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Effect of dietary enrichment with either n-3 or n-6 fatty acids on systemic metabolite and hormone concentration and ovarian function in heifers

Published online by Cambridge University Press:  13 May 2008

S. Childs ,
C. O. Lynch ,
A. A. Hennessy ,
C. Stanton ,
D. C. Wathes ,
J. M. Sreenan ,
M. G. Diskin  and
D. A. Kenny
S. Childs*
Affiliation:
Animal Production Research Centre, Teagasc, Mellows Campus, Athenry, Co. Galway, Ireland School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
C. O. Lynch
Affiliation:
Animal Production Research Centre, Teagasc, Mellows Campus, Athenry, Co. Galway, Ireland School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
A. A. Hennessy
Affiliation:
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
C. Stanton
Affiliation:
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
D. C. Wathes
Affiliation:
Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK
J. M. Sreenan
Affiliation:
Animal Production Research Centre, Teagasc, Mellows Campus, Athenry, Co. Galway, Ireland
M. G. Diskin
Affiliation:
Animal Production Research Centre, Teagasc, Mellows Campus, Athenry, Co. Galway, Ireland
D. A. Kenny
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
*
E-mail: [email protected]
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Abstract

The objective of this experiment was to examine the effects of dietary n-3 or n-6 fatty acid (FA) supplementation on blood FA, metabolite and hormone concentrations, follicle size and dynamics and corpus luteum (CL) size. Reproductively normal heifers (n = 24) were individually fed diets of chopped straw and concentrate containing either (i) no added lipid (CON; n = 8); (ii) 2% added fat as whole raw soya beans (WSB, n-6; n = 8); or (iii) 2% added fat as fish oil (FO, n-3; n = 8). Following oestrous cycle synchronisation, blood samples were collected at appropriate times and intervals for the measurement of hormones, FAs and metabolites. On days 15 and 16 of the cycle, animals were subjected to an intravenous oxytocin challenge and prostaglandin F2α (PGF2α) response, measured as venous concentrations of 13,14-dihydro-15-keto PGF2α (PGFM). Dry matter intake and average daily gain were similar among treatments (P > 0.05). Plasma concentration of linoleic acid was highest on WSB (P < 0.05), while eicosapentaenoic (EPA, n-3; P < 0.0001) and docosahexaenoic acid (DHA, n-3; P < 0.0001) were greatest in the FO group. Plasma concentrations of arachidonic acid were higher on FO (P < 0.05) compared with CON and WSB. Plasma triglyceride concentrations increased, while β-hydroxybutyrate (BHBA) decreased with time on all diets (P < 0.05). There was a diet × time interaction (P < 0.01) for non-esterified fatty acid (NEFA) concentrations. Plasma cholesterol was higher on WSB and FO (P < 0.01) compared with CON. Progesterone (P4) and oestradiol (E2) concentrations, as well as follicle growth rate and CL diameter were similar across diets (P > 0.05). There was a diet × day interaction for PGFM (P < 0.01). When corrected for systemic E2 : P4 ratio, day 15 concentrations of PGFM were higher in the WSB group at 15 and 30 min (P < 0.01) post oxytocin administration compared with CON and FO, which were similar (P > 0.05). Concentrations of PGFM on day 16 were similar for WSB and FO and were greater than CON at 15 (P < 0.01) and 45 min (P < 0.05) post oxytocin administration, and at 30 min for FO (P < 0.05). With the exception of PGFM, dietary lipid source did not affect the reproductive variables measured.

Keywords

fish oilcattleprostaglandin metaboliteprogesteronelinoleic acid
Type
Full Paper
Information
animal , Volume 2 , Issue 6 , June 2008 , pp. 883 - 893
Copyright
Copyright © The Animal Consortium 2008

