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Do genetically modified crops affect animal reproduction? A review of the ongoing debate

Published online by Cambridge University Press:  28 January 2011

W. Zhang  and
F. Shi
W. Zhang
Affiliation:
Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, People's Republic of China
F. Shi*
Affiliation:
Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, People's Republic of China
*
†E-mail: [email protected]
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Abstract

In the past few years, genetically modified (GM) crops aimed at producing food/feed that became part of the regular agriculture in many areas of the world. However, we are uncertain whether GM food and feed can exert potential adverse effects on humans or animals. Of importance, the reproductive toxicology of GM crops has been studied using a number of methods, and by feeding GM crops to a number species of animals to ensure the safety assessment of GM food and feed. It appears that there are no adverse effects of GM crops on many species of animals in acute and short-term feeding studies, but serious debates of effects of long-term and multigenerational feeding studies remain. The aims of this review are to focus on the latest (last 3 to 4 years) findings and debates on reproduction of male and female animals after feeding daily diets containing the GM crops, and to present the possible mechanism(s) to explain their influences.

Keywords

genetically modified cropreproductive toxicityexogenous protein and DNAanti-nutrient compound
Type
Review
Information
animal , Volume 5 , Issue 7 , 31 May 2011 , pp. 1048 - 1059
Copyright
Copyright © The Animal Consortium 2011

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References

Albo, AG, Mila, S, Digilio, G, Motto, G, Aime, M, Corpillo, S 2007. Proteomic analysis of a genetically modified maize flour carrying Cry1Ab gene and comparison to the corresponding wild-type. Maydica 52, 443–455.Google Scholar
Alexander, TW, Reuter, T, Aulrich, K, Sharma, R, Okine, EK, Dixon, WT, Mcallister, T 2007. A review of the detection and fate of novel plant molecules derived from biotechnology in livestock production. Animal Feed Science and Technology 133, 31–62.CrossRefGoogle Scholar
Appenzeller, LM, Munley, SM, Hoban, D, Sykes, GP, Malley, LA, Delaney, B 2008. Subchronic feeding study of herbicide-tolerant soybean DP-356O43-5 in Sprague-Dawley rats. Food and Chemical Toxicology 46, 2201–2213.CrossRefGoogle ScholarPubMed
Appenzeller, LM, Munley, SM, Hoban, D, Sykes, GP, Malley, LA, Delaney, B 2009a. Subchronic feeding study of grain from herbicide-tolerant maize DP-O9814O-6 in Sprague-Dawley rats. Food and Chemical Toxicology 47, 2269–2280.CrossRefGoogle ScholarPubMed
Appenzeller, LM, Malley, L, Mackenzie, SA, Hoban, D, Delaney, B 2009b. Subchronic feeding study with genetically modified stacked trait lepidopteran and coleopteran resistant (DAS-O15O7-1xDAS-59122-7) maize grain in Sprague-Dawley rats. Food and Chemical Toxicology 47, 1512–1520.CrossRefGoogle ScholarPubMed
Ashworth, CJ, Toma, LM, Hunter, MG 2009. Nutritional effects on oocyte and embryo development in mammals: implications for reproductive efficiency and environmental sustainability. Philosophical Transactions of the Royal Society B: Biological Sciences 364, 3351–3361.CrossRefGoogle ScholarPubMed
Azeez, G, Nunan, C 2008. GM crops – the Health Effects, The Soil Association of UK. Bristol, UK. Retrieved April 10, 2010, from http://www.soilassociation.org/LinkClick.aspx?fileticket=SqDvBO1pyEU%3D&tabid=390.Google Scholar
