Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T18:05:44.297Z Has data issue: false hasContentIssue false
  • English
  • Français

Supplemental progesterone during early pregnancy exerts divergent responses on embryonic characteristics in sows and gilts

Published online by Cambridge University Press:  07 January 2020

B. B. D. Muro Open the ORCID record for B. B. D. Muro [Opens in a new window] ,
R. F. Carnevale ,
D. F. Leal ,
M. A. Torres ,
M. V. Mendonça ,
D. H. Nakasone ,
C. H. G. Martinez ,
G. M. Ravagnani ,
M. S. Monteiro  and
A. P. Poor
...Show all authors
B. B. D. Muro*
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
R. F. Carnevale
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
D. F. Leal
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
M. A. Torres
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
M. V. Mendonça
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
D. H. Nakasone
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
C. H. G. Martinez
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
G. M. Ravagnani
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
M. S. Monteiro
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
A. P. Poor
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
S. M. M. K. Martins
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
P. Viau
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
C. A. Oliveira
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
L. H. Pulz
Affiliation:
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga13635-900, Brazil
R. F. Strefezzi
Affiliation:
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga13635-900, Brazil
G. W. Almond
Affiliation:
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC27606, USA
A. F. C. de Andrade
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo05508-270, Brazil
*
†E-mail: [email protected]
Get access

Abstract

Progesterone (P4) plays a key role in pregnancy establishment and maintenance; during early pregnancy, P4 stimulates the production and release of uterine secretions necessary for conceptus growth prior to implantation; therefore, exogenous P4 supplementation may improve embryo development. This study evaluated the effects of supplementation during early pregnancy with long-acting injectable progesterone or altrenogest on embryonic characteristics of sows and gilts. Thus, a total of 32 sows and 16 gilts were used. On day 6 of pregnancy sows and gilts were allocated to one of the following groups: non-supplemented; supplemented with 20 mg of altrenogest, orally, from days 6 to 12 of pregnancy; supplemented with 2.15 mg/kg of long-acting injectable progesterone on day 6 of pregnancy. Animals were killed on day 28 of pregnancy, and ovulation rate, embryo survival, embryo weight, crown-to-rump length, uterine glandular epithelium and endometrial vascularization were assessed. Treatments had no effect on pregnancy rate, embryo survival or endometrial vascular density (P > 0.05). Non-supplemented gilts presented larger and heavier embryos compared to gilts from supplemented groups (P < 0.05). Sows in the altrenogest group presented larger and heavier embryos compared to non-supplemented sows and sows supplemented with long-acting injectable progesterone. In conclusion, supplementation of sows and gilts with progestagen from day 6 of pregnancy can be used as a means to improve embryo survival without deleterious effects.

