Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-26T01:10:22.676Z Has data issue: false hasContentIssue false
  • English
  • Français

Impacts of protein supplementation during late gestation of beef cows on maternal skeletal muscle and liver tissues metabolism

Published online by Cambridge University Press:  16 March 2020

R. C. Lopes ,
C. B. Sampaio ,
A. S. Trece ,
P. D. Teixeira ,
T. R. S. Gionbelli ,
L. R. Santos ,
T. C. Costa ,
M. S. Duarte  and
M. P. Gionbelli Open the ORCID record for M. P. Gionbelli [Opens in a new window]
R. C. Lopes
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil Trouw Nutrition, Mirassol, SP15130-000, Brazil
C. B. Sampaio
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
A. S. Trece
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
P. D. Teixeira
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
T. R. S. Gionbelli
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
L. R. Santos
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
T. C. Costa
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
M. S. Duarte
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
M. P. Gionbelli*
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
*
†E-mail: [email protected]
Get access

Abstract

Since nutritional requirements are increased at the end of gestation to meet the demands of the pregnant uterus, pregnant beef cows are susceptible to mobilization of body reserves (mainly fat and amino acids (AAs)) and to alter the metabolism of nutrients in the liver and muscle to support such demands. The objective of this study was to evaluate the effect of CP supplementation on maternal nutrient metabolism in the late gestation of beef cows grazing a low-quality pasture. Forty-three pregnant Nellore cows gestating male fetuses (average age = 6 years; average weight = 544 kg) at 193 ± 30 (mean ± SD) days (d) of gestation were divided into eight groups (experimental units, with four to five cows each). Treatments were (1) control (CON, n = 4): pasture-based (PB) diet without CP supplementation and (2) supplemented (SUP, n = 4): PB diet daily supplemented with 2 g/kg of BW of a 43.5% CP supplement. Liver and skeletal muscle biopsies were performed at 265 days of gestation and samples were collected for mRNA expression. On day 280 of gestation, blood samples were collected to assess plasma levels of AA. The CON-fed cows tended to have greater (P = 0.057) total circulating AA than SUP-fed cows. The circulating glycogenic AA was greater (P = 0.035) in CON than in SUP cows. CON cows was greater for histidine (P = 0.015), methionine (P = 0.007) and alanine (P = 0.036) than SUP cows. The CON- and SUP-fed showed no differences for gluconeogenesis, fatty acid transport and signaling axis markers in the liver. The mRNA expression of markers for skeletal muscle synthesis, p7056k (P = 0.060) and GSK3B (P = 0.096), tended to be greater in cows from CON than SUP group. No differences were found for mRNA expression of markers for skeletal muscle degradation. We conclude that CP supplementation to CP-restricted late-pregnant beef cows reduces the maternal tissue mobilization and changes the profile of plasma circulating AA and the mRNA expression of markers for the synthesis of skeletal muscle tissue.