Bakke-McKellep, AM, Koppang, EO, Gunnes, G, Sanden, M, Hemre, GI, Landsverk, T, Krogdahl, A 2007. Histological, digestive, metabolic, hormonal and some immune factor responses in Atlantic salmon, Salmo salar L., fed genetically modified soybeans. Journal of Fish Diseases 30, 65–79.CrossRefGoogle ScholarPubMed
Bakke-McKellep, AM, Sanden, M, Danieli, A, Acierno, R, Hemre, GI, Maffia, M, Krogdahl, A 2008. Atlantic salmon (Salmo salar L.) parr fed genetically modified soybeans and maize: Histological, digestive, metabolic, and immunological investigations. Research in Veterinary Science 84, 395–408.CrossRefGoogle ScholarPubMed
Batista, R, Oliveira, MM 2009. Facts and fiction of genetically engineered food. Trends in Biotechnology 27, 277–286.CrossRefGoogle ScholarPubMed
Bertrand, JA, Sudduth, TQ, Condon, A, Jenkins, TC, Calhoun, MC 2005. Nutrient content of whole cottonseed. Journal of Dairy Science 88, 1470–1477.CrossRefGoogle ScholarPubMed
Betz, FS, Hammond, BG, Fuchs, RL 2000. Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Regulatory Toxicology and Pharmacology 32, 156–173.CrossRefGoogle ScholarPubMed
Bondzio, A, Stumpff, F, Schon, J, Martens, H, Einspanier, R 2008. Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC) – a new in vitro model for safety assessment of recombinant food compounds. Food and Chemical Toxicology 46, 1976–1984.CrossRefGoogle Scholar
Brake, DG, Thaler, R, Evenson, DP 2004a. Evaluation of Bt (Bacillus thuringiensis) corn on mouse testicular development by dual parameter flow cytometry. Journal of Agricultural and Food Chemistry 52, 2097–2102.CrossRefGoogle ScholarPubMed
Brake, DG, Evenson, DP 2004b. A generational study of glyphosate-tolerant soybeans on mouse fetal, postnatal, pubertal and adult testicular development. Food and Chemical Toxicology 42, 29–36.CrossRefGoogle ScholarPubMed
Chowdhury, EH, Shimada, N, Murata, H, Mikami, O, Sultana, P, Miyazaki, S, Yoshioka, M, Yamanaka, N, Hirai, N, Nakajima, Y 2003a. Detection of Cry1Ab protein in gastrointestinal contents but not visceral organs of genetically modified Bt11-fed calves. Veterinary and Human Toxicology 45, 72–75.Google Scholar
Chowdhury, EH, Kuribara, H, Hino, A, Sultana, P, Mikami, O, Shimada, N, Guruge, KS, Saito, M, Nakajima, Y 2003b. Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11. Journal of Animal Science 81, 2546–2551.CrossRefGoogle ScholarPubMed
Chowdhury, EH, Mikami, O, Nakajima, Y, Hino, A, Kuribara, H, Suga, K, Hanazumi, M, Yomemochi, C 2003c. Detection of genetically modified maize DNA fragments in the intestinal contents of pigs fed StarLink CBH351. Veterinary and Human Toxicology 45, 95–96.Google ScholarPubMed
Cisterna, B, Flach, F, Vecchio, L, Barabino, SM, Battistelli, S, Martin, TE, Malatesta, M, Biggiogera, M 2008. Can a genetically-modified organism-containing diet influence embryo development? A preliminary study on pre-implantation mouse embryos. European Journal of Histochemistry 52, 263–267.CrossRefGoogle Scholar
Codex Alimentarius Commission (CAC) 2003a. Principles For The Risk Analysis of Foods Derived from Modern Biotechnology CAC/GL 44.Google Scholar
Codex Alimentarius Commission (CAC) 2003b. Guideline for the conduct of food safety assessment of foods derived recombinant-DNA plants CAC/GL 45.Google Scholar
Craig, W, Tepfer, M, Degrassi, G, Ripandelli, D 2008. An overview of general features of risk assessments of genetically modified crops. Euphytica 164, 853–880.CrossRefGoogle Scholar
