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Intrauterine crowding impairs formation and growth of secondary myofibers in pigs

Published online by Cambridge University Press:  01 October 2012

C. E. Pardo ,
J. Bérard ,
M. Kreuzer  and
G. Bee
C. E. Pardo
Affiliation:
Agroscope Liebefeld-Posieux, Research Station ALP, 1725 Posieux, Switzerland ETH Zurich, Institute of Agricultural Science, Universitätstrasse 2, 8092 Zurich, Switzerland
J. Bérard
Affiliation:
Agroscope Liebefeld-Posieux, Research Station ALP, 1725 Posieux, Switzerland ETH Zurich, Institute of Agricultural Science, Universitätstrasse 2, 8092 Zurich, Switzerland
M. Kreuzer
Affiliation:
ETH Zurich, Institute of Agricultural Science, Universitätstrasse 2, 8092 Zurich, Switzerland
G. Bee*
Affiliation:
Agroscope Liebefeld-Posieux, Research Station ALP, 1725 Posieux, Switzerland
*
† E-mail: [email protected]
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Abstract

There are indications that intrauterine crowding may cause intrauterine growth retardation with the possibility of an impaired myofiber hyperplasia. The aim of the study was to confirm this by generating large differences in uterine space using sows that were unilaterally hysterectomized-ovariectomized (HO; crowded) or unilaterally oviduct ligated (OL; non-crowded). In the study, seven HO and seven OL Swiss Large White third parity sows were used. At farrowing, litter size and litter birth weight were determined. Subsequently, within each litter two male and two female progenies each with the respectively lowest (L) and highest (H) birth weight were sacrificed. Internal organs and brain were weighed, and longissimus (LM) and semitendinosus muscle (SM) samples were collected. Histological analyses were performed in both muscles using mATPase staining after preincubation at pH 4.3 and 10.2. Myosin heavy chain (MyHC) polymorphism was determined in the LM by means of SDS-PAGE. The number of piglets born alive was similar in both sow groups, but litter size expressed per uterine horn was lower (P < 0.05) in OL than HO sows. Consequently, OL progeny were markedly heavier (P < 0.01). Regardless of gender, the organs, the brain and the SM were heavier (P < 0.001) in OL and H compared with HO and L offspring, respectively. Compared with HO pigs, the SM of OL offspring tended (P < 0.1) to have more myofibers, which were of larger (P < 0.05) size. However, myofiber density appeared to be lower (P < 0.1) in the SM of OL than HO pigs. The impact of birth weight on myofiber characteristics was limited to the lower (P < 0.05) myofiber density in the SM and the larger (P < 0.01) myofiber size in the light portion of the SM of H than L offspring, whereas myofiber hyperplasia did not differ between birth weight categories. The SM, but not the LM, of male offspring had a greater (P < 0.05) myofiber density. This did not affect total SM myofiber number. The relative abundance of fetal and type I MyHC in the LM was lower (P < 0.05) and that of type II MyHC was greater (P < 0.001) in OL than HO pigs. The current data suggest that regardless of birth weight and gender, in the LM and SM of individuals born from a crowded environment, not only hyperplasia but also hypertrophy of myofibers is impaired and their maturity seems delayed.

Keywords

birth weightintrauterine crowdingmyogenesispig
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 3 , March 2013 , pp. 430 - 438
Copyright
Copyright © The Animal Consortium 2012

