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Birth of the first mithun (Bos frontalis) calf through artificial insemination

Published online by Cambridge University Press:  13 May 2008

A. Dhali ,
M. Karunakaran ,
A. Mech ,
N. Nath ,
B. Prakash ,
C. Rajkhowa  and
D. P. Mishra
A. Dhali*
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
M. Karunakaran
Affiliation:
ICAR-RC-NEH Region, Medziphema, Nagaland 797 106, India
A. Mech
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
N. Nath
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
B. Prakash
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
C. Rajkhowa
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
D. P. Mishra
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
*
E-mail: [email protected]
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Abstract

The study describes the standardization of a suitable semen cryopreservation protocol for the first time in mithun (Bos frontalis) and birth of the first mithun calf through artificial insemination. The semen samples were collected from adult bulls through the rectal massage method and cryopreserved in liquid nitrogen using tris–egg yolk–glycerol diluent. The diluted semen samples were packaged in 0.50 ml straws and kept at 5°C for 4 h for equilibration. Following the equilibration, the straws were frozen into liquid nitrogen vapour for 10 min and then plunged into liquid nitrogen for storage. It was observed that the progressive motility (%) decreased significantly (P < 0.01) in cryopreserved semen (43.3 ± 4.1) compared with fresh samples (76.6 ± 3.3). The percentages of live spermatozoa (P < 0.01) and spermatozoa with intact acrosome (P < 0.05) also decreased significantly in cryopreserved semen (54.0 ± 3.3 and 64.6 ± 5.3) compared with fresh samples (79.3 ± 2.6 and 85.3 ± 1.8). Simultaneously, the total morphological abnormality (%) was found to be significantly (P < 0.01) higher in cryopreserved samples (15.46 ± 2.68) than in fresh semen (3.85 ± 0.63). A total of three mithun cows were inseminated using the cryopreserved semen. All the cows conceived following insemination and gave birth to healthy calves. The study revealed that mithun semen can be cryopreserved efficiently using tris–egg yolk–glycerol diluent, which can be further used for artificial insemination.

Keywords

semencryopreservationtris–egg yolk–glycerolmithunAI
Type
Full Paper
Information
animal , Volume 2 , Issue 6 , June 2008 , pp. 879 - 882
Copyright
Copyright © The Animal Consortium 2008