Delaney, B, Zhang, J, Carlson, G, Schmidt, J, Stagg, B, Comstock, B, Babb, A, Finlay, C, Cressman, RF, Ladics, G, Cogburn, A, Siehl, D, Bardina, L, Sampson, H, Han, Y 2008a. A gene-shuffled glyphosate acetyltransferase protein from Bacillus licheniformis (GAT4601) shows no evidence of allergenicity or toxicity. Toxicological Sciences 102, 425–432.CrossRefGoogle ScholarPubMed
Delaney, B, Appenzeller, LM, Munley, SM, Hoban, D, Sykes, GP, Malley, LA, Sanders, C 2008b. Subchronic feeding study of high oleic acid soybeans (Event DP-3O5423-1) in Sprague-Dawley rats. Food and Chemical Toxicology 46, 3808–3817.CrossRefGoogle ScholarPubMed
Dhlamini, Z 2009. Agricultural Biotechnology. In Biosafety of genetically modified organisms: basic concepts, methods and issues (ed. MKA Chowdhury, MI Hoque and A Sonnino), pp. 1–50. FAO, Rome, Italy.Google Scholar
Dona, A, Arvanitoyannis, IS 2009. Health risks of genetically modified foods. Critical Reviews in Food Science and Nutrition 49, 164–175.CrossRefGoogle ScholarPubMed
European Food Safety Authority – Genetically Modified Organism (EFSA GMO) Panel 2008. Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. Food and Chemical Toxicology 46 (suppl.1), S2–S70.CrossRefGoogle Scholar
Ermakova, IV 2005. Influence of Genetically Modified Soya on the Birth Weight and Survival of Rat Pups: Preliminary Study. Retrieved May 10, 2010, from http://www.mindfully.org/GE/2005/Modified-Soya-Rats10oct05.htmGoogle Scholar
Ermakova, IV 2007. GM soybeans – revisiting a controversial format. Nature Biotechnology 25, 1351–1354.CrossRefGoogle ScholarPubMed
Evenson, DP, Darzynkiewicz, Z, Melamed, MR 1980. Comparison of human and mouse sperm chromatin structure by flow cytometry. Chromosoma 78, 225–238.CrossRefGoogle ScholarPubMed
Food and Agriculture Organization of the United Nations and World Health Organization (FAO/WHO) 2000. Safety aspects of genetically modified foods of plant origin. Report of a Joint FAO/WHO Expert Consultation on Foods Derived from Biotechnology, WHO, Geneva, Switzerland, 37pp.Google Scholar
Faust, MA, Glenn, BP 2002. Animal feeds from crops derived through biotechnology: farm animal performance and safety. In Biotechnology and Safety Assessment, 3rd edition (ed. JA Taylor and RL Fuchs), pp. 143–189. Academic Press, San Diego, CA, USA.Google Scholar
Finamore, A, Roselli, M, Britti, S, Monastra, G, Ambra, R, Turrini, A, Mengheri, E 2008. Intestinal and peripheral immune response to MON810 maize ingestion in weaning and old mice. Journal of Agricultural and Food Chemistry 56, 11533–11539.CrossRefGoogle ScholarPubMed
Francis, EZ, Kimmel, GL 1988. Proceeding of the workshop on One-vs -Two generation reproductive effects studies. International Journal of Toxicology 7, 911–925.Google Scholar
Guertler, P, Lutz, B, Kuehn, R, Meyer, HHD, Einspanier, R, Killerman, B, Albrecht, C 2008. Fate of recombinant DNA and Cry1Ab protein after ingestion and dispersal of genetically modified maizein comparison to rapeseed by fallow deer (Dama dama). European Journal of Wildlife Research 54, 36–43.CrossRefGoogle Scholar
Guimaraes, V, Drumare, MF, Lereclus, D, Gohar, M, Lamourette, P, Nevers, MC, Vaisanen-Tunkelrott, ML, Bernard, H, Guillon, B, Creminon, C, Wal, JM, Adel-Patient, K 2010. In vitro digestion of Cry1Ab proteins and analysis of the impact on their immunoreactivity. Journal of Agricultural and Food Chemistry 58, 3222–3231.CrossRefGoogle ScholarPubMed
Hammond, BG, Dudek, R, Lemen, JK, Nemeth, MA 2006. Results of a 90-day safety assurance study with rats fed grain from corn borer-protected corn. Food and Chemical Toxicology 44, 1092–1099.CrossRefGoogle ScholarPubMed
