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Activation of caspase-9 and its influencing factors in beef during conditioning

Published online by Cambridge University Press:  05 December 2013

J. Cao ,
G. Zhou ,
Y. Liu ,
G. Liao ,
Q. Zhang ,
K. Ye ,
D. Pan  and
C. Ou
J. Cao
Affiliation:
Department of Food Science and Engineering, Ningbo University, Ningbo 315211, P. R. China National Center of Meat Quality and Safety Control, MOST, Nanjing Agricultural University, Nanjing 210095, P. R. China
G. Zhou
Affiliation:
National Center of Meat Quality and Safety Control, MOST, Nanjing Agricultural University, Nanjing 210095, P. R. China
Y. Liu
Affiliation:
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P. R. China
G. Liao
Affiliation:
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, P. R. China
Q. Zhang
Affiliation:
National Center of Meat Quality and Safety Control, MOST, Nanjing Agricultural University, Nanjing 210095, P. R. China
K. Ye
Affiliation:
National Center of Meat Quality and Safety Control, MOST, Nanjing Agricultural University, Nanjing 210095, P. R. China
D. Pan
Affiliation:
Department of Food Science and Engineering, Ningbo University, Ningbo 315211, P. R. China
C. Ou*
Affiliation:
Department of Food Science and Engineering, Ningbo University, Ningbo 315211, P. R. China
*
E-mail: [email protected]
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Abstract

To study the activation of caspase-9 and its potential influence in conditioning, longissimus thoracis (LT), semitendinosus (STN) and psoas minor (PMi) muscles were used to analyze the ratio of pro-apoptotic bax to anti-apoptotic bcl-2 in fresh tissues and observe the changes in ATP, cytosolic cytochrome c and caspase-9 activity levels during storage at 4°C. Caspase-9 activity at 5 h is higher than the activity at 0 and 24 h in the muscles (P<0.001). The ATP content decreased between 0 and 3 h, between 8 and 14 h in the PMi and LT muscles (P<0.0001), whereas between 0 and 5 h, between 8 and 14 h in the STN muscle (P<0.0001). There is 60.2%, 55.3% and 43.1% available ATP in the STN, LT and PMi muscles at 5 h, respectively. The cytosolic cytochrome c level increased during 5 and 24 h storage in the LT and PMi muscles (P<0.0001), during 5 and 96 h in the STN muscle (P<0.0001). The cytosolic cytochrome c at 24 h (P<0.001) and ratio of bax to bcl-2 (P<0.05) was higher in the PMi than in other muscles. We concluded that the increase in cytosolic cytochrome c and available intracellular ATP should be responsible for the increase in caspase-9 activity; the activation of caspase-9 could be limited by the subsequent depletion of ATP; the postmortem release level of cytochrome c could be determined by the ratio of bax to bcl-2 in fresh tissues.

Keywords

conditioningcaspase-9bovine skeletal musclescytochrome c releasebcl-2 family proteins
Type
Full Paper
Information
animal , Volume 8 , Issue 3 , March 2014 , pp. 504 - 509
Copyright
© The Animal Consortium 2013 

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Footnotes

a

The first two authors contribute equally to this study/work.

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