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Effects of ochratoxin A on membrane phospholipids of the intestine of broiler chickens, practical consequences

Published online by Cambridge University Press:  30 October 2019

I. El Cafsi*
Affiliation:
Institute Pasteur of Tunis – Laboratory of Veterinary Epidemiology and Microbiology, University Tunis-El Manar, 1002 Tunis, Tunisia Faculty of Sciences of Tunis, Unit of Physiology and Aquatic Enviroment, University Tunis-El Manar, 2092 Tunis, Tunisia
S. Bjeoui
Affiliation:
Faculty of Sciences of Tunis, Unit of Physiology and Aquatic Enviroment, University Tunis-El Manar, 2092 Tunis, Tunisia
I. Rabeh
Affiliation:
Faculty of Sciences of Tunis, Unit of Physiology and Aquatic Enviroment, University Tunis-El Manar, 2092 Tunis, Tunisia
S. Nechi
Affiliation:
Mohamed Tahar Maamouri Hospital, Anatomy and Cytology Service, Road Mrezka 8000, Nabeul, Tunisia
E. Chelbi
Affiliation:
Mohamed Tahar Maamouri Hospital, Anatomy and Cytology Service, Road Mrezka 8000, Nabeul, Tunisia
M. El Cafsi
Affiliation:
Faculty of Sciences of Tunis, Unit of Physiology and Aquatic Enviroment, University Tunis-El Manar, 2092 Tunis, Tunisia
A. Ghram
Affiliation:
Institute Pasteur of Tunis – Laboratory of Veterinary Epidemiology and Microbiology, University Tunis-El Manar, 1002 Tunis, Tunisia
*
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Abstract

Ochratoxin A (OTA) is a mycotoxin produced by various species of Aspergillus and Penicillium. Ochratoxin A was classified as a group 2B carcinogen and is one of the major intestinal pathogenic mycotoxins. One of the most frequent modes of intoxication is consumption of contaminated food with mycotoxins. Feed represents the major cost and has a direct impact on the economical viability of broiler’s production system, since it must contain the necessary elements that allow the animal to express the maximum genetic potential while providing its nutritional requirements. Thus, the animal has to digest the feed and absorb its nutrients, which is in direct correlation with the gastrointestinal tract, especially the small intestine and the development of the mucosal surface area. Once ingested, OTA is absorbed by passive diffusion, mainly the jejunum. Ochratoxin A’s presence affects lipid membranes and could lead to the degradation of their normal structure and functionality. All of these effects contribute to the development of malabsorption. It was very interesting to study the effect of OTA on the layer of phospholipids of the bowel. The experimental group received OTA (0.05 to mg/kg BW) through an intra-peritoneal injection, every other day for 21 days. We noted that feed conversion ratio and average daily gain were reduced. Histological studies showed important alterations at the level of the mucosal membrane of the intestine (villosities, crypts) following intra-peritoneal administration of the mycotoxin. Thinning and enlargement at the base of the villosities, hyperplasia and crypts in irregular forms, blunting and denudation were observed through the examination of intestinal morphology. Biochemical studies, such as total lipid and phospholipid compositions, allowed us to have more detailed results. All identified mucosal phospholipids were modified, particularly the phosphatidylcholine (PC) and the phosphatidylethanolamine (PE) in the jejunum mucosa. In fact, there was a decrease by 55.81% for PC, 56.66% for PE, while a significant increase by 32.91% was noted for phosphatidylserine in the jejunum. It was very interesting to study the effect of OTA on the phospholipids layer of the bowel, as the mucous membrane of the small intestine represents the main site of absorption and transformation of nutriments. To avoid such disturbances and prevent the effects of the OTA, precautions must be taken to inhibit mold growth at the level of the feed manufactory units. Phosphatidylcholine and PE administrations may represent an option that could allow reestablishment of phospholipid equilibrium in the intestine.