He, XY, Huang, KL, Li, X, Qin, W, Delaney, B, Luo, YB 2008. Comparison of grain from corn rootworm resistant transgenic DAS-59122-7 maize with non-transgenic maize grain in a 90-day feeding study in Sprague-Dawley rats. Food and Chemical Toxicology 46, 1994–2002.CrossRefGoogle Scholar
He, XY, Tang, MZ, Luo, YB, Li, X, Cao, SS, Yu, JJ, Delaney, B, Huang, KL 2009. A 90-day toxicology study of transgenic lysine-rich maize grain (Y642) in Sprague-Dawley rats. Food and Chemical Toxicology 47, 425–432.CrossRefGoogle ScholarPubMed
Healy, C, Hammond, B, Kirkpatrick, J 2008. Results of a 13-week safety assurance study with rats fed grain from corn rootworm-protected, glyphosate-tolerant MON 88017 corn. Food and Chemical Toxicology 46, 2517–2524.CrossRefGoogle ScholarPubMed
Heritage, J 2004. The fate of transgenes in the human gut. Nature Biotechnology 22, 170–172.CrossRefGoogle ScholarPubMed
Hirschi, KD 2009. Nutrient biofortification of food crops. Annual Review of Nutrition 29, 401–421.CrossRefGoogle ScholarPubMed
International Food Information Council (IFIC) 2008. Food Biotechnology: A Study of US consumer Attitudinal Trends Survey. IFIC. Retrieved April 10, 2010, from http://www.ificpubs.org/servlet/Detail?no=46Google Scholar
Jacobs, CM, Utterback, PL, Parsons, CM, Rice, D, Smith, B, Hinds, M, Liebergesell, M, Sauber, T 2008. Performance of laying hens fed diets containing DAS-59122-7 maize grain compared with diets containing nontransgenic maize grain. Poultry Science 87, 475–479.CrossRefGoogle ScholarPubMed
James, C 2007. Global Status of Commercialized Biotech/GM Crops: 2007. ISAAA Brief No. 37. International Service for the Acquisition of Agri-biotech Applications (ISAA). Ithaca, NY, USA, 16pp.Google Scholar
Juberg, DR, Herman, RA, Thomas, J, Brooks, KJ, Delaney, B 2009. Acute and repeated dose (28 day) mouse oral toxicology studies with Cry34Ab1 and Cry35Ab1 Bt proteins used in coleopteran resistant DAS-59122-7 corn. Regulatory Toxicology and Pharmacology 54, 154–163.CrossRefGoogle ScholarPubMed
Kaeppler, HF 2000. Food safety assessment of genetically modified crops. Agronomy Journal 92, 793–797.CrossRefGoogle Scholar
Keese, P 2008. Risks from GMOs due to horizontal gene transfer. Environmental Biosafety Research 7, 123–149.CrossRefGoogle ScholarPubMed
Kier, LD, Petrick, JS 2008. Safety assessment considerations for food and feed derived from plants with genetic modifications that modulate endogenous gene expression and pathways. Food and Chemical Toxicology 46, 2591–2605.CrossRefGoogle ScholarPubMed
Kilic, A, Akay, MT 2008. A three generation study with genetically modified Bt corn in rats: biochemical and histopathological investigation. Food and Chemical Toxicology 46, 1164–1170.CrossRefGoogle ScholarPubMed
Knudsen, I, Poulsen, M 2007. Comparative safety testing of genetically modified foods in a 90-day rat feeding study design allowing the distinction between primary and secondary effects of the new genetic event. Regulatory Toxicology and Pharmacology 49, 53–62.CrossRefGoogle Scholar
Koch, M, Strobel, E, Tebbe, CC, Heritage, J, Breves, G, Huber, K 2006. Transgenic maize in the presence of ampicillin modifies the metabolic profile and microbial population structure of bovine rumen fluid in vitro. British Journal of Nutrition 96, 820–829.CrossRefGoogle ScholarPubMed
Krishnan, H 2005. Engineering soybean for enhanced sulfur amino acid content. Crop Science 45, 454–461.CrossRefGoogle Scholar
