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Are sainfoin or protein supplements alternatives to control small strongyle infection in horses?

Published online by Cambridge University Press:  24 May 2017

C. Collas ,
G. Sallé ,
B. Dumont ,
J. Cabaret ,
J. Cortet ,
W. Martin-Rosset ,
L. Wimel  and
G. Fleurance
C. Collas*
Affiliation:
Direction des Connaissances et de l’Innovation, IFCE, 49400 Saumur, France UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
G. Sallé
Affiliation:
UMR1282 Infectiologie et Santé Publique, INRA et Université François Rabelais Tours, 37380 Nouzilly, France
B. Dumont
Affiliation:
UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
J. Cabaret
Affiliation:
UMR1282 Infectiologie et Santé Publique, INRA et Université François Rabelais Tours, 37380 Nouzilly, France
J. Cortet
Affiliation:
UMR1282 Infectiologie et Santé Publique, INRA et Université François Rabelais Tours, 37380 Nouzilly, France
W. Martin-Rosset
Affiliation:
UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
L. Wimel
Affiliation:
Direction des Connaissances et de l’Innovation, IFCE, 49400 Saumur, France
G. Fleurance
Affiliation:
Direction des Connaissances et de l’Innovation, IFCE, 49400 Saumur, France UMR1213 Herbivores, INRA et Vetagro Sup, 63122 Saint-Genès-Champanelle, France
*
†E-mail: [email protected]
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Abstract

The spread of anthelmintic resistance in equine strongyle nematodes has become a major problem, advocating for the development of alternative control for strongyles. Our study consisted of both in vivo and in vitro experiments. We investigate for the first time the efficacy of a short-term consumption of tannin-rich sainfoin (Onobrychis viciifolia) or extra proteins in naturally infected horses. We used 30 horses allocated into three groups of 10 individuals that received for 18 days either (i) a tannin-rich diet with 70% DM sainfoin pellets (Sd), (ii) a protein-rich diet with 52% DM Italian rye-grass pellets and 18% DM grinded linseed expeller (Pd), or (iii) a control diet with 45% DM barley and 25% DM cereal-based pellets (Cd). The three diets were isoenergetic, covering 94% of animal energy requirements on average, and the Sd and Pd diets were isoproteic and provided extra proteins (227% of protein requirements v. 93% for the Cd diet). Pd and Cd were compared to test for benefits of receiving extra proteins, while Sd and Pd were compared to account for the effect of sainfoin secondary metabolites. There were no between-diet differences in faecal egg counts (FEC) or in worm burden evaluated from worm counts in faeces of drenched horses at the end of the experiment. However, coprocultures from the faeces collected in each group at the beginning and at the end of the experiment suggested a lower rate of strongyle larval development in the Sd group at the end of the experiment (Sd=8.1%, Pd=30.5%, Cd=22.6%). In vitro tests using sainfoin solutions evidenced the influence of sainfoin on strongyle larval development: adding 29% of sainfoin pellets to faeces reduced the strongyle egg development into infective larvae by 82% (P<0.001) and using solutions with sainfoin concentrations higher than 7.5 mg/ml reduced egg hatching by 37% (P<0.05). The short-term use of tannin-rich plants in horse diet could thus constitute a promising strategy to reduce the risk of infection by strongyles at pasture.

Keywords

equidtanninnitrogennematodenutrition
Type
Research Article
Information
animal , Volume 12 , Issue 2 , February 2018 , pp. 359 - 365
Copyright
© The Animal Consortium 2017 

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Footnotes

a

Present address: UR Animal et Fonctionnalités des Produits Animaux (USC340), INRA-Université de Lorraine, 54505 Vandœuvre-lès-Nancy Cedex, France.

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