Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-19T02:56:41.112Z Has data issue: false hasContentIssue false
  • English
  • Français

Naringin dietary supplementation at 0.15% rates does not provide protection against sub-clinical acidosis and does not affect the responses of fattening lambs to road transportation

Published online by Cambridge University Press:  08 February 2010

Ó. López-Campos ,
R. Bodas ,
N. Prieto ,
F. J. Giráldez ,
V. Pérez  and
S. Andrés
Ó. López-Campos
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, E-24346 Grulleros, León, Spain
R. Bodas*
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, E-24346 Grulleros, León, Spain
N. Prieto
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, E-24346 Grulleros, León, Spain
F. J. Giráldez
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, E-24346 Grulleros, León, Spain
V. Pérez
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, E-24346 Grulleros, León, Spain
S. Andrés
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas, E-24346 Grulleros, León, Spain
*
† E-mail: [email protected]
Get access

Abstract

Forty Assaf fattening lambs (initial age 13 to 15 weeks) offered a diet of barley straw and a commercial concentrate were used to assess the effect of naringin (a type of citrus flavonoid with proven antioxidant, antimicrobial and anti-inflammatory properties in monogastric animals) at a dose of 1.5 g/kg per dry matteron plasma lipid peroxidation thiobarbituric acid reactive substances (TBARS), immune response, ruminal bacterial community and protection provided by the ruminal wall against subclinical acidosis. After 49 days of the experimental diets, lambs were subjected to a 4-h transportation stress period. As expected, TBARS values were significantly increased in all the lambs just after the transportation period, but no effect of naringin was observed. Although naringin lowered red blood cell count, neither the total white blood cells counts nor the production of IFN-γ were affected by naringin. No anti-inflammation activity preventing rumenitis was detected, but a clear effect on ruminal bacterial community was observed in lambs consuming naringin. Further experiments, using different doses of naringin might show health benefits of naringin supplementation in lambs, but a clear beneficial effect on health was not readily apparent in this study.