Kwieciński, J 2009. Genetically modified abominations? Widespread opposition to GMOs might have deep-seated cultural causes. EMBO Reports 10, 1187–1190.CrossRefGoogle ScholarPubMed
Lutz, B, Wiedemann, S, Einspanier, R, Mayer, J, Albrecht, C 2005. Degradation of Cry1Ab protein from genetically modified maize in the bovine gastrointestinal tract. Journal of Agricultural and Food Chemistry 53, 1453–1456.CrossRefGoogle ScholarPubMed
MacKenzie, SA, Lamb, I, Schmidt, J, Deege, L, Morrisey, MJ, Harper, M, Layton, RJ, Prochaska, LM, Sanders, C, Locke, M, Mattsson, JL, Fuentes, A, Delaney, B 2007. Thirteen week feeding study with transgenic maize grain containing event DAS-O15O7-1 in Sprague-Dawley rats. Food and Chemical Toxicology 45, 551–562.CrossRefGoogle ScholarPubMed
Magana-Gomez, JA, delaBarca, AM 2009. Risk assessment of genetically modified crops for nutrition and health. Nutrition Reviews 67, 1–16.CrossRefGoogle ScholarPubMed
Malatesta, M, Biggiogera, M, Manuali, E, Rocchi, MB, Baldelli, B, Gazzanelli, G 2003. Fine structural analyses of pancreatic acinar cell nuclei from mice fed on genetically modified soybean. European Journal of Histochemistry 47, 385–388.CrossRefGoogle ScholarPubMed
Malatesta, M, Caporaloni, C, Gavaudan, S, Rocchi, MB, Serafini, S, Tiberi, C, Gazzanelli, G 2002a. Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean. Cell Structure and Function 27, 173–180.CrossRefGoogle ScholarPubMed
Malatesta, M, Caporaloni, C, Rossi, L, Battistelli, S, Rocchi, MB, Tonucci, F, Gazzanelli, G 2002b. Ultrastructural analysis of pancreatic acinar cells from mice fed on genetically modified soybean. Journal of Anatomy 201, 409–415.CrossRefGoogle ScholarPubMed
Malley, LA, Everds, NE, Reynolds, J, Mann, PC, Lamb, I, Rood, T, Schmidt, J, Layton, RJ, Prochaska, LM, Hinds, M, Locke, M, Chui, CF, Claussen, F, Mattsson, JL, Delaney, B 2007. Subchronic feeding study of DAS-59122-7 maize grain in Sprague-Dawley rats. Food and Chemical Toxicology 45, 1277–1292.CrossRefGoogle ScholarPubMed
Marshall, A 2007. GM soybeans and health safety – a controversy reexamined. Nature Biotechnology 25, 981–987.CrossRefGoogle ScholarPubMed
Mazza, R, Soave, M, Morlacchini, M, Piva, G, Marocco, A 2005. Assessing the transfer of genetically modified DNA from feed to animal tissues. Transgenic Research 14, 775–784.CrossRefGoogle ScholarPubMed
Mellon, M 2010. Food security: rigorous regulation required. Science 328, 171–172.CrossRefGoogle ScholarPubMed
Momma, K, Hashimoto, W, Yoon, HJ, Ozawa, S, Fukuda, Y, Kawai, S, Takaiwa, F, Utsumi, S, Murata, K 2000. Safety assessment of rice genetically modified with soybean glycinin by feeding studies on rats. Bioscience, Biotechnology, and Biochemistry 64, 1881–1886.CrossRefGoogle ScholarPubMed
Monsanto Company 2008. Monsanto Company Response to Austrian Report on Mouse Chronic and Reproduction Studies with NK603 × MON810 Maize. Retrieved April 28, 2010, from http://www.monsanto.com.ar/nuestros_productos/informacion_tecnica_seguridad/documentos/response_to_austrian_gm_study_20nov08.pdfGoogle Scholar
Netherwood, T, Martin-Orue, SM, O'Donnell, AG, Gockling, S, Graham, J, Mathers, JC, Gilbert, HJ 2004. Assessing the survival of transgenic plant DNA in the human gastrointestinal tract. Nature Biotechnology 22, 204–209.CrossRefGoogle ScholarPubMed
Organization for Economic Co-operation and development (OECD) 2007. Consensus document on safety information on transgenic plants expressing Bacillus Thuringiensis – derived insect control proteins. Series on Harmonization of Regulatory Oversight in Biotechnology, No. 42. OECD, Paris, France.Google Scholar
