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Phyto-oestrogens in herbage and milk from cows grazing white clover, red clover, lucerne or chicory-rich pastures

Published online by Cambridge University Press:  01 August 2009

C. Andersen ,
T. S. Nielsen ,
S. Purup ,
T. Kristensen ,
J. Eriksen ,
K. Søegaard ,
J. Sørensen  and
X. C. Fretté
C. Andersen
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
T. S. Nielsen
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
S. Purup*
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
T. Kristensen
Affiliation:
Department of Agroecology and Environment, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
J. Eriksen
Affiliation:
Department of Agroecology and Environment, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
K. Søegaard
Affiliation:
Department of Agroecology and Environment, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
J. Sørensen
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
X. C. Fretté
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, Tjele, Denmark
*
†E-mail: [email protected]
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Abstract

A grazing experiment was carried out to study the concentration of phyto-oestrogens in herbage for cattle and in milk during two periods (May and June). Forty-eight Danish Holstein cows were divided into four groups with four treatment diets; white clover, red clover, lucerne and chicory-rich pastures. Each experimental period lasted 15 days. Herbage samples from the first day and individual milk samples from the last day of the experimental period were analysed for phyto-oestrogens using LC-MS technique. The total concentration of phyto-oestrogens was 21 399 mg/kg dry matter (DM) for red clover and 238 to 466 mg/kg DM for the other three herbages mainly due to a much higher concentration of biochanin A, formononetin and glycitein in red clover. In the milk, the total concentration of phyto-oestrogens was 253 to 397 μg/l for red clover milk and 56 to 91 μg/l in the milk from the other three treatments. This was especially due to a higher concentration of equol, daidzein and formononetin in the red clover milk. The concentration of biochanin A was significantly higher in milk from the red clover treatment in May while no differences were observed in June. Enterodiol was similar across treatments while the concentration of enterolactone was significantly lower for red clover milk compared with the other treatments. Of the tested pastures, red clover appears to have the highest concentration and to be the best source of phyto-oestrogens, especially equol, in bovine milk.

Keywords

phyto-oestrogensbovine milkpasture
Type
Full Paper
Information
animal , Volume 3 , Issue 8 , August 2009 , pp. 1189 - 1195
Copyright
Copyright © The Animal Consortium 2009

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References

Andersen, C, Hansen-Møller, J, Weisbjerg, MR, Sejrsen, K 2009. The content of phyto-oestrogens in milk and feedstuffs from cows fed clover grass silage or different concentrations of alfalfa silage. Animal 3, 617–622.CrossRefGoogle Scholar
Antignac, JP, Cariou, R, Le Bizec, B, André, F 2004. New data regarding phytoestrogens content in bovine milk. Food Chemistry 87, 275–281.CrossRefGoogle Scholar
Benassayag, C, Perrot-Applanat, M, Ferre, F 2002. Phytoestrogens as modulators of steroid action in target cells. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 777, 233–248.CrossRefGoogle ScholarPubMed
Booth, NL, Cassia, RO, Ping, Y, Totura, S, Deng, Y, Hedayat, AS, Bolton, JL, Pauli, GF, Farnsworth, NR 2006. Seasonal variation of red clover (Trifolium pratense L., Fabaceae) isoflavones and estrogenic activity. Journal of Agricultural and Food Chemistry 54, 1277–1282.CrossRefGoogle ScholarPubMed
Committee on Toxicity of Chemicals in Food 2003. COT report – phytoestrogens and health. Food Standards Agency, London.Google Scholar
Eriksen, J, Søegaard, K, Kristensen, T, Fretté, XC, Wiking, L, Nielsen, JH 2007. Herbage and milk productivity and quality when grazing different legumes and herbs. Grassland Science in Europe 12, 212–214.Google Scholar
Hoikkala, A, Mustonen, E, Saastmoinen, I, Jokela, T, Teponen, J, Saloniemi, H, Wähälä, K 2007. High levels of equol in organic skimmed Finnish cow milk. Molecular Nutrition & Food Research 51, 782–786.CrossRefGoogle ScholarPubMed
Kim, HA, Jeong, KS, Kim, YK 2008. Soy extract is more potent than genistein on tumor growth inhibition. Anticancer Research 28, 2837–2841.Google ScholarPubMed
Kurzer, MS, Xu, X 1997. Dietary phytoestrogens. Annual Review of Nutrition 17, 353–381.CrossRefGoogle ScholarPubMed
Lundh, T 1995. Metabolism of estrogenic Isoflavones in domestic animals. Proceedings of the Society of Experimental Biology and Medicine 208, 33–39.CrossRefGoogle ScholarPubMed
Purup, S, Hansen-Møller, J, Sejrsen, K, Christensen, LP, Lykkesfeldt, AE, Leffers, H, Skakkebæk, NE 2005. Increased phytoestrogen content in organic milk and the biological importance. Newsletter from Danish Research Centre for Organic Farming, No. 2. Danish Research Centre for Organic Farming, Tjele, Denmark.Google Scholar
Saloniemi, H, Wähälä, K, Nykänen-Kurki, P, Kallela, K, Saastamoinen, I 1995. Phytoestrogen content and estrogenic effect of legume fodder. Proceedings of the Society for Experimental Biology and Medicine 208, 13–17.CrossRefGoogle ScholarPubMed
SAS Institute 1999. SAS/STAT Software. Release 8.02. SAS Institute Inc., Cary, NC, USA.Google Scholar
Simonsen, F 2006. Analyseteknik, instrumentering og metoder, 2nd edition. Nyt Teknisk Forlag, Copenhagen V, Denmark.Google Scholar
Sivesind, E, Seguin, P 2005. Effects of the environment, cultivar, maturity, and preservation method on red clover isoflavone concentration. Journal of Agriculture and Food Chemistry 53, 6397–6402.CrossRefGoogle ScholarPubMed
Steinshamn, H, Purup, S, Thuen, E, Hansen-Møller, J 2008. Effects of clover silages and concentrate supplementation on the content of phytoestrogens in dairy cow milk. Journal of Dairy Science 91, 2715–2725.CrossRefGoogle ScholarPubMed
Thompson, LU, Robb, P, Serraino, M, Cheung, F 1991. Mammalian lignan production from various foods. Nutrition and Cancer 16, 43–52.CrossRefGoogle ScholarPubMed
Tsao, R, Papadopoulus, Y, Yang, R, Young, JC, McRae, K 2006. Isoflavone profiles of red clovers and their distribution in different parts harvested at different growing stages. Journal of Agricultural and Food Chemistry 54, 5797–5805.CrossRefGoogle ScholarPubMed