broiler production: comparison with threonine and valine. Poultry Science 77, 118–123.CrossRefGoogle ScholarPubMed
Leenstra, FR 1986. Effect of age, sex, genotype and environment on fat deposition in broiler chickens – a review. World’s Poultry Science Journal 42, 12–25.CrossRefGoogle Scholar
Mannen, H, Morimoto, M, Oyama, K, Mukai, F and Tsuji, S 2003. Identification of mitochondrial DNA substitutions related to meat quality in Japanese black cattle. Journal of Animal Science 81, 68–73.CrossRefGoogle ScholarPubMed
Nascimento, CS, Barbosa, LT, Brito, CO, Fernandes, RPM, Mann, RS, Pinto, APG, Oliveira, HC, Dodson, MV, Guimarães, SEF and Duarte, MS 2015. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken. PLoS ONE 10, e0127935.CrossRefGoogle ScholarPubMed
Ojano-Dirain, C, Toyomizu, M, Wing, T, Cooper, M and Bottje, WG 2007. Gene expression in breast muscle and duodenum from low and high feed efficient broilers. Poultry Science 86, 372–381.CrossRefGoogle ScholarPubMed
Pearson, AM and Young, RB 1989. Muscle and meat biochemistry. Academic Press, San Diego, CA, USA.Google Scholar
Rostagno, HS, Albino, LFT, Donzele, JL, Gomes, PC, Oliveira, Rd, Lopes, DC, Ferreira, AS, Barreto, S and Euclides, RF 2011. Tabelas brasileiras para aves e suínos. Universidade Federal de Viçosa, DZO, Viçosa, MG, Brasil.Google Scholar
Steibel, JP, Poletto, R, Coussens, PM and Rosa, GJM 2009. A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics 94, 146–152.CrossRefGoogle ScholarPubMed
Tinsley, N, Iqbal, M, Pumford, NR, Lassiter, K, Ojano-Dirain, C, Wing, T and Bottje, W 2010. Investigation of mitochondrial protein expression and oxidation in heart muscle in low and high feed efficient male broilers in a single genetic line. Poultry Science 89, 349–352.CrossRefGoogle Scholar
Urdaneta-Rincon, M and Leeson, S 2004. Muscle (pectoralis major) protein turnover in young broiler chickens fed graded levels of lysine and crude protein. Poultry Science 83, 1897–1903.CrossRefGoogle ScholarPubMed
Wallace, DC 2005. A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: a dawn for evolutionary medicine. Annual Review of Genetics 39, 359–407.CrossRefGoogle ScholarPubMed
Wallace, DC 2007. Why do we still have a maternally inherited mitochondrial DNA? Insights from evolutionary medicine. Annual Review of Biochemistry 76, 781–821.CrossRefGoogle ScholarPubMed
Wang, JW, Chen, W, Kang, XT, Huang, YQ, Tian, YD and Wang, YB 2012. Identification of differentially expressed genes induced by energy restriction using annealing control primer system from the liver and adipose tissues of broilers. Poultry Science 91, 972–978.CrossRefGoogle ScholarPubMed
Watson, JD, Baker, TA, Bell, SP, Gann, A, Levine, M and Losick, M 2013. Molecular biology of the gene. Benjamin-Cummings Publishing Company, New York, NY, USA.Google Scholar
Wijtten, PJA, Prak, R, Lemme, A and Langhout, DJ 2004. Effect of different dietary ideal protein concentrations on broiler performance. British Poultry Science 45, 504–511.CrossRefGoogle ScholarPubMed
Wu, G, Bazer, FW, Dai, Z, Li, D, Wang, J and Wu, Z 2014. Amino acid nutrition in animals: protein synthesis and beyond. Annual Review of Animal Biosciences 2, 387–417.CrossRefGoogle ScholarPubMed