Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-23T07:03:06.420Z Has data issue: false hasContentIssue false
  • English
  • Français

Temporal expression of TnI fast and slow isoforms in biceps femoris and masseter muscle during pig growth

Published online by Cambridge University Press:  26 April 2010

Z. Y. Xu ,
H. Yang ,
Y. Li ,
Y. Z. Xiong  and
B. Zuo
Z. Y. Xu
Affiliation:
Key Laboratory of Swine Genetic and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China
H. Yang
Affiliation:
Key Laboratory of Swine Genetic and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China
Y. Li
Affiliation:
Key Laboratory of Swine Genetic and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China
Y. Z. Xiong
Affiliation:
Key Laboratory of Swine Genetic and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China
B. Zuo*
Affiliation:
Key Laboratory of Swine Genetic and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China
*
†E-mail: [email protected]
Get access

Abstract

Biceps femoris (BF) and masseter muscle (MM) are the mixture of slow oxidative and fast-twitch fibres. Compared with MM, BF had the significantly higher expression of myosin heavy chain (MyHC) fast IIx and IIb isoforms (MyHCIIx and MyHCIIb), but lower expression of MyHC slow isoform (MyHCI) and fast IIa isoform (MyHCIIa). The objective of this study was to investigate the expression pattern of troponin I (TnI) slow-twitch isoform (TNNI1) and fast-twitch isoform (TNNI2) in BF and MM of Yorkshire and Meishan pigs which differed significantly in the growth rate. The expression of the TNNI1 and TNNI2 peaked at the postnatal 35 days in Yorkshire pigs and postnatal 60 days in Meishan pigs. The expression of TNNI1 and TNNI2 in Meishan pigs was significantly higher than that in Yorkshire pigs at the foetal 60 days, while the opposite occurred at postnatal 35 days. The expression ratio of TNNI1 relative to TNNI2 favoured TNNI2 expression in BF and MM regardless of Yorkshire and Meishan pigs. TNNI1 expression in MM was significantly higher than that in BF at 60, 120 and 180 days in Meishan pigs and at 120 and 180 days in Yorkshire pigs. On the contrary, no significant difference of TNNI2 expression in BF and MM was found except for Yorkshire pigs of 180 days. This study provided the foundation for future research on TnI isoforms as the model gene to study mechanisms of muscle fibre-specific gene regulation in pigs.

Keywords

troponin Imyosin heavy chain (MyHC)biceps femorismasseterpig
Type
Full Paper
Information
animal , Volume 4 , Issue 9 , September 2010 , pp. 1541 - 1546
Copyright
Copyright © The Animal Consortium 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Banerjee-Basu, S, Buonanno, A 1994. Isolation and structure of the rat gene encoding troponin I (slow). Gene 145, 241–244.CrossRefGoogle ScholarPubMed
Bianospino, E, Moura, ASAMT, Wechsler, FS, Fernandes, S, Dal-Pai-Silva, M 2008. Age-related changes in muscle fiber type frequencies and cross-sectional areas in straightbred and crossbred rabbits. Animal 2, 1627–1632.CrossRefGoogle ScholarPubMed
Bhavsar, PK, Brand, NJ, Yacoub, MH, Barton, PJR 1996. Isolation and characterization of the human cardiac troponin I gene (TNNI3). Genomics 35, 11–23.CrossRefGoogle ScholarPubMed
Corin, SJ, Juhasz, O, Zhu, L, Conley, P, Kedes, L, Wade, R 1994. Structure and expression of the human slow twitch skeletal muscle troponin I gene. The Journal of Biological Chemistry 269, 10651–10659.CrossRefGoogle ScholarPubMed
Choi, YM, Kim, BC 2009. Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality. Livestock Science 122, 105–118.CrossRefGoogle Scholar
Froytein, T, Slinde, E, Standal, N 1981. Porcine stress and meat quality – causes and possible solutions to the problems. Agricultural Food Research Society, Norway, 369.Google Scholar
Greaser, ML, Gergely, J 1971. Reconstitution of troponin activity from three protein components. The Journal of Biological Chemistry 246, 4226–4233.CrossRefGoogle ScholarPubMed
Guenet, JL, Simon-Chazottes, D, Gravel, M, Hastings, KE, Schiaffino, S 1996. Cardiac and skeletal muscle troponin I isoforms are encoded by a dispersed gene family on mouse chromosomes 1 and 7. Mammalian Genome 7, 13–15.CrossRefGoogle ScholarPubMed
Kim, NK, Lim, JH, Song, MJ, Kim, OH, Park, BY, Kim, MJ, Hwang, IH, Lee, CS 2008. Comparisons of longissimus muscle metabolic enzymes and muscle fiber types in Korean and western pig breeds. Meat Science 78, 455–460.CrossRefGoogle ScholarPubMed
Lefaucheur, L, Milan, D, Ecolan, P, Le Callennec, C 2004. Myosin heavy chain composition of different skeletal muscles in Large White and Meishan pigs. Journal of Animal Science 82, 1931–1941.CrossRefGoogle ScholarPubMed
Livak, KJ, Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25, 402–408.CrossRefGoogle ScholarPubMed
Markham, TCW, Latorre, RM, Lawlor, PG, Ashton, CJ, McNamara, LB, Natter, R, Rowlerson, A, Stickland, NC 2009. Developmental programming of skeletal muscle phenotype/metabolism. Animal 3, 1001–1012.CrossRefGoogle ScholarPubMed
Murphy, AM, Thompson, WR, Peng, LF, Jones, IIL 1997. Regulation of the rat cardiac troponin I gene by the transcription factor GATA-4. Biochemical Journal 322, 393–401.CrossRefGoogle ScholarPubMed
Mullen, AJ, Barton, PJR 2000. Structural characterization of the human fast skeletal muscle troponin I gene (TNNI2). Gene 242, 313–320.CrossRefGoogle ScholarPubMed
Polly, P, Haddadi, LM, Issa, LL, Subramaniam, N, Palmer, SJ, Tay, ES, Hardeman, EC 2003. hMusTRD1 alpha 1 represses MEF2 activation of the Troponin I slow enhancer. The Journal of Biological Chemistry 278, 36603–36610.CrossRefGoogle Scholar
Schiaffino, S, Gorza, L, Ausoni, S 1993. Troponin isoform switching in the developing heart and its functional consequences. Trends in Cardiovascular Medicine 3, 12–17.CrossRefGoogle ScholarPubMed
Strom, D, Clemensson, E, Holm, S 1994. Fiber types and diameters in the porcine masseter muscle. A histochemical study. Acta Odontologica Scandinavica 52, 55–64.CrossRefGoogle ScholarPubMed
Xu, ZY, Xiong, YZ, Lei, MG, Li, FE, Zuo, B 2009. Genetic polymorphisms and preliminary association analysis with production traits of the porcine SLC27A4 gene. Molecular Biology Reports 36, 1427–1432.CrossRefGoogle ScholarPubMed
Yang, H, Xu, ZY, Ma, ZX, Xiong, YZ, Deng, CY, Zuo, B 2010. Molecular cloning and comparative characterization of the porcine troponin I family. Animal Biotechnology 21, 64–76.CrossRefGoogle ScholarPubMed
Zhang, ZG, Li, BD, Chen, XH 1986. Pig breeds in China. Shanghai Scientific and Technical Publisher, Shanghai, China.Google Scholar