Keywords

amino acidsfetal programminggluconeogenesismaternal nutritiontissue mobilization
Type
Research Article
Information
animal , Volume 14 , Issue 9 , September 2020 , pp. 1867 - 1875
Copyright
© The Animal Consortium 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arrigoni, MB, Alves Júnior, A, Dias, PMA, Martins, CL, Cervieri, RC, Silveira, AC, Oliveira, HN and Chardulo, LAL 2004. Desempenho, fibras musculares e carne de bovinos jovens de três grupos genéticos. Pesquisa Agropecuaria Brasileira 39, 1033–1039.CrossRefGoogle Scholar
Bell, AW 1995. Use of ruminants to study regulation of nutrient partitioning during pregnancy and lactation. In Animal science research and development: moving toward a new century (ed. Ivan, M), pp. 41–62. Minister of Supply and Services, Ottawa, Canada.Google Scholar
Bell, AW, Burhans, WS and Overton, TR 2000. Protein nutrition in late pregnancy, maternal protein reserves and lactation performance in dairy cows. Proceedings of the Nutrition Society 59, 119–126.CrossRefGoogle ScholarPubMed
Bell, AW and Ehrhardt, RA 2000. Regulation of macronutrient partitioning between maternal and conceptus tissues in the pregnant ruminant. In Ruminant physiology: digestion, metabolism, growth and reproduction (ed. Cronjé, Pb), pp. 275–293. CABI Publishing, New York, NY, USA.CrossRefGoogle Scholar
Bell, AW, Ferrell, CL and Freetly, HC 2005. Pregnancy and fetal metabolism. In Quantitative aspects of rumiant digestion and metabolism (ed. Dijkstra, J, Forbes, J and France, JM), pp. 523–550. CABI Publishing, Wallingford, UK.CrossRefGoogle Scholar
Bell, AW, Slepetis, R and Ehrhardt, UA 1995. Growth and accretion of energy and protein in the gravid uterus during late pregnancy in Holstein cows. Journal of Dairy Science 78, 1954–1961.CrossRefGoogle ScholarPubMed
Bohnert, DW, Stalker, LA, Mills, RR, Nyman, A, Falck, SJ and Cooke, RF 2013. Late gestation supplementation of beef cows differing in body condition score: effects on cow and calf performance. Journal of Animal Science 91, 5485–5491.CrossRefGoogle ScholarPubMed
Crookenden, MA, Mandok, KS, Grala, TM, Phyn, CVC, Kay, JK, Greenwood, SL and Roche, JR 2015. Source of metabolizable energy affects gene transcription in metabolic pathways in adipose and liver tissue of nonlactating, pregnant dairy cows. Journal of Animal Science 93, 685–698.CrossRefGoogle ScholarPubMed
Detmann, E, Paulino, MF, Mantovani, HC, Valadares Filho, SC, Sampaio, CB, Souza, MA, Lazzarini, Í and Detmann, KSC 2009. Parameterization of ruminal fibre degradation in low-quality tropical forage using Michaelis–Menten kinetics. Livestock Science 126, 136–146.CrossRefGoogle Scholar
Detmann, E, Souza, MA, Valadares Filho, SC, Queiroz, AC, Berchielli, TT, Saliba, EOS, Cabral, LS, Pina, DS, Ladeira, MM and Azevedo, JAG 2012. In Métodos para análise de alimentos. 1st edition. Suprema, Visconde do Rio Branco, Minas Gerais, Brazil.Google Scholar
Doelman, J, Cao, H, Purdie, NG, Kim, JJM, Swanson, KC, Osborne, VR, Tey, J, Ali, A, Feng, Z, Karrow, NA and Cant, JP 2012. Transcript profiling of the ruminant liver indicates a unique program of transcriptional regulation of ketogenic enzymes during food restriction. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 7, 303–310.Google ScholarPubMed
Drackley, JK, Overton, TR and Douglas, GN 2001. Adaptations of glucose and long-chain fatty acid metabolism in liver of dairy cows during the periparturient period. Journal of Dairy Science 84, E100–E112.CrossRefGoogle Scholar
Duarte, MS, Gionbelli, MP, Paulino, PVR, Serão, NVL, Martins, TS, Totaro, PIS, Neves, CA, Valadares Filho, SC, Dodson, MV, Zhu, M and Du, M 2013. Effects of maternal nutrition on development of gastrointestinal tract of bovine fetus at different stages of gestation. Livestock Science 153, 60–65.CrossRefGoogle Scholar
Engle, TE, Spears, JW, Xi, L and Edens, FW 2000. Dietary copper effects on lipid metabolism and circulating catecholamine concentrations in finishing steers. Journal of Animal Science 78, 2737–2744.CrossRefGoogle ScholarPubMed
Gionbelli, MP, Duarte, MS, Valadares Filho, SC, Detmann, E, Chizzotti, ML, Rodrigues, FC, Zanetti, D, Gionbelli, TRS and Machado, MG 2015. Achieving body weight adjustments for feeding status and pregnant or non-pregnant condition in beef cows. PLoS ONE 10, e0112111.CrossRefGoogle ScholarPubMed
Kalhan, SC and Marczewski, SE 2012. Methionine, homocysteine, one carbon metabolism and fetal growth. Reviews in Endocrine and Metabolic Disorders 13, 109–119.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402–408.CrossRefGoogle Scholar
Lopes, RC 2017. Protein supplementation in late gestation for beef cows affects maternal skeletal muscle gene expression and plasma circulating amino acids. Master dissertation, Universidade Federal de Lavras, Lavras, MG, Brazil.Google Scholar
McNeill, DM, Slepetis, R, Ehrhardt, RA, Smith, DM and Bell, AW 1997. Protein Requirements of sheep in late pregnancy: partitioning of nitrogen between gravid uterus and maternal tissues. Journal of Animal Science 75, 809–816.CrossRefGoogle ScholarPubMed
Meijer, AJ 2003. Amino acids as regulators and components of nonproteinogenic pathways. The Journal of Nutrition 133, 2057S–2062S.CrossRefGoogle ScholarPubMed
Mordhorst, BR, Zimprich, CA, Camacho, LE, Bauer, ML and Vonnahme, KA 2017. Supplementation of distiller’s grains during late gestation in beef cows consuming low quality forage decreases uterine, but not mammary, blood flow. Journal of Animal Physiology and Animal Nutrition 101, e154–e164.CrossRefGoogle Scholar
Nicholson, MJ and Butterworth, MH 1986. A guide to condition scoring of zebu cattle. International Livestock Research Institute, Addis Ababa, Ethiopia.Google Scholar
Pearson, EG and Craig, AM 1980. The diagnosis of liver disease in equine and food animals. Modern Veterinary Practice 61, 233–237.Google Scholar
Stalker, LA, Ciminski, LA, Adams, DC, Klopfenstein, TJ and Clark, RT 2007. Effects of weaning date and prepartum protein supplementation on cow performance and calf growth. Rangeland Ecology & Management 60, 578–587.CrossRefGoogle Scholar
Vonnahme, KA, Lemley, CO, Caton, JS and Meyer, AM 2015. Impacts of maternal nutrition on vascularity of nutrient transferring tissues during gestation and lactation. Nutrients 7, 3497–3523.CrossRefGoogle ScholarPubMed
Winterholler, SJ, McMurphy, CP, Mourer, GL, Krehbiel, CR, Horn, GW and Lalman, DL 2012. Supplementation of dried distillers grains with solubles to beef cows consuming low-quality forage during late gestation and early lactation. Journal of Animal Science 90, 2014–2025.CrossRefGoogle ScholarPubMed
Wood, KM, Awda, BJ, Fitzsimmons, C, Miller, SP, McBride, BW and Swanson, KC 2013. Influence of pregnancy in mid-to-late gestation on circulating metabolites, visceral organ mass, and abundance of proteins relating to energy metabolism in mature beef cows. Journal of Animal Science 91, 5775–5784.CrossRefGoogle ScholarPubMed
Wu, G 2009. Amino acids: metabolism, functions, and nutrition. Amino Acids 37, 1–17.CrossRefGoogle ScholarPubMed
Zhou, Z, Loor, JJ, Piccioli-Cappelli, F, Librandi, F, Lobley, GE and Trevisi, E 2016. Circulating amino acids in blood plasma during the peripartal period in dairy cows with different liver functionality index. Journal of Dairy Science 99, 2257–2267.CrossRefGoogle ScholarPubMed