Onose, J, Imai, T, Hasumura, M, Ueda, M, Ozeki, Y, Hirose, M 2008. Evaluation of subchronic toxicity of dietary administered Cry1Ab protein from Bacillus thuringiensis var. Kurustaki HD-1 in F344 male rats with chemically induced gastrointestinal impairment. Food and Chemical Toxicology 46, 2184–2189.CrossRefGoogle ScholarPubMed
Park, S, Kang, TS, Kim, CK, Han, JS, Kim, S, Smith, RH 2005. Genetic manipulation for enhancing calcium content in potato tuber. Journal of Agricultural and Food Chemistry 53, 5598–5603.CrossRefGoogle ScholarPubMed
Parrott, W, Chassy, B, Ligon, J, Meyer, L, Petrick, J, Zhou, J, Herman, R, Delaney, B, Levine, M 2010. Application of food and feed safety assessment principles to evaluate transgenic approaches to gene modulation in crops. Food and Chemical Toxicology 48, 1773–1790.CrossRefGoogle ScholarPubMed
Paul, V, Steinke, K, Meyer, HH 2008. Development and validation of a sensitive enzyme immunoassay for surveillance of Cry1Ab toxin in bovine blood plasma of cows fed Bt-maize (MON810). Analytica Chimica Acta 607, 106–113.CrossRefGoogle ScholarPubMed
Paul, V, Guertler, P, Wiedemann, S, Meyer, HH 2010. Degradation of Cry1Ab protein from genetically modified maize (MON810) in relation to total dietary feed proteins in dairy cow digestion. Transgenic Research 19, 683–689.CrossRefGoogle ScholarPubMed
Powell, M, Wheatley, AO, Omoruyi, F, Asemota, HN, Williams, NP, Tennant, PF 2010. Comparative effects of dietary administered transgenic and conventional papaya on selected intestinal parameters in rat models. Transgenic Research 19, 511–518.CrossRefGoogle ScholarPubMed
Rasmussen, MA, Sarra, AC, Wilhelms, K, Scanes, CG 2007. Effects of Bt (Bacillus thuringiensis) corn on reproductive performance in adult laying hens. International Journal of Poultry Science 6, 169–171.CrossRefGoogle Scholar
Reuter, T, Aulrich, K 2003. Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feed-ingested foreign DNA in pig bodies. European Food Research and Technology 216, 185–192.CrossRefGoogle Scholar
Rhee, GS, Cho, DH, Won, YH, Seok, JH, Kim, SS, Kwack, SJ, Lee, RD, Chae, SY, Kim, JW, Lee, BM, Park, KL, Choi, KS 2005. Multigeneration reproductive and developmental toxicity study of bar gene inserted into genetically modified potato on rats. Journal of Toxicology and Environmental Health. Part A 68, 2263–2276.CrossRefGoogle ScholarPubMed
Rickard, C 2010. Response to ‘Health risks of genetically modified foods’ from Dona and Arvanitoyannis (2009) in Critical Reviews in Food Science and Nutrition (49:164-175). Critical Reviews in Food Science and Nutrition 50, 86–91.Google Scholar
Royal Society 2002. Genetically modified plants for food use and human health-an update. Policy document 4/02. Royal Society, London, UK.Google Scholar
Sanvido, O, Romeis, J, Bigler, F 2007. Ecological impacts of genetically modified crops: ten years of field research and commercial cultivation. Advances in Biochemical Engineering/Biotechnology 107, 235–278.CrossRefGoogle ScholarPubMed
Schrijver, AD, Devos, Y, Van den Bulcke, M, Cadot, P, De Loose, M, Reheul, D, Sneyers, M 2007. Risk assessment of GM stacked events obtained from crosses between GM events. Trends in Food Science & Technology 18, 101–109.CrossRefGoogle Scholar
Schroder, M, Poulsen, M, Wilcks, A, Kroghsbo, S, Miller, A, Frenzel, T, Danier, J, Rychlik, M, Emami, K, Gatehouse, A, Shu, QY, Engel, KH, Altosaar, I, Knudsen, I 2007. A 90-day safety study of genetically modified rice expressing Cry1Ab protein (Bacillus thuringiensis toxin) in Wistar rats. Food and Chemical Toxicology 45, 339–349.CrossRefGoogle ScholarPubMed