Type
Research Article
Copyright
© The Animal Consortium 2019

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References

Al-Anati, L and Petzinger, E 2006. Immunotoxic activity of ochratoxin A. Journal Veterinary Pharmacology and Therapeutics 29, 7990.CrossRefGoogle ScholarPubMed
Alessandri, JM, Arfi, TS and Thieulin, C 1990. La muqueuse de l’intestin grêle: évolution de la composition en lipides cellulaires au cours de la différenciation entérocytaire et de la maturation postnatale. Reproduction Nutrition Development 30, 551576.CrossRefGoogle Scholar
Blem, CR 1976. Patterns of lipid storage and utilization in birds. Journal Integrative and Comparative Biology 16, 671684.Google Scholar
Bremer, J and Greenberg, DM 1960. Biosynthesis of choline in vitro. Journal Biochimica et Biophysica Acta 37, 173175.CrossRefGoogle ScholarPubMed
Cecchi, G, Basini, S and Castano, C 1985. Méthanolyse rapide des huiles en solvent. Revue Française des Corps Gras 32, 163164.Google Scholar
Chang, C-F, John, A, Pat, D and Hamilton, B 1981. Experimental Ochratoxicosis in Turkey Poults. Journal Poultry Science 60, 114119.CrossRefGoogle ScholarPubMed
Cleynen, I, Vazeille, E, Artieda, M, Verspaget, H W, Szczypiorska, M, Bringer, MA and Darfeuille-Michaud, A 2014. Genetic and microbial factors modulating the ubiquitin proteasome system in inflammatory bowel disease. Journal Gut 63, 12651274.CrossRefGoogle ScholarPubMed
Dennis, EA and Kennedy, EP 1972. Intracellular sites of lipid synthesis and the biogenesis of mitochondria. Journal of Lipid Research 13, 263267.Google ScholarPubMed
Duarte, SC, Lino, CM and Pena, A 2011. Ochratoxin A in feed of food-producing animals: an undesirable mycotoxin with health and performance effects. Journal Veterinary Microbiology 154, 113.CrossRefGoogle ScholarPubMed
Fink-Gremmels, J 2008. The role of mycotoxins in the health and performance of dairy cows. Veterinary Journal 176, 8492.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M and Sloane-Stanley, GA 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497509.Google ScholarPubMed
Food and Agriculture Organization of the United Nations (FAO) 2004. Worldwide regulations for mycotoxins in food and feed in 2003. FAO food and nutrition, p. 81. FAO, Rome, Italy.Google Scholar
Friedman, JR, Kannan, M, Toulmay, A, Jan, CH, Weissman, JS, Prinz, WA and Nunnari, J 2018. Lipid homeostasis is maintained by dual targeting of the mitochondrial pe biosynthesis enzyme to the ER. Developmental Cell 44, 261270.CrossRefGoogle ScholarPubMed
Gentles, A, Smith, EE, Kubena, LF, Duffus, E, Johnson, P, Thomson, J, Harvey, RB and Edrington, TS 1999. Toxicological evaluations of cyclopiazonic acid and ochratoxin A in broilers. Journal Poultry Science 78, 13801384.CrossRefGoogle ScholarPubMed
Goodlad, RA, Levi, S, Lee, CY, Mandir, N, Hodgson, H and Wright, NA 1991. Morphometry and cell proliferation in endoscopic biopsies: evaluation of a technique. Gastroenterology 101, 12351241.CrossRefGoogle ScholarPubMed
Hanif, NQ 2016. Ochratoxicosis in monograstric animals – a review. Journal of Bioresource Management 3, 124.Google Scholar
Institut National de la Recherche Agronomique 1992. Nutrition et alimentation des volailles. INRA, Paris, France.Google Scholar
Kennedy, EP and Weiss, SB 1956. The function of cytidine coenzymes in the biosynthesis of phospholipides. The Journal of Biological Chemistry 222, 193214.Google ScholarPubMed
Kruskal, WH and Wallis, WA 1952. Use of ranks in one-criterion variance analysis. Journal of the American Statistical Association 47, 583621.CrossRefGoogle Scholar
Kuge, O, Nishijima, M and Akamatsu, Y 1986. Phosphatidylserine biosynthesis in cultured Chinese hamster ovary cells. III. Genetic evidence for utilization of phosphatidylcholine and phosphatidylethanolamine as precursors. Journal of Biological Chemistry 261, 57955798.Google ScholarPubMed
