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The effect of chitooligosaccharide supplementation on intestinal morphology, selected microbial populations, volatile fatty acid concentrations and immune gene expression in the weaned pig

Published online by Cambridge University Press:  02 March 2012

A. M. Walsh ,
T. Sweeney ,
B. Bahar ,
B. Flynn  and
J. V. O'Doherty
A. M. Walsh
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
T. Sweeney
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
B. Bahar
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
B. Flynn
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
J. V. O'Doherty*
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
*
†E-mail: [email protected]
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Abstract

An experiment (complete randomised design) was conducted to investigate the effects of supplementing different molecular weights (MW) of chitooligosaccharide (COS) on intestinal morphology, selected microbial populations, volatile fatty acid (VFA) concentrations and the immune status of the weaned pig. A total of 28 piglets (24 days of age, 9.1 kg (± s.d. 0.80) live weight) were assigned to one of four dietary treatments for 8 days and then sacrificed. The treatments were (1) control diet (0 ppm COS), (2) control diet plus 5 to 10 kDa COS, (3) control diet plus 10 to 50 kDa COS and (4) control diet plus 50 to 100 kDa COS. The COS was included in dietary treatments at a rate of 250 mg/kg. Tissue samples were taken from the duodenum, jejunum and ileum for morphological measurements. Digesta samples were taken from the proximal colon to measure lactobacilli and Escherichia coli populations and digesta samples were taken from the caecum and proximal colon for VFA analysis. Gene expression levels for specific cytokines were investigated in colonic tissue of the pig. Supplementation of different MW of COS had no significant effect on pig performance during the post-weaning period (days 0 to 8; P > 0.05). The inclusion of COS at all MW in the diet significantly reduced faecal scores compared with the control treatment (P < 0.01). Pigs fed the 10 to 50 kDa COS had a higher villous height (P < 0.05) and villous height : crypt depth ratio (P < 0.05) in the duodenum and the jejunum compared with the control treatment. Pigs fed the 5 to 10 kDa COS had a lower lactobacilli population (P < 0.05) and E. coli population (P < 0.05) in the colon compared with the control group. Pigs offered the 5 to 10 kDa COS had significantly lower levels of acetic acid and valeric acid compared with the control group (P < 0.05). The inclusion of different MW of COS had no significant effect on the expression of the cytokines tumour necrosis factor-α, Interleukin (IL)-6, IL-8 and IL-10 in the gastro-intestinal tract of the weaned pig. The current results indicate that a lower MW of 5 to 10 kDa COS possessed an antibacterial activity, while the higher MW of 10 to 50 kDa was optimum for enhancing the intestinal structure.

Keywords

chitooligosaccharidepigmicrobiologyintestinal morphology
Type
Full Paper
Information
animal , Volume 6 , Issue 10 , October 2012 , pp. 1620 - 1626
Copyright
Copyright © The Animal Consortium 2012

