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Effect of detoxified karanja (Pongamia spp.) cake on testicular architecture and semen production in ram lambs

Published online by Cambridge University Press:  19 July 2013

D. Dineshkumar ,
S. Selvaraju ,
S. Parthipan ,
Allen Thayakumar ,
D. Rajendran ,
J. P. Ravindra ,
P. Krishnamoorthy ,
I. J. Reddy  and
S. B. N. Rao
D. Dineshkumar
Affiliation:
Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
S. Selvaraju*
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
S. Parthipan
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
Allen Thayakumar
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
D. Rajendran
Affiliation:
Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
J. P. Ravindra
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
P. Krishnamoorthy
Affiliation:
Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore 560024, India
I. J. Reddy
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
S. B. N. Rao
Affiliation:
Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
*
†E-mail: [email protected]
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Abstract

The protein-rich non-conventional detoxified karanja cake (dKC) can be used in place of conventional protein supplements like soybean meal (SBM), groundnut meal, etc. in livestock feed. The present study was conducted to assess the effect of two levels of dKC by replacing SBM on testicular architecture, semen quality and expressions of mRNAs encoding luteinizing hormone receptor (LHR) and insulin-like growth factor (IGF-I) in testes of ram lambs. Eighteen ram lambs were randomly divided into three groups (n = 6) and fed different levels (%) of karanja cake (0% replacement – control; 50% replacement – dKC-50 and 75% replacement – dKC-75) for 140 days. After 120 days of feeding, the semen from the animals was collected and analysed. The testes samples were collected on day 140 of feeding for transcripts expression studies. The dKC-50 group had no change in BW, whereas dKC-75 group showed decreased (P < 0.05) BW as compared with control. The number of animals ejaculated semen in dKC-75 group was lower (P < 0.05) than the control group. A reduction (P < 0.05) in LHR expression in dKC-75 was observed, whereas a reduction in IGF-I expression (P < 0.05) was observed in dKC-50 and dKC-75 as compared with control group. The study reveals that in ram lambs, long-term feeding of dKC at 50% replacement of SBM may not affect BW. However, long-term feeding of dKC as a replacement of SBM may affect testicular function.

Keywords

karanja cakeram lambssemen qualityIGF-ILHR
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 10 , October 2013 , pp. 1697 - 1703
Copyright
Copyright © The Animal Consortium 2013 

