Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-19T02:59:58.445Z Has data issue: false hasContentIssue false
  • English
  • Français

Feed intake, milk production and composition of crossbred cows fed with insect-protected Bollgard II® cottonseed containing Cry1Ac and Cry2Ab proteins

Published online by Cambridge University Press:  03 June 2011

K. K. Singhal ,
A. K. Tyagi ,
Y. S. Rajput ,
M. Singh ,
H. Kaur ,
T. Perez  and
G. F. Hartnell
K. K. Singhal
Affiliation:
National Dairy Research Institute, Karnal 132001, Haryana, India
A. K. Tyagi
Affiliation:
National Dairy Research Institute, Karnal 132001, Haryana, India
Y. S. Rajput
Affiliation:
National Dairy Research Institute, Karnal 132001, Haryana, India
M. Singh*
Affiliation:
National Dairy Research Institute, Karnal 132001, Haryana, India
H. Kaur
Affiliation:
National Dairy Research Institute, Karnal 132001, Haryana, India
T. Perez
Affiliation:
Monsanto Company, St. Louis 63167, MO, USA
G. F. Hartnell
Affiliation:
Monsanto Company, St. Louis 63167, MO, USA
*
†E-mail: [email protected]
Get access

Abstract

Twenty crossbred lactating multiparous cows were used in a 28-day study to compare dry matter intake (DMI), milk yield, milk composition and Bacillus thuringiensis (Bt) protein concentrations in plasma when fed diets containing Bollgard II® cottonseed (BGII) or a control non-genetically modified isogenic cottonseed (CON). Bollgard II cottonseed contains the Cry1Ac and Cry2Ab insecticidal proteins that protect cotton plants from feeding damage caused by certain lepidopteran insects. Cows were assigned randomly to the BGII or CON treatments after a 2-week adjustment period. Cows consumed a concentrate containing 40% crushed cottonseed according to milk yield and green maize forage ad libitum. All cows received the same diet but with different crushed cottonseed sources. Cottonseed was included to provide approximately 2.9 kg per cow daily (dry matter basis). The ingredient composition of the concentrate was 40% crushed cottonseed, 15% groundnut cake, 20% corn, 22% wheat bran, 1% salt and 2% mineral mixture. Milk and blood plasma were analyzed for Cry1Ac and Cry2Ab proteins. DMI, BW, milk yield and milk components did not differ between cows on the BGII and CON treatments. Although milk yield and milk fat percentage were not affected by treatment, 4% fat-corrected milk (FCM) production and FCM/kg DMI for cows on the BGII treatment (14.0 kg/cow per day, 1.12 kg/kg) were significantly improved compared with cows on the CON treatment (12.1 kg/cow per day, 0.97 kg/kg). Gossypol contents in BGII cottonseed and conventional cottonseed were similar. Cry1Ac and Cry2Ab2 proteins in Bollgard II cottonseed were 5.53 and 150.8 μg/g, respectively, and were not detected in the milk or plasma samples. The findings suggested that Bollgard II cottonseed can replace conventional cottonseed in dairy cattle diets with no adverse effects on performance and milk composition.

Keywords

dairy cowcottonseedgenetically modifiedmilk yield and composition
Type
Full Paper
Information
animal , Volume 5 , Issue 11 , 26 September 2011 , pp. 1769 - 1773
Copyright
Copyright © The Animal Consortium 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Association of Official and Analytical Chemists (AOAC) 1990. Official methods of analysis, 15th edition. AOAC, Arlington, VA, USA.Google Scholar
Bates, SL, Zhao, JZ, Roush, RT, Shelton, AM 2005. Insect resistance management in GM crops: past, present and future. Nature Biotechnology 23, 57–62.CrossRefGoogle ScholarPubMed
Betz, FS, Hammond, BG, Fuchs, RL 2000. Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Toxicology and Pharmacology 32, 156–173.Google ScholarPubMed
Bureau of Indian Standards (BIS) 1975. Specifications for compounded feed for cattle. IS: 7874, part I, Indian Standard Institute, Mank Bhavan, New Delhi, India.Google Scholar
Castillo, AR, Gallardo, MR, Maciel, JM, Giordano, GA, Conti, MC, Gaggiotti, O, Quaino, CG, Hartnell, GF 2004. Effects of feeding rations with genetically modified whole cottonseed to lactating dairy cows. Journal of Dairy Science 87, 1778–1785.CrossRefGoogle Scholar
Goering, HK, Van Soest, PJ 1967. Forage fibre analyses: apparatus, reagents, procedures, and some applications, Agriculture handbook no. 379. ARS-USDA, Washington, DC, USA.Google Scholar
Indian Standards Institution (ISI) 1958. IS: 1224. Determination of fat in whole milk, evaporated milk, separated milk, skim milk, butter milk and cream by Gerber method. I.S.I., Manak Bhawan, New Delhi, India.Google Scholar
James, C 2008. Global status of commercialised biotech/GM crops. ISAAA Brief 39. ISAAA, Ithaca, New York, USA.Google Scholar
Mertens, DR 1985. Effect of fibre on feed quality for dairy cows. Proceedings of the 46th Minnesota Nutrition Conference, St. Paul, University of Minnesota, USA, pp. 209–224.Google Scholar
Mohanta, RK, Singhal, KK, Tyagi, AK, Rajput, YS, Prashad, S 2009. Nutritional evaluation of transgenic cottonseed in the ration of lactating dairy cows. Tropical Animal Health and Production 42, 431–438.CrossRefGoogle ScholarPubMed
Mujahid, AM, Abdullah, AR, Barque, K, Gilani, AH 2000. Nutritional value of cottonseeds and its derived products. II Free gossypol, available lysine and in vitro protein degradability. Asian Australasian Journal of Animal Science 13, 348–355.CrossRefGoogle Scholar
Nazem, AM, Zab, MH, Tahlaury, MR, Mohmoud, MR 1998. Detection of apparently normal milking screening and confirmatory method. VIII Scientific Congress (15–17 November), Faculty of Veterinary Medicine, Assikt University, pp. 1–12.Google Scholar
National Research Council (NRC) 2001. Nutrient requirements of dairy cattle NRC, 7th revised edition. National Academy Press, Washington, DC.Google Scholar
SAS 1985. SAS user's guide: statistics, 5th edition. SAS Institute Incorporation, Cary, NC, USA.Google Scholar
Sen, KC, Ray, SN 1977. Nutritive value of Indian cattle feeds and feeding of animals. ICAR, Krishi Bhavan, New Delhi, India.Google Scholar
Senthil, K, Singhal, KK 2004. Chemical composition and nutritional evaluation of transgenic cottonseed for ruminants. Indian Journal of Animal Science 74, 868–871.Google Scholar
Singh, M, Ludri, RS 2001. Somatic cell count in Murrah buffaloes during different stages of lactation, parity and season. Asian-Australasian Journal of Animal Science 14, 189–192.CrossRefGoogle Scholar
Singhal, KK, Kumar, S, Tyagi, AK, Rajput, YS 2006. Evaluation of Bt cottonseed as a protein supplement in the ration of lactating dairy cows. Indian Journal of Animal Science 76, 532–537.Google Scholar
Smith, FH 1968. Estimation of free gossypol in cottonseed meal and cottonseed meats: modified method. Journal of American Oil Chemical Society 45, 903.CrossRefGoogle Scholar