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References

Bhattacharya, HK, Goswami, BK, Bujarbaruah, KM, Deka, BC, Baishya, N, Sarma, BC 2005. Characteristics of semen collected by massage method in mithun (Bos frontalis) bulls. Indian Journal of Animal Science 75, 1168–1169.Google Scholar
Dee Leeuw, FE, Dee Leeuw, AM, Dean Daas, JHG, Colenbrander, B, Verkeij, AJ 1993. Effect of various cryoprotective agents and membrane stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology 30, 32–44.CrossRefGoogle Scholar
Fennessy, PF, Mackintosh, CG, Shackell, GH 1990. Artificial insemination of farmed red deer (Cervus elaphus). Animal Production 51, 613–621.Google Scholar
Foote, RH, Bratton, RW 1950. The fertility of bovine semen in extenders containing sulfanilamide, penicillin, streptomycin, and polymyxin. Journal of Dairy Science 33, 544–547.CrossRefGoogle Scholar
Foote, RH, Gray, LC, Young, DC, Dunn, HO 1960. Fertility of bull semen stored up to four days at 5°C in 20% egg yolk extenders. Journal of Dairy Science 43, 1330–1334.CrossRefGoogle Scholar
Garland, P 1989. Artificial insemination of scimitar-horned oryx (Oryx dammah). Bulletin of Zoo Management 27, 29–30.Google Scholar
Hammer, CJ, Tyler, HD, Loskutoff, NM, Armstrong, DL, Funk, DJ, Lindsey, BR, Simmons, LG 2001. Compromised development of calves (Bos gaurus) derived from in vitro-generated embryos and transferred interspecifically into domestic cattle (Bos taurus). Theriogenology 55, 1447–1455.CrossRefGoogle ScholarPubMed
Holt, WV 2000. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology 53, 47–58.CrossRefGoogle ScholarPubMed
Hopkins, SM, Armstrong, DL, Hummel, S, Junior, S 1988. Successful cryopreservation of gaur (Bos gaurus) epididymal spermatozoa. Journal of Zoo Animal Medicine 19, 195–201.CrossRefGoogle Scholar
Januskauskas, A, Lukoseviciute, K, Nagy, S, Johannisson, A, Rodriguez-martinez, H 2005. Assessment of the efficacy of Sephadex G-15 filtration of bovine spermatozoa for cryopreservation. Theriogenology 63, 160–178.CrossRefGoogle ScholarPubMed
Johnston, LA, Parrish, JJ, Monson, R, Leibfried-rutledge, L, Susko-parrish, JL, Northey, DL, Rutledge, JJ, Simmons, LG 1994. Oocyte maturation, fertilization and embryo development in vitro and in vivo in the gaur (Bos gaurus). Journal of Reproduction and Fertility 100, 131–136.CrossRefGoogle ScholarPubMed
Karunakaran, M, Dhali, A, Mech, A, Khate, K, Rajkhowa, C, Mishra, DP 2006. Preservation of mithun (Bos frontalis) semen at refrigeration temperature. Animal Reproduction Science 101, 257–264.CrossRefGoogle ScholarPubMed
Kovacs, A, Foote, RH 1992. Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotechnic and histochemistry 67, 119–124.CrossRefGoogle ScholarPubMed
Mondal, SK, Pal, DT 1999. Mithun: historical perspective. Asian Agri-History 3, 245–260.Google Scholar
Nagy, S, Hallap, T, Johannisson, A, Rodriguez-martinez, H 2004. Changes in plasma membrane and acrosome integrity of frozen thawed bovine spermatozoa during a 4 h incubation as measured by multicolor flow cytometry. Animal Reproduction Science 80, 225–235.CrossRefGoogle ScholarPubMed
Salisbury, GW, Fuller, HK, Willett, EL 1941. Preservation of bovine spermatozoa in yolk-citrate diluent and field results from its use. Journal of Dairy Science 24, 905–910.CrossRefGoogle Scholar
Simoons, FJ 1984. Gayal or mithun. In Evolution of domesticated animals (ed. IL Manson), pp. 34–36. Longman, London.Google Scholar
Somfai, T, Bodo, S, Nagy, S, Papp, AB, Ivancsics, J, Baranyai, B, Gocza, E, Kovacs, A 2002. Effect of swim up and Percoll treatment on viability and acrosome integrity of frozen-thawed bull spermatozoa. Reproduction in Domestic Animal 37, 285–290.CrossRefGoogle ScholarPubMed
SPSS 1999. SPSS® user’s guide: release 10.0.1 ed. SPSS Inc., Chicago, IL, USA.Google Scholar
Sukwongs Y, Kamolnorranath S, Lohachit C, Apimeteetumrong M, Yiengvisavakul V and Wolfe B 1998. Successful cryopreservation of gaur (Bibos gaurus) and banteng (Bibos javanicus) spermatozoa and pregnancies after artificial insemination with frozen semen. Proceedings of 36th Veterinary Conference, Kasetsart University, Bangkok, 8pp.Google Scholar
Thun, A, Hurtado, M, Janett, F 2002. Comparison of biociphos-plus® and tris-egg yolk extender for cryopreservation of bull semen. Theriogenology 57, 1087–1094.CrossRefGoogle ScholarPubMed
Vyas, S, Mohan, G, Dhami, AJ, Sahni, KL 1992. Effect of filtration through sephadex and glasswool on the quality and freezability of semen of crossbred bulls. Indian Journal of Animal Science 61, 341–343.Google Scholar