Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-29T14:10:18.208Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of swine fertilisation medium (SFM) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extenders

Published online by Cambridge University Press:  28 October 2008

P. Fantinati ,
A. Zannoni ,
C. Bernardini ,
M. Forni ,
A. Tattini ,
E. Seren  and
M. L. Bacci
P. Fantinati
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
A. Zannoni
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
C. Bernardini
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
M. Forni
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
A. Tattini
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
E. Seren
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
M. L. Bacci*
Affiliation:
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
*
E-mail: [email protected]
Get access

Abstract

In pig production, artificial insemination is widely carried out and the use of fresh diluted semen is predominant. For this reason, there are increasing interests in developing new extenders and in establishing the optimal storage conditions for diluted spermatozoa. In the last few decades, we utilised a homemade diluent (swine fertilisation medium (SFM)) for spermatozoa manipulation and biotechnological application as the production of transgenic pigs utilising the sperm-mediated gene transfer technique. The purpose of the present study is therefore to analyse the ability of SFM, in comparison to four commercial extenders, in preserving the quality of diluted boar semen stored at 16.5°C till 15 days. We utilised some of the main predictive tests as objectively measured motility, acrosome and sperm membrane integrity, high mitochondrial membrane potential and pH. Based on our in vitro study, SFM could be declared as a good long-term extender, able to preserve spermatozoa quality as well as Androhep Enduraguard for up to 6 to 9 days and more.

