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Effects of dose and route of administration of genistein on isoflavone concentrations in post-weaned and gestating sows

Published online by Cambridge University Press:  01 February 2013

C. Farmer ,
P. Robertson  and
G. S. Gilani
C. Farmer*
Affiliation:
Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, 2000 College Street, Sherbrooke, QC J1M 0C8 Canada
P. Robertson
Affiliation:
Nutrition Research Division, Health Canada, Ottawa, ON K1A 0K9 Canada
G. S. Gilani
Affiliation:
Nutrition Research Division, Health Canada, Ottawa, ON K1A 0K9 Canada
*
†E-mail: [email protected]
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Abstract

Phytoestrogens could be a useful tool in swine husbandry practices because of their structural and functional similarities to estradiol. The goal of this study was to compare various routes and doses of administration of the phytoestrogen genistein in sows of two different physiological statuses. Circulating concentrations of isoflavones, estradiol and IGF-I were determined. In experiment 1, 65 sows were equally divided into the five following groups, between days 3 and 5 of the first or second estrous cycle post weaning: (1) controls (CTL); (2) 1 g of genistein fed daily (OR1); (3) 2 g of genistein fed daily (OR2); (4) two daily i.m. injections of 200 mg of genistein (IM400); and (5) two daily i.m. injections of 400 mg of genistein (IM800). Treatments were carried out for 10 days. In experiment 2, 10 sows were equally divided into two groups on day 90 of gestation, namely, controls (CTL) or 2 g of genistein fed daily for 10 days (OR2). In both trials, jugular blood samples were collected on days 1 (before treatment), 5 and 10 at 0730 h. In experiment 1, a blood sample was also collected at 1730 h on day 10 for CTL, IM400 and IM800 sows. In experiment 1, circulating concentrations of genistein on days 5 and 10 were greater in OR2, IM400 and IM800 than in CTL and OR1 group sows (P < 0.01). Daily dietary supplementation with 2 g of genistein resulted in blood concentrations that were similar to those in animals given daily two i.m. injections of 200 mg. Values of all isoflavones, except equol, which was not detectable, were greater in PM than in AM on day 10 (P < 0.01). In experiment 2, genistein concentrations were greater in OR2 compared with CTL on days 5 and 10 (P ⩽ 0.05). There was no difference in the genistein response to OR2 because of physiological status (i.e. weaned v. gestating, P > 0.1). Estradiol and IGF-I concentrations were not altered by any of the treatments (P > 0.1). Providing genistein either per os or via i.m. injections increased circulating concentrations of genistein in female swine within 5 days of the onset of treatment. The genistein response to i.m. injections of genistein was similar in weaned and late-pregnant sows, even though endogenous concentrations of estradiol differed. This response was specific in that estradiol, IGF-I and isoflavones other than genistein were not affected by treatments.

Keywords

dose–responsegenisteinisoflavonephytoestrogenpigssows
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 6 , June 2013 , pp. 983 - 989
Copyright
Copyright © Her Majesty the Queen in Right of Canada, represented by the Minister of Agriculture and Agri-Food Canada and the Minister of Health Canada 2013