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References

Akingbemi, BT, Sottas, CM, Koulova, AI, Klinefelter, GR, Hardy, MP 2004. Inhibition of testicular steroidogenesis by the xenoestrogen bisphenol A is associated with reduced pituitary luteinizing hormone secretion and decreased steroidogenic enzyme gene expression in rat Leydig cells. Endocrinology 145, 592–603.Google Scholar
Bancroft, JD, Steven, A 1996. Theory and practice of histological techniques. Churchill Livingstone, London.Google Scholar
Breier, BH, Gallaher, BW, Gluckman, PD 1991. Radioimmunoassay for insulin-like growth factor-I: solutions to some potential problems and pitfalls. Journal of Endocrinology 128, 347–357.Google Scholar
Bringi, NV, Mukerjee, SK 1987. Karanja seed (Pongamia glabra) oil. In Non-traditional oilseeds and oils in India (ed. NV Bringi), pp. 143–166. Oxford and IBH Publishing Co. Pvt. Ltd, New Delhi, India.Google Scholar
De Kretser, DM, Risbridger, GP, Kerr, JB 1995. Basic endocrinology of the testis. In Endocrinology (ed. LJ De Groot), pp. 2307–2335. WB Saunders Company, Philadelphia, Pennsylvania.Google Scholar
Dineshkumar, D, NageswaraRao, SB, Jash, S, Elangovan, AV, Hemalatha, S 2011. Chemical composition and anti nutritional factors in karanja (Pongamia pinnata) seed kernels and its in vitro evaluation. Indian Journal of Animal Science 81, 478–483.Google Scholar
Francis, GL, McNeil, KA, Wallace, JC, Ballard, FJ, Owens, PC 1989. Sheep insulin-like growth factors I and II: sequences, activities and assays. Endocrinology 124, 1173–1183.Google Scholar
Hansson, HA, Billig, H, Isgaard, J 1989. Insulin-like growth factor I in the developing and mature rat testis: immunohistochemical aspects. Biology of Reproduction 40, 1321–1328.Google Scholar
ICAR 1985. Nutrient requirements of livestock and poultry, pp. 5–7. ICAR, New Delhi, India.Google Scholar
Konwar, BK, Banerjee, GC, Mandal, L 1987. Effect of feeding deoiled karanj (Pongamia glabra vent) cake on quantity and quality of milk in crossbred cows. Indian Veterinary Journal 64, 62–65.Google Scholar
Lin, T, Haskell, J, Vinson, N, Terracio, L 1986. Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture. Biochemical and Biophysical Research Communications 137, 950–956.CrossRefGoogle ScholarPubMed
Livak, KJ, Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative pcr and the 2−ΔΔCT method. Methods 25, 402–408.Google Scholar
Mandal, 1985. Protease inhibitors and in vitro protein digestibility of defatted seed cakes of akashmoni and karanja. Journal of the American Oil Chemists Society 62, 1124–1126.Google Scholar
Murugesan, P, Muthusamy, T, Balasubramanian, K, Arunakaran, J 2007. Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells. Toxicology 232, 170–182.Google Scholar
Nagalakshmi, D, Dhanalakshmi, K, Himabindu, D 2011. Replacement of groundnut cake with sunflower and karanj seed cakes on performance, nutrient utilization, immune response and carcass characteristics in Nellore lambs. Small Ruminant Research 97, 12–20.Google Scholar
Panda, AK, Sastry, VRB, Mandal, AB, Saha, SK, Kumar, A 2004. Effect of dietary incorporation of raw and processed karanj cake (Pongamia glabra) on nutrient retention and immune competence of broiler chicks. Animal Nutrition and Feed Technology 4, 121–134.Google Scholar
Prabhu, TM, Deva Kumar, C, Sastry, VRB, Agarwal, DK 2002. Quantification of karanjin using high performance liquid chromatography in raw and detoxified karanj (Pongamia glabra vent) seed cake. Asian-Australasian Journal of Animal Sciences 15, 240–416.Google Scholar
Randel, RD, Chase, CC Jr, Wyse, SJ 1992. Effects of gossypol and cottonseed products on reproduction of mammals. Journal of Animal Science 70, 1628–1638.Google Scholar
Rangaswami, S, Seshadri, TR 1940. Pharmacy of Pongamia and Psorolea (solubility and their active principles in ordinary oils). Indian Journal of Pharmacology 2, 88–95.Google Scholar
Roy, D, Sharma, NN, Khanna, RN 1977. Structure and synthesis of isopongaflavone: a new component of seed of Pongamia glabra. Indian Journal of Chemistry 15, 1138–1139.Google Scholar
Selvaraju, S, Nandi, S, Sivasubramani, T, Raghavendra, BS, Rao, SB, Ravindra, JP 2010. Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: Effect of insulin-like growth factor-I. Theriogenology 73, 1–10.Google Scholar
Selvaraju, S, Sivasubramani, T, Raghavendra, BS, Raju, P, Rao, SBN, Dineshkumar, D, Ravindra, JP 2012a. Effect of dietary energy on seminal plasma insulin-like growth factor-I (IGF-I), serum IGF-I and testosterone levels, semen quality and fertility in adult rams. Theriogenology 78, 646–655.Google Scholar
Selvaraju, S, Raju, P, Rao, SBN, Raghavendra, BS, Nandi, S, Dineshkumar, D, Allen, thayakumar, Parthipan, S, Ravindra, JP 2012b. Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility. Reproduction Fertility and Development 24, 669–678.CrossRefGoogle ScholarPubMed
Singh, P, Sastry, VRB, Garg, AK, Sharma, AK, Singh, GR, Agrawal, DK 2006. Effect of long term feeding of expeller pressed and solvent extracted karanj (Pongamia pinnata) seed cake on the performance of lambs. Animal Feed Science and Technology 126, 157–167.CrossRefGoogle Scholar
Soren, NM, Sastry, VRB, Saha, SK, Wankhade, UD, Lade, MH, Kumar, A 2009. Performance of growing lambs fed processed karanj (Pongamia glabra) oil seed cake as partial protein supplement to soybean meal. Journal of Animal Physiology and Animal Nutrition 93, 237–244.Google Scholar
Srivastava, JP, Gupta, BS, Thakur, S, Verma, AK 1990. Utilization of deoiled karanj (Pongamia glabra) cake in kid grower rations. Indian Journal of Animal Nutrition 7, 15–20.Google Scholar
Vinay, BJ, Sindhu Kanya, TC 2008. Effect of detoxification on the functional and nutritional quality of proteins of karanja seed meal. Food Chemistry 106, 77–84.Google Scholar
Zung, A, Phillip, M, Chalew, SA, Palese, T, Kowarski, AA, Zadik, Z 1999. Testosterone effect on growth and growth mediators of the GH–IGF-I axis in the liver and epiphyseal growth plate of juvenile rats. Journal of Molecular Endocrinology 23, 209–221.Google Scholar
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