Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-23T05:22:22.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Developmental potential of oocytes fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) after cryopreservation and mesometrial autotransplantation of rabbit ovarian tissue

Published online by Cambridge University Press:  01 September 2008

C. Lan ,
D. Xiaohui ,
F. Qingzhao ,
X. Anran ,
H. Chun-yan ,
Y. Hongling  and
Y. Xuan
C. Lan
Affiliation:
Infertility Center, Qilu Hospital, Shandong University, Jinan, 250012, China
D. Xiaohui*
Affiliation:
Infertility Center, Qilu Hospital, Shandong University, Jinan, 250012, China
F. Qingzhao
Affiliation:
Infertility Center, Qilu Hospital, Shandong University, Jinan, 250012, China
X. Anran
Affiliation:
Infertility Center, Qilu Hospital, Shandong University, Jinan, 250012, China
H. Chun-yan
Affiliation:
Department of Pathology, Qilu Hospital, Shandong University, China
Y. Hongling
Affiliation:
Infertility Center, Qilu Hospital, Shandong University, Jinan, 250012, China
Y. Xuan
Affiliation:
Infertility Center, Qilu Hospital, Shandong University, Jinan, 250012, China
*
E-mail: [email protected]
Get access

Abstract

Objective: To evaluate mesometrial transplantation of frozen-thawed ovarian tissue in rabbit and to choose the optimized fertilization method for oocytes retrieved from grafts by investigating the capability of oocyte fertilization and further development. Forty rabbits were divided into three groups randomly: control group, fresh tissues transplantation group and frozen-thawed tissues transplantation group. Three months after the transplantation, rabbits were stimulated with FSH and oocytes were retrieved 13 h after human chorionic gonadotropin (HCG) injection. Oocytes matured in vivo or in vitro were then fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), followed by observation and evaluation of fertilization rate and blastocyst formation rate. Blastocytes embryos were transferred to pseudopregnancy rabbits to observe pregnancy rate and birth rate. There were no significant differences in the percentage of oocytes matured either in vivo or in vitro among the three groups. The fertilization rate, cleavage rate and blastocyst formation rate of in vivo-matured oocytes had no difference among the three groups, whether they were fertilized by IVF or ICSI. Significantly higher fertilization rates of in vitro-matured oocytes were observed with ICSI compared with IVF in each group. The blastocyst formation rate of in vitro-matured oocytes was significantly lower than that of in vivo-matured oocytes in each group. The birth rate of in vivo-matured oocytes was significantly higher than that of in vitro-matured oocytes, although the pregnancy rate was similar between them. Mesometrial transplantation of frozen-thawed ovarian tissue may provide favorable conditions for follicle development. Oocytes retrieved from mesometrial grafts can develop to the blastocyst stage and produce live offspring. ICSI can optimize the fertilization rate of in vitro-matured oocytes retrieved from grafts.