Séralini, GE, de Vendomois, JS, Cellier, D, Sultan, C, Buiatti, M, Gallagher, L, Antoniou, M, Dronamraju, KR 2009. How subchronic and chronic health effects can be neglected for GMOs, pesticides or chemicals. International Journal of Biological Sciences 5, 438–443.CrossRefGoogle ScholarPubMed
Sesikeran, B, Vasanthi, S 2008. Constantly evolving safety assessment protocols for GM foods. Asia Pacific Journal of Clinical Nutrition 17, 241–244.Google ScholarPubMed
Sharma, R, Damgaard, D, Alexander, TW, Dugan, ME, Aalhus, JL, Stanford, K, McAllister, TA 2006. Detection of transgenic and endogenous plant DNA in digesta and tissues of sheep and pigs fed Roundup Ready canola meal. Journal of Agricultural and Food Chemistry 54, 1699–1709.CrossRefGoogle ScholarPubMed
Shepherd, LV, McNicol, JW, Razzo, R, Taylor, MA, Davies, HV 2006. Assessing the potential for unintended effects in genetically modified potatoes perturbed in metabolic and developmental processes. Targeted analysis of key nutrients and anti-nutrients. Transgenic Research 15, 409–425.CrossRefGoogle ScholarPubMed
Singh, AK, Praveen, S, Singh, BP, Varma, A, Arora, N 2009. Safety assessment of leaf curl virus resistant tomato developed using viral derived sequences. Transgenic Research 18, 877–887.CrossRefGoogle ScholarPubMed
Stone, R 2008. China plans $3.5 billion GM crops initiative. Science 321, 1279.CrossRefGoogle ScholarPubMed
Trabalza-Marinucci, M, Brandi, G, Rondini, C, Avellini, L, Giammarini, C, Costarelli, S, Acuti, G, Orlandi, C, Filippini, G, Chiaradia, E, Malatesta, M, Crotti, S, Antoninia, C, Amagliani, G, Manuali, E, Mastrogiacomo, AR, Moscati, L, Naceur Haouet, M, Gaiti, A, Magnani, M 2008. A three-year longitudinal study on the effects of a diet containing genetically modified Bt176 maize on the health status and performance of sheep. Livestock Science 113, 178–191.CrossRefGoogle Scholar
Trosko, JE 2008. Role of diet and nutrition on the alteration of the quality and quantity of stem cells in human aging and the diseases of aging. Current Pharmaceutical Design 14, 2707–2718.CrossRefGoogle ScholarPubMed
van den Eede, G, Aarts, H, Buhk, HJ, Corthier, G, Flint, HJ, Hammes, W, Jacobsen, B, Midtvedt, T, van der Vossen, J, von Wright, A, Wackernagel, W, Wilcks, A 2004. The relevance of gene transfer to the safety of food and feed derived from genetically modified (GM) plants. Food and Chemical Toxicology 42, 1127–1156.CrossRefGoogle Scholar
Vecchio, L, Cisterna, B, Malatesta, M, Martin, TE, Biggiogera, M 2004. Ultrastructural analysis of testes from mice fed on genetically modified soybean. European Journal of Histochemistry 48, 448–454.Google ScholarPubMed
Velimirov, A, Binter, C, Zentek, J 2008. Biological Effects of Transgenic Maize NK603 × MON810 Fed in Long Term Reproduction Studies in Mice. Retrieved April 28, 2010, from http://www.biosicherheit.de/pdf/aktuell/zentek_studie_2008.pdfGoogle Scholar
WHO 2002. Foods Derived from Modern Technology: 20 Questions on Genetically Modified Foods. Retrieved October 10, 2010, from http://www.who.int/fsf/GMfood/Google Scholar
Wiedemann, S, Gurtler, P, Albrecht, C 2007. Effect of feeding cows genetically modified maize on the bacterial community in the bovine rumen. Applied and Environmental Microbiology 73, 8012–8017.CrossRefGoogle ScholarPubMed
Zhu, BT, Conney, AH 1998. Functional role of estrogen metabolism in target cells: review and perspectives. Carcinogenesis 19, 1–27.CrossRefGoogle ScholarPubMed