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References

Abayasekara, DR, Wathes, DC 1999. Effects of altering dietary fatty acid composition on prostaglandin synthesis and fertility. Prostaglandins, Leukotrienes and Essential Fatty Acids 61, 275–287.CrossRefGoogle ScholarPubMed
Bauman, DE, Griinari, JM 2003. Nutritional regulation of milk fat synthesis. Annual Review of Nutrition 23, 203–227.CrossRefGoogle ScholarPubMed
Beam, SW, Butler, WR 1997. Energy balance and ovarian follicle development prior to the first ovulation postpartum in dairy cows receiving three levels of dietary fat. Biology of Reproduction 56, 133–142.CrossRefGoogle Scholar
Boland, MP, Lonergan, P, O’Callaghan, D 2001. Effect of nutrition on endocrine parameters, ovarian physiology, and oocyte and embryo development. Theriogenology 55, 1323–1340.CrossRefGoogle ScholarPubMed
Burke, JM, Carroll, DJ, Rowe, KE, Thatcher, WW, Stormshak, F 1996. Intravascular infusion of lipid into ewes stimulates production of progesterone and prostaglandin. Biology of Reproduction 55, 169–175.CrossRefGoogle ScholarPubMed
Burns, PD, Engle, TE, Harris, MA, Enns, RM, Whittier, JC 2003. Effect of fish meal supplementation on plasma and endometrial fatty acid composition in nonlactating beef cows. Journal of Animal Science 81, 2840–2846.CrossRefGoogle ScholarPubMed
Butler, WR 2001. Nutritional effects on resumption of ovarian cyclicity and conception rate in post partum dairy cows. British Society for Animal Science Occasional Publication 26, 133–145.CrossRefGoogle Scholar
Butler, WR, Cherney, DJR, Elrod, CC 1995. Milk urea nitrogen (MUN) analysis: Field trial results on conception rates and dietary inputs. In Cornell Nutrition Conference for Feed Manufacturers, p. 89. Cornell Univ, Ithaca, NY.Google Scholar
Cant, JP, Fredeen, AH, MacIntyre, T, Gunn, J, Crowe, N 1997. Effect of fish oil and monensin on milk composition in dairy cows. Canadian Journal of Animal Science 77, 125–131.CrossRefGoogle Scholar
Childs, S, Sreenan, JM, Hennessy, AA, Stanton, C, Diskin, MG, Kenny, DA 2007. Effect of dietary ω-3 polyunsaturated fatty acid supplementation on hormone and metabolite concentrations and corpus luteum size in beef heifers. Journal of Animal Science 85 (Suppl. 1), 526.Google Scholar
Dunne, LD, Diskin, MG, Boland, MP, O’ Farrell, KJ, Sreenan, JM 1999. The effect of pre- and post-insemination plane of nutrition on embryo survival in beef heifers. Animal Science 69, 411–417.CrossRefGoogle Scholar
Evans RD, Dillon P, Buckley F, Wallace M, Ducrocq V and Garrick D 2004. Trends in milk production, fertility and survival of Irish dairy cows as a result of the introgression of Holstein-Friesian genes. Proceedings of the Agricultural Research Forum, Tullamore, Ireland, Co. Offaly, Ireland.Google Scholar
Fahey, J, Mee, JF, Murphy, JJ, O’Callaghan, D 2002. Effects of calcium salts of fatty acids and calcium salt of methionine hydroxy analogue on plasma prostaglandin F2 alpha metabolite and milk fatty acid profiles in late lactation Holstein-Friesian cows. Theriogenology 58, 1471–1482.CrossRefGoogle ScholarPubMed
Filley, SJ, Turner, HA, Stormshak, F 1999. Prostaglandin f(2alpha) concentrations, fatty acid profiles, and fertility in lipid-infused postpartum beef heifers. Biology of Reproduction 61, 1317–1323.CrossRefGoogle ScholarPubMed
Filley, SJ, Turner, HA, Stormshak, F 2000. Plasma fatty acids, prostaglandin F2alpha metabolite, and reproductive response in postpartum heifers fed rumen bypass fat. Journal of Animal Science 78, 139–144.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M, Stanley, GHS 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Garcia-Bojalil, CM, Staples, CR, Risco, CA, Savio, JD, Thatcher, WW 1998. Protein degradability and calcium salts of long-chain fatty acids in the diets of lactating dairy cows: reproductive responses. Journal of Dairy Science 81, 1385–1395.CrossRefGoogle ScholarPubMed
Goff, AK 2004. Steroid hormone modulation of prostaglandin secretion in the ruminant endometrium during the estrous cycle. Biology of Reproduction 71, 11–16.CrossRefGoogle ScholarPubMed
Grummer, RR, Carroll, DJ 1991. Effects of dietary fat on metabolic disorders and reproductive performance of dairy cattle. Journal of Animal Science 69, 3838–3852.CrossRefGoogle ScholarPubMed