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References

Agroscope Liebefeld Posieux Research Station (ALP) 2012 . Fütterungsempfehlungen und Nährwerttabellen für Schweine (Feeding Recommendations and Nutrient Tables for Pigs). Retrieved April 13, 2012, from http://www.agroscope.admin.ch/futtermitteldatenbank/04835/index.html?lang=deGoogle Scholar
Bauer, R, Walter, B, Brust, P, Fuchtner, F, Zwiener, U 2003. Impact of asymmetric intrauterine growth restriction on organ function in newborn piglets. European Journal of Obstetrics Gynecology and Reproductive Biology 110, S40–S49.Google Scholar
Bee, G 2004. Effect of early gestation feeding, birth weight, and gender of progeny on muscle fiber characteristics of pigs at slaughter. Journal of Animal Science 82, 826–836.Google Scholar
Bérard, J, Bee, G 2010. Effects of dietary l-arginine supplementation to gilts during early gestation on foetal survival, growth and myofiber formation. Animal 4, 1680–1687.Google Scholar
Bérard, J, Pardo, CE, Bethaz, S, Kreuzer, M, Bee, G 2010. Intra-uterine crowding decreases average birth weight and affects muscle fiber hyperplasia in piglets. Journal of Animal Science 88, 3242–3250.Google Scholar
Christenson, RK, Leymaster, KA, Young, LD 1987. Justification of unilateral hysterectomy–ovariectomy as a model to evaluate uterine capacity in swine. Journal of Animal Science 65, 738–744.Google Scholar
Dziuk, PJ 1968. Effect of number of embryos and uterine space on embryo survival in the pig. Journal of Animal Science 27, 673–676.Google Scholar
Fenton, FR, Bazer, FW, Ulberg, LC, Schwartz, FL, Robison, OW 1972. Stage of gestation when uterine capacity limits embryo survival in gilts. Journal of Animal Science 35, 383–388.Google Scholar
Ford, SP, Vonnahme, KA, Wilson, ME 2002. Uterine capacity in the pig reflects a combination of uterine environment and conceptus genotype effects. Journal of Animal Science 80 (E. suppl. 1), E66–E73.Google Scholar
Foxcroft, GR, Bee, G, Dixon, W, Hahn, M, Harding, J, Patterson, J, Putman, T, Sarmento, S, Smit, M, Tse, W-Y, Town, SC 2007. Consequences of selection for litter size on piglet development. In Paradigms in pig science (ed. J Wiseman, MA Varley, S McOrist and B Kemp), pp. 207–229. Nottingham University Press, Nottingham, UK.Google Scholar
Gaustad-Aas, AH, Ropstad, E, Karlberg, K, Hofmo, PO, Dahl, E 2002. Oestrone sulphate measurements for the prediction of small or large litters in pigs. Acta Veterinaria Scandinavica 43, 157–164.CrossRefGoogle ScholarPubMed
Gondret, F, Lefaucheur, L, Juin, H, Louveau, I, Lebret, B 2006. Low birth weight is associated with enlarged muscle fiber area and impaired meat tenderness of the longissimus muscle in pigs. Journal of Animal Science 84, 93–103.Google Scholar
Handel, SE, Stickland, NC 1987. Muscle cellularity and birth weight. Animal Production 44, 311–317.Google Scholar
Huang, YT, Johnson, RK, Eckardt, GR 1987. Effect of unilateral hysterectomy and ovariectomy on puberty, uterine size and embryo development in swine. Journal of Animal Science 65, 1298–1305.Google Scholar
Leenhouwers, JI, van der Lende, T, Knol, EF 1999. Analysis of stillbirth in different lines of pig. Livestock Production Science 57, 243–253.Google Scholar
Lefaucheur, L, Edom, F, Ecolan, P, Butler-Browne, GS 1995. Pattern of muscle fiber type formation in the pig. Developmental Dynamics 203, 27–41.Google Scholar
Lefaucheur, L, Hoffman, R, Okamura, C, Gerrard, D, Leger, JJ, Rubinstein, N, Kelly, A 1997. Transitory expression of alpha cardiac myosin heavy chain in a subpopulation of secondary generation muscle fibers in the pig. Developmental Dynamics 210, 106–116.Google Scholar
McMillen, IC, Adams, MB, Ross, JT, Coulter, CL, Simonetta, G, Owens, JA, Robinson, JS, Edwards, LJ 2001. Fetal growth restriction: adaptations and consequences. Reproduction 122, 195–204.Google Scholar
Père, MC, Etienne, M 2000. Uterine blood flow in sows: effects of pregnancy stage and litter size. Reproduction Nutrition Development 40, 369–382.Google Scholar
Père, MC, Dourmad, JY, Etienne, M 1997. Effect of number of pig embryos in the uterus on their survival and development and on maternal metabolism. Journal of Animal Science 75, 1337–1342.CrossRefGoogle ScholarPubMed
Poore, KR, Fowden, AL 2004. The effects of birth weight and postnatal growth patterns on fat depth and plasma leptin concentrations in juvenile and adult pigs. Journal of Physiology 558, 295–304.Google Scholar
Powell, SE, Aberle, ED 1981. Skeletal muscle and adipose tissue cellularity in runt and normal birth weight swine. Journal of Animal Science 52, 748–756.Google Scholar
Rehfeldt, C, Kuhn, G 2006. Consequences of birth weight for postnatal growth performance and carcass quality in pigs as related to myogenesis. Journal of Animal Science 84, E113–E123.Google Scholar
Rehfeldt, C, Tuchscherer, A, Hartung, M, Kuhn, G 2008. A second look at the influence of birth weight on carcass and meat quality in pigs. Meat Science 78, 170–175.Google Scholar
Town, SC, Putman, CT, Turchinsky, NJ, Dixon, WT, Foxcroft, GR 2004. Number of conceptuses in utero affects porcine fetal muscle development. Reproduction 128, 443–454.Google Scholar
Tristan, F, Rivero, MA, Albors, OL, Ramis, G, Vazquez, JM, Martinez, M, Martinez, JS, Gil, F 2009. Relationship of birth weight with the size, number and proportion of fibres in the pig semitendinosus muscle. Anatomia, Histologia, Embryologia 38, 275–278.Google Scholar
Vallet, JL, Freking, BA 2006. Changes in fetal organ weights during gestation after selection for ovulation rate and uterine capacity in swine. Journal of Animal Science 84, 2338–2345.Google Scholar
van der Lende, T, van Rens, BTTM 2003. Critical periods for foetal mortality in gilts identified by analysing the length distribution of mummified foetuses and frequency of non-fresh stillborn piglets. Animal Reproduction Science 75, 141–150.Google Scholar
Wigmore, PM, Stickland, NC 1983. Muscle development in large and small pig fetuses. Journal of Anatomy 137, 235–245.Google Scholar