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Effects of combined liver and udder biopsying on the acute phase response of dairy cows with experimentally induced E. coli mastitis

Published online by Cambridge University Press:  18 July 2013

M. Khatun ,
P. Sørensen ,
K. L. Ingvartsen ,
M. Bjerring  and
C. M. Røntved
M. Khatun
Affiliation:
Department of Animal Science, Aarhus University, PO Box 50, DK-8300 Tjele, Denmark Center for Quantitative Genetics, Department of Molecular Biology and Genetics, Aarhus University, PO Box 50, DK-8300 Tjele, Denmark Momena Khatun, Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
P. Sørensen
Affiliation:
Center for Quantitative Genetics, Department of Molecular Biology and Genetics, Aarhus University, PO Box 50, DK-8300 Tjele, Denmark
K. L. Ingvartsen
Affiliation:
Department of Animal Science, Aarhus University, PO Box 50, DK-8300 Tjele, Denmark
M. Bjerring
Affiliation:
Department of Animal Science, Aarhus University, PO Box 50, DK-8300 Tjele, Denmark
C. M. Røntved*
Affiliation:
Department of Animal Science, Aarhus University, PO Box 50, DK-8300 Tjele, Denmark
*
E-mail: [email protected]
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Abstract

A minimally invasive biopsy technique was evaluated for udder tissue collection in dairy cows with Escherichia coli mastitis. Meanwhile, the effect of taking repeated liver and udder biopsies on the systemic and local acute phase response (APR) of the dairy cows was investigated during the disease. The cows were divided into a biopsy group (B) (n = 16) and a no-biopsy group (NB) (n = 16) and were sampled in the acute disease stage and in the recovery stage. The cows’ pre-disease period served as a control period for establishing baseline values for the investigated parameters. A total of 32 Holstein-Friesian cows were inoculated with 20 to 40 colony-forming units (cfu) of E. coli in one front quarter at 0 hour. Liver biopsies were collected at −144, 12, 24 and 192 h, and udder biopsies were collected at 24 and 192 h post E. coli inoculation (PI) using a minimally invasive biopsy technique. Effects of combined biopsying were investigated by recording production traits, clinical response, and measuring inflammatory milk and blood parameters: E. coli, somatic cell count, milk amyloid A (MAA) levels, white blood cell count, polymorphonuclear neutrophilic leukocyte numbers and serum amyloid A levels at several time points. E. coli inoculation changed all production parameters and the clinical and inflammatory response in all cows except one that was not infected. Combined biopsying had no constant or transient effect on the daily feed intake, the clinical responsiveness or the blood parameters, but affected the daily milk yield and some milk parameters transiently, that is, the presence of blood in milk, increased E. coli counts and MAA levels during the acute disease stage. Combined biopsying had no effect on the parameters in the recovery stage apart from the presence of blood in the milk. In conclusion, although, a minimally invasive biopsy technique was used, tissue damages could not be avoided when biopsying and they transiently affected the inflammatory parameters in the mammary gland. Nevertheless, we believe combined biopsying of liver and udder is as an acceptable approach to study the systemic and local APR in dairy cows during E. coli mastitis, if the timing of biopsying and other types of sampling is planned accordingly.

Keywords

biopsycattleE. coli mastitisheparmammary gland
Type
Behaviour, welfare and health
Information
animal , Volume 7 , Issue 10 , October 2013 , pp. 1721 - 1730
Copyright
Copyright © The Animal Consortium 2013 