Guilbault, LA, Thatcher, WW, Collier, RJ, Wilcox, CJ, Drost, M 1985. Carry-over effects of periparturient endocrine changes on postpartum reproductive function of Holstein heifers bred to genetically different service sires. Journal of Animal Science 61, 1516–1526.CrossRefGoogle ScholarPubMed
Hawkins, DE, Niswender, KD, Oss, GM, Moeller, CL, Odde, KG, Sawyer, HR, Niswender, GD 1995. An increase in serum lipids increases luteal lipid content and alters the disappearance rate of progesterone in cows. Journal of Animal Science 73, 541–545.CrossRefGoogle ScholarPubMed
Hightshoe, RB, Cochran, RC, Corah, LR, Kiracofe, GH, Harmon, DL, Perry, RC 1991. Effects of calcium soaps of fatty acids on postpartum reproductive function in beef cows. Journal of Animal Science 69, 4097–4103.CrossRefGoogle ScholarPubMed
Homa, ST, Brown, CA 1992. Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes. Journal of Reproduction and Fertility 94, 153–160.CrossRefGoogle ScholarPubMed
Horan, B, Dillon, P, Faverdin, P, Delaby, L, Buckley, F, Rath, M 2005. The interaction of strain of Holstein-Friesian cows and pasture-based feed systems on milk yield, body weight, and body condition score. Journal of Dairy Science 88, 1231–1243.CrossRefGoogle ScholarPubMed
Jordan, E, Kenny, D, Hawkins, M, Malone, R, Lovett, DK, O’Mara, FP 2006. Effect of refined soy oil or whole soybeans on intake, methane output, and performance of young bulls. Journal of Animal Science 84, 2418–2425.CrossRefGoogle ScholarPubMed
Lammoglia, MA, Willard, ST, Oldham, JR, Randel, RD 1996. Effects of dietary fat and season on steroid hormonal profiles before parturition and on hormonal, cholesterol, triglycerides, follicular patterns, and postpartum reproduction in Brahman cows. Journal of Animal Science 74, 2253–2262.CrossRefGoogle ScholarPubMed
Lammoglia, MA, Willard, ST, Hallford, DM, Randel, RD 1997. Effects of dietary fat on follicular development and circulating concentrations of lipids, insulin, progesterone, estradiol-17 beta, 13,14-dihydro-15-keto-prostaglandin F(2 alpha), and growth hormone in estrous cyclic Brahman cows. Journal of Animal Science 75, 1591–1600.CrossRefGoogle Scholar
Lopes, AS, Butler, ST, Gilbert, RO, Butler, WR 2007. Relationship of pre-ovulatory follicle size, estradiol concentrations and season to pregnancy outcome in dairy cows. Animal Reproduction Science 99, 34–43.CrossRefGoogle ScholarPubMed
Lucy, MC, Gross, TS, Thatcher, WW 1990. Effects of intravenous infusion of a soybean oil emulsion on plasma concentration of 15-keto-13,14-dihydro-prostaglandin F2α and ovarian function in cycling Holstein heifers. In Livestock Reproduction in Latin America, pp. 119–132. International Atomic Energy Agency, Vienna, Austria.Google Scholar
Mattos, R, Staples, CR, Thatcher, WW 2000. Effects of dietary fatty acids on reproduction in ruminants. Reviews of Reproduction 5, 38–45.CrossRefGoogle ScholarPubMed
Mattos, R, Staples, CR, Williams, J, Amorocho, A, McGuire, MA 2002. Uterine, ovarian, and production responses of lactating dairy cows to increasing dietary concentrations of menhaden fish meal. Journal of Dairy Science 85, 755–764.CrossRefGoogle ScholarPubMed
Mattos, R, Guzeloglu, A, Badinga, L, Staples, CR, Thatcher, WW 2003. Polyunsaturated fatty acids and bovine interferon-tau modify phorbol ester-induced secretion of prostaglandin F2 alpha and expression of prostaglandin endoperoxide synthase-2 and phospholipase-A2 in bovine endometrial cells. Biology of Reproduction 69, 780–787.CrossRefGoogle ScholarPubMed
Mattos, R, Staples, CR, Arteche, A, Wiltbank, MC, Diaz, FJ, Jenkins, TC, Thatcher, WW 2004. The effects of feeding fish oil on uterine secretion of PGF2alpha, milk composition, and metabolic status of periparturient Holstein cows. Journal of Dairy Science 87, 921–932.CrossRefGoogle ScholarPubMed
Moussavi, AR, Gilbert, RO, Overton, TR, Bauman, DE, Butler, WR 2007. Effects of feeding fish meal and n-3 fatty acids on ovarian and uterine responses in early lactating dairy cows. Journal of Dairy Science 90, 145–154.CrossRefGoogle ScholarPubMed
Oldick, BS, Staples, CR, Thatcher, WW, Gyawu, P 1997. Abomasal infusion of glucose and fat – effect on digestion, production, and ovarian and uterine functions of cows. Journal of Dairy Science 80, 1315–1328.CrossRefGoogle ScholarPubMed
Park, PW, Goins, RE 1994. In situ preparation of fatty acid methyl esters for analysis of fatty acid composition in foods. Journal of Food Science 59, 1262–1266.CrossRefGoogle Scholar