Keywords

ovarian tissuetransplantationfollicle developmentoocyte maturationin vitro fertilization
Type
Full Paper
Information
animal , Volume 2 , Issue 9 , September 2008 , pp. 1371 - 1376
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abir, R, Orvieto, R, Raanani, H, Fisch, B, Schoenfeld, A, Ginton, D, Nitke, S, Ben Rafael, Z 2000. Development of human fetal follicles in an immunodeficient mouse. Journal of Assisted Reproduction and Genetics 17, 393–396.Google Scholar
Al-Hasani, S, Trotnow, S, Sadtler, C, Hahn, J 1986. In vitro fertilization and embryo transfer of pre-ovulatory rabbit oocytes. European Journal of Obstetrics, Gynecology, and Reproductive Biology 21, 187–195.Google Scholar
Almodin, CG, Minguetti-Câmara, VC, Meister, H, Ferreira, JO, Franco, RL, Cavalcante, AA, Radaelli, MR, Bahls, AS, Moron, AF, Murta, CG 2004. Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy. Human Reproduction 19, 1287–1293.CrossRefGoogle ScholarPubMed
Aubard, Y, Piver, P, Cognie, Y, Fermeaux, V, Poulin, N, Driancourt, MA 1999. Orthotopic and heterotopic autografts of frozenthawed ovarian cortex in sheep. Human Reproduction 14, 2149–2154.CrossRefGoogle ScholarPubMed
Baird, DT, Webb, R, Campbell, BK, Harkness, LM, Gosden, RG 1999. Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at −196°C. Endocrinology 140, 462–471.Google Scholar
Carneiro, G, Lorenzo, P, Pimentel, C, Pegoraro, L, Bertolini, M, Ball, B, Anderson, G, Liu, I 2001. Influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol, and fetal calf serum on In vitro maturation and parthenogenic development in equine oocytes. Biology of Reproduction 65, 899–905.Google Scholar
Dell’Aquila, ME, Cho, YS, Minoia, P, Traina, V, Fusco, S, Lacalandra, GM, Maritato, F 1997. Intracytoplasmic sperm injection (ICSI) versus conventional IVF on abattoir-derived and in vitro-matured equine oocytes. Theriogenology 47, 1139–1156.CrossRefGoogle ScholarPubMed
Deng, M, Yang, XJ 2001. Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection. Molecular Reproduction and Development 59, 38–43.CrossRefGoogle ScholarPubMed
Deng, XH, Xu, AR, Chao, L, Yu, HL, Zhen, JH, Hashimoto, S, Morimoto, Y 2007. Effect of different sites for cryopreserved ovarian tissue implantation in rabbit. Human Reproduction 22, 662–668.CrossRefGoogle ScholarPubMed
Donnez, J, Dolmans, MM, Demylle, D, Jadoul, P, Pirard, C, Squifflet, J, Martinez-Madrid, B, Van Langendonckt, A 2004. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. The Lancet 364 9443, 1405–1410.Google Scholar
Fukuda, A, Roudebush, WE, Thatcher, SS 1992. Influences of in vitro oocyte aging on microfertilization in the mouse with reference to zona hardening. Journal of Assisted Reproduction and Genetics 9, 378–383.CrossRefGoogle ScholarPubMed
Gosden, RG, Baird, DT, Wade, JC, Webb, R 1994a. Restoration of fertility to oophorectomized sheep by ovarian autografts stored at −196 degrees C. Human Reproduction 9, 597–603.Google Scholar
Gosden, RG, Boulton, MI, Grant, K, Webb, R 1994b. Follicular development of ovarian xenografts in SCID mice. Journal of Reproduction and Fertility 101, 619–623.Google Scholar
Greenlee, RT, Hill-Harmon, MB, Murray, T, Thun, M 2001. Cancer statistics, 2001. CA: a cancer journal for clinicians 51, 15–36.Google ScholarPubMed
Jemal, A, Clegg, LX, Ward, E, Ries, LA, Wu, X, Jamison, PM, Wingo, PA, Howe, HL, Anderson, RN, Edwards, BK 2004. Annual report to the nation on the status of cancer, 1975–2001, with a special feature regarding survival. Cancer 101, 3–27.CrossRefGoogle Scholar
Kaneko, H, Kikuchi, K, Noguchi, J, Ozawa, M, Ohnuma, K, Maedomari, N, Kashiwazaki, N 2006. Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice. Reproduction 131, 279–288.Google Scholar
Kim, SS 2006. Fertility preservation in female cancer patients: current developments and future directions. Fertility and Sterility 85, 1–11.CrossRefGoogle ScholarPubMed
Kiran, G, Kiran, H, Coban, YK, Guven, AM, Yuksel, M 2004. Fresh autologous transplantation of ovarian cortical strip to the anterior abdominal wall at the pfannenstiel incision site. Fertility and Sterility 82, 954–956.CrossRefGoogle Scholar
Lechniak, D, Warzych, E, Pers-Kamczyc, E, Sosnowski, J, Antosik, P, Rubes, J 2007. Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly. Theriogenology 68, 755–762.Google Scholar