Keywords

embryo developmentuterine histoarchitectureprogestagenaltrenogestswine
Type
Research Article
Information
animal , Volume 14 , Issue 6 , June 2020 , pp. 1234 - 1240
Copyright
© The Animal Consortium 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bailey, DW, Dunlap, KA, Frank, JW, Erikson, DW, White, BG, Bazer, FW, Burghardt, RC and Johnson, GA 2010. Effect of long-term progesterone on developmental aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy. Reproduction 140, 583–594.CrossRefGoogle Scholar
Bazer, FW and Johnson, GA 2014. Pig blastocyst-uterine interactions. Differentiation 87, 52–65.CrossRefGoogle ScholarPubMed
Bazer, FW, Song, G, Kim, J, Dunlap, KA, Satterfield, MC and Johnson, GA 2012. Uterine biology in pigs and sheep. Journal of Animal Science and Biotechnology 3, 3–23.CrossRefGoogle ScholarPubMed
Carter, F, Forde, N, Duffy, P, Wade, M, Fair, T and Crowe, MA 2008. Effect of increasing progesterone concentration from day 3 of pregnancy on subsequent embryo survival and development in beef heifers. Reproduction Fertility and Development 20, 368–375.CrossRefGoogle ScholarPubMed
Chen, X, Fu, J and Wang, A 2016. Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs. Journal of Animal Science and Biotechnology 7, 31–43.CrossRefGoogle ScholarPubMed
Clemente, M, De La Fuente, J, Fair, T, Al Naib, A, Gutierrez-Adan, A, Roche, JF, Rizos, D and Lonergan, P 2009. Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium? Reproduction 138, 507–517.CrossRefGoogle ScholarPubMed
Da Silva, CLA, Van Den Brand, H, Laurenssen, BFA, Broekhuijse, ML, Knol, EF, Kemp, B and Soede, NM 2016. Relationships between ovulation rate and embryonic and placental characteristics in multiparous sows at 35 days of pregnancy. Animal 10, 1192–1199.CrossRefGoogle ScholarPubMed
Dunlap, KA, Burghardt, RC, Erikson, DW, Reed, KM, White, FJ, Farmer, JL, Spencer, TE, Magness, RR, Bazer, FW and Bayless, KJ 2008. Progesterone and placentation increase uterine glandular and stromal secreted phosphoprotein 1 (osteopontin) that may function for histotrophic and hematotrophic support of ovine pregnancy. Biology of Reproduction 79, 983–990.CrossRefGoogle ScholarPubMed
Forde, N, Carter, F, Fair, T, Crowe, MA, Evans, AC, Spencer, TE, Bazer, FW, McBride, R, Boland, MP, O’Gaora, P, Lonergan, P and Roche, JF 2009. Progesterone-regulated changes in endometrial gene expression contribute to advanced conceptus. Biology of Reproduction 81, 784–794.CrossRefGoogle ScholarPubMed
Garrett, JE, Geisert, RD, Zavy, MT and Morgan, GL 1988. Evidence for maternal regulation of early conceptus growth and development in beef cattle. Journal of Reproduction and Fertility 84, 437–446.CrossRefGoogle ScholarPubMed
Geisert, RD and Schmitt, RAM 2002. Early embryonic survival in the pig: Can it be improved? Journal of Animal Science 80, 54–65.Google Scholar
Gray, CA, Bartol, FF, Tarleton, BJ, Wiley, AA, Johnson, GA, Bazer, FW and Spencer, TE 2001. Developmental biology of uterine glands. Biology of Reproduction 65, 1311–1323.CrossRefGoogle ScholarPubMed
Ji, Y, Wu, Z, Dai, Z, Wang, X, Li, J, Wang, B and Wu, G 2017. Fetal and neonatal programming of postnatal growth and feed efficiency in swine. Journal of Animal Science and Biotechnology 87, 8–42.Google Scholar
Kirkden, RD, Broom, DM and Andersen, IL 2013. Piglet mortality: the impact of induction of farrowing using prostaglandins and oxytocin. Animal Reproduction Science 138, 14–24.CrossRefGoogle ScholarPubMed
Kridli, RT, Khalaj, K, Bidarimath, M and Tayade, C 2016. Placentation, maternal-fetal interface, and conceptus loss in swine. Theriogenology 85, 135–144.CrossRefGoogle ScholarPubMed
Mao, J and Foxcroft, GR 1998. Progesterone therapy during early pregnancy and embryonal survival in primiparous weaned sows. Journal of Animal Science 76, 1922–1928.CrossRefGoogle ScholarPubMed
