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Effects of methionine hydroxy analogue supplementation on the expression of antioxidant-related genes of acute heat stress-exposed broilers

Published online by Cambridge University Press:  12 October 2017

E. Gasparino ,
A. P. Del Vesco ,
A. S. Khatlab ,
V. Zancanela ,
D. O. Grieser  and
S. C. C. Silva
E. Gasparino*
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
A. P. Del Vesco
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
A. S. Khatlab
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
V. Zancanela
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
D. O. Grieser
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
S. C. C. Silva
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
*
†E-mail: [email protected]
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Abstract

We evaluated the effects of heat stress (HS) and methionine supplementation on biological markers of stress and expression of the genes for superoxide dismutase (SOD), thioredoxin (TRx), thioredoxin reductase 1 (TRxR1) and methionine sulfoxide reductase A (MsrA) in broilers aged 1 to 21 or 22 to 42 days. The broilers were divided into two treatments, one with the recommended level of methionine supplementation (MS, supplementation of dl-2-hydroxy-4-methylthiobutanoic acid (dl-HMTBA)) and one without methionine supplementation (MD). The animals were maintained at a temperature of thermal comfort or one of HS (38°C for 24 h). Mortality was only observed in 42-day-old broilers exposed to HS and fed the MD diet, and the rate was 5%. Starter period: we observed an interaction effect between diet and temperature on the gene expression of TRxR1 and MsrA, and expression of these genes was higher in the HS animals that received the MS diet than that in birds with the MD diet. Grower period: the expression of SOD, TRxR1 and MsrA genes, activities of aspartate aminotransferase (AST) and creatine kinase (CK) and content of creatinine were influenced by both study variables. In the HS animals, the expression of these genes, AST activity and creatinine content increased and CK activity decreased. In the animals on the MD diet, the expression of these genes and AST and creatinine values were higher and the CK activity was lower than those for the birds on the MS diet. Our results indicated that under HS conditions, the supplementation with dl-HMTBA could mitigate major damage caused by stress through the action on some genes related to TRx complex activity.

Keywords

antioxidantbroilerheat stressoxidative stressthioredoxin
Type
Research Article
Information
animal , Volume 12 , Issue 5 , May 2018 , pp. 931 - 939
Copyright
© The Animal Consortium 2017 

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Footnotes

a

Present address: Animal Science Department, Federal University of Sergipe, Aracaju, Sergipe, Brazil.

