Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-20T01:29:02.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics

Published online by Cambridge University Press:  16 October 2008

C. Jurie ,
B. Picard ,
Y. Heyman ,
I. Cassar-Malek ,
P. Chavatte-Palmer ,
C. Richard  and
J. F. Hocquette
C. Jurie*
Affiliation:
INRA, UR1213 Herbivores, Centre de Clermont-Ferrand/Theix, F-63122 Saint Genès Champanelle, France
B. Picard
Affiliation:
INRA, UR1213 Herbivores, Centre de Clermont-Ferrand/Theix, F-63122 Saint Genès Champanelle, France
Y. Heyman
Affiliation:
INRA, UMR1198, Biologie du Développement et Reproduction, F-78352 Jouy en Josas, France
I. Cassar-Malek
Affiliation:
INRA, UR1213 Herbivores, Centre de Clermont-Ferrand/Theix, F-63122 Saint Genès Champanelle, France
P. Chavatte-Palmer
Affiliation:
INRA, UMR1198, Biologie du Développement et Reproduction, F-78352 Jouy en Josas, France
C. Richard
Affiliation:
INRA, Domaine expérimental de Bressonvilliers, F-91630 Leudeville, France
J. F. Hocquette
Affiliation:
INRA, UR1213 Herbivores, Centre de Clermont-Ferrand/Theix, F-63122 Saint Genès Champanelle, France
*
E-mail: [email protected]
Get access

Abstract

Muscle contractile and metabolic characteristics were studied on nine cloned and eight non-cloned (control) heifers. The animals were submitted to repeated biopsies of the semitendinosus (ST) muscle at the ages of 8, 12, 18 and 24 months. The contractile type was determined from the proportion of the different myosin heavy chain (MyHC) isoforms separated by electrophoresis. Glycolytic metabolism was assessed by lactate dehydrogenase (LDH) activity, and oxidative metabolism was assessed by isocitrate dehydrogenase (ICDH), cytochrome-c oxidase (COX) and β-hydroxyacyl-CoA dehydrogenase (HAD) activities. In cloned heifers at 8 months of age, there was a greater proportion of MyHC I (slow oxidative isoform) and MyHC IIa (fast oxido-glycolytic isoform), a lower proportion of MyHC IIx (fast glycolytic isoform), greater COX and HAD activity and a lower LDH/ICDH ratio compared with control heifers. Thus, young cloned heifers had slower muscle types associated with a more oxidative muscular metabolism than control heifers. From 12 months of age onwards, no significant differences were observed between cloned and control heifers. A delay in muscle differentiation and maturation in cloned heifers is hypothesised and discussed.

