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Apparent absorption of methionine and 2-hydroxy-4-methylthiobutanoic acid from gastrointestinal tract of conventional and gnotobiotic pigs

Published online by Cambridge University Press:  01 October 2009

G. Malik ,
D. Hoehler ,
M. Rademacher ,
M. D. Drew  and
A. G. Van Kessel
G. Malik
Affiliation:
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK, Canada
D. Hoehler
Affiliation:
Evonik Degussa GmbH, Rodenbacher Chaussee 4, 63457 Hanau-Wolfgang, Germany
M. Rademacher
Affiliation:
Evonik Degussa GmbH, Rodenbacher Chaussee 4, 63457 Hanau-Wolfgang, Germany
M. D. Drew
Affiliation:
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK, Canada
A. G. Van Kessel*
Affiliation:
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK, Canada
*
†E-mail: [email protected]
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Abstract

The effect of commensal microbiota and feeding corn or wheat/barley-based diets on the apparent gastrointestinal absorption of dl-methionine (MET) and 2-hydroxy-4-methylthiobutanoic acid (MHA-FA) was studied in conventional (n = 32) and gnotobiotic pigs (n = 24). Conventional pigs (CON) were vaginally delivered and sow-reared until weaning at 14 days of age. Gnotobiotic pigs were derived by caesarian section and reared in HEPA (high efficiency particulate air)-filtered isolator units with ad libitum access to a milk-based formula. Corn or wheat/barley-based diets were fed to all pigs from 14 to 24 days of age. At 24 days of age, after an overnight fast, pigs were fed 20 g/kg BW of experimental diet supplemented with 107 Bq of either 3H-l-MET or 3H-l-MHA-FA per kg of feed and chromic oxide (0.5% wt/wt). Pigs were killed for sample collection 3 h after consuming the meal. Residual 3H-MET and 3H-MHA-FA were estimated in gastrointestinal contents as the ratio of 3H : chromic oxide in digesta samples to the ratio of 3H : chromic oxide in feed. In CON pigs, feeding a wheat/barley-based diet increased (P < 0.05) total aerobes, whereas supplementation with MHA-FA increased (P < 0.05) total aerobes and lactobacilli populations in proximal small intestine (SI). Among the gnotobiotic pigs, bacterial contamination occurred such that eight pigs (two isolators) were monoassociated with a Gram-negative bacteria closely related to Providencia spp. and 16 pigs (four isolators) were monoassociated with Gram positive Enterococcus faecium. Species of monoassociated bacterial contaminant and diet composition did not affect residual methionine or MHA-FA in digesta. In both CON and monoassociated (MA) pigs, methionine and MHA-FA were retained in stomach (92%) but disappeared rapidly from proximal SI. Residual methionine and MHA-FA in digesta was not different in MA pigs; however, in CON pigs, less (P < 0.01) apparent residual methionine was found in digesta recovered at 25% (from cranial to caudal) and 75% of SI length compared with MHA-FA. Apparent residual methionine was 16% and 8% compared with 34% and 15% for MHA-FA, at the 25% and 75% locations, respectively. In proximal SI tissue, significantly (P < 0.05) higher radioactivity (cpm/mg wet tissue) was associated with MET pigs (8.56 ± 0.47) as compared to MHA-FA ones (5.45 ± 0.50). This study suggests that microbial metabolism of MHA-FA increases retention in small intestinal digesta relative to methionine and contributes, in part, to the lower bioefficacy of MHA-FA compared to methionine.

Keywords

amino acid transportdl-methioninemethionine hydroxy analogmicrobiotapigs
Type
Full Paper
Information
animal , Volume 3 , Issue 10 , October 2009 , pp. 1378 - 1386
Copyright
Copyright © The Animal Consortium 2009

