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Bioavailability of threonine and tryptophan in peanut meal for starter pigs using slope-ratio assay

Published online by Cambridge University Press:  01 May 2009

O. Adeola
O. Adeola*
Affiliation:
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907-2054, USA
*
E-mail: [email protected]
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Abstract

The objective of the two studies was to determine the bioavailability of threonine (thr) and tryptophan (trp) in peanut meal, relative to l-thr and l-trp, for starter pigs using the slope-ratio bioassay. Basal diets (BDs) contained surfeit amounts of all amino acids for 10 to 20 kg pigs, except for thr (Experiment 1) or trp (Experiment 2). In the first study, four reference diets were formulated by supplementing the BD with 0, 0.4, 0.8 or 1.2 g of l-thr/kg at the expense of cornstarch; two test diets were formulated by replacing cornstarch in the BD with peanut meal at 32 or 64 g/kg of diet to supply 0.4 or 0.8 g thr/kg, respectively. Four reference diets consisting of the BD supplemented with 0, 0.15, 0.3 or 0.45 g of l-trp/kg at the expense of cornstarch and two test diets in which cornstarch in the BD was replaced with peanut meal at 30 or 60 g/kg of diet to supply 0.14 or 0.28 g trp/kg, respectively were used in the second study. Body weight gain responded in a linear way to supplemental l-thr or thr from peanut meal (P < 0.001). There was a linear response (P < 0.001) to thr supplementation from l-thr or peanut meal in gain-to-feed ratio. The addition of trp to the BD linearly increased (P < 0.05) body weight gain, feed intake and gain-to-feed ratio regardless of the trp source. Common-intercept, multiple linear regression in slope-ratio methodology using weight gain or gain-to-feed ratio as dependent variables and supplemental thr intake as independent variable gave relative bioavailability estimates of 71.9% or 75.7%, respectively. Corresponding values for trp were 92% and 75.7%. The fiducial limits for none of the relative bioavailability estimates included 100%. The data from these studies suggest that the bioavailabilities of thr and trp in peanut meal are less than those of l-thr and l-trp, and that the bioavailabilities of thr and trp in peanut meal are 72% to 76% and 76% to 92%, respectively.

Keywords

amino acidspeanut mealpigsthreoninetryptophan
Type
Full Paper
Information
animal , Volume 3 , Issue 5 , May 2009 , pp. 677 - 684
Copyright
Copyright © The Animal Consortium 2009