Petit, HV, Dewhurst, RJ, Proulx, JG, Khalid, M, Haresign, W, Twagiramungu, H 2001. Milk production, milk composition, and reproductive function of dairy cows fed different fats. Canadian Journal of Animal Science 81, 263–271.CrossRefGoogle Scholar
Petit, HV, Dewhurst, RJ, Scollan, ND, Proulx, JG, Khalid, M, Haresign, W, Twagiramungu, H, Mann, GE 2002. Milk production and composition, ovarian function, and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science 85, 889–899.CrossRefGoogle ScholarPubMed
Petit, HV, Germiquet, C, Lebel, D 2004. Effect of feeding whole, unprocessed sunflower seeds and flaxseed on milk production, milk composition, and prostaglandin secretion in dairy cows. Journal of Dairy Science 87, 3889–3898.CrossRefGoogle ScholarPubMed
Prendiville, DJ, Enright, WJ, Crowe, MA, Finnerty, M, Hynes, N, Roche, JF 1995. Immunization of heifers against gonadotropin-releasing hormone: antibody titers, ovarian function, body growth, and carcass characteristics. Journal of Animal Science 73, 2382–2389.CrossRefGoogle ScholarPubMed
Rabiee, AR, Lean, IJ, Gooden, JM, Miller, BG, Scaramuzzi, RJ 1997. An evaluation of transovarian uptake of metabolites using arterio-venous difference methods in dairy cattle. Animal Reproduction Science 48, 9–25.CrossRefGoogle ScholarPubMed
Robinson, RS, Pushpakumara, PG, Cheng, Z, Peters, AR, Abayasekara, DR, Wathes, DC 2002. Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows. Reproduction 124, 119–131.CrossRefGoogle ScholarPubMed
Royal, M, Mann, GE, Flint, APF 2000. Strategies for reversing the trend towards subfertility in dairy cattle. The Veterinary Journal 160, 53–60.CrossRefGoogle ScholarPubMed
Sartori R, Souza AH, Piccinato CA, Sangsritavong S, Luchini D, Grummer RR and Wiltbank MC 2004. Effects of intra-abomasal infusion of linseed oil on steroid metabolism in dairy cows. In 15th International Congress on Animal Reproduction, Port Seguro, Brazil.Google Scholar
Scholljegerdes, EJ, Lake, SL, Weston, TR, Rule, DC, Moss, GE, Nett, TM, Hess, BW 2007. Fatty acid composition of plasma, medial basal hypothalamus, and uterine tissue in primiparous beef cows fed high-linoleate safflower seeds. Journal of Animal Science 85, 1555–1564.CrossRefGoogle ScholarPubMed
Staples, CR, Burke, JM, Thatcher, WW 1998. Influence of supplemental fats on reproductive tissues and performance of lactating cows. Journal of Dairy Science 81, 856–871.CrossRefGoogle ScholarPubMed
Sweeney, RA 1989. Generic combustion method for determination of crude protein in feeds: collaborative study. Association of Official Analytical Chemists 72, 770–774.Google ScholarPubMed
Van Soest, PJ, Robertson, JB, Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle ScholarPubMed
Wamsley, NE, Burns, PD, Engle, TE, Enns, RM 2005. Fish meal supplementation alters uterine prostaglandin F2{alpha} synthesis in beef heifers with low luteal-phase progesterone. Journal of Animal Science 83, 1832–1838.CrossRefGoogle ScholarPubMed
Wathes, DC, Abayasekara, DR, Aitken, RJ 2007. Polyunsaturated Fatty acids in male and female reproduction. Biology of Reproduction 77, 190–201.CrossRefGoogle ScholarPubMed
Wehrman, ME, Welsh, TH Jr, Williams, GL 1991. Diet-induced hyperlipidemia in cattle modifies the intrafollicular cholesterol environment, modulates ovarian follicular dynamics, and hastens the onset of postpartum luteal activity. Biology of Reproduction 45, 514–522.CrossRefGoogle ScholarPubMed
Whitlock, LA, Schingoethe, DJ, Hippen, AR, Kalscheur, KF, Baer, RJ, Ramaswamy, N, Kasperson, KM 2002. Fish oil and extruded soybeans fed in combination increase conjugated linoleic acids in milk of dairy cows more than when fed separately. Journal of Dairy Science 85, 234–243.CrossRefGoogle ScholarPubMed
Whitlock, LA, Schingoethe, DJ, AbuGhazaleh, AA, Hippen, AR, Kalscheur, KF 2006. Milk production and composition from cows fed small amounts of fish oil with extruded soybeans. Journal of Dairy Science 89, 3972–3980.CrossRefGoogle ScholarPubMed
Wistuba, TJ, Kegley, EB, Apple, JK 2006. Influence of fish oil in finishing diets on growth performance, carcass characteristics, and sensory evaluation of cattle. Journal of Animal Science 84, 902–909.CrossRefGoogle ScholarPubMed
Zeron, Y, Ocheretny, A, Kedar, O, Borochov, A, Sklan, D, Arav, A 2001. Seasonal changes in bovine fertility: relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. Reproduction 121, 447–454.CrossRefGoogle ScholarPubMed