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References

Cobb, MM, Holloway, BA, Rivers, JM 1991. Ascorbic acid catabolism by gut microflora: studies in germ-free and conventional guinea pigs. Journal of Nutritional Biochemistry 2, 635–643.CrossRefGoogle Scholar
Drew, MD, Van Kessel, AG, Maenz, DD 2003. Absorption of methionine and 2-Hydroxy-4-methylthiobutoanic acid in conventional and germ-free chickens. Poultry Science 82, 1149–1153.CrossRefGoogle ScholarPubMed
Dumonceaux, TJ, Hill, JE, Briggs, SA, Amoako, KK, Hemmingsen, SM, Van Kessel, AG 2006. Enumeration of specific bacterial populations in complex intestinal communities using quantitative PCR based on the chaperonin-60 target. Journal of Microbiological Methods 64, 46–62.CrossRefGoogle ScholarPubMed
Esteve Garcia, E, Austic, RE 1993. Intestinal absorption and renal excretion of dietary methionine sources by the growing chicken. Journal of Nutritional Biochemistry 4, 576–587.CrossRefGoogle Scholar
Falk, PG, Hooper, LV, Midvedt, T, Gordon, JI 1998. Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiolgy. Microbiology and Molecular Biology Reviews 62, 1157–1170.CrossRefGoogle Scholar
Fenton, TW, Fenton, M 1979. An improved procedure for the determination of chromic oxide in feed and feces. Canadian Journal of Animal Science 59, 631–634.CrossRefGoogle Scholar
Gonthier, MP, Verny, MA, Besson, C, Remesy, C, Scalbert, A 2003. Chlorogenic acid bioavailability largely depends on its metabolism by the gut microflora in rats. The Journal of Nutrition 133, 1853–1859.CrossRefGoogle ScholarPubMed
Hegedus, M, Andrasofszky, E, Brydll, E, Veresegyhazy, T, Tamas, J 1993. Biological activity of methionine derivatives: I. Microbiological activity of methionine derivatives. Magyar Állatorvosok Lapja 48, 527–531.Google Scholar
Hill, JE, Hemmingsen, SM, Blair, GG, Dumonceaux, TJ, Klassen, J, Zijlstra, RT, Goh, SH, Van Kessel, AG 2005. Comparison of ileum microflora of pigs fed corn-, wheat-, or barley-based diets by chaperonin-60 sequencing and quantitative PCR. Applied and Environmental Microbiology 71, 867–875.CrossRefGoogle ScholarPubMed
Hill, JE, Penny, SL, Crowell, KG, Goh, SH, Hemmingsen, SM 2004. cpnDB: a chaperonin sequence database. Genome Research 14, 1669–1675.CrossRefGoogle ScholarPubMed
Hill, JE, Seipp, RP, Betts, M, Hawkins, L, Van Kessel, AG, Crosby, WL, Hemmingsen, SM 2002. Extensive profiling of a complex microbial community using high throughput sequencing. Applied and Environmental Microbiology 68, 3055–3066.CrossRefGoogle ScholarPubMed
Jansman, AJM, Kan, CA, Wiebenga, J 2003. Comparison of the biological efficacy of dl-methionine and hydroxy-4-methylbutanoic acid (HMB) in pigs and poultry. CVB documentation report no. 29, Centraal Veevoederbureau, Lelystad, the Netherlands.Google Scholar
Jensen, BB, Jorgensen, H 1994. Effect of dietary fibre on microbial activity and microbial gas production in various regions of the gastrointestinal tract of pigs. Applied and Environmental Microbiology 60, 1897–1904.CrossRefGoogle ScholarPubMed
Lingens, G, Molnar, S 1996. Studies on metabolism of broilers by using 14C-labelled dl-methionine and dl-methionine hydroxy analogue Ca-salt. Archives of Animal Nutrition 49, 113–124.Google ScholarPubMed
Liu, S-Q, Holland, R, Crow, VL 2003. The potential of dairy lactic acid bacteria to metabolise amino acids via non-transaminating reactions and endogenous transamination. International Journal of Food Microbiology 86, 257–269.CrossRefGoogle ScholarPubMed
Maenz, DD, Engele Schaan, CM 1996a. Methionine and 2-hydroxy-4-methylthiobutanoic acid are partially converted to nonabsorbed compounds during passage through the small intestine and heat exposure does not affect small intestinal absorption of methionine sources in broiler chickens. The Journal of Nutrition 126, 1438–1444.CrossRefGoogle ScholarPubMed
Maenz, DD, Engele Schaan, CM 1996b. Methionine and 2-hydroxy-4-methylthiobutanoic acid are transported by distinct Na+-dependent and H+-dependent systems in the brush border membrane of the chick intestinal epithelium. The Journal of Nutrition 126, 529–536.CrossRefGoogle ScholarPubMed
National Research Council 1998. Nutrient Requirements of Swine, 10th edition. National Academic Press, Washington, DC.Google Scholar
Pieper, R, Jha, R, Rossnagel, B, Van Kessel, AG, Souffrant, W, Leterme, P 2008. Effect of barley and oat cultivars with different carbohydrate composition on the intestinal bacterial community composition in weaned piglets. FEMS Microbial Ecology 66, 556–566.CrossRefGoogle ScholarPubMed
Richards, JD, Atwell, CA, Vazquez-Anon, M, Dibner, JJ 2005. Comparative in vitro and in vivo absorption of 2-hydroxy-4(methylthio) butanoic acid and methionine in the broiler chicken. Poultry Science 84, 1397–1405.CrossRefGoogle ScholarPubMed
Riottot, M, Sacquet, E, Vila, JP, Leprince, C 1980. Relationship between small intestine transit and bile acid metabolism in axenic and holoxenic rats fed different diets. Reproduction and Nutrition Development 20, 163–171.CrossRefGoogle Scholar
Salter, DN, Coates, ME, Hewitt, D 1974. The utilization of protein and excretion of uric acid in germ-free and conventional chicks. British Journal of Nutrition 31, 307–318.CrossRefGoogle ScholarPubMed
Savage, DC 1986. Gastrointestinal microflora in mammalian nutrition. Annual Review of Nutrition 6, 155–178.CrossRefGoogle ScholarPubMed
Wester, TJ, Vazquez-Anon, M, Dibner, J, Parker, DS, Calder, AG, Lobley, GE 2006. Hepatic metabolism of 2-hydroxy-4-methylthiobutyrate in growing lambs. Journal of Dairy Science 89, 1062–1071.CrossRefGoogle ScholarPubMed
Winitz, M, Bloch-Frankenthal, L, Izumiya, N, Birnabaum, SN, Barker, CG, Greenstein, JP 1956. Studies on diastereoiomeric α-amino acids and correseponding α-hydroxy acids. VII. Influence of β-configuration on enzymatic susceptibility. Journal of American Chemical Society 78, 2423–2430.CrossRefGoogle Scholar