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References

Agriculture and Agri-Food Canada 1993. Recommended code of practice for the care and handling of farm animals – pigs. Publication No. 1898E. Agriculture and Agri-Food Canada, Ottawa, ON.Google Scholar
Chen, WF, Gao, QG, Wong, MS 2007. Mechanism involved in genistein activation of insulin-like growth factor 1 receptor expression in human breast cancer cells. British Journal of Nutrition 98, 1120–1125.Google Scholar
Cimafranca, MA, Davila, J, Ekman, GC, Andrews, RN, Neese, SL, Peretz, J, Woodling, KA, Helferich, WG, Sarkar, J, Flaws, JA, Schantz, SL, Doerge, DR, Cooke, PS 2010. Acute and chronic effects of oral genistein administration in neonatal mice. Biology of Reproduction 83, 114–121.Google Scholar
Clapper, J, Tomlin, A 2012. Effects of the phytoestrogen genistein on the porcine anterior pituitary insulin-like growth factor system. Domestic Animal Endocrinology 42, 173–182.Google Scholar
Dinsdale, EC, Ward, WE 2010. Early exposure to soy isoflavones and effects on reproductive health: a review of human and animal studies. Nutrients 2, 1156–1187.Google Scholar
Farmer, C, Palin, MF, Gilani, GS, Weiler, H, Vignola, M, Choudhary, RK, Capuco, AV 2010. Dietary genistein stimulates mammary hyperplasia in gilts. Animal 4, 454–465.Google Scholar
Ford, JA Jr 2003. Estrogenic effects of genistein in ovariectomized gilts. PhD, University of Illinois, Urbana-Champaign, IL, USA.Google Scholar
Ford, JA Jr, Clark, SG, Walters, EM, Wheeler, MB, Hurley, WL 2006. Estrogenic effects of genistein on reproductive tissues of ovariectomized gilts. Journal of Animal Science 84, 834–842.CrossRefGoogle ScholarPubMed
Gardner, CD, Chatterjee, LM, Franke, AA 2009. Effects of isoflavone supplements vs. soy foods on blood concentrations of genistein and daidzein in adults. Journal of Nutritional Biochemistry 20, 227–234.Google Scholar
Gu, L, House, SE, Prior, RL, Fang, N, Ronis, MJJ, Clarkson, TB, Wilson, ME, Badger, TM 2006. Metabolic phenotypes of isoflavones differ among female rats, pigs, monkeys, and women. Journal of Nutrition 136, 1215–1221.Google Scholar
Jefferson, WN, Williams, CJ 2011. Circulating levels of genistein in the neonate, apart from dose and route, predict future adverse female reproductive outcomes. Reproductive Toxicology 31, 272–279.Google Scholar
Jefferson, WN, Padilla-Banks, E, Newbold, RR 2007. Disruption of the female reproductive system by the phytoestrogen genistein. Reproductive Toxicology 23, 308–316.Google Scholar
Kuhn, G, Hennig, U, Kalbe, C, Rehfeldt, C, Ren, MQ, Moors, S, Degen, GH 2004. Growth performance, carcass characteristics and bioavailability of isoflavones in pigs fed soy bean based diets. Archives of Animal Nutrition 58, 265–276.Google Scholar
Li, FN, Li, LL, Yang, HS, Yuan, XX, Zhang, B, Geng, MM, Xiao, CW, Yin, YL 2011. Regulation of soy isoflavones on weight gain and fat percentage: evaluation in a Chinese Guangxi minipig model. Animal 5, 1903–1908.Google Scholar
Lister, CE, Skinner, MA, Hunter, DC 2007. Fruits, vegetables and their phytochemicals for bone and joint health. Current Topics in Nutraceutical Research 5, 67–82.Google Scholar
Nilsson, S, Mäkelä, S, Treuter, E, Tujague, M, Thomsen, J, Andersson, G, Enmark, E, Pettersson, K, Warner, M, Gustafsson, JA 2001. Mechanisms of estrogen action. Physiological Reviews 81, 1535–1565.CrossRefGoogle ScholarPubMed
Norrby, M, Madsen, MT, Saravia, F, Lundeheim, N, Madej, A 2011. Genistein alters the release of oxytocin, prostaglandins, cortisol and LH during insemination in gilts. Reproduction in Domestic Animals 46, 316–324.Google Scholar
Plante, PA, Laforest, JP, Farmer, C 2011. Effect of supplementing the diet of lactating sows with NuPro® on sow lactation performance and piglet growth. Canadian Journal of Animal Science 91, 295–300.Google Scholar
Rehfeldt, C, Kalbe, C, Nürnberg, G, Mau, M 2009. Dose-dependent effects of genistein and daidzein on protein metabolism in porcine myotube cultures. Journal of Agricultural Food Chemistry 57, 852–857.Google Scholar
SAS 1998. SAS/STAT user's guide (version 6), 4th edition. SAS Institute Inc., Cary, NC, USA.Google Scholar
Sepehr, E, Coke, G, Robertson, P, Gilani, GS 2007. Bioavailability of soy isoflavones in rats. Part I: application of accurate methodology for studying the effects of gender and source of isoflavones. Molecular Nutrition and Food Research 51, 799–812.Google Scholar
Sepehr, E, Robertson, P, Gilani, GS, Cooke, G, Lau, BP-Y 2006. An accurate and reproducible method for the quantitative analysis of isoflavones and their metabolites in rat plasma using liquid chromatography/mass spectrometry combined with photodiode array detection. Journal of Association of Analytical Chemists International 89, 1158–1167.Google Scholar
Sun Hwang, C, Seok Kwak, H, Jae Lim, H, Hee Lee, S, Soon Kang, Y, Boo Choe, T, Gil Hur, H, Ok Han, K 2006. Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration. Journal of Steroid Biochemistry and Molecular Biology 101, 246–253.Google Scholar
Warri, A, Saarinen, NM, Makela, S, Hilakivi-Clarke, L 2008. The role of early life genistein exposures in modifying breast cancer risk. British Journal of Cancer 98, 1485–1493.Google Scholar