Mathew, DJ, Sellner, EM, Green, JC, Okamura, CS, Anderson, LL, Lucy, MC and Geisert, RD 2011. Uterine progesterone receptor expression, conceptus development, and ovarian function in pigs treated with RU 486 during early pregnancy. Biology of Reproduction 84, 130–139.CrossRefGoogle ScholarPubMed
Muns, R, Nuntapaitoon, M and Tummaruk, P 2016. Non-infectious causes of pre-weaning mortality in piglets. Livestock Science 184, 46–57.CrossRefGoogle Scholar
Novak, S, Treacy, BK, Almeida, FRCL, Mao, J, Buhi, WC, Dixon, WT and Foxcroft, GR 2002. Regulation of IGF-I and porcine oviductal secretory protein (pOSP) secretion into the pig oviduct in the peri-ovulatory period, and effects of previous nutrition. Reproduction, Nutrition and Development 42, 355–372.CrossRefGoogle ScholarPubMed
Okrasa, S, Franczak, A, Zmijewska, A, Wojciechowic, B, Dziekonski, M, Martyniak, M, Kolakowska, J, Zglejc, K and Kotwica, G 2014. The uterine secretory activity and its physiological changes in the pig. Acta Biologica Cracoviensia 55/56, 40–57.Google Scholar
Patterson, J, Wellen, A, Hahn, M, Pasternak, A, Lowe, J, DeHass, S, Kraus, D, Williams, N and Foxcroft, G 2008. Responses to delayed estrus after weaning in sows using oral progestagen treatment. Journal of Animal Science 86, 1996–2004.CrossRefGoogle ScholarPubMed
Satterfield, MC, Bazer, FW and Spencer, TE 2006. Progesterone regulation of preimplantation conceptus growth and galectin 15 (LGALS15) in the ovine uterus. Biology of Reproduction 75, 289–296.CrossRefGoogle ScholarPubMed
Soede, NM, Bouwman, EG, Van der Laan, I, Hazegeler, W, Jourquin, J, Langendijk, P and Kemp, B 2012. Progestagen supplementation during early pregnancy does not improve embryo survival in pigs. Reproduction in Domestic Animals 47, 835–841.CrossRefGoogle Scholar
Soede, NM, Langendijk, P and Kemp, B 2011. Reproductive cycle in pigs. Animal Reproduction science 124, 251–258.CrossRefGoogle ScholarPubMed
Spencer, TE, Forde, N and Lonergan, P 2015. The role of progesterone and conceptus-derived factors in uterine biology during early pregnancy in ruminants. Journal of Dairy Science 99, 5941–5950.CrossRefGoogle ScholarPubMed
Spencer, TE, Johnson, GA, Burghardt, RC and Bazer, FW 2004. Progesterone and placental hormone actions on the uterus: insights from domestic animals. Biology of Reproduction 71, 2–10.CrossRefGoogle ScholarPubMed
Starbuck, G, Darwash, A, Mann, G and Lamming, G 2001. The detection and treatment of post insemination progesterone insufficiency in dairy cows. BSAP Occasional Publication 26, 447–450.CrossRefGoogle Scholar
Sukjumlong, S, Dalin, A, Sahlin, L and Persson, E 2005. Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the estrous cycle and in inseminated sows at estrus and early pregnancy. Reproduction 129, 349–359.CrossRefGoogle Scholar
Szymanska, M and Blitek, A 2016. Endometrial and conceptus response to exogenous progesterone treatment in early pregnant gilts following hormonally-induced estrus. Animal Reproduction Science 174, 56–64.CrossRefGoogle ScholarPubMed
Vallet, JL, Christenson, RK, Trout, WE and Klemcke, HG 1998. Conceptus, progesterone, and breed effects on uterine protein secretion in swine. Journal of Animal Science 76, 2657–2670.CrossRefGoogle ScholarPubMed
Van Der Waaij, EH, Hazeleger, W, Soede, NM, Laurenssen, BFA and Kemp, B 2010. Effect of excessive, hormonally induced intrauterine crowding in the gilt on fetal development on day 40 of pregnancy. Journal of Animal Science 88, 2611–2619.CrossRefGoogle ScholarPubMed
Wang, J, Feng, C, Liu, T, Shi, M, Wu, G and Bazer, FW 2017. Physiological alterations associated with intrauterine growth restriction in fetal pigs: causes and insights for nutritional optimization. Molecular Reproduction and Development 84, 897–904.CrossRefGoogle ScholarPubMed
Supplementary material: File

Muro et al. supplementary material

Muro et al. supplementary material

Download Muro et al. supplementary material(File)
File 1.3 MB