References

Azad, MAK, Kikusato, M, Sudo, S, Amo, T and Toyomizu, M 2010. Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chicken. Comparative Biochemistry and Physiology Part A 157, 266–271.CrossRefGoogle ScholarPubMed
Birben, E, Sahiner, UM, Sackesen, C, Erzurum, S and Kalayci, O 2012. Oxidative stress and antioxidant defense. World Allergy Organ Journal 5, 9–19.Google Scholar
Del Vesco, AP, Gasparino, E, Grieser, DO, Zancanela, V, Soares, MAM and Neto, ARO 2015a. Effects of methionine supplementation on the expression of oxidative stress-related genes of acute heat stress-exposed broilers. British Journal of Nutrition 113, 549–559.CrossRefGoogle ScholarPubMed
Del Vesco, AP, Gasparino, E, Grieser, DO, Zancanela, V, Voltolini, DN, Khatlab, AS, Guimarães, SEF, Soares, MAM and Neto, ARO 2015b. Effects of methionine supplementation on the expression of protein deposition-related genes in acute heat stress-exposed broilers. PLoS One 10, https://doi.org/10.1371/journal.pone.0115821.CrossRefGoogle Scholar
Fang, YZ, Yang, S and Wu, G 2002. Free radicals, antioxidants, and nutrition. Nutrition 18, 872–879.Google Scholar
Gunst, JJ, Langlois, MR, Delanghe, JR, De Buyzere, ML and Leroux-Roels, GG 1998. Serum creatine kinase activity is not a reliable marker for muscle damage in conditions associated with low extracellular glutathione concentration. Clinical Chemistry 44, 939–943.Google Scholar
Harris, ED 1992. Regulation of antioxidant enzymes. The FASEB Journal 6, 2675–2683.Google Scholar
Khan, HA, Alhomida, AS, Sobki, SH, Habib, SS, Aseri, ZA, Khan, AA and Moghairi, AA 2013. Serum markers of tissue damage and oxidative stress in patients with acute myocardial infarction. Biomedical Research 24, 15–20.Google Scholar
Levine, RL, Laurent, MBS, Berlett, J and Stadtman, ER 1996. Methionine residues as endogenous antioxidants in proteins. Proceedings of the National Academy of Sciences 93, 15036–15040.Google Scholar
Levine, RL, Moskovitz, J and Stadtman, ER 2000. Oxidation of methionine in proteins: roles in antioxidant defense and cellular regulation. International Union of Biochemistry and Molecular Biology Life 50, 301–307.Google Scholar
Luo, S and Levine, RL 2009. Methionine in proteins defends against oxidative stress. Journal Federation of American Societies for Experimental Biology 23, 464–472.CrossRefGoogle ScholarPubMed
Melesse, A, Maak, S, Schmidt, R and Lengerken, GV 2011. Effect of long-term heat stress on key enzyme activities and T3 levels in commercial layer hens. International Journal of Livestock Production 2, 107–116.Google Scholar
Moskovitz, J, Bar-Noy, S, Williams, WM, Requena, J, Berlett, BS and Stadtman, ER 2001. Methionine sulfoxide reductase (MsrA) is a regulator of antioxidant defense and lifespan in mammals. Proceedings of the National Academy of Sciences of the United States of America 98, 12920–12925.Google Scholar
Mujahid, A, Sato, K, Akiba, Y and Toyomizu, M 2006. Acute heat stress stimulates mitochondrial superoxide production in broiler skeletal muscle, possibly via downregulation of uncoupling protein content. Poultry Science 85, 1259–1265.CrossRefGoogle ScholarPubMed
Oliveira, RFM, Donzele, JL, Abreu, MLT, Ferreira, RA, Vaz, RGMV and Cella, PS 2006. Effects of temperature and relative humidity on performance and yield of noble cuts of broilers from 1 to 49 days old. Brazilian Journal of Animal Science 35, 797–803.Google Scholar
Ray, PD, Huang, BW and Tsuji, Y 2012. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular Signalling 24, 981–990.Google Scholar
Rostagno, HS, Albino, LFT, Donzele, JL, Gomes, PC, Oliveira, RF, Lopes, DC, Ferreira, AS and Barreto, SLT 2011. Brazilian tables for birds and pigs: composition of foods and nutritional requirements, 3rd edition. UFV, Viçosa, Brazil.Google Scholar
Shie, H 1997. Oxidative stress: oxidants and antioxidants. Experimental Physiology 82, 291–295.Google Scholar
Song, J, Xiao, K, Ke, YL, Jiao, LF, Hu, CH, Diao, QY, Shi, B and Zou, XT 2014. Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poultry Science 93, 581–588.Google Scholar
Stipanuk, MH 2004. Sulfur amino acid metabolism: pathways for production and removal of homocysteine and cysteine. Annual Review of Nutrition 24, 539–577.CrossRefGoogle ScholarPubMed
Swennen, Q, Geraert, AP, Mercier, Y, Everaert, N, Stinckens, A, Willemsen, H, Li, Y, Decuypere, E and Buyse, J 2011. Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or DL-methionine supplementation on performance and oxidative status of broiler chickens. British Journal of Nutrition 106, 1845–1854.Google Scholar
Weissbach, H, Resnick, L and Brot, N 2005. Methionine sulfoxide reductases: history and cellular role in protecting against oxidative damage. Biochimica et Biophysica Acta 1703, 203–212.Google Scholar
Willemsen, H, Swennen, Q, Everaert, N, Geraert, PA, Mercier, Y, Stinckens, A, Decuypere, E and Buyse, J 2011. Effects of dietary supplementation of methionine and its hydroxy analog DL-2-hydroxy-4-methylthiobutanoic acid on growth performance, plasma hormone levels, and the redox status of broiler chickens expose to high temperatures. Poultry Science 90, 2311–2320.Google Scholar
Yang, L, Tan, GY, Fu, QF, Feng, JH and Zhang, MH 2010. Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration, ROS production and lipid peroxidation in broiler chickens. Comparative Biochemistry Physiology Part C. Toxicology & Pharmacology 151, 204–208.Google Scholar