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References

Adeola, O 1995. Dietary lysine and threonine utilization by young pigs: efficiency for carcass growth. Canadian Journal of Animal Science 75, 445–452.CrossRefGoogle Scholar
Adeola, O 1996. Bioavailability of tryptophan in soybean meal for 10-kg pigs using slope-ratio assay. Journal of Animal Science 74, 2411–2419.CrossRefGoogle ScholarPubMed
Adeola, O, Ball, RO 1992. Hypothalamic neurotransmitter concentrations and meat quality in stressed pigs offered excess dietary tryptophan and tyrosine. Journal of Animal Science 70, 1888–1894.CrossRefGoogle ScholarPubMed
Adeola, O, Lawrence, BV, Cline, TR 1994. Availability of amino acids for 10- to 20-kilogram pigs: lysine and threonine in soybean meal. Journal of Animal Science 72, 2061–2067.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists 2000. Official methods of analysis, 17th edition. AOAC, Arlington, VA, USA.Google Scholar
Batterham, ES 1992. Availability and utilization of amino acids for growing pigs. Nutrition Research Reviews 5, 1–18.CrossRefGoogle ScholarPubMed
Cortamira, NO, Seve, B, Lebreton, Y, Ganier, P 1991. Effect of dietary tryptophan on muscle, liver, and whole-body protein synthesis in weaned piglets: relationship to plasma insulin. British Journal of Nutrition 66, 423–435.CrossRefGoogle ScholarPubMed
Finney, DJ 1978. Statistical Methods in Biological Assay, 3rd edition. Charles Griffin & Co., London.Google Scholar
Folk, ED, Long, JP 1988. Serotonin as a neurotransmitter: a review. Comparative Biochemistry and Physiology 91C, 251–257.Google Scholar
Fuller, MF, McWilliam, R, Wang, TC, Giles, LR 1989. The optimum dietary amino acid pattern for growing pigs. 2. Requirements for maintenance and for tissue protein accretion. British Journal of Nutrition 62, 255–267.CrossRefGoogle ScholarPubMed
Goda, K, Hamane, Y, Kishimoto, R, Ogishi, Y 1999. Radical scavenging properties of tryptophan metabolites. Estimation of their radical reactivity. Advances in Experimental Medicine and Biology 467, 397–402.CrossRefGoogle ScholarPubMed
Guzik, AC, Southern, LL, Bidner, TD, Kerr, BJ 2002. The tryptophan requirement of nursery pigs. Journal of Animal Science 80, 2646–2655.Google ScholarPubMed
Han, Y, Chung, TK, Baker, DH 1993. Tryptophan requirement of pigs in the weight category 10 to 20 kilograms. Journal of Animal Science 71, 139–143.CrossRefGoogle ScholarPubMed
Henry, Y, Seve, B, Colleaux, Y, Ganier, P, Saligaut, C, Jego, P 1992. Interactive effects of dietary levels of tryptophan and protein on voluntary feed intake and growth performance in pigs in relation to plasma free amino acids and hypothalamic serotonin. Journal of Animal Science 70, 1873–1887.CrossRefGoogle ScholarPubMed
Humayun, MA, Elango, R, Moehn, S, Ball, RO, Pencharz, PB 2007. Application of the indicator amino acid oxidation technique for the determination of metabolic availability of sulfur amino acids from casein versus soy protein isolate in adult men. Journal of Nutrition 137, 1874–1879.CrossRefGoogle ScholarPubMed
Kovar, JL, Lewis, AJ, Radke, TR, Miller, PS 1993. Bioavailability of threonine in soybean meal for young pigs. Journal of Animal Science 71, 2133–2139.CrossRefGoogle ScholarPubMed
Leathwood, PD, Fernstrom, JD 1990. Effect of an oral tryptophan/carbohydrate load on tryptophan, large neutral amino acid, and serotonin and 5-hydroxyacetic acid levels in monkey brain. Journal of Neural Transmission 79, 25–34.CrossRefGoogle Scholar
Lewis, AJ, Bayley, HS 1995. Amino acid bioavailability. In Bioavailability of nutrients for animals: amino acids, minerals, and vitamins (ed. CB Ammerman, DH Baker and AJ Lewis), pp. 35–65. Academic Press, New York.CrossRefGoogle Scholar
Libao-Mercado, AJ, Leeson, S, Langer, S, Marty, BJ, de Lange, CF 2006. Efficiency of utilizing ileal digestible lysine and threonine for whole body protein deposition in growing pigs is reduced when dietary casein is replaced by wheat shorts. Journal of Animal Science 84, 1362–1374.CrossRefGoogle ScholarPubMed
Littell, RC, Lewis, AJ, Henry, PR 1995. Statistical evaluation of bioavailability assays. In Bioavailability of nutrients for animals: amino acids, minerals, and vitamins (ed. CB Ammerman, DH Baker and AJ Lewis), pp. 5–33. Academic Press, New York.CrossRefGoogle Scholar
Melchior, D, Sève, B, Le Floc’h, N 2004. Chronic lung inflammation affects plasma amino acid concentrations in pigs. Journal of Animal Science 82, 1091–1099.CrossRefGoogle ScholarPubMed
Moehn, S, Bertolo, RFP, Pencharz, PB, Ball, RO 2005. Development of the indicator amino acid oxidation technique to determine the availability of amino acids from dietary protein in pigs. Journal of Nutrition 135, 2866–2870.CrossRefGoogle ScholarPubMed
Moehn, S, Martinazzo-Dallagnol, E, Bertolo, RFP, Pencharz, PB, Ball, RO 2007. Metabolic availability of lysine in feedstuffs determined using oral isotope delivery. Livestock Science 109, 24–26.CrossRefGoogle Scholar
National Research Council 1998. Nutrient requirements of swine, 10th edition. National Academy Press, Washington, DC, USA.Google Scholar
Orsmbee, HS, Fondacaro, JD 1985. Action of serotonin on the gastrointestinal tract. Proceedings of the Society for Experimental Biology and Medicine 178, 333–338.Google Scholar
Ponter, AA, Seve, B, Morgan, LM 1994. Intragastric tryptophan reduces glycemia after glucose, and possibly via glucose-mediated insulinotropic polypeptide, in early weaned piglets. Journal of Nutrition 124, 259–267.CrossRefGoogle ScholarPubMed
Sato, H, Kobayashi, T, Jones, RW, Easter, RA 1987. Tryptophan availability of some feedstuffs determined by pig growth assay. Journal of Animal Science 64, 191–200.CrossRefGoogle ScholarPubMed
Schaart, MW, Schierbeek, H, van der Schoor, SR, Stoll, B, Burrin, DG, Reeds, PJ, van Goudoever, JB 2005. Threonine utilization is high in the intestine of piglets. Journal of Nutrition 135, 765–770.CrossRefGoogle ScholarPubMed
Schaechter, JD, Wurtman, RJ 1990. Serotonin release varies with brain tryptophan levels. Brain Research 532, 203–210.CrossRefGoogle ScholarPubMed
Statistical Analysis System Institute 2003. Statistical analysis system proprietary software. Release 9.1. SAS Institute Inc., Cary, NC, USA.Google Scholar
Wang, X, Qiao, SY, Liu, M, Ma, YX 2006. Effects of graded levels of true ileal digestible threonine on performance, serum parameters and immune function of 10–25 kg pigs. Animal Feed Science and Technology 129, 264–278.CrossRefGoogle Scholar
Wang, X, Qiao, S, Yin, Y, Yue, L, Wang, Z, Wu, G 2007. A deficiency or excess of dietary threonine reduces protein synthesis in jejunum and skeletal muscle of young pigs. Journal of Nutrition 137, 1442–1446.CrossRefGoogle ScholarPubMed
Watanabe, S, Togashi, S, Takahashi, N, Fukui, T 2002. l-tryptophan as an antioxidant in human placenta extract. Journal of Nutritional Science and Vitaminology 48, 36–39.CrossRefGoogle ScholarPubMed
Yen, JT, Kerr, BJ, Easter, RA, Parkhurst, AM 2004. Difference in rates of net portal absorption between crystalline and protein-bound lysine and threonine in growing pigs fed once daily. Journal of Animal Science 82, 1079–1090.CrossRefGoogle ScholarPubMed
Zhu, CL, Rademacher, M, de Lange, CFM 2005. Increasing dietary pectin level reduces utilization of digestible threonine intake, but not lysine intake, for body protein deposition in growing pigs. Journal of Animal Science 83, 1044–1053.CrossRefGoogle Scholar