Keywords

boarspermatozoa qualityextendersemen storage
Type
Full Paper
Information
animal , Volume 3 , Issue 2 , February 2009 , pp. 269 - 274
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alvarez, JG, Storey, BT 1983. Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biology of Reproduction 29, 548–555.CrossRefGoogle ScholarPubMed
Bailey, JL, Bilodeau, JF, Cornier, N 2000. Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. Journal of Andrology 21, 1–7.CrossRefGoogle ScholarPubMed
Bamba, K, Sone, M 1981. Factors affecting the quality of boar semen stored by means of dialysis. Journal of Reproduction and Fertility 62, 193–197.CrossRefGoogle ScholarPubMed
Cerolini, S, Maldjian, A, Surai, P, Noble, R 2000. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Animal Reproduction Science 58, 99–111.CrossRefGoogle ScholarPubMed
Fantinati, P, Zannoni, A, Bernardini, C, Webster, N, Lavitrano, M, Forni, M, Seren, E, Bacci, ML 2005. Laparoscopic insemination technique with low numbers of spermatozoa in superovulated prepuberal gilts for biotechnological application. Theriogenology 63, 806–817.CrossRefGoogle ScholarPubMed
Flowers, WL 1997. Management of boars for efficient semen production. Journal of Reproduction and Fertility 52 (suppl.), 67–78.Google Scholar
Fraser, L, Strzezek, J 2004. The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C. Folia Histochemica et Cytobiologica 42, 49–55.Google Scholar
Gadea, J 2003. Semen extenders used in the artificial insemination of swine. A review. Spanish Journal of Agricultural Research 1, 17–27.CrossRefGoogle Scholar
Gallon, F, Marchetti, C, Jouy, N, Marchetti, P 2006. The functionality of mitochondria differentiates human spermatozoa with high and low fertilizing capability. Fertility and Sterility 86, 1526–1530.CrossRefGoogle ScholarPubMed
Garner, DL, Thomas, CA, Joerg, HW, Dejarnette, JM, Marshall, CE 1997. Fluorometric assessment of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biology of Reproduction 57, 1401–1406.CrossRefGoogle ScholarPubMed
Gerrits, RJ, Lunney, JK, Johnson, LA, Pursel, VG, Kraeling, RR, Rohrer, GA, Dobrinsky, JR 2005. Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology 63, 283–299.CrossRefGoogle ScholarPubMed
Harrison, RA, Dott, HM, Foster, GC 1982. Bovine serum albumin, sperm motility, and the dilution effect. Journal of Experimental Zoology 222, 81–88.CrossRefGoogle ScholarPubMed
Holt, C, Holt, WV, Moore, HDM, Reed, HCB, Curnokz, RM 1997. Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. Journal of Andrology 18, 312–323.CrossRefGoogle ScholarPubMed
Huo, LJ, Ma, XH, Yang, ZM 2002. Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology 58, 1349–1360.CrossRefGoogle ScholarPubMed
Johnson, LA, Aalbers, JG, Groothe, HJG 1988. Artificial insemination of swine: fecundity of boar semen stored in Beltsville Thawing Solution (BTS), modified Modena (MM), or MR-A and inseminated on one, three and four days after collection. Reproduction in Domestic Animals 23, 49–55.CrossRefGoogle Scholar
Johnson, LA, Weitze, KF, Fiser, P, Maxwell, WMC 2000. Storage of boar semen. Animal Reproduction Science 62, 143–172.CrossRefGoogle ScholarPubMed
Jones, JM, Bavister, BD 2000. Acidification of intracellular pH in bovine spermatozoa suppresses motility and extend viable life. Journal of Andrology 21, 616–624.CrossRefGoogle ScholarPubMed
Lavitrano, M, Bacci, ML, Forni, M, Lazzereschi, D, Di Stefano, C, Fioretti, D, Giancotti, P, Marfé, G, Pucci, L, Renzi, L, Wang, H, Stoppacciaro, A, Stassi, G, Sargiacomo, M, Sinibaldi, P, Turchi, V, Giovannoni, R, Della Casa, G, Seren, E, Rossi, G 2002. Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. Proceedings of the National Academy of Sciences of the United States of America 99, 14230–14235.CrossRefGoogle ScholarPubMed
Lavitrano, M, Forni, M, Bacci, ML, Di Stefano, C, Varzi, V, Wang, H, Seren, E 2003. Sperm mediated gene transfer in pig: selection of donor boars and optimization of DNA uptake. Molecular Reproduction and Development 64, 284–291.CrossRefGoogle ScholarPubMed
Levis, DG 2000. Liquid boar semen production: current extender technology and where do we go from here! In Boar Semen Preservation IV (ed. LA Johnson and HD Guthrie), Proceedings of the IV International Conference on Boar Semen Preservation, pp. 121–128. Allen Press Inc., Lawrence, KS, USA.Google Scholar
Manzini, S, Vargiolu, A, Stehle, IM, Bacci, ML, Cerrito, MG, Giovannoni, R, Zannoni, A, Bianco, MR, Forni, M, Donini, P, Papa, M, Lipps, HJ, Lavitrano, M 2006. Genetically modified pigs produced with a nonviral episomal vector. Proceedings of the National Academy of Sciences of the United States of America 103, 17672–17677.CrossRefGoogle ScholarPubMed
Paulenz, H, Kommisrud, E, Hofmo, PO 2000. Effect of long-term storage at different temperatures on the quality of liquid boar semen. Reproduction in Domestic Animals 35, 83–87.CrossRefGoogle Scholar
Rodriguez-Martinez, H, Ekstedt, E, Einarsson, S 1990. Acidification of epididymal fluid in the boar. International Journal of Andrology 13, 238–243.CrossRefGoogle ScholarPubMed
Thacker, BJ, Larsen, RE, Joo, HS, Leman, AD 1984. Swine disease transmissible with artificial insemination. Journal of the American Veterinary Medical Association 185, 511–516.Google ScholarPubMed
Turba, ME, Fantinati, P, Bernardini, C, Gentilini, F, Bacci, ML, Forni, M 2007. Relationships between innovative and traditional parameters to investigate semen quality in pigs. Animal Reproduction Science 99, 72–81.CrossRefGoogle ScholarPubMed
Verstegen, J, Iguer-Ouada, M, Onclin, K 2002. Computer assisted semen analyzer in andrology research and veterinary practice. Theriogenology 57, 149–179.CrossRefGoogle ScholarPubMed
Vishwanath, R 2003. Artificial insemination: the state of the art. Theriogenology 59, 571–584.CrossRefGoogle ScholarPubMed
Vyt, P, Maes, D, Dejonckheere, E, Castryck, F, Van Soom, A 2004. Comparative study on five different commercial extenders for boar semen. Reproduction in Domestic Animals 39, 8–12.CrossRefGoogle ScholarPubMed
Waberski, D, Weitze, KF, Rath, D, Sallman, HP 1989. Effect of bovine serum albumin and zwitterionic buffers on stored liquid boar semen. Zuchthygiene 24, 128–133.CrossRefGoogle Scholar
Waberski, D, Meding, S, Dirksen, G, Weitze, KF, Leiding, C, Hahn, R 1994. Fertility of long-term-stored boar semen: influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen. Animal Reproduction Science 36, 145–151.CrossRefGoogle Scholar
Webster, NL, Forni, M, Bacci, ML, Giovannoni, R, Razzini, R, Fantinati, P, Zannoni, A, Fusetti, L, Dalprà, L, Bianco, MR, Papa, M, Seren, E, Sandrin, MS, Mc Kenzie, IF, Lavitrano, M 2005. Multi-transgenic pigs expressing three fluorescent proteins produced with high efficiency by sperm mediated gene transfer. Molecular Reproduction and Development 72, 68–76.CrossRefGoogle ScholarPubMed
Weitze, KF 1991. Long-term storage of extended boar semen. In Proceedings of the 2nd International Conference on Boar Semen Preservation (ed. LA Johnson and D Rath), pp. 231–253. Paul Parey Scientific Publishers, Berlin, Germany.Google Scholar
Supplementary material: File

Fantinati Supplementary Material

Appendix.doc

Download Fantinati Supplementary Material(File)
File 148 KB