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Effect of Bacillus spp. direct-fed microbial on slurry characteristics and gaseous emissions in growing pigs fed with high fibre-based diets

Published online by Cambridge University Press:  14 July 2016

F. X. Prenafeta-Boldú ,
B. Fernández ,
M. Viñas ,
R. Lizardo ,
J. Brufau ,
A. Owusu-Asiedu ,
M. C. Walsh  and
A. Awati
F. X. Prenafeta-Boldú*
Affiliation:
GIRO Joint Research Unit IRTA-UPC, Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain
B. Fernández
Affiliation:
GIRO Joint Research Unit IRTA-UPC, Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain
M. Viñas
Affiliation:
GIRO Joint Research Unit IRTA-UPC, Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain
R. Lizardo
Affiliation:
IRTA–Mas de Bover, Ctra Reus-El Morell, Km 3,8. E-43120 Constantí, Tarragona, Spain
J. Brufau
Affiliation:
IRTA–Mas de Bover, Ctra Reus-El Morell, Km 3,8. E-43120 Constantí, Tarragona, Spain
A. Owusu-Asiedu
Affiliation:
Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough SN8 1XN, UK Anpario Plc, Manton Wood Enterprise Park Worksop, Nottinghamshire S80 2RS, UK
M. C. Walsh
Affiliation:
Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough SN8 1XN, UK
A. Awati
Affiliation:
Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough SN8 1XN, UK
*
†E-mail: [email protected]
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Abstract

A 26-day trial with 18 Pietrain×(Landrace×Duroc) pigs was conducted to investigate the effect of two dose levels of a specifically selected Bacillus spp. direct-fed microbial (DFM) product, on the emission of environmentally harmful gasses (methane, ammonia and hydrogen sulphide) from manure. Pigs were assigned to one of three treatments in a randomized complete block design according to their sex and initial BW. Each treatment contained three replications with two pigs per pen. The test treatments included a Bacillus spp. DFM containing 3×108 colony-forming unit/g, added at a low (250 mg/kg) and high (500 mg/kg) dose to an antibiotic free high fibre-based diet, and a non-supplemented control diet. Manure from pigs fed with the supplemented diets emitted lower amounts of atmospheric contaminants. The most significant reduction was observed with low DFM supplementation, in which methane and ammonia volatilization decreased (P<0.05) by >40% and 50%, respectively, on fresh weight basis in relation to the control. Microbiome analysis of manure by high through put sequencing techniques on eubacterial and archaeal 16S rRNA genes highlighted the complex interactions between indigenous gut microflora and inoculated Bacillus spp. The tested Bacillus DFM could be considered as a best available technique in reducing the environmental impacts of growing pigs fed with high fibre-based diets.

Keywords

anaerobic digestionBacillusdirect-fed microbialsgaseous emissionspig slurry
Type
Research Article
Information
animal , Volume 11 , Issue 2 , February 2017 , pp. 209 - 218
Copyright
© The Animal Consortium 2016 

