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Effect of cholesterol-loaded cyclodextrins on bull and goat sperm processed with fast or slow cryopreservation protocols

Published online by Cambridge University Press:  21 February 2014

E. Mocé ,
C. Tomás ,
E. Blanch  and
J. K. Graham
E. Mocé*
Affiliation:
Centro de Tecnología Animal – Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono “La Esperanza”, 100. Apdo. 187. 12400-Segorbe (Castellón), Spain
C. Tomás
Affiliation:
Centro de Tecnología Animal – Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono “La Esperanza”, 100. Apdo. 187. 12400-Segorbe (Castellón), Spain
E. Blanch
Affiliation:
Centro de Tecnología Animal – Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono “La Esperanza”, 100. Apdo. 187. 12400-Segorbe (Castellón), Spain
J. K. Graham
Affiliation:
Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
*
† E-mail: [email protected]
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Abstract

Cholesterol-loaded cyclodextrins (CLC) added to the sperm before cryopreservation enhance sperm quality after freeze-thawing in several cold shock-sensitive species, including cattle and goats. However, all studies conducted to date have used conventional protocols, in which sperm are cooled slowly to 5°C before freezing. As cholesterol plays a significant role in sperm cold shock resistance, it is possible that CLC-treated sperm can withstand cooling damage when the sperm are not cooled slowly to 5°C before freezing. In this study, we determined whether CLC-treated goat (1 mg CLC/120×106 sperm) and bull (2 mg CLC/120×106 sperm) sperm quality, after thawing, was different for sperm frozen using conventional protocols (including a slow cooling phase to 5ºC) and protocols in which the sperm were frozen from room temperature, without cooling the sperm slowly to 5°C before freezing. CLC-treated sperm exhibited higher percentages of plasma membrane-intact sperm than control sperm when cryopreserved using conventional protocols. In addition, CLC treatment enhanced both sperm motility and plasma membrane integrity when sperm were frozen directly from room temperature. However, this treatment did not fully prevent the damage of the sperm after cooling rapidly and subsequent freezing, as the sperm quality was lower than that presented by the samples frozen using the conventional protocol. The results are promising, but studies to optimize the protocols for freezing sperm directly from room temperature need to be conducted, as well as studies to determine how cryopreserving sperm in this manner affects other sperm functions.

Keywords

cooling ratesemencattlebuck
Type
Full Paper
Information
animal , Volume 8 , Issue 5 , May 2014 , pp. 771 - 776
Copyright
© The Animal Consortium 2014 

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References

Amann, RP and Pickett, BW 1987. Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. Equine Veterinary Science 7, 145–173.CrossRefGoogle Scholar
Drobnis, EZ, Crowe, LM, Berger, T, Anchordoguy, TJ, Overstreet, JW and Crowe, JH 1993. Cold shock damage is due to lipid phase transitions in cell membranes: a demonstration using sperm as a model. Journal of Experimental Zoology 265, 432–437.Google Scholar
Konyali, C, Tomás, C, Blanch, E, Gómez, EA, Graham, JK and Mocé, E 2013. Optimizing conditions for treating goat semen with cholesterol-loaded cyclodextrins prior to freezing to improve cryosurvival. Cryobiology 67, 124–131.Google Scholar
Ladbrooke, BD, Williams, RM and Chapman, D 1968. Studies on lecithin-cholesterol-water interactions by differential calorimetry and X-ray diffraction. Biochimica et Biophysica Acta 150, 333–340.Google Scholar
Leboeuf, B, Restall, B and Salamon, S 2000. Production and storage of goat semen for artificial insemination. Animal Reproduction Science 62, 113–141.Google Scholar
Mocé, E and Graham, JK 2006. Cholesterol-loaded cyclodextrins added to fresh bull ejaculates improve sperm cryosurvival. Journal of Animal Science 84, 826–833.Google Scholar
Mocé, E, Blanch, E, Tomás, C and Graham, JK 2010. Use of cholesterol in sperm cryopreservation: present moment and perspectives to future. Reproduction in Domestic Animals 45, 57–66.Google Scholar
Ohvo-Rekilä, H, Ramstedt, B, Lappimäki, P and Slotte, JP 2002. Cholesterol interactions with phospholipids in membranes. Progress in Lipid Research 41, 66–97.Google Scholar
Purdy, PH and Graham, JK 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology 48, 36–45.CrossRefGoogle Scholar
Rana, APS, Majumder, GC, Misra, S and Ghosh, A 1991. Lipid changes of goat sperm plasma membrane during epididymal maturation. Biochimica et Biophysica Acta 1061, 185–196.CrossRefGoogle ScholarPubMed
Rottem, S, Yashouv, J, Ne’eman, A and Razin, A 1973. Lipid composition, ultrastructure and biological properties of membranes from Mycoplasma mycoides var. capri cells adapted to grow with low cholesterol concentrations. Biochimica et Biophysica Acta 323, 495–508.Google Scholar
Silvestre, MA, Salvador, I, Sanchez, JP and Gόmez, EA 2004. Effect of changing female stimulus on intensive semen collection in young Murciano-Granadina male goats. Journal of Animal Science 82, 1641–1645.Google Scholar
Watson, PF 1981. The effects of cold shock on sperm cell membranes. In Effects of low temperatures on biological membranes (eds GJ Morris and A Clark), pp. 189–218. Academic Press, London, UK.Google Scholar