Lee, RK, Ho, HY, Yu, SL, Lu, CH 2005. Blastocyst development after cryopreservation and subcutaneous transplantation of mouse ovarian tissue. Journal of Assisted Reproduction and Genetics 22, 95–101.CrossRefGoogle ScholarPubMed
Liu, J, Van der Elst, J, Van den Broecke, R, Dhont, M 2001. Live offspring by in vitro fertilization of oocytes from cryopreserved primordial mouse follicles after sequential in vivo transplantation and in vitro maturation. Biology of Reproduction 64, 171–178.CrossRefGoogle ScholarPubMed
Liu, J, Rybouchkin, A, Van der Elat, J, Dhont, M 2002. Fertilization of mouse oocytes from in vitro-matured preantral follicles using classical in vitro fertilization or intracytoplasmic sperm injection. Biology of Reproduction 67, 575–579.CrossRefGoogle ScholarPubMed
Lorenzo, PL, Rebollar, PG, Illera, MJ, Illera, JC, Illera, M, Alvarino, JM 1996. Stimulatory effect of insulin-like growth factor I and epidermal growth factor on the maturation of rabbit oocytes in vitro. Journal of Reproduction and Fertility 107, 109–117.CrossRefGoogle ScholarPubMed
Mao, J, Smith, MF, Rucker, EB, Wu, GM, McCauley, TC, Cantley, TC, Prather, RS, Didion, BA, Day, BN 2004. Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro. Journal of Animal Science 82, 1967–1975.CrossRefGoogle ScholarPubMed
Meirow, D, Levron, J, Eldar-Geva, T, Hardan, I, Fridman, E, Yemini, Z, Dor, J 2007. Monitoring the ovaries after autotransplantation of cryopreserved ovarian tissue: endocrine studies, in vitro fertilization cycles, and live birth. Fertility and sterility 87, 418.e7–418.e15.CrossRefGoogle ScholarPubMed
Nisolle, M, Casanas-Roux, F, Qu, J, Motta, P, Donnez, J 2000. Histologic and ultrastructural evaluation of fresh and frozenthawed human ovarian xenografts in nude mice. Fertility and Sterility 74, 122–129.Google Scholar
Nogueira, D, Staessen, C, Van de Velde, H, Van Steirteghem, A 2000. Nuclear status and cytogenetics of embryos derived from in vitro-matured oocytes. Fertility and Sterility 74, 295–298.Google Scholar
Nugent, D, Newton, H, Gallivan, L, Gosden, RG 1998. Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. Journal of Reproduction and Fertility 114, 341–346.CrossRefGoogle ScholarPubMed
O’Brien, MJ, Pendola, JK, Eppig, JJ 2003. A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biology of Reproduction 68, 1682–1686.Google Scholar
Oktay, K, Newton, H, Mullan, J, Gosden, R 1998. Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone. Human Reproduction 13, 1133–1138.CrossRefGoogle ScholarPubMed
Oktay, K, Buyuk, E, Veeck, L, Zaninovic, N, Xu, K, Takeuchi, T, Opsahl, M, Rosenwaks, Z 2004. Embryo development after heterotopic transplantation of cryopreserved ovarian tissue. Lancet 363, 837–840.Google Scholar
Revel, F, Mermillod, P, Peynot, N, Renard, JP, Heyman, Y 1995. Low developmental capacity of in vitro matured and fertilized oocytes from calves compared with that of cows. Journal of Reproduction and Fertility 103, 115–120.CrossRefGoogle ScholarPubMed
Watanabe, N, Nakagawa, H, Kitajima, S, Liu, E, Morimoto, M, Watanabe, T, Fan, J 2005. Establishment of a SPF colony of human apo(a) transgenic rabbits by frozenthawed embryo transfer. Experimental Animals 54, 353–357.CrossRefGoogle ScholarPubMed
Waterhouse, T, Cox, SL, Snow, M, Jenkin, G, Shaw, J 2004. Offspring produced from heterotopic ovarian allografts in male and female recipient mice. Reproduction 127, 689–694.Google Scholar
Yang, HY, Cox, SL, Jenkin, G, Findlay, J, Trounson, A, Shaw, J 2006. Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts. Reproduction 131, 851–859.Google Scholar
Yoshimura, Y, Ando, M, Nagamatsu, S, Iwashita, M, Adachi, T, Sueoka, K, Miyazaki, T, Kuji, N, Tanaka, M 1996. Effects of insulin-like growth factor-I on follicle growth, oocyte maturation, and ovarian steroidogenesis and plasminogen activator activity in the rabbit. Biology of Reproduction 55, 152–160.Google Scholar
Zeng, SM, Zhu, SE, Wang, YS, Chen, XI, Zhang, ZC, Chen, YF 1999. An efficient method for in vitro fertilization in rabbits. Animal Biotechnology 10, 15–23.CrossRefGoogle ScholarPubMed
Zygmunt, M, Herr, F, Keller-Schoenwetter, S, Kunzi-Rapp, K, Munstedt, K, Rao, CV, Lang, U, Preissner, KT 2002. Characterization of human chorionic gonadotropin as a novel angiogenic factor. The Journal of Clinical Endocrinology and Metabolism 87, 5290–5296.CrossRefGoogle ScholarPubMed