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References

Angelidaki, I and Sanders, W 2004. Assessment of the anaerobic biodegradability of macropollutants. Reviews in Environmental Science and Biotechnology 3, 117–129.Google Scholar
APHA, AWA, and WEF 2005. Standard methods for the examination of water and waste water, 21st edition. American Public Health Association/American Water Works Association/Water Environment Federation, Washington, DC, USA.Google Scholar
Arnal, M-E, Zhang, J, Messori, S, Bosi, P, Smidt, H and Lallès, J-P 2014. Early changes in microbial colonization selectively modulate intestinal enzymes, but not inducible heat shock proteins in young adult swine. PLos One 9, e87967.CrossRefGoogle Scholar
Association of Analytical Communities (AOAC) 2003. Official methods of analysis of AOAC International, 17th edition, 2nd revision. Association of Analytical Communities, Gaithersburg, MD, USA.Google Scholar
Awati, A, Williams, BA, Bosch, MW, Gerrits, WJJ and Verstegen, MWA 2006. Effect of inclusion of fermentable carbohydrates in the diet on fermentation end-product profile in feces of weanling piglets. Journal of Animal Science 84, 2133–2140.Google Scholar
Barret, M, Gagnon, N, Topp, E, Masse, L, Massé, DI and Talbot, G 2013. Physico-chemical characteristics and methanogen communities in swine and dairy manure storage tanks: spatio-temporal variations and impact on methanogenic activity. Water Research 47, 737–746.Google Scholar
Boone, DR, Garrity, GM, Castenholz, RW, Brenner, DJ, Krieg, NR and Staley, JT 2009. Bergey’s manual of systematic bacteriology: the firmicutes. Springer, Dordrecht, the Netherlands.Google Scholar
Caporaso, JG, Kuczynski, J, Stombaugh, J, Bittinger, K, Bushman, FD, Costello, EK, Fierer, N, Gonzalez-Pena, A, Goodrich, JK, Gordon, JI, Huttley, GA, Kelley, ST, Knights, D, Koenig, JE, Ley, RE, Lozupone, CA, McDonald, D, Muegge, BD, Pirrung, M, Reeder, J, Sevinsky, JR, Turnbaugh, PJ, Walters, WA, Widmann, J, Yatsunenko, T, Zaneveld, J and Knight, R 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7, 335–336.CrossRefGoogle ScholarPubMed
Davis, ME, Parrott, T, Brown, DC, de Rodas, BZ, Johnson, ZB, Maxwell, CV and Rehberger, T 2008. Effect of a Bacillus-based direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs. Journal of Animal Science 86, 1459–1467.Google Scholar
de Angelis, M, Siragusa, S, Berloco, M, Caputo, L, Settanni, L, Alfonsi, G, Amerio, M, Grandi, A, Ragni, A and Gobbetti, M 2006. Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding. Research in Microbiology 157, 792–801.CrossRefGoogle ScholarPubMed
Demirel, B and Scherer, P 2008. The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review. Reviews in Environmental Science and Biotechnology 7, 173–190.CrossRefGoogle Scholar
Gerber, PJ, Steinfeld, H, Henderson, B, Mottet, A, Opio, C, Dijkman, J, Falcucci, A and Tempio, G 2013. Tackling climate change through livestock – a global assessment of emissions and mitigation opportunities. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.Google Scholar
Girault, R, Bridoux, G, Nauleau, F, Poullain, C, Buffet, J, Steyer, J, Sadowski, A and Béline, F 2012. A waste characterisation procedure for ADM1 implementation based on degradation kinetics. Water Research 46, 4099–4110.Google Scholar
Han, YK and Shin, HT 2005. Effects of antibiotics, copper sulfate and probiotics supplementation on the performance and ammonia emission from slurry in growing pigs. Journal of Animal Science and Technology 47, 537–546.Google Scholar
Hansen, K, Angelidaki, I and Ahring, B 1999. Improving thermophilic anaerobic digestion of swine manure. Water Research 33, 1805–1810.Google Scholar
International Panel on Climate Control (IPCC) 2003. Reference document on best available techniques for intensive rearing of poultry and pigs. European Comission, European IPPC Bureau-Seville. Retrieved on 17 February 2015 from http://eippcb.jrc.ec.europa.eu/reference/irpp.html.Google Scholar
