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Effects of an immunomodulatory feed additive on the development of mastitis in a mouse infection model using four bovine-origin isolates

Published online by Cambridge University Press:  22 September 2010

A. D. Rowson ,
Y.-Q. Wang ,
E. Aalseth ,
N. E. Forsberg  and
S. B. Puntenney
A. D. Rowson
Affiliation:
OmniGen Research, LLC. 1767 NW Kings Blvd. Corvallis, OR 97330, USA
Y.-Q. Wang
Affiliation:
OmniGen Research, LLC. 1767 NW Kings Blvd. Corvallis, OR 97330, USA
E. Aalseth
Affiliation:
Dairy Consulting 6514 113th Avenue NE, Lake Stevens, WA 98258, USA
N. E. Forsberg*
Affiliation:
OmniGen Research, LLC. 1767 NW Kings Blvd. Corvallis, OR 97330, USA
S. B. Puntenney
Affiliation:
OmniGen Research, LLC. 1767 NW Kings Blvd. Corvallis, OR 97330, USA
*
†E-mail: [email protected]
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Abstract

The goal of this study was to examine the ability of a commercially available feed additive (OmniGen-AF) to reduce mammary infections caused by a single strain of mastitic pathogens (Streptococcus uberis, Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae) and to examine the effects of the additive on markers of mammary immunity. Four experiments were completed using a murine model of bovine mastitis. Infection progression was examined using Sybr-green- and TaqMan-based quantitative PCR assays of 16S ribosomal DNA. Infection of the mammary gland with all pathogens caused rapid (24 to 48 h) appearance of pathogen DNA in mammary tissue. Provision of the feed additive for 2 weeks before infection significantly (P < 0.05) reduced the extent of pathogen DNA accumulation in models of S. uberis, E. coli and S. aureus infection. The additive was ineffective in reducing mammary infections caused by K. pneumoniae. We examined mechanisms of action of the additive through assessment of mammary concentrations of mammary myeloperoxidase (MPO), major histocompatibility complex 2 class II (MHC) and macrophage inflammatory protein-1α (MIP) messenger RNA (mRNA) concentrations and by examining serum complement C3 concentration. Infection of the mammary gland increased concentrations of MPO and MHC mRNAs (P < 0.05). Ability of the pathogen to elicit changes in mammary MPO and MHC gene expression was enhanced by the provision of the additive for 2 weeks before infection. These data imply that the additive increased the mammary inflammatory response and increased antigen presentation during a mammary infection. Value of the additive in preventing mastitis in cattle awaits additional studies using a bovine model and further evaluation of additional strains of the pathogens used in this study.

Keywords

mastitisimmunitymurineOmniGen-AF
Type
Full Paper
Information
animal , Volume 5 , Issue 2 , February 2011 , pp. 220 - 229
Copyright
Copyright © The Animal Consortium 2010