Keywords

bovineclonemetabolismmusclemyosin heavy chain
Type
Full Paper
Information
animal , Volume 3 , Issue 2 , February 2009 , pp. 244 - 250
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Berthelot V, Bas P, Heyman Y and Chavatte-Palmer P 2004. Comparison of fatty acid composition of muscle and milk of Holstein cows cloned by nuclear transfer with non-cloned cows. 11ème Rencontres Recherches Ruminants, Institut de l’Elevage, INRA, Paris, France, 394pp.Google Scholar
Chikuni, K, Muroya, S, Nakajima, I 2004. Myosin heavy chain isoforms expressed in bovine skeletal muscles. Meat Science 67, 87–94.CrossRefGoogle ScholarPubMed
Cibelli, JB, Stice, S, Golueke, PJ, Kane, JJ, Jerry, J, Blackwell, C, Ponce de Leon, FA, Robl, JL 1998. Cloned transgenic calves produced from non quiescent fetal fibroblasts. Science 280, 1256–1258.CrossRefGoogle Scholar
Geay, Y, Bauchart, D, Hocquette, JF, Culioli, J 2001. Effect of nutritional factors on biochemical, structural and metabolic characteristics of muscles in ruminants, consequences on dietetic value and sensorial qualities of meat. Reproduction Nutrition Development 41, 1–26.CrossRefGoogle ScholarPubMed
Heyman, Y, Chavatte-Palmer, P, Berthelot, V, Fromentin, G, Hocquette, JF, Martignat, L, Renard, JP 2007a. Assessing the quality of products from cloned cattle: an integrative approach. Theriogenology 67, 134–141.CrossRefGoogle ScholarPubMed
Heyman, Y, Chavatte-Palmer, P, Fromentin, G, Berthelot, V, Jurie, C, Bas, P, Dubarry, M, Mialot, JP, Remy, D, Richard, C, Martignat, L, Vignon, X, Renard, JP 2007b. Quality and safety of bovine clones and their products. Animal 1, 963–972.CrossRefGoogle ScholarPubMed
Hocquette, JF, Ortigues-Marty, I, Pethick, D, Herpin, P, Fernandez, X 1998. Nutritional and hormonal regulation of energy metabolism in skeletal muscles of meat-producing animals. Livestock Production Science 56, 115–143.CrossRefGoogle Scholar
Jurie, C, Robelin, J, Picard, B, Renand, G, Geay, Y 1995. Postnatal changes in the biological characteristics of semitendinosus muscle in male Limousin cattle. Meat Science 41, 125–135.CrossRefGoogle ScholarPubMed
Jurie, C, Martin, JF, Listrat, A, Jailler, R, Culioli, J, Picard, B 2005. Effects of age and breed of beef bulls on growth parameters, carcass and muscle characteristics. Animal Science 80, 257–263.CrossRefGoogle Scholar
Jurie, C, Ortigues-Marty, I, Picard, B, Micol, D, Hocquette, JF 2006. The separate effects of the nature of diet and grazing mobility on metabolic potential of muscles from Charolais steers. Livestock Science 104, 182–192.CrossRefGoogle Scholar
Klont, RE, Brocks, L, Eikelenboom, G 1998. Muscle fire type and meat quality. Meat Science 49 (suppl. 1), 219–229.CrossRefGoogle Scholar
Lengyel, Z, Husvéth, F, Polgár, P, Szabó, F, Magyar, L 2003. Fatty acid composition of intramuscular lipids in various muscles of Holstein-Friesan bulls slaughtered at different ages. Meat Science 65, 593–598.CrossRefGoogle Scholar
Littell, RC, Henry, PR, Ammerman, CB 1998. Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76, 1216–1231.CrossRefGoogle Scholar
Maltin, CA, Sinclair, KD, Warriss, PD, Grant, CM, Porter, AD, Delday, MI, Warkup, CC 1998. The effects of age at slaughter, genotype and finishing system on the biochemical properties, muscle fibre type characteristics and eating quality of bull beef from suckled calves. Animal Science 66, 341–348.CrossRefGoogle Scholar
Maltin, CA, Balcerzak, D, Tilley, R, Delday, M 2003. Determinants of meat quality: tenderness. Proceedings of the Nutrition Society 62, 337–347.CrossRefGoogle ScholarPubMed
Picard, B, Barboiron, C, Duris, MP, Gagnière, H, Jurie, C, Geay, Y 1999. Electrophoretic separation of bovine muscle myosin heavy chain isoforms. Meat Science 53, 1–7.CrossRefGoogle ScholarPubMed
Picard, B, Lefaucheur, L, Berri, C, Duclos, MJ 2002. Muscle fibre ontogenesis in farm animal species. Reproduction Nutrition Development 42, 415–431.CrossRefGoogle ScholarPubMed
Picard, B, Jurie, C, Duris, MP, Renand, G 2006a. Consequences of selection for higher growth rate on muscle fibre development in cattle. Livestock Science 102, 107–120.CrossRefGoogle Scholar
Picard B, Jurie C, Laigre P, Heyman Y, Vignon X, Cassar-Malek I, Hocquette JF and Chavatte-Palmer P 2006b. Muscle development of cloned cattle foetuses. Meeting COST 925, Antalya, Turkey.Google Scholar
Takahashi, S, Ito, Y 2004. Evaluation of meat products from cloned cattle: biological and biochemical properties. Cloning and Stem Cells 6, 165–171.CrossRefGoogle ScholarPubMed
Tian, XC, Kubota, C, Sakashita, K, Izaike, Y, Okano, R, Tabara, N, Curchoe, C, Jacob, L, Zhang, Y, Smith, S, Bormann, C, Xu, J, Sato, M, Andrew, S, Yang, X 2005. Meat and milk compositions of bovine clones. Proceedings of the National Academy of Sciences of the United States of America 102, 6261–6266.CrossRefGoogle ScholarPubMed
Vignon, X, Chesné, P, Lebourhis, D, Fléchon, JE, Heyman, Y, Renard, JP 1998. Developmental potential of bovine embryos reconstructed with enucleated matured oocytes fused with cultured somatic cells. Comptes Rendus de l’Académie des Sciences. Série 3, Sciences de la Vie 321, 735–745.Google ScholarPubMed
Wegner, J, Albrecht, E, Fiedler, I, Teuscher, F, Papstein, HJ, Ender, K 2000. Growth- and breed-related changes of muscle fiber characteristics in cattle. Journal of Animal Science 78, 1485–1496.CrossRefGoogle ScholarPubMed
Yamaguchi, M, Ito, Y, Takahashi, S 2007. Fourteen-week feeding test of meat and milk derived from cloned cattle in the rat. Theriogenology 67, 152–165.CrossRefGoogle ScholarPubMed