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References

Bannerman, D, Paape, M, Lee, JW, Zhao, X, Hope, J, Rainard, P 2004. Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection. Clinical and Diagnostic Laboratory Immunology 11, 463–472.Google ScholarPubMed
Blum, JW, Dosogne, H, Hoeben, D, Vangroenweghe, F, Hammon, HM, Bruckmaier, RM, Burvenich, C 2000. Tumor necrosis factor-alpha and nitrite/nitrate responses during acute mastitis induced by Escherichia coli infection and endotoxin in dairy cows. Domestic Animal Endocrinology 19, 223–235.Google Scholar
Bradley, AJ, Leach, KA, Breen, JE, Green, LE, Green, MJ 2007. Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales. Veterinary Record 160, 253–257.Google Scholar
Buitenhuis, B, Røntved, CM, Edwards, SM, Ingvartsen, KL, Sørensen, P 2011. In depth analysis of genes and pathways of the mammary gland involved in the pathogenesis of bovine Escherichia coli-mastitis. BMC Genomics 12, 130.Google Scholar
Burvenich, C, Van Merris, V, Mehrzad, J, Diez-Fraile, A, Duchateau, L 2003. Severity of E. coli mastitis is mainly determined by cow factors. Veterinary Research 34, 521–564.CrossRefGoogle ScholarPubMed
Cray, C, Zaias, J, Altman, NH 2009. Acute phase response in animals: a review. Comparative Medicine 59, 517–526.Google ScholarPubMed
Farr, VC, Stelwagen, K, Cate, LR, Molenaar, AJ, Mcfadden, TB, Davis, SR 1996. An improved method for the routine biopsy of bovine mammary tissue. Journal of Dairy Science 79, 543–549.CrossRefGoogle ScholarPubMed
Fogsgaard, KK, Røntved, CM, Sørensen, P, Herskin, MS 2012. Sickness behavior in dairy cows during Eschericia coli mastitis. Journal of Dairy Science 95, 630–638.Google Scholar
Jacobsen, S, Niewold, TA, Kornalijnslijper, E, Toussaint, MJM, Gruys, E 2005. Kinetics of local and systemic isoforms of serum amyloid A in bovine mastitic milk. Veterinary Immunology and Immunopathology 104, 21–31.CrossRefGoogle ScholarPubMed
Hertl, JA, Schukken, YH, Bar, D, Bennett, GJ, Gonzalez, RN, Rauch, BJ, Welcome, FL, Tauer, LW, Grohn, YT 2011. The effect of recurrent episodes of clinical mastitis caused by Gram-positive and Gram-negative bacteria and other organisms on mortality and culling in Holstein dairy cows. Journal of Dairy Science 94, 4863–4877.Google Scholar
Jørgensen, HB, Buitenhuis, B, Røntved, CM, Jiang, L, Ingvartsen, KL, Sørensen, P 2012. Transcriptional profiling of the bovine hepatic response to experimentally induced E. coli mastitis. Physiological Genomics 44, 595–606.CrossRefGoogle ScholarPubMed
Khatun, M, Sørensen, P, Jørgensen, HB, Sahana, G, Sørensen, LP, Lund, MS, Ingvartsen, KL, Buitenhuis, AJ, Vilkki, J, Bjerring, M, Thomasen, JR, Røntved, CM 2013. Effects of Bos taurus autosome 9-located quantitative trait loci haplotypes on the disease phenotypes of dairy cows with experimentally induced Escherichia coli mastitis. Journal of Dairy Science 96, 1820–1833.Google Scholar
Knight, CH, Hillerton, JE, Teverson, RM, Winter, A 1992. Biopsy of the bovine mammary gland. British Veterinary Journal 148, 129–132.Google Scholar
Kristensen, T 1986. Method for estimation of body condition in dairy cows. Report No. DK8621430, Statens Husdyrbrugsforsoeg, Copenhagen, Denmark, 59–76pp.Google Scholar
Mitterhuemer, S, Petzl, W, Krebs, S, Mehne, D, Klanner, A, Wolf, E, Zerbe, H, Blum, H 2010. Escherichia coli infection induces distinct local and systemic transcriptome responses in the mammary gland. BMC Genomics 11, 138.Google Scholar
Mølgaard, L, Damgaard, BM, Bjerre-Harpøth, V, Herskin, MS 2012. Effects of percutaneous needle liver biopsy on dairy cow behaviour. Research of Veterinary Science 93, 1248–1254.Google Scholar
Oxender, WD, Askew, EW, Benson, JD, Emery, RS 1971. Biopsy of liver, adipose tissue and mammary gland of lactating cows. Journal of Dairy Science 54, 286–288.Google Scholar
Paape, M, Mehrzad, J, Zhao, X, Detilleux, J, Burvenich, C 2002. Defense of the bovine mammary gland by polymorphonuclear neutrophil leukocytes. Journal of Mammary Gland Biology and Neoplasia 7, 109–121.Google Scholar
Petrovski, KR, Trajcev, M, Buneski, G 2006. A review of the factors affecting the costs of bovine mastitis. Journal of South African Veterinary Association 77, 52–60.CrossRefGoogle ScholarPubMed
Pezeshki, A, Stordeur, P, Wallemacq, H, Schynts, F, Stevens, M, Boutet, P, Peelman, LJ, Spiegeleer, BD, Duchateau, L, Bureau, F, Burvenich, C 2011. Variation of inflammatory dynamics and mediators in primiparous cows after intramamamry challenge with Escherichia coli. Veterinary Research 42, 15.Google Scholar
Rasmussen, DB, Fogsgaard, K, Rontved, CM, Klaas, IC, Herskin, MS 2011. Changes in thermal nociceptive responses in dairy cows following experimentally induced Escherichia coli mastitis. Acta Veterinaria Scandinavica 53, 32.CrossRefGoogle ScholarPubMed
Sheehy, PA, Della-Vedova, JJ, Nicholas, KR, Wynn, PC 2004. Hormone-dependent milk protein gene expression in bovine mammary explants from biopsies at different stages of pregnancy. Journal of Dairy Research 71, 135–140.Google Scholar
Suojala, L, Orro, T, Jarvinen, H, Saatsi, J, Pyörälä, S 2008. Acute phase response in two consecutive experimentally induced E. coli intramammary infections in dairy cows. Acta Veterinaria Scandinavica 50, 18.Google Scholar
Vels, L, Røntved, CM, Bjerring, M, Ingvartsen, KL 2009. Cytokine and acute phase protein gene expression in repeated liver biopsies of dairy cows with a lipopolysaccharide-induced mastitis. Journal of Dairy Science 92, 922–934.CrossRefGoogle ScholarPubMed