Levene, H 1960. Robust tests for equality of variances. In Contributions to probability ans statics: essays in honor of Harold Hotelling (ed. Olkin, I, Ghurye, SG, Huffing, W, Madow, WG and Mann, HB), pp. 278292. Stanford University Press, Stanford, CA.Google Scholar
Liew, W-P-P and Mohd-Redzwan, S 2018. Mycotoxin: its impact on gut health and microbiota. Frontiers in Cellular and Infection Microbiology 8, 60.CrossRefGoogle ScholarPubMed
Maresca, M, Mahfoud, R, Pfohl-Leszkowicz, A and Fantini, J 2001. The mycotoxin ochratoxin A alters intestinal barrier and absorption functions but has no effect on chloride secretion. Toxicology and Applied Pharmacology Journal 176, 5463.CrossRefGoogle ScholarPubMed
Marquardt, RR 1996. Effects of molds and their toxins on livestock performance: a western Canadian perspective. Animal Feed Science and Technology 58, 7789.CrossRefGoogle Scholar
McLaughlin, J, Padfield, PJ, Burt, JPH and O’Neill, CA 2004. Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms. American Journal of Physiologie-Cell Physiol 287, C14121417.CrossRefGoogle ScholarPubMed
Moura, M, Machado, C, Porfírio, L and Freire, R 2004. Effects of ochratoxin A on broiler leukocytes. RevistaBrasileira de CiênciaAvícola 6, 187190.Google Scholar
Olsen, RE and Henderson, RJ 1989. The rapid analysis of neutral and polar marine lipids using double-development HPTLC and scanning densitometry. Journal of Experimental Marine Biology and Ecology 129, 189197.CrossRefGoogle Scholar
Piotrowska, M, Ślizewska, K, Nowak, A, Zielonka, Ł, Zakowska, Z, Gajecka, M and Gajecki, M 2014. The effect of experimental Fusariummycotoxicosis on microbiota diversity in porcine ascending colon contents. Toxins 6, 20642081.CrossRefGoogle ScholarPubMed
Prior, MG, Sisodia, CS, O’Neil, JB 1976. Acute oral ochratoxicosis in day-old white leghorns, Turkeys and Japanese quail. Journal Poultry Science 55, 786790.CrossRefGoogle ScholarPubMed
Ruan, D, Wang, WC, Lin, CX, Fouad, AM, Chen, W, Xia, WG, Yang, L 2018. Effects of curcumin on performance, antioxidation, intestinal barrier and mitochondrial function in duck fed corn contaminated with ochratoxin A. Animal 12, 111.Google Scholar
Shapiro, SS and Wilk, MB 1965. An analysis of variance test for normality (complete samples). Biometrika 52, 591611.CrossRefGoogle Scholar
Shewfelt, RL, Mcdonald, RE and Hultin, HO 1981. Effect of phospholipid hydrolysis on lipid oxidation in flounder muscle microsomes. Journal of Food Science 46, 12971301.CrossRefGoogle Scholar
Sklan, D, Shelly, M, Makovsky, B, Geyra, A, Klipper, E and Friedman, A 2003. The effect of chronic feeding of diacetoxyscirpenol and T-2 toxin on performance, health, small intestinal physiology and antibody production in turkey poults. British Poultry Science 44, 4652.CrossRefGoogle ScholarPubMed
Solcan, C, Pavel, G, Floristean, V, Chiriac, I, Şlencu, B and Solcan, G 2015. Effect of ochratoxin A on the intestinal mucosa and mucosa-associated lymphoid tissues in broiler chickens. ActaVeterinariaHungarica 63, 3048.Google ScholarPubMed
Stoloff, L 1979. The three eras of fungal toxin research. Journal of the American Oil Chemist’s Society 56, 784.CrossRefGoogle Scholar
Thomas, AB and David, S 1980. Lipid dynamics and lipid-protein interactions in rat enterocyte basolateral and microvillus membranes. Biochemistry 9, 27632769.Google Scholar
Van Hoogevest, P 2017. Review – an update on the use of oral phospholipid excipients. European Journal of Pharmaceutical Sciences 108, 112.CrossRefGoogle ScholarPubMed
Vance, J 2018. Historical perspective: phosphatidylserine and phosphatidylethanolamine from the 1800s to the present. Journal of Lipid Research 59, 923944.CrossRefGoogle ScholarPubMed
Weiss, SB, Smith, SW and Kennedy, EP 1958. The enzymatic formation of lecithin from cytidinediphosphate choline and D-1,2-diglyceride. The Journal of Biological Chemistry 231, 5364.Google Scholar
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