Keywords

flavonoidantioxidantimmune responsestressterminal restriction fragment length polymorphisms (T-RFLP)
Type
Full Paper
Information
animal , Volume 4 , Issue 6 , June 2010 , pp. 958 - 964
Copyright
Copyright © The Animal Consortium 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Butterweck, V, Hegger, M, Winterhoff, H 2004. Flavonoids of St. John’s Wort reduce HPA axis function in the rat. Planta Medica 70, 1008–1011.CrossRefGoogle ScholarPubMed
Carcangiu, V, Vacca, GM, Parmeggiani, A, Mura, MC, Pazzola, M, Dettori, ML, Bini, PP 2008. The effect of shearing procedures on blood levels of growth hormone, cortisol and other stress haematochemical parameters in Sarda sheep. Animal 2, 606–612.CrossRefGoogle ScholarPubMed
Castillo, M, Martín-Orúe, SM, Nafrarías, M, Manzanilla, EG, Gasa, J 2007. Changes in caecal microbiota and mucosal morphology of weaned pigs. Veterinary Microbiology 124, 239–247.CrossRefGoogle ScholarPubMed
Chung, KT, Wei, CI, Johnson, MG 1998. Are tannins a double-edged sword in biology and health? Trends in Food Science and Technology 9, 168–175.Google Scholar
D’Argenio, G, Mazzone, G, Tuccillo, C, Grandone, I, Gravina, AG, Graziani, G, Fogliano, V, Romano, M 2008. Apple polyphenol extracts prevents aspirin-induced damage to the rat gastric mucosa. The British Journal of Nutrition 100, 1228–1236.CrossRefGoogle Scholar
Community Register of Feed Additives pursuant to Regulation (EC) No 1831/2003. Appendixes 3 & 4. Annexe: List of additives. Edition 65. Released 7 December 2009. European Commission. http://ec.europa.eu/comm/food/food/animalnutrition/feedadditives/registeradditives_en.htm.Google Scholar
Farinacci, M, Colitti, M, Sgorlon, S, Stefanon, B 2008. Immunomodulatory activity of plant residues on ovine neutrophils. Veterinary Immunology and Immunopathology 126, 54–63.CrossRefGoogle ScholarPubMed
Gladine, C, Rock, E, Morand, C, Bauchart, D, Durand, D 2007. Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipoperoxidation. The British Journal of Nutrition 98, 691–701.CrossRefGoogle Scholar
Gnanamani, A, Sudha, M, Deepa, G, Sudha, M, Deivanai, K, Sadulla, S 2008. Haematological and biochemical effects of polyphenolics in animal models. Chemosphere 72, 1321–1326.CrossRefGoogle ScholarPubMed
Hamer, M 2007. The beneficial effects of tea on immune function and inflammation: a review of evidence from in vitro, animal and human research. Nutrition Research 27, 373–379.CrossRefGoogle Scholar
Hara, H, Orita, H, Hatano, S 1995. Effect of tea polyphenols on fecal flora and fecal metabolic products of pigs. The Journal of Veterinary Medical Science 57, 45–49.CrossRefGoogle ScholarPubMed
Hongoh, Y, Yuzawa, H, Ohkuma, M, Kudo, T 2003. Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment. FEMS Microbiology Letters 221, 299–304.CrossRefGoogle ScholarPubMed
Jacob, K, Periago, MJ, Böhm, V, Berrezuelo, GR 2008. Influence of lycopene and vitamin C from tomato juice on biomarkers of oxidative stress and inflammation. The British Journal of Nutrition 99, 137–146.CrossRefGoogle ScholarPubMed
Jeon, SM, Bok, SH, Jang, MK, Lee, MK, Nam, KT, Park, YB, Rhee, SJ, Choi, MS 2001. Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits. Life Sciences 69, 2855–2866.CrossRefGoogle ScholarPubMed
Kannan, G, Terrill, TH, Kouakou, B, Gazal, OS, Gelaye, S, Amoah, EA, Samake, S 2000. Transportation of goats: effects on physiological stress responses and live weight loss. Journal of Animal Science 78, 1450–1457.CrossRefGoogle ScholarPubMed
Kent, JE 1997. Stress in transported sheep. Comparative Haematology International 7, 163–166.CrossRefGoogle Scholar
Krause, KM, Oetzel, GR 2006. Understanding and preventing subacute ruminal acidosis in dairy herds: a review. Animal Feed Science and Technology 126, 215–236.CrossRefGoogle Scholar
Larraín, RE, Schaefer, DM, Richards, MP, Reed, JD 2008. Finishing steers with diets based on corn, high-tannin sorghum or a mix of both: color and lipid oxidation in beef. Meat Science 79, 656–665.CrossRefGoogle ScholarPubMed
Liu, WT, Marsh, TL, Cheng, H, Forney, LJ 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Applied Environmental Microbiology 63, 4516–4522.CrossRefGoogle ScholarPubMed
Marzo, F, Tosar, A, Santidrian, S 1990. Effect of tannic acid on the immune response of growing chicken. Jorunal of Animal Science 68, 3306–3312.CrossRefGoogle Scholar
Munday, R, Munday, JS, Munday, CM 2003. Comparative effects of mono-, di-, tri-, and tetrasulfides derived from plants of the allium family: redox cycling in vitro and hemolytic activity and phase 2 enzyme induction in vivo. Free Radical Biology and Medicine 34, 1200–1211.CrossRefGoogle ScholarPubMed
Nair, MPN, Kandaswami, C, Mahajan, S, Chadha, KC, Chawda, R, Nair, H, Kumar, N, Nair, RE, Schwartz, SA 2002. The flavonoid, quercetin, differentially regulates Th-1 (IFNγ) and Th-2 (IL4) cytokine gene expression by normal peripheral blood mononuclear cells. Biochimica et Biophysica Acta 1593, 29–36.Google Scholar
Nocek, JE, Kesler, EM 1980. Growth and rumen characteristics of holstein steers fed pelleted or conventional diets. Journal of Dairy Science 63, 249–254.Google Scholar
Ostrowska, E, Gabler, NK, Sterling, SJ, Tatham, BG, Jones, RB, Eagling, DR, Jois, M, Dunshea, FR 2004. Consumption of brown onions (Allium cepa var. cavalier and var. destiny) moderately modulates blood lipids, haematological and haemostatic variables in healthy pigs. The British Journal of Nutrition 91, 211–218.Google Scholar
Renugadevi, J, Milton Prabu, S 2009. Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology 256, 128–134.Google Scholar
SAS 1999. SAS/STAT user’s guide (Version 8). SAS Publishing, Cary, NC, USA.Google Scholar
Spencer, JPE, Abd El Mohsen, MM, Minihane, A-M, Mathers, JC 2008. Biomarkers of the intake of dietary polyphenols: strengths, limitations and application in nutrition research. The British Journal of Nutrition 99, 12–22.CrossRefGoogle ScholarPubMed
Sultan, SI, Hussein, YA 2006. Acute toxicity of Euphorbia heliscopia in rats. Pakistan Journal of Nutrition 5, 135–140.Google Scholar
Tripoli, E, La Guardia, M, Giammanco, S, Di Majo, D, Giammanco, M 2007. Citrus flavonoids: molecular structure, biological activity and nutritional properties: a review. Food Chemistry 104, 466–479.CrossRefGoogle Scholar
Yen, GC, Chen, HY, Peng, HH 2001. Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants. Food and Chemical Toxicology 39, 1045–1053.Google Scholar