International Panel on Climate Control (IPCC) 2006. Guidelines for National Greenhouse Gas Inventories, prepared by the National Greenhouse Gas Inventories programme (ed. HS Eggleston, L Buendia, K Miwa, T Ngara and K Tanabe), pp. 216–220. Institute for Global Environmental Strategies (IGES), Yamaguchi, Japan.Google Scholar
Lane, DJ 1991. 16S/23S sequencing. In Nucleic acid techniques in bacterial systematics (ed. E Stackebrandt and M Goodfellow), pp. 205–248. Wiley, Chichester, United Kingdom.Google Scholar
Liu, FH, Wang, SB, Zhang, JS, Zhang, J, Yan, X, Zhou, HK, Zhao, GP and Zhou, ZH 2009. The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. Journal of Applied Microbiology 106, 952–966.CrossRefGoogle Scholar
Loyon, L, Burton, CH, Misselbrook, T, Webb, J, Philippe, FX, Aguilar, M, Doreau, M, Hassouna, M, Veldkamp, T, Dourmad, JY, Bonmati, A, Grimm, E and Sommer, SG 2016. Best available technology for European livestock farms: availability, effectiveness and uptake. Journal of Environmental Management 166, 1–11.Google Scholar
Muyzer, G, Teske, A, Wirsen, CO and Jannasch, HW 1995. Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments. Archives of Microbiology 164, 165–172.CrossRefGoogle ScholarPubMed
Ndegwa, PM, Hristov, AN, Arogo, J and Sheffield, RE 2008. A review of ammonia emission mitigation techniques for concentrated animal feeding operations. Biosystems Engineering 100, 453–469.CrossRefGoogle Scholar
Nicholson, WL 2002. Roles of Bacillus endospores in the environment. Cellular and Molecular Life Sciences 59, 410–416.Google Scholar
Olivier, JGJ, Bouwman, AF, van der Hoek, KW and Berdowski, JJM 1998. Global air emission inventories for anthropogenic sources of NOx, NH3 and N2O in 1990. Environmental Pollution 102, 135–148.Google Scholar
Owusu-Asiedu, A, Jaworski, NW, Awati, AA and Stein, HH 2014. Effects of Bacillus spp. direct-fed microbial supplementation on the growth performance and nutrient digestibility in weaning pigs. ADSA/ASAS Midwest 2014 Meeting. Des Moines, IA, USA.Google Scholar
Pelletier, E, Kreimeyer, A, Bocs, S, Rouy, Z, Gyapay, G, Chouari, R, Rivière, D, Ganesan, A, Daegelen, P, Sghir, A, Cohen, GN, Médigue, C, Weissenbach, J and Le Paslier, D 2008. ‘Candidatus Cloacamonas acidaminovorans’: genome sequence reconstruction provides a first glimpse of a new bacterial division. Journal of Bacteriology 190, 2572–2579.CrossRefGoogle ScholarPubMed
Peu, P, Brugère, H, Pourcher, A-M, Kérourédan, M, Godon, J-J, Delgenès, J-P and Dabert, P 2006. Dynamics of a pig slurry microbial community during anaerobic storage and management. Applied and Environmental Microbiology 72, 3578–3585.CrossRefGoogle ScholarPubMed
Riviere, D, Desvignes, V, Pelletier, E, Chaussonnerie, S, Guermazi, S, Weissenbach, J, Li, T, Camacho, P and Sghir, A 2009. Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge. ISME Journal 3, 700–714.Google Scholar
Schreier, HJ 1993. Biosynthesis of glutamine and glutamate and the assimilation of ammonia. In Bacillus subtilis and other gram-positive bacteria (ed. AL Sonenshein, JA Hoch and R Losick), pp. 281–285. American Society of Microbiology, Washington, DC, USA.Google Scholar
Stackebrandt, E, Keddie, RM and Jones, D 2006. The genus Kurthia. Prokaryotes 4, 519–529.Google Scholar
Wang, Y, Cho, JH, Chen, YJ, Yoo, JS, Huang, Y, Kim, HJ and Kim, IH 2009. The effect of probiotic BioPlus 2B® on growth performance, dry matter and nitrogen digestibility and slurry noxious gas emission in growing pigs. Livestock Science 120, 35–42.Google Scholar
Wilms, R, Sass, H, Köpke, B, Cypionka, H and Engelen, B 2007. Methane and sulfate profiles within the subsurface of a tidal flat are reflected by the distribution of sulfate-reducing bacteria and methanogenic archaea. FEMS Microbiology Ecology 59, 611–621.Google Scholar
Wu, GD, Chen, J, Hoffmann, C, Bittinger, K, Chen, YY, Keilbaugh, SA, Bewtra, M, Knights, D, Walters, WA, Knight, R, Sinha, R, Gilroy, E, Gupta, K, Baldassano, R, Nessel, L, Li, H, Bushman, FD and Lewis, JD 2011. Linking long-term dietary patterns with gut microbial enterotypes. Science 334, 105–108.Google Scholar
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