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References

Chomczynski, P, Sacchi, N 2006. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nature Protocols 1, 581–585.CrossRefGoogle Scholar
Chung, Y-C, Chen, C-Y 2008. Antibacterial characteristics and activity of acid-soluble chitosan. Journal of Bioresource Technology 99, 2806–2814.CrossRefGoogle ScholarPubMed
Fairbrother, JM, Nadeau, É, Gyles, CL 2005. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Animal Health Research Reviews 6, 17–39.CrossRefGoogle ScholarPubMed
Htoo, JK, Araiza, BA, Sauer, WC, Rademacher, M, Zhang, Y, Cervantes, M, Zijlstra, RT 2007. Effect of dietary protein content on ileal amino acid digestibility, growth performance, and formation of microbial metabolites in ileal and cecal digesta of early-weaned pigs. Journal of Animal Science 85, 3303–3312.CrossRefGoogle ScholarPubMed
Jeon, Y-J, Park, P-J, Kim, S-K 2001. Antimicrobial effect of chitooligosaccharides produced by bioreactor. Journal of Carbohydrate Polymers 44, 71–76.CrossRefGoogle Scholar
Kim, S-K, Rajapakse, N 2005. Enzymatic production and biological activities of chitosan oligosaccharides (COS): a review. Journal of Carbohydrate Polymers 62, 357–368.CrossRefGoogle Scholar
Kittur, FS, Vishu Kumar, AB, Varadaraj, MC, Tharanathan, RN 2005. Chitooligosaccharides – preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity. Carbohydrate Research 340, 1239–1245.CrossRefGoogle ScholarPubMed
Koide, SS 1998. Chitin–chitosan: properties, benefits and risks. Journal of Nutrition Research 18, 1091–1101.CrossRefGoogle Scholar
Lalles, J-P, Bosi, P, Smidt, H, Stokes, CR 2007. Nutritional management of gut health in pigs around weaning. Proceedings of the Nutrition Society 66, 260–268.CrossRefGoogle ScholarPubMed
Liu, H, Du, Y, Wang, X, Sun, L 2004. Chitosan kills bacteria through cell membrane damage. International Journal of Food Microbiology 95, 147–155.CrossRefGoogle ScholarPubMed
Liu, N, Chen, X-G, Park, H-J, Liu, C-G, Liu, C-S, Meng, X-H, Yu, L-J 2006. Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli . Journal of Carbohydrate Polymers 64, 60–65.CrossRefGoogle Scholar
Liu, P, Piao, XS, Kim, SW, Wang, L, Shen, YB, Lee, HS, Li, SY 2008. Effects of chito-oligosaccharide supplementation on the growth performance, nutrient digestibility, intestinal morphology, and fecal shedding of Escherichia coli and Lactobacillus in weaning pigs. Journal of Animal Science 86, 2609–2618.CrossRefGoogle ScholarPubMed
Liu, P, Piao, XS, Thacker, PA, Zeng, ZK, Li, PF, Wang, D, Kim, SW 2010. Chito-oligosaccharide reduces diarrhea incidence and attenuates the immune response of weaned pigs challenged with E. coli K88. Journal of Animal Science 88, 3871–3879.CrossRefGoogle Scholar
Macfarlane, S, Macfarlane, GT 2003. Regulation of short-chain fatty acid production. Proceedings of the Nutrition Society 62, 67–72.CrossRefGoogle ScholarPubMed
Mackie, RI, Stroot, PG, Varel, VH 1998. Biochemical identification and biological origin of key odor components in livestock waste. Journal of Animal Science 76, 1331–1342.CrossRefGoogle ScholarPubMed
Mori, T, Okumura, M, Matsuura, M, Ueno, K, Tokura, S, Okamoto, Y, Minami, S, Fujinaga, T 1997. Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro. Biomaterials 18, 947–951.CrossRefGoogle ScholarPubMed
Mourão, JL, Pinheiro, V, Alves, A, Guedes, CM, Pinto, L, Saavedra, MJ, Spring, P, Kocher, A 2006. Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Animal Feed Science and Technology 126, 107–120.CrossRefGoogle Scholar
National Research Council 1998. Nutrient requirements of swine. National Academy Press, Washington, DC.Google Scholar
No, HK, Young Park, N, Ho Lee, S, Meyers, SP 2002. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. International Journal of Food Microbiology 74, 65–72.CrossRefGoogle ScholarPubMed
O'Doherty, JV, Dillon, S, Figat, S, Callan, JJ, Sweeney, T 2010. The effects of lactose inclusion and seaweed extract derived from Laminaria spp. on performance, digestibility of diet components and microbial populations in newly weaned pigs. Animal Feed Science and Technology 157, 173–180.CrossRefGoogle Scholar
Ofek, I, Hasty, DL, Sharon, N 2003. Anti-adhesion therapy of bacterial diseases: prospects and problems. FEMS Immunology & Medical Microbiology 38, 181–191.CrossRefGoogle ScholarPubMed
Pierce, KM, Callan, JJ, McCarthy, P, O'Doherty, JV 2007. The interaction between lactose level and crude protein concentration on piglet post-weaning performance, nitrogen metabolism, selected faecal microbial populations and faecal volatile fatty acid concentrations. Animal Feed Science and Technology 132, 267–282.CrossRefGoogle Scholar
Pierce, KM, Sweeney, T, Brophy, PO, Callan, JJ, Fitzpatrick, E, McCarthy, P, O'Doherty, JV 2006. The effect of lactose and inulin on intestinal morphology, selected microbial populations and volatile fatty acid concentrations in the gastro-intestinal tract of the weanling pig. Journal of Animal Science 82, 311–318.CrossRefGoogle Scholar
Pluske, JR, Thompson, MJ, Atwood, CS, Bird, PH, Williams, IH, Hartmann, PE 1996. Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows’ whole milk after weaning. British Journal of Nutrition 76, 409–422.CrossRefGoogle ScholarPubMed
Qin, C, Li, H, Xiao, Q, Liu, Y, Zhu, J, Du, Y 2006. Water-solubility of chitosan and its antimicrobial activity. Journal of Carbohydrate Polymers 63, 367–374.CrossRefGoogle Scholar
Salanitro, JP, Blake, IG, Muirhead, PA 1977. Isolation and identification of fecal bacteria from adult swine. Applied and Environmental Microbiology 33, 79–84.CrossRefGoogle ScholarPubMed
SAS 2004. SAS users guide. SAS Institue Inc., Cary, NC.Google Scholar
Sauvant, D, Perez, JM, Tran, G 2004. Table of composition and nutritional value of feed materials: pigs, poultry, cattle, sheep, goats, rabbits, horses, fish. Wageningen Academic Publishers, the Netherlands.CrossRefGoogle Scholar
Spreeuwenberg, MAM, Verdonk, JMAJ, Gaskins, HR, Verstegen, MWA 2001. Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. The Journal of Nutrition 131, 1520–1527.CrossRefGoogle ScholarPubMed
Torzsas, TL, Kendall, CWC, Sugano, M, Iwamoto, Y, Rao, AV 1996. The influence of high and low molecular weight chitosan on colonic cell proliferation and aberrant crypt foci development in CF1 mice. Food and Chemical Toxicology 34, 73–77.CrossRefGoogle ScholarPubMed
van Beers-Schreurs, HMâG, Nabuurs, MJâA, Vellenga, L, van der Valk, HJK, Wensing, T, Breukink, HJ 1998. Weaning and the weanling diet influence the villous height and crypt depth in the small intestine of pigs and alter the concentrations of short-chain fatty acids in the large intestine and blood. The Journal of Nutrition 128, 947–953.CrossRefGoogle ScholarPubMed
Zheng, L-Y, Zhu, J-F 2003. Study on antimicrobial activity of chitosan with different molecular weights. Journal of Carbohydrate Polymers 54, 527–530.CrossRefGoogle Scholar