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References

Barkema, HW, Schukken, YH, Lam, JGM, Beiboer, ML, Wilmink, H, Benedictus, G, Brand, A 1998. Incidence of clinical mastitis in dairy herds grouped in three categories by bulk milk somatic cell counts. Journal of Dairy Science 81, 411–419.CrossRefGoogle ScholarPubMed
Boulanger, D, Brouillette, E, Jasper, F, Malouin, F, Mainil, J, Bureau, F, Lekeux, P 2007. Helenalin reduces Staphylococcus aureus infection in vitro and in vivo. Veterinary Microbiology 119, 330–338.CrossRefGoogle ScholarPubMed
Bradley, AJ 2002. Bovine mastitis: an evolving disease. Veterinary Journal 164, 116–128.CrossRefGoogle ScholarPubMed
Brouillette, E, Grondin, G, Lefebvre, C, Talbot, G, Malouin, F 2004. Mouse mastitis model of infection for antimicrobial compound efficacy studies against intracellular and extracellular forms of Staphylococcus aureus. Veterinary Microbiology 101, 253–262.CrossRefGoogle ScholarPubMed
Cebra, CK, Garry, FB, Dinsmore, RP 1996. Naturally occurring acute coliform mastitis in Holstein cattle. Journal of Veterinary Internal Medicine 10, 252–257.CrossRefGoogle ScholarPubMed
Chandler, RL 1969. Preliminary report on the production of experimental mastitis in the mouse. Veterinary Record 84, 671–672.CrossRefGoogle ScholarPubMed
Chandler, RL 1970. Experimental bacterial mastitis in the mouse. Journal of Medical Microbiology 3, 273–282.CrossRefGoogle ScholarPubMed
del Rio, B, Martin, MC, Martinez, N, Magadan, AH, Alvarez, MA 2008. Multiplex fast real-time PCR for quantitative detection and identification of cos- and pac-type Streptococcus thermophilus bacteriophages. Applied and Environmental Microbiology 74, 4779–4781.CrossRefGoogle ScholarPubMed
Faith, M, Sukumaran, A, Pulimood, AB, Jacob, M 2008. How reliable an indicator of inflammation is myeloperoxidase activity? Clinica Chimica Acta 396, 23–25.CrossRefGoogle Scholar
Gomez, MI, Sordelli, DO, Buzzola, FR, Garcia, VE 2002. Induction of cell-mediated immunity to Staphylococcus aureus in the mouse mammary gland by local immunization with a live attenuated mutant. Infection and Immunity 70, 4254–4260.CrossRefGoogle ScholarPubMed
Gruet, P, Maincent, P, Berthelot, X, Kaltosatos, V 2001. Bovine mastitis and intramammary drug delivery: review and perspectives. Advanced Drug Delivery Reviews 50, 245–259.CrossRefGoogle ScholarPubMed
Lee, J-W, Paape, MJ, Zhao, X 2003. Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice. Veterinary Research 34, 307–316.CrossRefGoogle ScholarPubMed
Li, W, Drake, MA 2001. Development of a quantitative competitive PCR assay for detection and quantification of Escherichia coli O157:H7 cells. Applied and Environmental Microbiology 67, 3291–3294.CrossRefGoogle ScholarPubMed
Livak, KJ, Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402–408.CrossRefGoogle Scholar
Mamo, W, Froman, G, Muller, HP 2000. Protection induced in mice vaccinated with recombinant collagen-binding protein (CnBP) and alpha-toxoid against intramammary infection with Staphylococcus aureus. Microbiology and Immunology 44, 381–384.CrossRefGoogle ScholarPubMed
Murphy, K, Travers, P, Walport, M 2008. Janeway’s Immunobiology. Garland Science, New York, NY.Google Scholar
National Animal Health Monitoring System (NAHMS) 2007. Dairy 2007. Dairy Part 1: Reference of dairy cattle health and management practices in the United States, 2007. USDA:APHIS:VS:CEAH, Fort Collins, CO.Google Scholar
Pan, YJ, Fang, HC, Yang, HC, Lin, TL, Hsieh, PF, Tsai, FC, Keynan, Y, Wang, JT 2008. Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype. Journal of Clinical Microbiology 46, 2231–2240.CrossRefGoogle Scholar
Shannon, KE, Lee, DY, Trevors, JT, Beaudette, LA 2007. Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment. Science of the Total Environment 382, 121–129.CrossRefGoogle ScholarPubMed
Sordillo, LM, Nickerson, SC 1986. Morphological changes caused by experimental Streptococcus uberis mastitis in mice following intramammary infusion of pokeweed mitogen. Proceedings of the Society for Experimental Biology and Medicine 182, 522–530.CrossRefGoogle ScholarPubMed
Straus, DC 1987. Production of an extracellular toxic complex by various strains of Klebsiella pneumoniae. Infection and Immunity 55, 44–48.CrossRefGoogle ScholarPubMed
Studer, E, Schaeren, W, Naskova, J, Pfaeffli, H, Kaufmann, T, Kirchhofer, M, Steiner, A, Graber, HU 2008. A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Journal of Dairy Science 91, 1893–1902.CrossRefGoogle ScholarPubMed
Wada, K, Endo, H, Takahashi, J, Yano, H, Watanabe, E, Koya, M, Abe, S, Katoh, T, Ogata, Y 2008. Influence of Aspergillus fumigatus and effect of the yeast cell wall-mixed additive (OmniGen-AF) on dairy herd. Journal of the Japanese Society of Clinical Infectious Diseases 3, 13–16.Google Scholar
Walia, SK, Madhavan, T, Chugh, TD, Sharma, KB 1987. Characterization of self-transmissible plasmids determining lactose fermentation and multiple antibiotic resistance in clinical strains of Klebsiella pneumoniae. Plasmid 17, 3–12.CrossRefGoogle ScholarPubMed
Wang, Y-Q, Puntenney, SB, Burton, JL, Forsberg, NE 2007. Ability of a commercial feed additive to modulate the expression of innate immunity in sheep immunosuppressed with dexamethasone. Animal 1, 945–951.CrossRefGoogle ScholarPubMed
Wang, Y-Q, Puntenney, SB, Burton, JL, Forsberg, NE 2009. Use of gene profiling to evaluate the effects of a feed additive on immune function in periparturient dairy cattle. Journal of Animal Physiology and Animal Nutrition 93, 66–75.CrossRefGoogle ScholarPubMed